Resistência induzida por elicitores e proteção de videira contra míldio
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/5137 |
Resumo: | The grapevine is a fruit that has significant importance in the world economic sector and several factors may compromise its development and especially productivity, including pathogen attack. The disease known as downy mildew is caused by Plasmopara viticola and may cause serious damage to the crop if not controlled. Thus, the major way of controling the disease is associated with the use of fungicides, which causes risks to the environment and human health. The aim of this study was to evaluate the potential of biological agents and chitosan on controlling downy mildew throughthe induced resistance mechanism in grapevine cv. Cabernet Sauvignon. It was hypothesized that the biological agents Trichoderma spp. and Bacillus subtilis, as well as chitosan have the ability to affect directly the viability of P. viticola sporangia and to elicit defense responses in leaf cells of grapevine. Germination of sporangia was measured in vitro andexperiments in greenhouse were conducted, aimed at monitoring the progress of downy mildew on plants exposed to the products studied and, two days later, inoculated with P. viticola. In another experiment, in the absence of the pathogen, leaf tissue of grapevine plants exposed to the same products was collected for conducting biochemical and biological analysis, aiming to characterize the induction of resistance (IR) in plant cells. The activity of pathogenesis related proteins (PRPs) such as guaicol peroxidases, chitinases , β-1,3-glucanases , and polyphenoloxidases in leaves exposed to the products was determined, as well as the levels of total soluble phenols and lignin. Chitosan, but not the biological control agents, reduced the viability of sporangia of P. viticola. All products promoted protection, although not significant when compared to control plants. All studied products produced alterations in the production of PRPs and total phenols. Despite the sharp increase, but not significant, in the lignin content of leaves, small reduction in the area under the mildew progress curve was observed that can be attributed to mechanisms of resistance induction activated by the products studied through elicitor action. |
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2017-05-052017-05-052015-02-27FINGER, Geísa. Resistance induced by elicitors and protection of grapevine against downy mildew. 2015. 75 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2015.http://repositorio.ufsm.br/handle/1/5137The grapevine is a fruit that has significant importance in the world economic sector and several factors may compromise its development and especially productivity, including pathogen attack. The disease known as downy mildew is caused by Plasmopara viticola and may cause serious damage to the crop if not controlled. Thus, the major way of controling the disease is associated with the use of fungicides, which causes risks to the environment and human health. The aim of this study was to evaluate the potential of biological agents and chitosan on controlling downy mildew throughthe induced resistance mechanism in grapevine cv. Cabernet Sauvignon. It was hypothesized that the biological agents Trichoderma spp. and Bacillus subtilis, as well as chitosan have the ability to affect directly the viability of P. viticola sporangia and to elicit defense responses in leaf cells of grapevine. Germination of sporangia was measured in vitro andexperiments in greenhouse were conducted, aimed at monitoring the progress of downy mildew on plants exposed to the products studied and, two days later, inoculated with P. viticola. In another experiment, in the absence of the pathogen, leaf tissue of grapevine plants exposed to the same products was collected for conducting biochemical and biological analysis, aiming to characterize the induction of resistance (IR) in plant cells. The activity of pathogenesis related proteins (PRPs) such as guaicol peroxidases, chitinases , β-1,3-glucanases , and polyphenoloxidases in leaves exposed to the products was determined, as well as the levels of total soluble phenols and lignin. Chitosan, but not the biological control agents, reduced the viability of sporangia of P. viticola. All products promoted protection, although not significant when compared to control plants. All studied products produced alterations in the production of PRPs and total phenols. Despite the sharp increase, but not significant, in the lignin content of leaves, small reduction in the area under the mildew progress curve was observed that can be attributed to mechanisms of resistance induction activated by the products studied through elicitor action.A videira é uma frutífera que possui relevante importância no setor econômico mundial, sendo que vários fatores podem comprometer seu desenvolvimento e consequente rendimento, incluindo o ataque de patógenos. A doença conhecida como míldio da videira, causada pelo pseudofungo Plasmopara viticola, causa sérios danos à cultura quando não controlada. Dessa maneira, a principal forma de controle da doença está associada à utilização de químicos, os quais causam riscos ao ambiente e à saúde do homem. O objetivo deste trabalho foi avaliar o potencial de agentes biológicos e quitosana, na viabilidade de esporângios de míldio, o efeito no progresso da doença e os mecanismos de indução de resistência vegetal, em plantas de videira cv. Cabernet Sauvignon. Hipotetizou-se que os agentes biológicos Trichoderma spp. e Bacillus subtilis, bem como quitosana têm a capacidade de afetar diretamente a viabilidade de esporângios de P. viticola, assim como atrasar ou evitar o progresso de míldio e elicitar respostas de defesa em células foliares de videira. Para tanto, a viabilidade de esporângios de P. viticola foi analisada in vitro e experimentos em casa de vegetação foram conduzidos, afim de, acompanhar o progresso de míldio em plantas de videira expostas aos produtos estudados. Em outro experimento, na ausência do patógeno, tecido foliar de plantas de videira, submetidas aos mesmos produtos, foi coletado para realização de análises bioquímicas e metabólicas, visando à caracterização da indução de resistência (IR) celular vegetal. Foram determinadas atividades de proteínas relacionadas à patogênese (PRPs) como peroxidases de guaicol, quitinases, β-1,3-glucanases e polifenoloxidases em folhas expostas aos produtos, bem como os teores de fenóis solúveis totais e lignina. A quitosana, mas não os agentes biológicos, reduziram a viabilidade dos esporângios. Todos os produtos estudados geraram alterações na produção de PRPs e fenóis totais. Os teores de lignina embora não significativos em relação à testemunha demonstraram aumento, e isso pode estar associado com a porcentagem de proteção que os produtos conferiram às plantas de videira na área abaixo da curva de progresso da doença, induzindo a produção de compostos envolvidos na síntese de lignina.application/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em AgronomiaUFSMBRAgronomiaVitis viniferaBacillus subtilisTrichoderma spp.QuitosanaPR-proteínasLigninaVitis viniferaBacillus subtilisTrichoderma sppChitosan PR-proteinsLigninCNPQ::CIENCIAS AGRARIAS::AGRONOMIAResistência induzida por elicitores e proteção de videira contra míldioResistance induced by elicitors and protection of grapevine against downy mildewinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisBlume, Elenahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4789742J3Muniz, Marlove Fátima Briãohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785175A9Almança, Marcus André Kurtzhttp://lattes.cnpq.br/0577229981274263http://lattes.cnpq.br/1200870656349656Finger, Geísa500100000009400500300500500dd9a87c1-c361-458f-b4bd-b78407907de829cbfa3f-4ae8-4ff3-bc4d-54cadbb9aa9825b7c86e-9d34-4587-b91f-5a6b2c4438437e2c6699-671d-427d-a4f3-2b19e53835dcinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALFINGER, GEISA.pdfapplication/pdf869732http://repositorio.ufsm.br/bitstream/1/5137/1/FINGER%2c%20GEISA.pdf6f19102b6f28184b954a39bcdf2c3f05MD51TEXTFINGER, GEISA.pdf.txtFINGER, GEISA.pdf.txtExtracted texttext/plain149644http://repositorio.ufsm.br/bitstream/1/5137/2/FINGER%2c%20GEISA.pdf.txtb0b1401cc7955fdbf087202c525f5551MD52THUMBNAILFINGER, GEISA.pdf.jpgFINGER, GEISA.pdf.jpgIM Thumbnailimage/jpeg4739http://repositorio.ufsm.br/bitstream/1/5137/3/FINGER%2c%20GEISA.pdf.jpg8425a6077787944a55595b91b508c711MD531/51372017-07-25 11:13:23.547oai:repositorio.ufsm.br:1/5137Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestouvidoria@ufsm.bropendoar:39132017-07-25T14:13:23Repositório Institucional Manancial UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Resistência induzida por elicitores e proteção de videira contra míldio |
dc.title.alternative.eng.fl_str_mv |
Resistance induced by elicitors and protection of grapevine against downy mildew |
title |
Resistência induzida por elicitores e proteção de videira contra míldio |
spellingShingle |
Resistência induzida por elicitores e proteção de videira contra míldio Finger, Geísa Vitis vinifera Bacillus subtilis Trichoderma spp. Quitosana PR-proteínas Lignina Vitis vinifera Bacillus subtilis Trichoderma spp Chitosan PR-proteins Lignin CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Resistência induzida por elicitores e proteção de videira contra míldio |
title_full |
Resistência induzida por elicitores e proteção de videira contra míldio |
title_fullStr |
Resistência induzida por elicitores e proteção de videira contra míldio |
title_full_unstemmed |
Resistência induzida por elicitores e proteção de videira contra míldio |
title_sort |
Resistência induzida por elicitores e proteção de videira contra míldio |
author |
Finger, Geísa |
author_facet |
Finger, Geísa |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Blume, Elena |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4789742J3 |
dc.contributor.referee1.fl_str_mv |
Muniz, Marlove Fátima Brião |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785175A9 |
dc.contributor.referee2.fl_str_mv |
Almança, Marcus André Kurtz |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/0577229981274263 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1200870656349656 |
dc.contributor.author.fl_str_mv |
Finger, Geísa |
contributor_str_mv |
Blume, Elena Muniz, Marlove Fátima Brião Almança, Marcus André Kurtz |
dc.subject.por.fl_str_mv |
Vitis vinifera Bacillus subtilis Trichoderma spp. Quitosana PR-proteínas Lignina |
topic |
Vitis vinifera Bacillus subtilis Trichoderma spp. Quitosana PR-proteínas Lignina Vitis vinifera Bacillus subtilis Trichoderma spp Chitosan PR-proteins Lignin CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
dc.subject.eng.fl_str_mv |
Vitis vinifera Bacillus subtilis Trichoderma spp Chitosan PR-proteins Lignin |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
The grapevine is a fruit that has significant importance in the world economic sector and several factors may compromise its development and especially productivity, including pathogen attack. The disease known as downy mildew is caused by Plasmopara viticola and may cause serious damage to the crop if not controlled. Thus, the major way of controling the disease is associated with the use of fungicides, which causes risks to the environment and human health. The aim of this study was to evaluate the potential of biological agents and chitosan on controlling downy mildew throughthe induced resistance mechanism in grapevine cv. Cabernet Sauvignon. It was hypothesized that the biological agents Trichoderma spp. and Bacillus subtilis, as well as chitosan have the ability to affect directly the viability of P. viticola sporangia and to elicit defense responses in leaf cells of grapevine. Germination of sporangia was measured in vitro andexperiments in greenhouse were conducted, aimed at monitoring the progress of downy mildew on plants exposed to the products studied and, two days later, inoculated with P. viticola. In another experiment, in the absence of the pathogen, leaf tissue of grapevine plants exposed to the same products was collected for conducting biochemical and biological analysis, aiming to characterize the induction of resistance (IR) in plant cells. The activity of pathogenesis related proteins (PRPs) such as guaicol peroxidases, chitinases , β-1,3-glucanases , and polyphenoloxidases in leaves exposed to the products was determined, as well as the levels of total soluble phenols and lignin. Chitosan, but not the biological control agents, reduced the viability of sporangia of P. viticola. All products promoted protection, although not significant when compared to control plants. All studied products produced alterations in the production of PRPs and total phenols. Despite the sharp increase, but not significant, in the lignin content of leaves, small reduction in the area under the mildew progress curve was observed that can be attributed to mechanisms of resistance induction activated by the products studied through elicitor action. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-02-27 |
dc.date.accessioned.fl_str_mv |
2017-05-05 |
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2017-05-05 |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
FINGER, Geísa. Resistance induced by elicitors and protection of grapevine against downy mildew. 2015. 75 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2015. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/5137 |
identifier_str_mv |
FINGER, Geísa. Resistance induced by elicitors and protection of grapevine against downy mildew. 2015. 75 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2015. |
url |
http://repositorio.ufsm.br/handle/1/5137 |
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