Efeito antioxidante de uma nova classe de compostos teluroacetilenos: estudos in vitro e in vivo
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/11130 |
Resumo: | Organotellurium compounds have been reported as antioxidants in several models of oxidative stress, especially in the brain. This study investigated the effect of telluroacetylenes a-d on pharmacological assays in vitro. A second objective of this study was to investigate the antioxidant action of compound b against the oxidative damage induced by sodium nitroprusside (SNP) in mouse brain. In in vitro experiments, lipid peroxidation (LP) and protein carbonyl (PC) levels and δ-aminolevulinate dehydratase (δ-ALA-D) activity were carried out in rat brain homogenate. The mechanisms involved in the antioxidant effect of telluroacetylenes a-d were studied. The glutathione peroxidase (GPx)-like activity, glutathione S-transferase (GST)-like activity and the protection against Fe2+ autooxidation were determined. Furthermore, the scavenger effect of 2,2 -diphenyl-1-picrylhydrazyl (DPPH ) and 2,2 -azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS +) radicals and hydrogen peroxide (H2O2) were investigated. In in vivo experiments, mice received SNP (0.335 μmol per site) intra cerebroventricular (i.c.v.) thirty minutes after oral administration of telluroacetylene b (10 mg/kg). After 1 h, animals were euthanized. The levels of LP and δ- ALA-D, GPx, GST, catalase (CAT) and glutathione reductase (GR) activities were carried out in mouse brain homogenate. Telluroacetylenes a-d, at low μM range, reduced LP and PC levels in rat brain homogenate. Telluroacetylenes a-d showed effect of scavenging DPPH and ABTS + radicals and H2O2. However the compounds had no GPx-like and GST-like activities or protected against Fe2+ autooxidation, discarding that these mechanisms are involved in the antioxidant effect of telluroacetylenes a-d. δ-ALA-D activity was inhibited by telluroacetylenes a-d, at high μM range, in rat brain homogenate. In the in vivo experiments, brains of mice treated with SNP showed an increase in LP and the reduction in δ-ALA-D, GR and GST activities. Telluroacetylene b protected against the oxidative stress caused by SNP in brain of mice. Moreover, telluroacetylene b incresead per se GPx activity in brains of mice. The results support an antioxidant effect of telluroacetylenes a-d in vitro. Telluroacetylene b protected against oxidative damage caused by SNP in mouse brain, suggesting an antioxidant effect of this compound in vivo. |
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2010-08-232010-08-232010-04-01SOUZA, Ana Cristina Guerra de. Antioxidant effect of a novel class of telluroacetylene compounds: studies in vitro and in vivo. 2010. 60 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2010.http://repositorio.ufsm.br/handle/1/11130Organotellurium compounds have been reported as antioxidants in several models of oxidative stress, especially in the brain. This study investigated the effect of telluroacetylenes a-d on pharmacological assays in vitro. A second objective of this study was to investigate the antioxidant action of compound b against the oxidative damage induced by sodium nitroprusside (SNP) in mouse brain. In in vitro experiments, lipid peroxidation (LP) and protein carbonyl (PC) levels and δ-aminolevulinate dehydratase (δ-ALA-D) activity were carried out in rat brain homogenate. The mechanisms involved in the antioxidant effect of telluroacetylenes a-d were studied. The glutathione peroxidase (GPx)-like activity, glutathione S-transferase (GST)-like activity and the protection against Fe2+ autooxidation were determined. Furthermore, the scavenger effect of 2,2 -diphenyl-1-picrylhydrazyl (DPPH ) and 2,2 -azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS +) radicals and hydrogen peroxide (H2O2) were investigated. In in vivo experiments, mice received SNP (0.335 μmol per site) intra cerebroventricular (i.c.v.) thirty minutes after oral administration of telluroacetylene b (10 mg/kg). After 1 h, animals were euthanized. The levels of LP and δ- ALA-D, GPx, GST, catalase (CAT) and glutathione reductase (GR) activities were carried out in mouse brain homogenate. Telluroacetylenes a-d, at low μM range, reduced LP and PC levels in rat brain homogenate. Telluroacetylenes a-d showed effect of scavenging DPPH and ABTS + radicals and H2O2. However the compounds had no GPx-like and GST-like activities or protected against Fe2+ autooxidation, discarding that these mechanisms are involved in the antioxidant effect of telluroacetylenes a-d. δ-ALA-D activity was inhibited by telluroacetylenes a-d, at high μM range, in rat brain homogenate. In the in vivo experiments, brains of mice treated with SNP showed an increase in LP and the reduction in δ-ALA-D, GR and GST activities. Telluroacetylene b protected against the oxidative stress caused by SNP in brain of mice. Moreover, telluroacetylene b incresead per se GPx activity in brains of mice. The results support an antioxidant effect of telluroacetylenes a-d in vitro. Telluroacetylene b protected against oxidative damage caused by SNP in mouse brain, suggesting an antioxidant effect of this compound in vivo.Compostos orgânicos de telúrio apresentam propriedades antioxidantes em muitos modelos de estresse oxidativo, principalmente no cérebro. Este estudo investigou o efeito de compostos teluroacetilenos a-d em testes farmacológicos in vitro. Um segundo objetivo deste trabalho foi investigar a ação antioxidante do composto b contra o dano oxidativo induzido por nitroprussiato de sódio (SNP) em cérebro de camundongos. Nos experimentos in vitro, os níveis de peroxidação lipídica (PL) e proteína carbonilada (PC) e a atividade da δ-aminolevulinato desidratase (δ-ALA-D) foram determinados em homogeneizado de cérebro de rato. Para estudar o mecanismo pelo qual os teluroacetilenos apresentaram efeito antioxidante, verificou-se o efeito mimético destes compostos na atividade das enzimas glutationa peroxidase (GPx) e glutationa S-transferase (GST) e o efeito protetor na auto-oxidação do Fe2+. Além disso, estudou-se o efeito dos teluroacetilenos a-d como scavenger dos radicais 2,2 -difenil-1-picril-hidrazil (DPPH ) e 2,2 -azino-bis(3-etilbenzotiazolina-6-ácido sulfônico) (ABTS +) e o efeito scavenger de peróxido de hidrogênio (H2O2). Nos experimentos in vivo, camundongos receberam SNP (0,335 μmol per site) intracerebroventricular (i.c.v.) trinta minutos após a administração oral de teluroacetileno b (10 mg/kg). Após uma hora, os animais foram submetidos à eutanásia e foram determinados os níveis de PL e as atividades das enzimas δ-ALA-D, GPx, GST, catalase (CAT) e glutationa redutase (GR) no cérebro de camundongos. Os teluroacetilenos a-d, em baixas concentrações, reduziram os níveis de PL e PC no homogeneizado de cérebro de rato. Os teluroacetilenos a-d apresentaram efeito scavenger de radicais DPPH e ABTS +, bem como efeito scavenger de H2O2. Entretanto, os compostos não apresentaram efeito mimético da atividade das enzimas GPx e GST, nem efeito protetor contra a auto-oxidação do Fe2+, descartando que estes mecanismos estariam envolvidos na ação antioxidante dos teluroacetilenos a-d. A atividade da δ-ALA-D foi inibida pelos teluroacetilenos a-d em homogeneizado de cérebro de ratos somente em concentrações maiores do que as necessárias para o efeito antioxidante. Nos experimentos in vivo, os cérebros dos camundongos tratados com SNP apresentaram um aumento nos níveis de PL e inibição na atividade das enzimas δ-ALA-D, GR e GST. O teluroacetileno b protegeu contra o estresse oxidativo causado pelo SNP em cérebro de camundongos. Ainda, o teluroacetileno b aumentou per se a atividade da GPx em cérebro de camundongos. Estes resultados comprovam o efeito antioxidante dos teluroacetilenos a-d in vitro. Além disso, o teluroacetileno b protegeu contra o dano oxidativo causado pelo SNP em cérebro de camundongos, demonstrando o efeito antioxidante deste composto in vivo.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBRBioquímicaTelúrioPeroxidação lipídicaAntioxidanteCérebroNitroprussiato de sódioTelluriumLipid peroxidationAntioxidantBrainSodium nitroprussideCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAEfeito antioxidante de uma nova classe de compostos teluroacetilenos: estudos in vitro e in vivoAntioxidant effect of a novel class of telluroacetylene compounds: studies in vitro and in vivoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisNogueira, Cristina Waynehttp://lattes.cnpq.br/2877042401245169Fachinetto, Roseleihttp://lattes.cnpq.br/7203076675431306Furian, Ana Fláviahttp://lattes.cnpq.br/0865191340133424http://lattes.cnpq.br/7847265932318162Souza, Ana Cristina Guerra de2008000000024005005005005000186436d-f662-4a5e-b36e-8c8ab8e10bf87bcdc2bf-b6e8-4045-9845-3114b0fe6d93d4a64262-0e7e-45c5-910e-0b0aee907c10ccd57a08-6ff7-4098-88ec-5cb936e92f93info:eu-repo/semantics/openAccessreponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALSOUZA, ANA CRISTINA GUERRA DE.pdfapplication/pdf2848085http://repositorio.ufsm.br/bitstream/1/11130/1/SOUZA%2c%20ANA%20CRISTINA%20GUERRA%20DE.pdf55b69f74ce1a6457179672fe42fc0cffMD51TEXTSOUZA, ANA CRISTINA GUERRA DE.pdf.txtSOUZA, ANA CRISTINA GUERRA DE.pdf.txtExtracted texttext/plain98838http://repositorio.ufsm.br/bitstream/1/11130/2/SOUZA%2c%20ANA%20CRISTINA%20GUERRA%20DE.pdf.txt667dabc446cc9364adcf98faf2ce3b98MD52THUMBNAILSOUZA, ANA CRISTINA GUERRA DE.pdf.jpgSOUZA, ANA CRISTINA GUERRA DE.pdf.jpgIM Thumbnailimage/jpeg4893http://repositorio.ufsm.br/bitstream/1/11130/3/SOUZA%2c%20ANA%20CRISTINA%20GUERRA%20DE.pdf.jpge7870b3f5bc9399e32eb27c05f3e5f9bMD531/111302022-01-07 16:08:09.171oai:repositorio.ufsm.br:1/11130Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestouvidoria@ufsm.bropendoar:39132022-01-07T19:08:09Repositório Institucional Manancial UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Efeito antioxidante de uma nova classe de compostos teluroacetilenos: estudos in vitro e in vivo |
dc.title.alternative.eng.fl_str_mv |
Antioxidant effect of a novel class of telluroacetylene compounds: studies in vitro and in vivo |
title |
Efeito antioxidante de uma nova classe de compostos teluroacetilenos: estudos in vitro e in vivo |
spellingShingle |
Efeito antioxidante de uma nova classe de compostos teluroacetilenos: estudos in vitro e in vivo Souza, Ana Cristina Guerra de Telúrio Peroxidação lipídica Antioxidante Cérebro Nitroprussiato de sódio Tellurium Lipid peroxidation Antioxidant Brain Sodium nitroprusside CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Efeito antioxidante de uma nova classe de compostos teluroacetilenos: estudos in vitro e in vivo |
title_full |
Efeito antioxidante de uma nova classe de compostos teluroacetilenos: estudos in vitro e in vivo |
title_fullStr |
Efeito antioxidante de uma nova classe de compostos teluroacetilenos: estudos in vitro e in vivo |
title_full_unstemmed |
Efeito antioxidante de uma nova classe de compostos teluroacetilenos: estudos in vitro e in vivo |
title_sort |
Efeito antioxidante de uma nova classe de compostos teluroacetilenos: estudos in vitro e in vivo |
author |
Souza, Ana Cristina Guerra de |
author_facet |
Souza, Ana Cristina Guerra de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Nogueira, Cristina Wayne |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2877042401245169 |
dc.contributor.referee1.fl_str_mv |
Fachinetto, Roselei |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/7203076675431306 |
dc.contributor.referee2.fl_str_mv |
Furian, Ana Flávia |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/0865191340133424 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7847265932318162 |
dc.contributor.author.fl_str_mv |
Souza, Ana Cristina Guerra de |
contributor_str_mv |
Nogueira, Cristina Wayne Fachinetto, Roselei Furian, Ana Flávia |
dc.subject.por.fl_str_mv |
Telúrio Peroxidação lipídica Antioxidante Cérebro Nitroprussiato de sódio |
topic |
Telúrio Peroxidação lipídica Antioxidante Cérebro Nitroprussiato de sódio Tellurium Lipid peroxidation Antioxidant Brain Sodium nitroprusside CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Tellurium Lipid peroxidation Antioxidant Brain Sodium nitroprusside |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Organotellurium compounds have been reported as antioxidants in several models of oxidative stress, especially in the brain. This study investigated the effect of telluroacetylenes a-d on pharmacological assays in vitro. A second objective of this study was to investigate the antioxidant action of compound b against the oxidative damage induced by sodium nitroprusside (SNP) in mouse brain. In in vitro experiments, lipid peroxidation (LP) and protein carbonyl (PC) levels and δ-aminolevulinate dehydratase (δ-ALA-D) activity were carried out in rat brain homogenate. The mechanisms involved in the antioxidant effect of telluroacetylenes a-d were studied. The glutathione peroxidase (GPx)-like activity, glutathione S-transferase (GST)-like activity and the protection against Fe2+ autooxidation were determined. Furthermore, the scavenger effect of 2,2 -diphenyl-1-picrylhydrazyl (DPPH ) and 2,2 -azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS +) radicals and hydrogen peroxide (H2O2) were investigated. In in vivo experiments, mice received SNP (0.335 μmol per site) intra cerebroventricular (i.c.v.) thirty minutes after oral administration of telluroacetylene b (10 mg/kg). After 1 h, animals were euthanized. The levels of LP and δ- ALA-D, GPx, GST, catalase (CAT) and glutathione reductase (GR) activities were carried out in mouse brain homogenate. Telluroacetylenes a-d, at low μM range, reduced LP and PC levels in rat brain homogenate. Telluroacetylenes a-d showed effect of scavenging DPPH and ABTS + radicals and H2O2. However the compounds had no GPx-like and GST-like activities or protected against Fe2+ autooxidation, discarding that these mechanisms are involved in the antioxidant effect of telluroacetylenes a-d. δ-ALA-D activity was inhibited by telluroacetylenes a-d, at high μM range, in rat brain homogenate. In the in vivo experiments, brains of mice treated with SNP showed an increase in LP and the reduction in δ-ALA-D, GR and GST activities. Telluroacetylene b protected against the oxidative stress caused by SNP in brain of mice. Moreover, telluroacetylene b incresead per se GPx activity in brains of mice. The results support an antioxidant effect of telluroacetylenes a-d in vitro. Telluroacetylene b protected against oxidative damage caused by SNP in mouse brain, suggesting an antioxidant effect of this compound in vivo. |
publishDate |
2010 |
dc.date.accessioned.fl_str_mv |
2010-08-23 |
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2010-08-23 |
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2010-04-01 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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SOUZA, Ana Cristina Guerra de. Antioxidant effect of a novel class of telluroacetylene compounds: studies in vitro and in vivo. 2010. 60 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/11130 |
identifier_str_mv |
SOUZA, Ana Cristina Guerra de. Antioxidant effect of a novel class of telluroacetylene compounds: studies in vitro and in vivo. 2010. 60 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2010. |
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http://repositorio.ufsm.br/handle/1/11130 |
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Universidade Federal de Santa Maria |
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