Avaliação da atividade biológica in vitro da amitriptilina
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/20377 |
Resumo: | Theemergenceofinfectionscaused bymulti-drug resistantbacteriaworldwideand thesubstantialincreasein cancerhasled tothesearchforinnovativetherapies.Inthisway,theuseofresearcheswithdrugsalreadyinclinicalusethatcould providemoreeffectivetreatmentalternativesto overcomethesecurrentchallenges,dueto thelimited numberofavailabletherapeuticagents,characterizing therepositioning orredirectionofdrugs,wasused.Amitriptylineisatricyclicantidepressantnotwidelyused intheclinicforthispurposebecauseofitsmarked sideeffectsinthedoseat whichithasto beused.However,atalowerdose,itsuseiscurrentlyinthetreatmentofpain,suchasprophylacticmigraineand cancerpain.However,studieshavedemonstrated theiractivityagainstvarioustypesofbacteriaand fungi.Duetoitsdiversified biologicalactivitycoupled withtherelativelylow priceand theisolation ofmultiresistantbacteria(MDR)mainlyatthehospitallevel,wewereencouraged to conductthisresearch.Oftendrugsthat arecapableofcleaving DNAdouble-stranded haveboth antibacterialand anti-cancereffects.Thisknowledgeand theexistenceofonlyhypothesesin theclarificationofthemechanismofactionofthisdrugistheobjectiveofthisstudyinwhich weintend to evaluatetheinvitrobiologicalactivityofthedrugamitriptylineagainstAmerican Standard CultureCollection (ATCC)referencestandard strainsand clinicalisolatesMDR,bydetermining theminimuminhibitoryconcentration(MIC),minimumbacterialconcentration (MBC),tolerancelevel,fractionalinhibitoryconcentrationindex(FICI),associated ornot with antibioticsused inclinicalpracticeand cleavageabilityofthepUC18 doublestranded plasmidDNA. |
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2021-03-04T11:08:13Z2021-03-04T11:08:13Z2019-08-29http://repositorio.ufsm.br/handle/1/20377Theemergenceofinfectionscaused bymulti-drug resistantbacteriaworldwideand thesubstantialincreasein cancerhasled tothesearchforinnovativetherapies.Inthisway,theuseofresearcheswithdrugsalreadyinclinicalusethatcould providemoreeffectivetreatmentalternativesto overcomethesecurrentchallenges,dueto thelimited numberofavailabletherapeuticagents,characterizing therepositioning orredirectionofdrugs,wasused.Amitriptylineisatricyclicantidepressantnotwidelyused intheclinicforthispurposebecauseofitsmarked sideeffectsinthedoseat whichithasto beused.However,atalowerdose,itsuseiscurrentlyinthetreatmentofpain,suchasprophylacticmigraineand cancerpain.However,studieshavedemonstrated theiractivityagainstvarioustypesofbacteriaand fungi.Duetoitsdiversified biologicalactivitycoupled withtherelativelylow priceand theisolation ofmultiresistantbacteria(MDR)mainlyatthehospitallevel,wewereencouraged to conductthisresearch.Oftendrugsthat arecapableofcleaving DNAdouble-stranded haveboth antibacterialand anti-cancereffects.Thisknowledgeand theexistenceofonlyhypothesesin theclarificationofthemechanismofactionofthisdrugistheobjectiveofthisstudyinwhich weintend to evaluatetheinvitrobiologicalactivityofthedrugamitriptylineagainstAmerican Standard CultureCollection (ATCC)referencestandard strainsand clinicalisolatesMDR,bydetermining theminimuminhibitoryconcentration(MIC),minimumbacterialconcentration (MBC),tolerancelevel,fractionalinhibitoryconcentrationindex(FICI),associated ornot with antibioticsused inclinicalpracticeand cleavageabilityofthepUC18 doublestranded plasmidDNA.Aemergênciadeinfecçõescausadasporbactériasresistentesamúltiplasdrogasnomundotodoeo substancialaumentodo câncerconduziu aprocuraporterapiasinovadoras.Dessamaneira,lançou-semãodautilizaçãodepesquisascommedicamentosjáemusoclínicoquepudessemproporcionaralternativasdetratamento maiseficazesparasuperarestesdesafiosatuais,devidoaolimitadonúmerodeagentesterapêuticosdisponíveis,caracterizando-seoreposicionamentoou redirecionamento dedrogas.Aamitriptilinaéumantidepressivotricícliconãomuitoutilizadonaclínicaparaestefim,devidoaseusefeitoscolateraisacentuadosnadoseemquetemqueserutilizada. Porém,emdosemenor,seu empregoatualmenteénotratamentodador,comoporexemplo,profiláticonaenxaquecaedadoroncológica. Contudo,estudosdemostraramsuaatividadefrenteaváriostiposdebactériasefungos.Devidosuadiversificadaatividadebiológicaaliadaaopreço relativamentebaixoeao isolamento debactériasmultirresistentes(MDR)principalmenteanível hospitar,nosincentivouaconduzirestapesquisa.FrequentementemedicamentosaptosaclivaroDNAfitaduplapossuemtantoefeitoantibacterianocomoanticancerígeno.Estesconhecimentoseaexistênciasomentedehipótesesnoesclarecimentodomecanismodeaçãodestadrogaconstituioobjetivodesteestudonoqualpretendemosavaliaraatividadebiológicain vitro do medicamento amitriptilinafrenteacepasbacterianaspadrãodereferênciaAmerican TypeCultureCollection (ATCC)eisoladosclínicosMDR, atravésdadeterminaçãodaconcentraçãoinibitóriamínima(CIM),daconcentração bactericidamínima(CBM),doníveldetolerância,do índicedeconcentração inibitóriafracionada(FICI),associadasounão aantibióticosusadosnaclínicamédicaedacapacidadedeclivagemdoDNAplasmidialfitaduplapUC18.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências da SaúdePrograma de Pós-Graduação em Ciências FarmacêuticasUFSMBrasilFarmacologiaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAmitriptilinaCompostos antibacterianosReposicionamento de drogasClivagem do DNAAmitriptylineAntibacterial compoundsRepositioning drugsDNA cleavageCNPQ::CIENCIAS DA SAUDE::FARMACIAAvaliação da atividade biológica in vitro da amitriptilinaEvaluation of the in vitro biological activity of amitriptilineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisHorner, Rosmarihttp://lattes.cnpq.br/5907084134183708Krause, Luciana Maria Fontanarihttp://lattes.cnpq.br/9844890896121847Montagner, Franciscohttp://lattes.cnpq.br/2937031469516770http://lattes.cnpq.br/4725838792308536Machado, Catrine de Souza4003000000056005fdaffc5-abc5-4607-8952-fc5c4760b0043cec4ce2-e51b-4f55-94fa-6aab34e73f57a310dec0-114a-4a8c-836d-d08756d18299ccf63a8c-bf4f-4d3e-baec-1976c89564b4reponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGCF_2019_MACHADO_CATRINE.pdfDIS_PPGCF_2019_MACHADO_CATRINE.pdfDissertação de Mestradoapplication/pdf2084751http://repositorio.ufsm.br/bitstream/1/20377/1/DIS_PPGCF_2019_MACHADO_CATRINE.pdfd87154502abb5b438e5f3c0bed355457MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Avaliação da atividade biológica in vitro da amitriptilina |
dc.title.alternative.eng.fl_str_mv |
Evaluation of the in vitro biological activity of amitriptiline |
title |
Avaliação da atividade biológica in vitro da amitriptilina |
spellingShingle |
Avaliação da atividade biológica in vitro da amitriptilina Machado, Catrine de Souza Amitriptilina Compostos antibacterianos Reposicionamento de drogas Clivagem do DNA Amitriptyline Antibacterial compounds Repositioning drugs DNA cleavage CNPQ::CIENCIAS DA SAUDE::FARMACIA |
title_short |
Avaliação da atividade biológica in vitro da amitriptilina |
title_full |
Avaliação da atividade biológica in vitro da amitriptilina |
title_fullStr |
Avaliação da atividade biológica in vitro da amitriptilina |
title_full_unstemmed |
Avaliação da atividade biológica in vitro da amitriptilina |
title_sort |
Avaliação da atividade biológica in vitro da amitriptilina |
author |
Machado, Catrine de Souza |
author_facet |
Machado, Catrine de Souza |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Horner, Rosmari |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5907084134183708 |
dc.contributor.referee1.fl_str_mv |
Krause, Luciana Maria Fontanari |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/9844890896121847 |
dc.contributor.referee2.fl_str_mv |
Montagner, Francisco |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/2937031469516770 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4725838792308536 |
dc.contributor.author.fl_str_mv |
Machado, Catrine de Souza |
contributor_str_mv |
Horner, Rosmari Krause, Luciana Maria Fontanari Montagner, Francisco |
dc.subject.por.fl_str_mv |
Amitriptilina Compostos antibacterianos Reposicionamento de drogas Clivagem do DNA |
topic |
Amitriptilina Compostos antibacterianos Reposicionamento de drogas Clivagem do DNA Amitriptyline Antibacterial compounds Repositioning drugs DNA cleavage CNPQ::CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
Amitriptyline Antibacterial compounds Repositioning drugs DNA cleavage |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::FARMACIA |
description |
Theemergenceofinfectionscaused bymulti-drug resistantbacteriaworldwideand thesubstantialincreasein cancerhasled tothesearchforinnovativetherapies.Inthisway,theuseofresearcheswithdrugsalreadyinclinicalusethatcould providemoreeffectivetreatmentalternativesto overcomethesecurrentchallenges,dueto thelimited numberofavailabletherapeuticagents,characterizing therepositioning orredirectionofdrugs,wasused.Amitriptylineisatricyclicantidepressantnotwidelyused intheclinicforthispurposebecauseofitsmarked sideeffectsinthedoseat whichithasto beused.However,atalowerdose,itsuseiscurrentlyinthetreatmentofpain,suchasprophylacticmigraineand cancerpain.However,studieshavedemonstrated theiractivityagainstvarioustypesofbacteriaand fungi.Duetoitsdiversified biologicalactivitycoupled withtherelativelylow priceand theisolation ofmultiresistantbacteria(MDR)mainlyatthehospitallevel,wewereencouraged to conductthisresearch.Oftendrugsthat arecapableofcleaving DNAdouble-stranded haveboth antibacterialand anti-cancereffects.Thisknowledgeand theexistenceofonlyhypothesesin theclarificationofthemechanismofactionofthisdrugistheobjectiveofthisstudyinwhich weintend to evaluatetheinvitrobiologicalactivityofthedrugamitriptylineagainstAmerican Standard CultureCollection (ATCC)referencestandard strainsand clinicalisolatesMDR,bydetermining theminimuminhibitoryconcentration(MIC),minimumbacterialconcentration (MBC),tolerancelevel,fractionalinhibitoryconcentrationindex(FICI),associated ornot with antibioticsused inclinicalpracticeand cleavageabilityofthepUC18 doublestranded plasmidDNA. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019-08-29 |
dc.date.accessioned.fl_str_mv |
2021-03-04T11:08:13Z |
dc.date.available.fl_str_mv |
2021-03-04T11:08:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/20377 |
url |
http://repositorio.ufsm.br/handle/1/20377 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
400300000005 |
dc.relation.confidence.fl_str_mv |
600 |
dc.relation.authority.fl_str_mv |
5fdaffc5-abc5-4607-8952-fc5c4760b004 3cec4ce2-e51b-4f55-94fa-6aab34e73f57 a310dec0-114a-4a8c-836d-d08756d18299 ccf63a8c-bf4f-4d3e-baec-1976c89564b4 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências da Saúde |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Farmacêuticas |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Farmacologia |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências da Saúde |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional Manancial UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
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Universidade Federal de Santa Maria (UFSM) |
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UFSM |
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Repositório Institucional Manancial UFSM |
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