Aspectos anatomopatológicos da medula óssea na peritonite infecciosa felina
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/11756 |
Resumo: | Feline infectious peritonitis (FIP) is a highly contagious, progressive and invariably fatal viral disease of cats, and occasionally of wild felids, which results from antibody-mediated hypersensitivity reactions (types III and IV) in individuals incapable to produce a cell-mediated immune response. Although the prevalence of FIP is high worldwide, recent anatomopathological studies about this disease are scarce. Furthermore, the microscopic characteristics of the bone marrow of FIP-affected cats do not exist in the available literature. Based on this, the purpose of this dissertation is to describe possible bone marrow lesions seen in spontaneous cases of FIP. Therefore, the bone marrow collected systematically from the femoral diaphysis of 16 cats necropsied in the LPV-UFSM (Santa Maria, RS, Brazil), between January 2000 and June 2017, with a definitive diagnosis of FIP, were evaluated phenotypically (histopathology [hematoxylin and eosin] and histochemistry [Perls reaction]) and immunophenotypically (immunohistochemistry using anti-myeloid [MAC387] and anti-lymphoid [CD79αcy and CD3] markers). The results showed, regardless of the clinicopathological form of the disease (“dry” [noneffusive] or “wet” [effusive]), myeloid hyperplasia; erythroid hipoplasia; megakaryocytic dysplasia (dismegakaryocytopoiesis); and medullary plasmacytosis. In cases of “dry FIP”, but not in those of “wet PIF”, there was bone marrow and hepatic hemosiderosis. These lesions allowed establishing that cats with FIP develop myelodysplasia, a myeloproliferative lesion very similar to that reported in HIV-infected humans. It is suggested that, based on the findings described here, myelodysplasia is considered to be the main cause of hematological abnormalities observed in FIP, especially for non-regenerative anemia and thrombocytopenia, frequently developed by patients. |
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2017-09-26T13:36:16Z2017-09-26T13:36:16Z2017-07-14http://repositorio.ufsm.br/handle/1/11756Feline infectious peritonitis (FIP) is a highly contagious, progressive and invariably fatal viral disease of cats, and occasionally of wild felids, which results from antibody-mediated hypersensitivity reactions (types III and IV) in individuals incapable to produce a cell-mediated immune response. Although the prevalence of FIP is high worldwide, recent anatomopathological studies about this disease are scarce. Furthermore, the microscopic characteristics of the bone marrow of FIP-affected cats do not exist in the available literature. Based on this, the purpose of this dissertation is to describe possible bone marrow lesions seen in spontaneous cases of FIP. Therefore, the bone marrow collected systematically from the femoral diaphysis of 16 cats necropsied in the LPV-UFSM (Santa Maria, RS, Brazil), between January 2000 and June 2017, with a definitive diagnosis of FIP, were evaluated phenotypically (histopathology [hematoxylin and eosin] and histochemistry [Perls reaction]) and immunophenotypically (immunohistochemistry using anti-myeloid [MAC387] and anti-lymphoid [CD79αcy and CD3] markers). The results showed, regardless of the clinicopathological form of the disease (“dry” [noneffusive] or “wet” [effusive]), myeloid hyperplasia; erythroid hipoplasia; megakaryocytic dysplasia (dismegakaryocytopoiesis); and medullary plasmacytosis. In cases of “dry FIP”, but not in those of “wet PIF”, there was bone marrow and hepatic hemosiderosis. These lesions allowed establishing that cats with FIP develop myelodysplasia, a myeloproliferative lesion very similar to that reported in HIV-infected humans. It is suggested that, based on the findings described here, myelodysplasia is considered to be the main cause of hematological abnormalities observed in FIP, especially for non-regenerative anemia and thrombocytopenia, frequently developed by patients.Peritonite infecciosa felina (PIF) é uma doença marcadamente contagiosa, progressiva e invariavelmente fatal de gatos, ocasionalmente de felideos selvagens, que decorre de uma reação de hipersensibilidade (tipos III e IV) em um indivíduo incapaz de montar uma resposta imune celular adequada. Apesar da prevalência da peritonite infecciosa felina (PIF) ser alta em praticamente o mundo todo, estudos anatomopatológicos recentes acerca dessa doença são escassos. Não obstante, as características microscópicas da medula óssea de gatos com PIF inexistem na literatura consultada. Com base nisso, o objetivo deste estudo é descrever possíveis alterações medulares ósseas vistas em casos espontâneos de PIF. Para isso, as medulas ósseas colhidas sistematicamente da região diafisária dos fêmures de 16 gatos necropsiados no LPV-UFSM (Santa Maria, RS, Brasil), entre janeiro de 2000 e junho de 2017, e que tiveram diagnóstico definitivo de PIF, foram avaliadas fenotípica (histopatologia [hematoxilina e eosina] e histoquímica [reação de Perls]) e imunofenotipicamente (imuno-histoquímica utilizando marcadores anti-mieloide (MAC387) e anti-linfoide (CD79 αcy e CD3). Os resultados permitem afirmar que, independentemente da apresentação clinicopatológica da doença (seca ou úmida), ocorre: 1) hiperplasia mieloide; 2) hipoplasia eritroide, 3) displasia megacariocítica (dismegacariocitopoiese) e 4) plasmocitose medular. Nos casos de PIF seca, mas não naqueles de PIF úmida, há hemossiderose medular óssea e hepática. Essas alterações permitem estabelecer que gatos com PIF desenvolvem mielodisplasia, uma lesão mieloproliferativa muito semelhante àquela relatada em humanos infectados pelo HIV. Sugere-se que a partir dos achados aqui descritos, mielodisplasia seja considerada a principal responsável pelas alterações hematológicas observadas na PIF, especialmente pela anemia e trombocitopenia arregenerativas tão frequentemente desenvolvidas pelos pacientes com essa doença.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências RuraisPrograma de Pós-Graduação em Medicina VeterináriaUFSMBrasilMedicina VeterináriaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessCoronavírus felinoMedula ósseaPeritonite infecciosa felinaDoenças de gatosPatologiaFeline coronavirusBone marrowFeline infectious peritonitisDiseases of catsPathologyCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAAspectos anatomopatológicos da medula óssea na peritonite infecciosa felinaPathological aspects of the bone marrow in feline infectious peritonitisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisFighera, Rafael Almeidahttp://lattes.cnpq.br/6223365736139655Krause, Alexandrehttp://lattes.cnpq.br/7760558908777387Souza, Tatiana Mello dehttp://lattes.cnpq.br/2611358012347328http://lattes.cnpq.br/8955014668583611Luz, Flávia Serena da500500000007600e1b2f8ee-da7a-403f-acb4-f6cc848ea946d6246d31-cfde-4410-90da-86ebc202329a48b1731f-9fa9-4553-ab4a-18302b9fc514f134a7aa-0e9c-442d-ad65-3910e7a444e8reponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Aspectos anatomopatológicos da medula óssea na peritonite infecciosa felina |
dc.title.alternative.eng.fl_str_mv |
Pathological aspects of the bone marrow in feline infectious peritonitis |
title |
Aspectos anatomopatológicos da medula óssea na peritonite infecciosa felina |
spellingShingle |
Aspectos anatomopatológicos da medula óssea na peritonite infecciosa felina Luz, Flávia Serena da Coronavírus felino Medula óssea Peritonite infecciosa felina Doenças de gatos Patologia Feline coronavirus Bone marrow Feline infectious peritonitis Diseases of cats Pathology CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Aspectos anatomopatológicos da medula óssea na peritonite infecciosa felina |
title_full |
Aspectos anatomopatológicos da medula óssea na peritonite infecciosa felina |
title_fullStr |
Aspectos anatomopatológicos da medula óssea na peritonite infecciosa felina |
title_full_unstemmed |
Aspectos anatomopatológicos da medula óssea na peritonite infecciosa felina |
title_sort |
Aspectos anatomopatológicos da medula óssea na peritonite infecciosa felina |
author |
Luz, Flávia Serena da |
author_facet |
Luz, Flávia Serena da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Fighera, Rafael Almeida |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6223365736139655 |
dc.contributor.referee1.fl_str_mv |
Krause, Alexandre |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/7760558908777387 |
dc.contributor.referee2.fl_str_mv |
Souza, Tatiana Mello de |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/2611358012347328 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8955014668583611 |
dc.contributor.author.fl_str_mv |
Luz, Flávia Serena da |
contributor_str_mv |
Fighera, Rafael Almeida Krause, Alexandre Souza, Tatiana Mello de |
dc.subject.por.fl_str_mv |
Coronavírus felino Medula óssea Peritonite infecciosa felina Doenças de gatos Patologia |
topic |
Coronavírus felino Medula óssea Peritonite infecciosa felina Doenças de gatos Patologia Feline coronavirus Bone marrow Feline infectious peritonitis Diseases of cats Pathology CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Feline coronavirus Bone marrow Feline infectious peritonitis Diseases of cats Pathology |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Feline infectious peritonitis (FIP) is a highly contagious, progressive and invariably fatal viral disease of cats, and occasionally of wild felids, which results from antibody-mediated hypersensitivity reactions (types III and IV) in individuals incapable to produce a cell-mediated immune response. Although the prevalence of FIP is high worldwide, recent anatomopathological studies about this disease are scarce. Furthermore, the microscopic characteristics of the bone marrow of FIP-affected cats do not exist in the available literature. Based on this, the purpose of this dissertation is to describe possible bone marrow lesions seen in spontaneous cases of FIP. Therefore, the bone marrow collected systematically from the femoral diaphysis of 16 cats necropsied in the LPV-UFSM (Santa Maria, RS, Brazil), between January 2000 and June 2017, with a definitive diagnosis of FIP, were evaluated phenotypically (histopathology [hematoxylin and eosin] and histochemistry [Perls reaction]) and immunophenotypically (immunohistochemistry using anti-myeloid [MAC387] and anti-lymphoid [CD79αcy and CD3] markers). The results showed, regardless of the clinicopathological form of the disease (“dry” [noneffusive] or “wet” [effusive]), myeloid hyperplasia; erythroid hipoplasia; megakaryocytic dysplasia (dismegakaryocytopoiesis); and medullary plasmacytosis. In cases of “dry FIP”, but not in those of “wet PIF”, there was bone marrow and hepatic hemosiderosis. These lesions allowed establishing that cats with FIP develop myelodysplasia, a myeloproliferative lesion very similar to that reported in HIV-infected humans. It is suggested that, based on the findings described here, myelodysplasia is considered to be the main cause of hematological abnormalities observed in FIP, especially for non-regenerative anemia and thrombocytopenia, frequently developed by patients. |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-09-26T13:36:16Z |
dc.date.available.fl_str_mv |
2017-09-26T13:36:16Z |
dc.date.issued.fl_str_mv |
2017-07-14 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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http://repositorio.ufsm.br/handle/1/11756 |
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http://repositorio.ufsm.br/handle/1/11756 |
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por |
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por |
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500500000007 |
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600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Medicina Veterinária |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Medicina Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
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