In vitro propagation of sacaca (Croton cajucara): insights about difficulties for developing micropropagation protocols
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Biotemas (Online) |
Texto Completo: | https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n4p41 |
Resumo: | Sacaca is a medicinal plant from the Amazonian biome and it has been regarded as a substitute for rosewood (Aniba roseaodora) to produce linalool. This paper aimed to evaluate in vitro vegetative propagation of sacaca, including the establishment of propagules from the field, decontamination protocols, and determination of multiplication rates, besides describing limiting aspects for the culture during in vitro experiments. We used 1.0 cm microcuttings with an axillary bud, collected from adult plants in the field. Disinfestation treatments were tested in the establishment, and there is an evaluation of the collecting month influence on the contamination rates. After disinfestation, microcuttings were placed in test tubes containing MS medium, added with BAP (0, 1, 2 and 3 mg L-1) and GA3 (0 and 0.5 mg L-1). In vitro establishment of sacaca with 41.9% of sprouted microcuttings was obtained. The contamination rate reached 58.1% (65.4% caused by fungi and 34.6% by bacteria), with greater occurrence when propagules were collected between October and January, the rainiest months in the Amazon region. The increased BAP and GA3 concentrations in the culture medium provided significant improvements in the material multiplication rates. In spite of the results obtained, the species shows peculiarities and limitations to in vitro cultivation that were identified and described in this paper. |
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In vitro propagation of sacaca (Croton cajucara): insights about difficulties for developing micropropagation protocolsPropagação in vitro de sacaca (Croton cajucara Benth.): entendimentos sobre a dificuldade no desenvolvimento de protocolos de micropropagação da espécieSacaca is a medicinal plant from the Amazonian biome and it has been regarded as a substitute for rosewood (Aniba roseaodora) to produce linalool. This paper aimed to evaluate in vitro vegetative propagation of sacaca, including the establishment of propagules from the field, decontamination protocols, and determination of multiplication rates, besides describing limiting aspects for the culture during in vitro experiments. We used 1.0 cm microcuttings with an axillary bud, collected from adult plants in the field. Disinfestation treatments were tested in the establishment, and there is an evaluation of the collecting month influence on the contamination rates. After disinfestation, microcuttings were placed in test tubes containing MS medium, added with BAP (0, 1, 2 and 3 mg L-1) and GA3 (0 and 0.5 mg L-1). In vitro establishment of sacaca with 41.9% of sprouted microcuttings was obtained. The contamination rate reached 58.1% (65.4% caused by fungi and 34.6% by bacteria), with greater occurrence when propagules were collected between October and January, the rainiest months in the Amazon region. The increased BAP and GA3 concentrations in the culture medium provided significant improvements in the material multiplication rates. In spite of the results obtained, the species shows peculiarities and limitations to in vitro cultivation that were identified and described in this paper.A sacaca é uma planta medicinal do bioma Amazônico e tem sido considerada como substituta do pau-rosa (Aniba roseodora) na produção de linalol. Este trabalho objetivou avaliar a propagação vegetativa in vitro de sacaca, incluindo o estabelecimento de propágulos do campo, protocolos de descontaminação e a determinação de taxas de multiplicação, além de descrever aspectos limitantes da cultura durante os trabalhos in vitro. Foram utilizadas microestacas de 1,0 cm, com uma gema axilar, coletadas de plantas adultas do campo. Tratamentos de desinfestação foram testados no estabelecimento, além de se avaliar a influência do mês do ano da coleta sobre as taxas de contaminação. Após desinfestadas, as microestacas foram inoculadas em tubos de ensaio com meio MS, suplementado com BAP (0, 1, 2 e 3 mg L-1) e AG3 (0 e 0,5 mg L-1). Foi obtido o estabelecimento in vitro da sacaca com 41,9% das microestacas brotadas. A taxa de contaminação alcançou 58,1% (65,4% de origem fúngica e 34,6% de origem bacteriana), com maior ocorrência quando os propágulos foram coletados entre os meses de outubro e janeiro, os mais chuvosos da região amazônica. O aumento nas concentrações de BAP e AG3 no meio de cultura melhorou as taxas de multiplicação do material. Apesar dos resultados obtidos, a espécie apresenta uma série de peculiaridades e limitações ao cultivo in vitro que foram identificadas e relatadas neste trabalho. Universidade Federal de Santa Catarina2015-09-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n4p4110.5007/2175-7925.2015v28n4p41Biotemas; v. 28 n. 4 (2015); 41-502175-79250103-1643reponame:Biotemas (Online)instname:Universidade Federal de Santa Catarina (UFSC)instacron:UFSCporhttps://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n4p41/30439Copyright (c) 2015 Tatiane Loureiro da Silva, Maria Aparecida Alves Pereira, Jonny Everson Scherwinski-Pereirainfo:eu-repo/semantics/openAccessSilva, Tatiane Loureiro daPereira, Maria Aparecida AlvesScherwinski-Pereira, Jonny Everson2017-10-19T15:57:22Zoai:periodicos.ufsc.br:article/37802Revistahttp://www.biotemas.ufsc.br/index.htmPUBhttps://periodicos.ufsc.br/index.php/biotemas/oai||carlospinto@ccb.ufsc.br2175-79250103-1643opendoar:2017-10-19T15:57:22Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC)false |
dc.title.none.fl_str_mv |
In vitro propagation of sacaca (Croton cajucara): insights about difficulties for developing micropropagation protocols Propagação in vitro de sacaca (Croton cajucara Benth.): entendimentos sobre a dificuldade no desenvolvimento de protocolos de micropropagação da espécie |
title |
In vitro propagation of sacaca (Croton cajucara): insights about difficulties for developing micropropagation protocols |
spellingShingle |
In vitro propagation of sacaca (Croton cajucara): insights about difficulties for developing micropropagation protocols Silva, Tatiane Loureiro da |
title_short |
In vitro propagation of sacaca (Croton cajucara): insights about difficulties for developing micropropagation protocols |
title_full |
In vitro propagation of sacaca (Croton cajucara): insights about difficulties for developing micropropagation protocols |
title_fullStr |
In vitro propagation of sacaca (Croton cajucara): insights about difficulties for developing micropropagation protocols |
title_full_unstemmed |
In vitro propagation of sacaca (Croton cajucara): insights about difficulties for developing micropropagation protocols |
title_sort |
In vitro propagation of sacaca (Croton cajucara): insights about difficulties for developing micropropagation protocols |
author |
Silva, Tatiane Loureiro da |
author_facet |
Silva, Tatiane Loureiro da Pereira, Maria Aparecida Alves Scherwinski-Pereira, Jonny Everson |
author_role |
author |
author2 |
Pereira, Maria Aparecida Alves Scherwinski-Pereira, Jonny Everson |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Silva, Tatiane Loureiro da Pereira, Maria Aparecida Alves Scherwinski-Pereira, Jonny Everson |
description |
Sacaca is a medicinal plant from the Amazonian biome and it has been regarded as a substitute for rosewood (Aniba roseaodora) to produce linalool. This paper aimed to evaluate in vitro vegetative propagation of sacaca, including the establishment of propagules from the field, decontamination protocols, and determination of multiplication rates, besides describing limiting aspects for the culture during in vitro experiments. We used 1.0 cm microcuttings with an axillary bud, collected from adult plants in the field. Disinfestation treatments were tested in the establishment, and there is an evaluation of the collecting month influence on the contamination rates. After disinfestation, microcuttings were placed in test tubes containing MS medium, added with BAP (0, 1, 2 and 3 mg L-1) and GA3 (0 and 0.5 mg L-1). In vitro establishment of sacaca with 41.9% of sprouted microcuttings was obtained. The contamination rate reached 58.1% (65.4% caused by fungi and 34.6% by bacteria), with greater occurrence when propagules were collected between October and January, the rainiest months in the Amazon region. The increased BAP and GA3 concentrations in the culture medium provided significant improvements in the material multiplication rates. In spite of the results obtained, the species shows peculiarities and limitations to in vitro cultivation that were identified and described in this paper. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09-25 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n4p41 10.5007/2175-7925.2015v28n4p41 |
url |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n4p41 |
identifier_str_mv |
10.5007/2175-7925.2015v28n4p41 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n4p41/30439 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
dc.source.none.fl_str_mv |
Biotemas; v. 28 n. 4 (2015); 41-50 2175-7925 0103-1643 reponame:Biotemas (Online) instname:Universidade Federal de Santa Catarina (UFSC) instacron:UFSC |
instname_str |
Universidade Federal de Santa Catarina (UFSC) |
instacron_str |
UFSC |
institution |
UFSC |
reponame_str |
Biotemas (Online) |
collection |
Biotemas (Online) |
repository.name.fl_str_mv |
Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC) |
repository.mail.fl_str_mv |
||carlospinto@ccb.ufsc.br |
_version_ |
1799940553578643456 |