Efeito do exercício agudo de curta duração na atividade da enzima δ-aminolevulinato desidratase em humanos
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/11103 |
Resumo: | Aerobic exercise of sufficient intensity and duration can result in increased generation of reactive oxygen species and exercise of extreme endurance may cause oxidative stress with a concomitant decreased activity of antioxidant defense systems. The aim of this study was to investigate the possible effect of a peak of oxidative stress exposition on the activity of blood d-ALA-D, an enzyme sensitive to pro-oxidant situations. The protocol of exercise (treadmill) was divided in rest, submaximal exercise, maximal exercise, and recovery. Oxidative stress biomarkers (TBARS production and δ-ALA-D activity), antioxidant defenses systems (catalase activity, -SH and ascorbic acid) were measured in human blood. The maximal exercise induced an increase in TBARS production and -SH levels during submaximal exercise, maximal exercise and recovery when compared with resting. δ-ALA-D activity increased at maximal exercise and recovery when compared with resting. Catalase activity increased during submaximal exercise and recovery when compared to the rest period. The results described here suggest that d-ALA-D was modulated in a way similar to that observed for other biomarkers of oxidative stress. Complementary investigations analyzing the functional role of d-ALA-D activity need to be performed. Additionally, the results suggest that during the test stages the stimulation of antioxidant defense systems (observed by the increase in thiol group levels) were not sufficient to prevent lipid peroxidation even in trained individuals. |
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2017-04-262017-04-262009-02-27SCHETTERT, Sally Danuta. Effect of the acute exercise of short duration in the enzyme δ-aminolevulinato dehydratase activity in Human. 2009. 71 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2009.http://repositorio.ufsm.br/handle/1/11103Aerobic exercise of sufficient intensity and duration can result in increased generation of reactive oxygen species and exercise of extreme endurance may cause oxidative stress with a concomitant decreased activity of antioxidant defense systems. The aim of this study was to investigate the possible effect of a peak of oxidative stress exposition on the activity of blood d-ALA-D, an enzyme sensitive to pro-oxidant situations. The protocol of exercise (treadmill) was divided in rest, submaximal exercise, maximal exercise, and recovery. Oxidative stress biomarkers (TBARS production and δ-ALA-D activity), antioxidant defenses systems (catalase activity, -SH and ascorbic acid) were measured in human blood. The maximal exercise induced an increase in TBARS production and -SH levels during submaximal exercise, maximal exercise and recovery when compared with resting. δ-ALA-D activity increased at maximal exercise and recovery when compared with resting. Catalase activity increased during submaximal exercise and recovery when compared to the rest period. The results described here suggest that d-ALA-D was modulated in a way similar to that observed for other biomarkers of oxidative stress. Complementary investigations analyzing the functional role of d-ALA-D activity need to be performed. Additionally, the results suggest that during the test stages the stimulation of antioxidant defense systems (observed by the increase in thiol group levels) were not sufficient to prevent lipid peroxidation even in trained individuals.O exercício aeróbico realizado em determinada intensidade e duração pode resultar no aumento da geração de espécies reativas de oxigênio (EROs) sendo que o exercício de extrema resistência pode causar estresse oxidativo com a concomitante diminuição na atividade do sistema de defesa antioxidante. O objetivo deste estudo foi avaliar o efeito na atividade da enzima δ-ALA-D e de outros biomarcadores de estresse oxidativo e defesas antioxidantes em corredores de longa distância submetidos a exercício agudo de curta duração. O protocolo de exercício (realizado em uma esteira ergométrica) foi dividido em repouso, submáximo, máximo e recuperação. Foram avaliados, em sangue humano, os biomarcadores de estresse oxidativo (produção de TBARS e atividade da d-ALAD) sistema de defesa antioxidante (atividade da catalase, -SH e ácido ascórbico). Como resultados, o exercício máximo induziu o aumento na produção de TBARS e níveis de -SH durante o exercício submáximo, máximo e recuperação quando comparados ao repouso. A atividade da d-ALA-D aumentou no exercício máximo e recuperação quando comparada ao período de repouso. Quando comparado ao período de repouso a atividade da catalase aumentou no exercício submáximo e na recuperação. Os resultados deste estudo sugerem que a enzima d-ALAD foi modulada em uma via similar as observadas para outros biomarcadores de estresse oxidativo. Investigações complementares analisando a papel funcional da atividade da d-ALAD necessitam ser realizadas. Ainda, os resultados deste estudo sugerem que durante o protocolo de exercício a estimulação do sistema de defesa antioxidante (observada pelo aumento nos níveis de grupos tióis) não foi suficiente para prevenir a peroxidação lipídica mesmo nos indivíduos treinados.application/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBRBioquímicaEstresse oxidativoExercício físicoδ-aminolevulinato desidrataseOxidative stressPhysical exerciseDelta-aminolevulinate dehydrataseCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAEfeito do exercício agudo de curta duração na atividade da enzima δ-aminolevulinato desidratase em humanosEffect of the acute exercise of short duration in the enzyme δ-aminolevulinato dehydratase activity in Humaninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisCruz, Ivana Beatrice Mânica dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4790333D6Baldisserotto, Bernardohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787286P0Royes, Luiz Fernando Freirehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705849Y0http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4598441Y9Schettert, Sally Danuta2008000000024005003005005000b5fcfe0-b017-4163-80e2-17c110fbcf01213a65cc-eb9d-46da-8a17-48cf03db07316e99a91f-3e85-4198-94b8-37c1e9df1b1b3844a51f-1f7f-46ca-93b7-18d1a9c07d2binfo:eu-repo/semantics/openAccessreponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALSCHETTERT, SALLY DANUTA.pdfapplication/pdf462740http://repositorio.ufsm.br/bitstream/1/11103/1/SCHETTERT%2c%20SALLY%20DANUTA.pdf1499318cd68fdcdee776080b352d8bd0MD51TEXTSCHETTERT, SALLY DANUTA.pdf.txtSCHETTERT, SALLY DANUTA.pdf.txtExtracted texttext/plain114057http://repositorio.ufsm.br/bitstream/1/11103/2/SCHETTERT%2c%20SALLY%20DANUTA.pdf.txtfa04a79116df30e37298fe4830b170a9MD52THUMBNAILSCHETTERT, SALLY DANUTA.pdf.jpgSCHETTERT, SALLY DANUTA.pdf.jpgIM Thumbnailimage/jpeg5634http://repositorio.ufsm.br/bitstream/1/11103/3/SCHETTERT%2c%20SALLY%20DANUTA.pdf.jpg5d4513482ec34dd49b1e33dde30d6e1aMD531/111032017-07-30 01:24:55.32oai:repositorio.ufsm.br:1/11103Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestouvidoria@ufsm.bropendoar:39132017-07-30T04:24:55Repositório Institucional Manancial UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Efeito do exercício agudo de curta duração na atividade da enzima δ-aminolevulinato desidratase em humanos |
dc.title.alternative.eng.fl_str_mv |
Effect of the acute exercise of short duration in the enzyme δ-aminolevulinato dehydratase activity in Human |
title |
Efeito do exercício agudo de curta duração na atividade da enzima δ-aminolevulinato desidratase em humanos |
spellingShingle |
Efeito do exercício agudo de curta duração na atividade da enzima δ-aminolevulinato desidratase em humanos Schettert, Sally Danuta Estresse oxidativo Exercício físico δ-aminolevulinato desidratase Oxidative stress Physical exercise Delta-aminolevulinate dehydratase CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Efeito do exercício agudo de curta duração na atividade da enzima δ-aminolevulinato desidratase em humanos |
title_full |
Efeito do exercício agudo de curta duração na atividade da enzima δ-aminolevulinato desidratase em humanos |
title_fullStr |
Efeito do exercício agudo de curta duração na atividade da enzima δ-aminolevulinato desidratase em humanos |
title_full_unstemmed |
Efeito do exercício agudo de curta duração na atividade da enzima δ-aminolevulinato desidratase em humanos |
title_sort |
Efeito do exercício agudo de curta duração na atividade da enzima δ-aminolevulinato desidratase em humanos |
author |
Schettert, Sally Danuta |
author_facet |
Schettert, Sally Danuta |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Cruz, Ivana Beatrice Mânica da |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4790333D6 |
dc.contributor.referee1.fl_str_mv |
Baldisserotto, Bernardo |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787286P0 |
dc.contributor.referee2.fl_str_mv |
Royes, Luiz Fernando Freire |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705849Y0 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4598441Y9 |
dc.contributor.author.fl_str_mv |
Schettert, Sally Danuta |
contributor_str_mv |
Cruz, Ivana Beatrice Mânica da Baldisserotto, Bernardo Royes, Luiz Fernando Freire |
dc.subject.por.fl_str_mv |
Estresse oxidativo Exercício físico δ-aminolevulinato desidratase |
topic |
Estresse oxidativo Exercício físico δ-aminolevulinato desidratase Oxidative stress Physical exercise Delta-aminolevulinate dehydratase CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Oxidative stress Physical exercise Delta-aminolevulinate dehydratase |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Aerobic exercise of sufficient intensity and duration can result in increased generation of reactive oxygen species and exercise of extreme endurance may cause oxidative stress with a concomitant decreased activity of antioxidant defense systems. The aim of this study was to investigate the possible effect of a peak of oxidative stress exposition on the activity of blood d-ALA-D, an enzyme sensitive to pro-oxidant situations. The protocol of exercise (treadmill) was divided in rest, submaximal exercise, maximal exercise, and recovery. Oxidative stress biomarkers (TBARS production and δ-ALA-D activity), antioxidant defenses systems (catalase activity, -SH and ascorbic acid) were measured in human blood. The maximal exercise induced an increase in TBARS production and -SH levels during submaximal exercise, maximal exercise and recovery when compared with resting. δ-ALA-D activity increased at maximal exercise and recovery when compared with resting. Catalase activity increased during submaximal exercise and recovery when compared to the rest period. The results described here suggest that d-ALA-D was modulated in a way similar to that observed for other biomarkers of oxidative stress. Complementary investigations analyzing the functional role of d-ALA-D activity need to be performed. Additionally, the results suggest that during the test stages the stimulation of antioxidant defense systems (observed by the increase in thiol group levels) were not sufficient to prevent lipid peroxidation even in trained individuals. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009-02-27 |
dc.date.accessioned.fl_str_mv |
2017-04-26 |
dc.date.available.fl_str_mv |
2017-04-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
SCHETTERT, Sally Danuta. Effect of the acute exercise of short duration in the enzyme δ-aminolevulinato dehydratase activity in Human. 2009. 71 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2009. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/11103 |
identifier_str_mv |
SCHETTERT, Sally Danuta. Effect of the acute exercise of short duration in the enzyme δ-aminolevulinato dehydratase activity in Human. 2009. 71 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2009. |
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http://repositorio.ufsm.br/handle/1/11103 |
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Universidade Federal de Santa Maria |
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