Extended-spectrum ?-lactamase (ESBL)-producing enterobacteria isolated from broiler breeder chickens
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Biotemas (Online) |
Texto Completo: | https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2019v32n4p1 |
Resumo: | Due to the use of antibiotics for the treatment and prevention of bacterial infections in intensive poultry production, it is important to study the prevalence and resistance profile of gram-negative extended-spectrum beta-lactamase (ESBL)-producing bacteria. Among the several classes of antibiotics available, beta-lactam agents are the most widely used category in human and veterinary medicine. Thus, the objective of this work was to evaluate the resistance profile and ESBL-producing capacity of strains isolated from broiler breeders. Gram-negative bacteria were isolated in selective media from a collection of 87 cloacal swabs, and they were identified by conventional biochemical methods and MALDI-TOF mass spectrometry and studied for antibiotic resistance, including ESBL production. Of the 87 samples, 52 gram-negative bacteria were isolated. There was high bacterial resistance to the main classes of antibiotics used in poultry: cephalosporins (74.2%), quinolones (73.8%) and penicillins (73.6%). A phenotype suggestive of ESBL production was also found in 42.31% of isolates, showing resistance to the beta-lactam antibiotics tested. The results demonstrate the need for the guidance of professionals working broiler breeders to reduce the presence of drug-resistant and ESBL-producing bacteria and to prevent their spread to the environment, fertile eggs and broilers, and consequently to chicken meat and humans. |
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Extended-spectrum ?-lactamase (ESBL)-producing enterobacteria isolated from broiler breeder chickensEnterobactérias produtoras de ?-lactamases de espectro estendido (ESBL) isoladas de matrizes de frangos de corteDue to the use of antibiotics for the treatment and prevention of bacterial infections in intensive poultry production, it is important to study the prevalence and resistance profile of gram-negative extended-spectrum beta-lactamase (ESBL)-producing bacteria. Among the several classes of antibiotics available, beta-lactam agents are the most widely used category in human and veterinary medicine. Thus, the objective of this work was to evaluate the resistance profile and ESBL-producing capacity of strains isolated from broiler breeders. Gram-negative bacteria were isolated in selective media from a collection of 87 cloacal swabs, and they were identified by conventional biochemical methods and MALDI-TOF mass spectrometry and studied for antibiotic resistance, including ESBL production. Of the 87 samples, 52 gram-negative bacteria were isolated. There was high bacterial resistance to the main classes of antibiotics used in poultry: cephalosporins (74.2%), quinolones (73.8%) and penicillins (73.6%). A phenotype suggestive of ESBL production was also found in 42.31% of isolates, showing resistance to the beta-lactam antibiotics tested. The results demonstrate the need for the guidance of professionals working broiler breeders to reduce the presence of drug-resistant and ESBL-producing bacteria and to prevent their spread to the environment, fertile eggs and broilers, and consequently to chicken meat and humans.Devido ao uso de antibióticos para o tratamento e prevenção de infecções bacterianas durante a produção de matrizes pesadas, torna-se importante estudar o perfil de resistência e a prevalência de bactérias gram-negativas produtoras de ESBL. Dentre as várias classes de antimicrobianos disponíveis, destacam-se os agentes beta-lactâmicos, categoria antimicrobiana amplamente utilizada em clínica médica humana e veterinária. Assim, este trabalho teve como objetivo avaliar o perfil de resistência e caracterizar as cepas produtoras de beta-lactamases de espectro estendido (ESBL) isoladas de matrizes de corte por meio da coleta de 87 swabs cloacais. As bactérias Gram-negativas foram isoladas em meios seletivos, identificadas por métodos bioquímicos convencionais e MALDI-TOF® e estudadas quanto à resistência antimicrobiana, incluindo ESBL. Das 87 amostras, foram isoladas 52 bactérias gram-negativas. Houve alta resistência bacteriana às principais classes de antimicrobianos utilizados em aves: penicilinas, cefalosporinas e quinolonas. Também foi encontrado fenótipo sugestivo de produção de ESBL em 42,31% dos isolados, demonstrando resistência aos antimicrobianos beta-lactâmicos testados. Os resultados demonstram a necessidade de orientação dos profissionais que trabalham com matrizes de corte, a fim de se reduzir a presença de bactérias resistentes e produtoras de ESBL e evitar sua disseminação para o meio ambiente, ovos férteis e frangos de corte e, consequentemente, carne de frango e humanos.Universidade Federal de Santa Catarina2019-12-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2019v32n4p110.5007/2175-7925.2019v32n4p1Biotemas; v. 32 n. 4 (2019); 1-102175-79250103-1643reponame:Biotemas (Online)instname:Universidade Federal de Santa Catarina (UFSC)instacron:UFSCenghttps://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2019v32n4p1/42046Copyright (c) 2019 Taniara Suelen Mezalira, Daniela Dib Gonçalves, Eduardo Herrera Dias, Fábio Mendes da Silva, Isabela Carvalho dos Santos, Edson Gerônimo, Jaciele Caroline Pereira Dias, Luciana Kazue Otutumiinfo:eu-repo/semantics/openAccessMezalira, Taniara SuelenGonçalves, Daniela DibDias, Eduardo HerreraSilva, Fábio Mendes daSantos, Isabela Carvalho dosGerônimo, EdsonDias, Jaciele Caroline PereiraOtutumi, Luciana Kazue2019-12-12T07:56:23Zoai:periodicos.ufsc.br:article/62068Revistahttp://www.biotemas.ufsc.br/index.htmPUBhttps://periodicos.ufsc.br/index.php/biotemas/oai||carlospinto@ccb.ufsc.br2175-79250103-1643opendoar:2019-12-12T07:56:23Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC)false |
dc.title.none.fl_str_mv |
Extended-spectrum ?-lactamase (ESBL)-producing enterobacteria isolated from broiler breeder chickens Enterobactérias produtoras de ?-lactamases de espectro estendido (ESBL) isoladas de matrizes de frangos de corte |
title |
Extended-spectrum ?-lactamase (ESBL)-producing enterobacteria isolated from broiler breeder chickens |
spellingShingle |
Extended-spectrum ?-lactamase (ESBL)-producing enterobacteria isolated from broiler breeder chickens Mezalira, Taniara Suelen |
title_short |
Extended-spectrum ?-lactamase (ESBL)-producing enterobacteria isolated from broiler breeder chickens |
title_full |
Extended-spectrum ?-lactamase (ESBL)-producing enterobacteria isolated from broiler breeder chickens |
title_fullStr |
Extended-spectrum ?-lactamase (ESBL)-producing enterobacteria isolated from broiler breeder chickens |
title_full_unstemmed |
Extended-spectrum ?-lactamase (ESBL)-producing enterobacteria isolated from broiler breeder chickens |
title_sort |
Extended-spectrum ?-lactamase (ESBL)-producing enterobacteria isolated from broiler breeder chickens |
author |
Mezalira, Taniara Suelen |
author_facet |
Mezalira, Taniara Suelen Gonçalves, Daniela Dib Dias, Eduardo Herrera Silva, Fábio Mendes da Santos, Isabela Carvalho dos Gerônimo, Edson Dias, Jaciele Caroline Pereira Otutumi, Luciana Kazue |
author_role |
author |
author2 |
Gonçalves, Daniela Dib Dias, Eduardo Herrera Silva, Fábio Mendes da Santos, Isabela Carvalho dos Gerônimo, Edson Dias, Jaciele Caroline Pereira Otutumi, Luciana Kazue |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Mezalira, Taniara Suelen Gonçalves, Daniela Dib Dias, Eduardo Herrera Silva, Fábio Mendes da Santos, Isabela Carvalho dos Gerônimo, Edson Dias, Jaciele Caroline Pereira Otutumi, Luciana Kazue |
description |
Due to the use of antibiotics for the treatment and prevention of bacterial infections in intensive poultry production, it is important to study the prevalence and resistance profile of gram-negative extended-spectrum beta-lactamase (ESBL)-producing bacteria. Among the several classes of antibiotics available, beta-lactam agents are the most widely used category in human and veterinary medicine. Thus, the objective of this work was to evaluate the resistance profile and ESBL-producing capacity of strains isolated from broiler breeders. Gram-negative bacteria were isolated in selective media from a collection of 87 cloacal swabs, and they were identified by conventional biochemical methods and MALDI-TOF mass spectrometry and studied for antibiotic resistance, including ESBL production. Of the 87 samples, 52 gram-negative bacteria were isolated. There was high bacterial resistance to the main classes of antibiotics used in poultry: cephalosporins (74.2%), quinolones (73.8%) and penicillins (73.6%). A phenotype suggestive of ESBL production was also found in 42.31% of isolates, showing resistance to the beta-lactam antibiotics tested. The results demonstrate the need for the guidance of professionals working broiler breeders to reduce the presence of drug-resistant and ESBL-producing bacteria and to prevent their spread to the environment, fertile eggs and broilers, and consequently to chicken meat and humans. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-12 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2019v32n4p1 10.5007/2175-7925.2019v32n4p1 |
url |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2019v32n4p1 |
identifier_str_mv |
10.5007/2175-7925.2019v32n4p1 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2019v32n4p1/42046 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
dc.source.none.fl_str_mv |
Biotemas; v. 32 n. 4 (2019); 1-10 2175-7925 0103-1643 reponame:Biotemas (Online) instname:Universidade Federal de Santa Catarina (UFSC) instacron:UFSC |
instname_str |
Universidade Federal de Santa Catarina (UFSC) |
instacron_str |
UFSC |
institution |
UFSC |
reponame_str |
Biotemas (Online) |
collection |
Biotemas (Online) |
repository.name.fl_str_mv |
Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC) |
repository.mail.fl_str_mv |
||carlospinto@ccb.ufsc.br |
_version_ |
1799940554484613120 |