Avaliação da atividade biológica in vitro da amitriptilina

Detalhes bibliográficos
Autor(a) principal: Machado, Catrine de Souza
Data de Publicação: 2019
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional Manancial UFSM
Texto Completo: http://repositorio.ufsm.br/handle/1/20377
Resumo: Theemergenceofinfectionscaused bymulti-drug resistantbacteriaworldwideand thesubstantialincreasein cancerhasled tothesearchforinnovativetherapies.Inthisway,theuseofresearcheswithdrugsalreadyinclinicalusethatcould providemoreeffectivetreatmentalternativesto overcomethesecurrentchallenges,dueto thelimited numberofavailabletherapeuticagents,characterizing therepositioning orredirectionofdrugs,wasused.Amitriptylineisatricyclicantidepressantnotwidelyused intheclinicforthispurposebecauseofitsmarked sideeffectsinthedoseat whichithasto beused.However,atalowerdose,itsuseiscurrentlyinthetreatmentofpain,suchasprophylacticmigraineand cancerpain.However,studieshavedemonstrated theiractivityagainstvarioustypesofbacteriaand fungi.Duetoitsdiversified biologicalactivitycoupled withtherelativelylow priceand theisolation ofmultiresistantbacteria(MDR)mainlyatthehospitallevel,wewereencouraged to conductthisresearch.Oftendrugsthat arecapableofcleaving DNAdouble-stranded haveboth antibacterialand anti-cancereffects.Thisknowledgeand theexistenceofonlyhypothesesin theclarificationofthemechanismofactionofthisdrugistheobjectiveofthisstudyinwhich weintend to evaluatetheinvitrobiologicalactivityofthedrugamitriptylineagainstAmerican Standard CultureCollection (ATCC)referencestandard strainsand clinicalisolatesMDR,bydetermining theminimuminhibitoryconcentration(MIC),minimumbacterialconcentration (MBC),tolerancelevel,fractionalinhibitoryconcentrationindex(FICI),associated ornot with antibioticsused inclinicalpracticeand cleavageabilityofthepUC18 doublestranded plasmidDNA.
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spelling 2021-03-04T11:08:13Z2021-03-04T11:08:13Z2019-08-29http://repositorio.ufsm.br/handle/1/20377Theemergenceofinfectionscaused bymulti-drug resistantbacteriaworldwideand thesubstantialincreasein cancerhasled tothesearchforinnovativetherapies.Inthisway,theuseofresearcheswithdrugsalreadyinclinicalusethatcould providemoreeffectivetreatmentalternativesto overcomethesecurrentchallenges,dueto thelimited numberofavailabletherapeuticagents,characterizing therepositioning orredirectionofdrugs,wasused.Amitriptylineisatricyclicantidepressantnotwidelyused intheclinicforthispurposebecauseofitsmarked sideeffectsinthedoseat whichithasto beused.However,atalowerdose,itsuseiscurrentlyinthetreatmentofpain,suchasprophylacticmigraineand cancerpain.However,studieshavedemonstrated theiractivityagainstvarioustypesofbacteriaand fungi.Duetoitsdiversified biologicalactivitycoupled withtherelativelylow priceand theisolation ofmultiresistantbacteria(MDR)mainlyatthehospitallevel,wewereencouraged to conductthisresearch.Oftendrugsthat arecapableofcleaving DNAdouble-stranded haveboth antibacterialand anti-cancereffects.Thisknowledgeand theexistenceofonlyhypothesesin theclarificationofthemechanismofactionofthisdrugistheobjectiveofthisstudyinwhich weintend to evaluatetheinvitrobiologicalactivityofthedrugamitriptylineagainstAmerican Standard CultureCollection (ATCC)referencestandard strainsand clinicalisolatesMDR,bydetermining theminimuminhibitoryconcentration(MIC),minimumbacterialconcentration (MBC),tolerancelevel,fractionalinhibitoryconcentrationindex(FICI),associated ornot with antibioticsused inclinicalpracticeand cleavageabilityofthepUC18 doublestranded plasmidDNA.Aemergênciadeinfecçõescausadasporbactériasresistentesamúltiplasdrogasnomundotodoeo substancialaumentodo câncerconduziu aprocuraporterapiasinovadoras.Dessamaneira,lançou-semãodautilizaçãodepesquisascommedicamentosjáemusoclínicoquepudessemproporcionaralternativasdetratamento maiseficazesparasuperarestesdesafiosatuais,devidoaolimitadonúmerodeagentesterapêuticosdisponíveis,caracterizando-seoreposicionamentoou redirecionamento dedrogas.Aamitriptilinaéumantidepressivotricícliconãomuitoutilizadonaclínicaparaestefim,devidoaseusefeitoscolateraisacentuadosnadoseemquetemqueserutilizada. Porém,emdosemenor,seu empregoatualmenteénotratamentodador,comoporexemplo,profiláticonaenxaquecaedadoroncológica. Contudo,estudosdemostraramsuaatividadefrenteaváriostiposdebactériasefungos.Devidosuadiversificadaatividadebiológicaaliadaaopreço relativamentebaixoeao isolamento debactériasmultirresistentes(MDR)principalmenteanível hospitar,nosincentivouaconduzirestapesquisa.FrequentementemedicamentosaptosaclivaroDNAfitaduplapossuemtantoefeitoantibacterianocomoanticancerígeno.Estesconhecimentoseaexistênciasomentedehipótesesnoesclarecimentodomecanismodeaçãodestadrogaconstituioobjetivodesteestudonoqualpretendemosavaliaraatividadebiológicain vitro do medicamento amitriptilinafrenteacepasbacterianaspadrãodereferênciaAmerican TypeCultureCollection (ATCC)eisoladosclínicosMDR, atravésdadeterminaçãodaconcentraçãoinibitóriamínima(CIM),daconcentração bactericidamínima(CBM),doníveldetolerância,do índicedeconcentração inibitóriafracionada(FICI),associadasounão aantibióticosusadosnaclínicamédicaedacapacidadedeclivagemdoDNAplasmidialfitaduplapUC18.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências da SaúdePrograma de Pós-Graduação em Ciências FarmacêuticasUFSMBrasilFarmacologiaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAmitriptilinaCompostos antibacterianosReposicionamento de drogasClivagem do DNAAmitriptylineAntibacterial compoundsRepositioning drugsDNA cleavageCNPQ::CIENCIAS DA SAUDE::FARMACIAAvaliação da atividade biológica in vitro da amitriptilinaEvaluation of the in vitro biological activity of amitriptilineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisHorner, Rosmarihttp://lattes.cnpq.br/5907084134183708Krause, Luciana Maria Fontanarihttp://lattes.cnpq.br/9844890896121847Montagner, Franciscohttp://lattes.cnpq.br/2937031469516770http://lattes.cnpq.br/4725838792308536Machado, Catrine de Souza4003000000056005fdaffc5-abc5-4607-8952-fc5c4760b0043cec4ce2-e51b-4f55-94fa-6aab34e73f57a310dec0-114a-4a8c-836d-d08756d18299ccf63a8c-bf4f-4d3e-baec-1976c89564b4reponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGCF_2019_MACHADO_CATRINE.pdfDIS_PPGCF_2019_MACHADO_CATRINE.pdfDissertação de Mestradoapplication/pdf2084751http://repositorio.ufsm.br/bitstream/1/20377/1/DIS_PPGCF_2019_MACHADO_CATRINE.pdfd87154502abb5b438e5f3c0bed355457MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv Avaliação da atividade biológica in vitro da amitriptilina
dc.title.alternative.eng.fl_str_mv Evaluation of the in vitro biological activity of amitriptiline
title Avaliação da atividade biológica in vitro da amitriptilina
spellingShingle Avaliação da atividade biológica in vitro da amitriptilina
Machado, Catrine de Souza
Amitriptilina
Compostos antibacterianos
Reposicionamento de drogas
Clivagem do DNA
Amitriptyline
Antibacterial compounds
Repositioning drugs
DNA cleavage
CNPQ::CIENCIAS DA SAUDE::FARMACIA
title_short Avaliação da atividade biológica in vitro da amitriptilina
title_full Avaliação da atividade biológica in vitro da amitriptilina
title_fullStr Avaliação da atividade biológica in vitro da amitriptilina
title_full_unstemmed Avaliação da atividade biológica in vitro da amitriptilina
title_sort Avaliação da atividade biológica in vitro da amitriptilina
author Machado, Catrine de Souza
author_facet Machado, Catrine de Souza
author_role author
dc.contributor.advisor1.fl_str_mv Horner, Rosmari
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/5907084134183708
dc.contributor.referee1.fl_str_mv Krause, Luciana Maria Fontanari
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/9844890896121847
dc.contributor.referee2.fl_str_mv Montagner, Francisco
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/2937031469516770
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/4725838792308536
dc.contributor.author.fl_str_mv Machado, Catrine de Souza
contributor_str_mv Horner, Rosmari
Krause, Luciana Maria Fontanari
Montagner, Francisco
dc.subject.por.fl_str_mv Amitriptilina
Compostos antibacterianos
Reposicionamento de drogas
Clivagem do DNA
topic Amitriptilina
Compostos antibacterianos
Reposicionamento de drogas
Clivagem do DNA
Amitriptyline
Antibacterial compounds
Repositioning drugs
DNA cleavage
CNPQ::CIENCIAS DA SAUDE::FARMACIA
dc.subject.eng.fl_str_mv Amitriptyline
Antibacterial compounds
Repositioning drugs
DNA cleavage
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS DA SAUDE::FARMACIA
description Theemergenceofinfectionscaused bymulti-drug resistantbacteriaworldwideand thesubstantialincreasein cancerhasled tothesearchforinnovativetherapies.Inthisway,theuseofresearcheswithdrugsalreadyinclinicalusethatcould providemoreeffectivetreatmentalternativesto overcomethesecurrentchallenges,dueto thelimited numberofavailabletherapeuticagents,characterizing therepositioning orredirectionofdrugs,wasused.Amitriptylineisatricyclicantidepressantnotwidelyused intheclinicforthispurposebecauseofitsmarked sideeffectsinthedoseat whichithasto beused.However,atalowerdose,itsuseiscurrentlyinthetreatmentofpain,suchasprophylacticmigraineand cancerpain.However,studieshavedemonstrated theiractivityagainstvarioustypesofbacteriaand fungi.Duetoitsdiversified biologicalactivitycoupled withtherelativelylow priceand theisolation ofmultiresistantbacteria(MDR)mainlyatthehospitallevel,wewereencouraged to conductthisresearch.Oftendrugsthat arecapableofcleaving DNAdouble-stranded haveboth antibacterialand anti-cancereffects.Thisknowledgeand theexistenceofonlyhypothesesin theclarificationofthemechanismofactionofthisdrugistheobjectiveofthisstudyinwhich weintend to evaluatetheinvitrobiologicalactivityofthedrugamitriptylineagainstAmerican Standard CultureCollection (ATCC)referencestandard strainsand clinicalisolatesMDR,bydetermining theminimuminhibitoryconcentration(MIC),minimumbacterialconcentration (MBC),tolerancelevel,fractionalinhibitoryconcentrationindex(FICI),associated ornot with antibioticsused inclinicalpracticeand cleavageabilityofthepUC18 doublestranded plasmidDNA.
publishDate 2019
dc.date.issued.fl_str_mv 2019-08-29
dc.date.accessioned.fl_str_mv 2021-03-04T11:08:13Z
dc.date.available.fl_str_mv 2021-03-04T11:08:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.uri.fl_str_mv http://repositorio.ufsm.br/handle/1/20377
url http://repositorio.ufsm.br/handle/1/20377
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language por
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dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
Centro de Ciências da Saúde
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciências Farmacêuticas
dc.publisher.initials.fl_str_mv UFSM
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Farmacologia
publisher.none.fl_str_mv Universidade Federal de Santa Maria
Centro de Ciências da Saúde
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