Extraction and recovery technique for myxozoan parasites from the Piaractus mesopotamicus kidney embedded in paraffin
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Biotemas (Online) |
Texto Completo: | https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2013v26n4p263 |
Resumo: | Tissues fixed and embedded in paraffin for histopathological studies keep their cell characteristics. There are several protocols for extracting genetic material from tissue embedded in paraffin, but there is no protocol for material aimed at the direct identification of parasites. The lack of techniques which describe the recovery of parasites from tissue embedded in paraffin has led us to test a technique for recovering myxosporean parasites found in Piaractus mesopotamicus kidney fragments embedded in paraffin, for a rapid, direct, and economic identification. Once the excess paraffin was removed from the kidney fragment, this was deparaffinized in xylene, hydrated in 70% alcohol, placed in an Eppendorf tube containing 70% alcohol, and left under vigorous and constant agitation in a vortex until the tissue was disintegrated. The precipitated material was mixed with the 70% alcohol and 20 µL were collected for preparing the smears, which were stained with Giemsa. Myxobolus sp. spores at many developmental stages were observed by light microscopy. The technique has proved to be useful for recovering myxosporean parasites from tissue embedded in paraffin and it constitutes an effective tool for prevalence studies when the myxosporean parasites are not detected in fresh mounts. |
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Extraction and recovery technique for myxozoan parasites from the Piaractus mesopotamicus kidney embedded in paraffinTissues fixed and embedded in paraffin for histopathological studies keep their cell characteristics. There are several protocols for extracting genetic material from tissue embedded in paraffin, but there is no protocol for material aimed at the direct identification of parasites. The lack of techniques which describe the recovery of parasites from tissue embedded in paraffin has led us to test a technique for recovering myxosporean parasites found in Piaractus mesopotamicus kidney fragments embedded in paraffin, for a rapid, direct, and economic identification. Once the excess paraffin was removed from the kidney fragment, this was deparaffinized in xylene, hydrated in 70% alcohol, placed in an Eppendorf tube containing 70% alcohol, and left under vigorous and constant agitation in a vortex until the tissue was disintegrated. The precipitated material was mixed with the 70% alcohol and 20 µL were collected for preparing the smears, which were stained with Giemsa. Myxobolus sp. spores at many developmental stages were observed by light microscopy. The technique has proved to be useful for recovering myxosporean parasites from tissue embedded in paraffin and it constitutes an effective tool for prevalence studies when the myxosporean parasites are not detected in fresh mounts.Universidade Federal de Santa Catarina2013-09-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2013v26n4p26310.5007/2175-7925.2013v26n4p263Biotemas; v. 26 n. 4 (2013); 263-2662175-79250103-1643reponame:Biotemas (Online)instname:Universidade Federal de Santa Catarina (UFSC)instacron:UFSCenghttps://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2013v26n4p263/25725Copyright (c) 2013 Wilson Gomez Manrique, Mayra Araguaia Pereira Figueiredo, Gustavo da Silva Claudiano, Maurício Laterça Martins, Flávio Ruas Moraesinfo:eu-repo/semantics/openAccessGomez Manrique, WilsonPereira Figueiredo, Mayra AraguaiaClaudiano, Gustavo da SilvaMartins, Maurício LaterçaMoraes, Flávio Ruas2017-10-20T17:18:16Zoai:periodicos.ufsc.br:article/29701Revistahttp://www.biotemas.ufsc.br/index.htmPUBhttps://periodicos.ufsc.br/index.php/biotemas/oai||carlospinto@ccb.ufsc.br2175-79250103-1643opendoar:2017-10-20T17:18:16Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC)false |
dc.title.none.fl_str_mv |
Extraction and recovery technique for myxozoan parasites from the Piaractus mesopotamicus kidney embedded in paraffin |
title |
Extraction and recovery technique for myxozoan parasites from the Piaractus mesopotamicus kidney embedded in paraffin |
spellingShingle |
Extraction and recovery technique for myxozoan parasites from the Piaractus mesopotamicus kidney embedded in paraffin Gomez Manrique, Wilson |
title_short |
Extraction and recovery technique for myxozoan parasites from the Piaractus mesopotamicus kidney embedded in paraffin |
title_full |
Extraction and recovery technique for myxozoan parasites from the Piaractus mesopotamicus kidney embedded in paraffin |
title_fullStr |
Extraction and recovery technique for myxozoan parasites from the Piaractus mesopotamicus kidney embedded in paraffin |
title_full_unstemmed |
Extraction and recovery technique for myxozoan parasites from the Piaractus mesopotamicus kidney embedded in paraffin |
title_sort |
Extraction and recovery technique for myxozoan parasites from the Piaractus mesopotamicus kidney embedded in paraffin |
author |
Gomez Manrique, Wilson |
author_facet |
Gomez Manrique, Wilson Pereira Figueiredo, Mayra Araguaia Claudiano, Gustavo da Silva Martins, Maurício Laterça Moraes, Flávio Ruas |
author_role |
author |
author2 |
Pereira Figueiredo, Mayra Araguaia Claudiano, Gustavo da Silva Martins, Maurício Laterça Moraes, Flávio Ruas |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Gomez Manrique, Wilson Pereira Figueiredo, Mayra Araguaia Claudiano, Gustavo da Silva Martins, Maurício Laterça Moraes, Flávio Ruas |
description |
Tissues fixed and embedded in paraffin for histopathological studies keep their cell characteristics. There are several protocols for extracting genetic material from tissue embedded in paraffin, but there is no protocol for material aimed at the direct identification of parasites. The lack of techniques which describe the recovery of parasites from tissue embedded in paraffin has led us to test a technique for recovering myxosporean parasites found in Piaractus mesopotamicus kidney fragments embedded in paraffin, for a rapid, direct, and economic identification. Once the excess paraffin was removed from the kidney fragment, this was deparaffinized in xylene, hydrated in 70% alcohol, placed in an Eppendorf tube containing 70% alcohol, and left under vigorous and constant agitation in a vortex until the tissue was disintegrated. The precipitated material was mixed with the 70% alcohol and 20 µL were collected for preparing the smears, which were stained with Giemsa. Myxobolus sp. spores at many developmental stages were observed by light microscopy. The technique has proved to be useful for recovering myxosporean parasites from tissue embedded in paraffin and it constitutes an effective tool for prevalence studies when the myxosporean parasites are not detected in fresh mounts. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09-12 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2013v26n4p263 10.5007/2175-7925.2013v26n4p263 |
url |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2013v26n4p263 |
identifier_str_mv |
10.5007/2175-7925.2013v26n4p263 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2013v26n4p263/25725 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
dc.source.none.fl_str_mv |
Biotemas; v. 26 n. 4 (2013); 263-266 2175-7925 0103-1643 reponame:Biotemas (Online) instname:Universidade Federal de Santa Catarina (UFSC) instacron:UFSC |
instname_str |
Universidade Federal de Santa Catarina (UFSC) |
instacron_str |
UFSC |
institution |
UFSC |
reponame_str |
Biotemas (Online) |
collection |
Biotemas (Online) |
repository.name.fl_str_mv |
Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC) |
repository.mail.fl_str_mv |
||carlospinto@ccb.ufsc.br |
_version_ |
1799940553070084096 |