Caracterização dos genes que codificam selenoproteínas em peixe-zebra (Danio rerio)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/22093 |
Resumo: | Selenoproteins are selenocysteine (Sec)-containing proteins that exhibit numerous physiological functions, including redox homeostasis maintenance, thyroid hormones metabolism, selenium transport and chaperone-like activities. Compared to other organisms, zebrafish presents the highest number of selenoprotein-coding genes identified to date, and almost all selenoproteins found in human are present in the fish. Despite this, the variety of selenoproteins in zebrafish is little explored. In order to identify potential targets for translational studies, herein we analyzed the transcriptional status of selenoprotein genes in different tissues and life stages of zebrafish. Using RNA-seq data set from Sequence Reads Archive (SRA) experiments published at the DNA Data Bank of Japan (DDBJ), we determined the transcriptional levels of the 37 selenoproteins-coding sequences in brain, heart, gills, liver and skeletal muscle, comparing them throughout aging. Zebrafish selenoprotein-coding genes were confirmed by the presence of 3'-UTR SECIS structure in selenoprotein-coding mRNAs. Out of 37 selenoprotein genes analyzed here, we were unable to found a proper SECIS sequence for Selenoprotein J gene (selenoj). Overall, our transcriptome queries suggest all selenoprotein genes are actively transcribed in the tissues, with the exception of Selenoprotein E gene (selenoe). Finally, RNA-seq search revealed brain has the highest selenoprotein diversity and the transcription of 20 genes varied accordingly to age, with a peak at 16-month-old zebrafish brain. Collectively, the data from the present study support zebrafish and specially zebrafish adult brain as potent model organism for studies towards selenium and selenoproteins, focusing particularly on dio2, gpx1a, gpx4a, gpx4b, msrb1b, selenof, selenop, selenot1a, selenot2 selenow1, and selenow2a genes as targets for future translational studies. |
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2021-08-27T21:27:00Z2021-08-27T21:27:00Z2020-02-21http://repositorio.ufsm.br/handle/1/22093Selenoproteins are selenocysteine (Sec)-containing proteins that exhibit numerous physiological functions, including redox homeostasis maintenance, thyroid hormones metabolism, selenium transport and chaperone-like activities. Compared to other organisms, zebrafish presents the highest number of selenoprotein-coding genes identified to date, and almost all selenoproteins found in human are present in the fish. Despite this, the variety of selenoproteins in zebrafish is little explored. In order to identify potential targets for translational studies, herein we analyzed the transcriptional status of selenoprotein genes in different tissues and life stages of zebrafish. Using RNA-seq data set from Sequence Reads Archive (SRA) experiments published at the DNA Data Bank of Japan (DDBJ), we determined the transcriptional levels of the 37 selenoproteins-coding sequences in brain, heart, gills, liver and skeletal muscle, comparing them throughout aging. Zebrafish selenoprotein-coding genes were confirmed by the presence of 3'-UTR SECIS structure in selenoprotein-coding mRNAs. Out of 37 selenoprotein genes analyzed here, we were unable to found a proper SECIS sequence for Selenoprotein J gene (selenoj). Overall, our transcriptome queries suggest all selenoprotein genes are actively transcribed in the tissues, with the exception of Selenoprotein E gene (selenoe). Finally, RNA-seq search revealed brain has the highest selenoprotein diversity and the transcription of 20 genes varied accordingly to age, with a peak at 16-month-old zebrafish brain. Collectively, the data from the present study support zebrafish and specially zebrafish adult brain as potent model organism for studies towards selenium and selenoproteins, focusing particularly on dio2, gpx1a, gpx4a, gpx4b, msrb1b, selenof, selenop, selenot1a, selenot2 selenow1, and selenow2a genes as targets for future translational studies.As selenoproteínas são proteínas contendo selenocisteína (Sec) que exibem inúmeras funções fisiológicas, incluindo manutenção da homeostase redox, metabolismo dos hormônios da tireóide, transporte de selênio e atividades do tipo chaperona. Comparado a outros vertebrados, o peixe-zebra apresenta o maior número de genes codificadores de selenoproteínas identificados até o momento, e quase todas as selenoproteínas encontradas em humanos estão presentes nos peixes. Apesar disso, a variedade de selenoproteínas no peixe-zebra é pouco explorada. Para identificar alvos em potencial para estudos translacionais, foi analizado o status transcricional dos genes de selenoproteínas em diferentes tecidos e estágios da vida do peixe-zebra. Usando o conjunto de dados RNA-seq dos experimentos em Sequence Reads Archive (SRA) publicados no DNA Data Bank of Japan (DDBJ), foram determinados os níveis transcricionais das 37 sequências codificadoras de selenoproteínas em cérebro, coração, brânquias, fígado e músculo esquelético, comparando-os ao longo de diferentes idades. Os genes que codificam as selenoproteínas de peixe-zebra foram confirmados pela presença da estrutura SECIS na região não traduzida 3' dos mRNAs que codificam a selenoproteínas. Dos 37 genes das selenoproteínas analisados aqui, não foi encontrada uma sequência SECIS para o gene da selenoproteína J (selenoj). No geral, as consultas ao transcriptoma mostraram que todos os genes de selenoproteínas são ativamente transcritos nos tecidos analisados, com exceção do gene para selenoproteína E (selenoe). A pesquisa com RNA-seq revelou também que o cérebro é o tecido com maior diversidade de selenoproteínas, e que a transcrição de 20 genes varia de acordo com a idade, atingindo um pico no nível de transcrição no cérebro de peixe-zebra aos 16 meses de vida. Coletivamente, os dados do presente estudo identificam o peixe-zebra e, especialmente, o cérebro de peixes-zebra adultos como potencial tecido modelo para estudos sobre selênio e selenoproteínas, focando particularmente nos genes dio2, gpx1a, gpx4a, gpx4b, msrb1b, selenof, selenop, selenot1a, selenot2 selenow1 e selenow2a como alvos em futuros estudos translacionais.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBrasilBioquímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessTranscriptomaSECISSelenocisteínaSelênioSeleniumTranscriptomeSelenocysteinZebrafishCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICACaracterização dos genes que codificam selenoproteínas em peixe-zebra (Danio rerio)Characterization of selenoprotein coding genes in zebrafish (Danio rerio)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisBarbosa, Nilda Berenice de Vargashttp://lattes.cnpq.br/5901511067144019Araújo, Daniel Mendes Pereira Ardisson deSchuch, André PassagliaFolmer, Vanderleihttp://lattes.cnpq.br/4363474436933678Macedo, Gabriel Teixeira de20080000000260060060060060060087b11d6d-46b4-49bc-b979-5342a4cd48ffe87dcf0f-8c9c-4446-8b91-e30f1322777a9aaaf111-4d9d-4af1-bd3a-33bee7d19ad86ee75042-a06b-48e6-a37c-ef9600da01f885335f1d-646b-4ef6-b360-9b60112e283freponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGCBBT_2020_MACEDO_GABRIEL.pdfDIS_PPGCBBT_2020_MACEDO_GABRIEL.pdfDissertaçãoapplication/pdf3251793http://repositorio.ufsm.br/bitstream/1/22093/1/DIS_PPGCBBT_2020_MACEDO_GABRIEL.pdf475cabe2390d8442834a7ef3a58c71faMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Caracterização dos genes que codificam selenoproteínas em peixe-zebra (Danio rerio) |
dc.title.alternative.eng.fl_str_mv |
Characterization of selenoprotein coding genes in zebrafish (Danio rerio) |
title |
Caracterização dos genes que codificam selenoproteínas em peixe-zebra (Danio rerio) |
spellingShingle |
Caracterização dos genes que codificam selenoproteínas em peixe-zebra (Danio rerio) Macedo, Gabriel Teixeira de Transcriptoma SECIS Selenocisteína Selênio Selenium Transcriptome Selenocystein Zebrafish CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Caracterização dos genes que codificam selenoproteínas em peixe-zebra (Danio rerio) |
title_full |
Caracterização dos genes que codificam selenoproteínas em peixe-zebra (Danio rerio) |
title_fullStr |
Caracterização dos genes que codificam selenoproteínas em peixe-zebra (Danio rerio) |
title_full_unstemmed |
Caracterização dos genes que codificam selenoproteínas em peixe-zebra (Danio rerio) |
title_sort |
Caracterização dos genes que codificam selenoproteínas em peixe-zebra (Danio rerio) |
author |
Macedo, Gabriel Teixeira de |
author_facet |
Macedo, Gabriel Teixeira de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Barbosa, Nilda Berenice de Vargas |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5901511067144019 |
dc.contributor.advisor-co1.fl_str_mv |
Araújo, Daniel Mendes Pereira Ardisson de |
dc.contributor.referee1.fl_str_mv |
Schuch, André Passaglia |
dc.contributor.referee2.fl_str_mv |
Folmer, Vanderlei |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4363474436933678 |
dc.contributor.author.fl_str_mv |
Macedo, Gabriel Teixeira de |
contributor_str_mv |
Barbosa, Nilda Berenice de Vargas Araújo, Daniel Mendes Pereira Ardisson de Schuch, André Passaglia Folmer, Vanderlei |
dc.subject.por.fl_str_mv |
Transcriptoma SECIS Selenocisteína Selênio Selenium |
topic |
Transcriptoma SECIS Selenocisteína Selênio Selenium Transcriptome Selenocystein Zebrafish CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Transcriptome Selenocystein Zebrafish |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Selenoproteins are selenocysteine (Sec)-containing proteins that exhibit numerous physiological functions, including redox homeostasis maintenance, thyroid hormones metabolism, selenium transport and chaperone-like activities. Compared to other organisms, zebrafish presents the highest number of selenoprotein-coding genes identified to date, and almost all selenoproteins found in human are present in the fish. Despite this, the variety of selenoproteins in zebrafish is little explored. In order to identify potential targets for translational studies, herein we analyzed the transcriptional status of selenoprotein genes in different tissues and life stages of zebrafish. Using RNA-seq data set from Sequence Reads Archive (SRA) experiments published at the DNA Data Bank of Japan (DDBJ), we determined the transcriptional levels of the 37 selenoproteins-coding sequences in brain, heart, gills, liver and skeletal muscle, comparing them throughout aging. Zebrafish selenoprotein-coding genes were confirmed by the presence of 3'-UTR SECIS structure in selenoprotein-coding mRNAs. Out of 37 selenoprotein genes analyzed here, we were unable to found a proper SECIS sequence for Selenoprotein J gene (selenoj). Overall, our transcriptome queries suggest all selenoprotein genes are actively transcribed in the tissues, with the exception of Selenoprotein E gene (selenoe). Finally, RNA-seq search revealed brain has the highest selenoprotein diversity and the transcription of 20 genes varied accordingly to age, with a peak at 16-month-old zebrafish brain. Collectively, the data from the present study support zebrafish and specially zebrafish adult brain as potent model organism for studies towards selenium and selenoproteins, focusing particularly on dio2, gpx1a, gpx4a, gpx4b, msrb1b, selenof, selenop, selenot1a, selenot2 selenow1, and selenow2a genes as targets for future translational studies. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020-02-21 |
dc.date.accessioned.fl_str_mv |
2021-08-27T21:27:00Z |
dc.date.available.fl_str_mv |
2021-08-27T21:27:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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http://repositorio.ufsm.br/handle/1/22093 |
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http://repositorio.ufsm.br/handle/1/22093 |
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por |
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200800000002 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
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Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
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UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Bioquímica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
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