Performance da soja que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac para controle de lepidópteros-praga e manejo da resistência de insetos
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/23558 |
Resumo: | Genetically modified soybeans expressing Bacillus thuringiensis Berliner (Bt) proteins have revolutionized pest management throughout South America due to efficacy against major soybean pests. The pyramided genetically modified soybean [Glycine max L. (Merr.)] MON87751 × MON87708 × MON87701 × MON89788, expressing Cry1Ac, Cry1A.105 and Cry2Ab2 from Bacillus thuringiensis Berliner was approved for commercial use in Brazil. In this study were conducted laboratory, greenhouse and field studies to assess the performance of this Bt soybean technology against key soybean lepidopteran pests and support insect resistance management plans. Laboratory studies were carried out with purified proteins Cry1Ac, Cry1A.105 and Cry2Ab2 in artificial diet and soybean leaf discs to assess the susceptibility of the species, and greenhouse and field to assess the efficacy of the technology in high infestation and unde natural infestations in field conditions. Neonates of Anticarsia gemmatalis (Hübner), Chrysodeixis includens (Walker) and Helicoverpa armigera (Hübner) were susceptible to Cry1Ac (LC50 from 0.15 to 5.07), Cry1A.105 (LC50 from 0.79 to 48.22) and Cry2Ab2 (LC50 from 1.24 to 8.36) Bt proteins in diet-overlay bioassays. In laboratory leaf disc bioassays and greenhouse trials, MON87751 × MON87708 × MON87701 × MON89788 soybean as well as the individual components were effective in controlling A. gemmatalis, C. includens and H. armigera. Similarly, under field conditions, the pyramided event expressing Cry1A.105, Cry2Ab2 and Cry1Ac was highly effective at protecting soybean against C. includens. The individual proteins expressed by genetically modified soybean MON87751 × MON87708 × MON87701 × MON89788 killed all or nearly all the susceptible A. gemmatalis, C. includens and H. armigera, fulfilling an important pyramided Bt criterion which is the concept of redundant mortality, as a strategy for delaying resistance and sustaining the benefits of Bt soybean in Brazil. |
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2022-01-17T19:08:53Z2022-01-17T19:08:53Z2020-03-05http://repositorio.ufsm.br/handle/1/23558Genetically modified soybeans expressing Bacillus thuringiensis Berliner (Bt) proteins have revolutionized pest management throughout South America due to efficacy against major soybean pests. The pyramided genetically modified soybean [Glycine max L. (Merr.)] MON87751 × MON87708 × MON87701 × MON89788, expressing Cry1Ac, Cry1A.105 and Cry2Ab2 from Bacillus thuringiensis Berliner was approved for commercial use in Brazil. In this study were conducted laboratory, greenhouse and field studies to assess the performance of this Bt soybean technology against key soybean lepidopteran pests and support insect resistance management plans. Laboratory studies were carried out with purified proteins Cry1Ac, Cry1A.105 and Cry2Ab2 in artificial diet and soybean leaf discs to assess the susceptibility of the species, and greenhouse and field to assess the efficacy of the technology in high infestation and unde natural infestations in field conditions. Neonates of Anticarsia gemmatalis (Hübner), Chrysodeixis includens (Walker) and Helicoverpa armigera (Hübner) were susceptible to Cry1Ac (LC50 from 0.15 to 5.07), Cry1A.105 (LC50 from 0.79 to 48.22) and Cry2Ab2 (LC50 from 1.24 to 8.36) Bt proteins in diet-overlay bioassays. In laboratory leaf disc bioassays and greenhouse trials, MON87751 × MON87708 × MON87701 × MON89788 soybean as well as the individual components were effective in controlling A. gemmatalis, C. includens and H. armigera. Similarly, under field conditions, the pyramided event expressing Cry1A.105, Cry2Ab2 and Cry1Ac was highly effective at protecting soybean against C. includens. The individual proteins expressed by genetically modified soybean MON87751 × MON87708 × MON87701 × MON89788 killed all or nearly all the susceptible A. gemmatalis, C. includens and H. armigera, fulfilling an important pyramided Bt criterion which is the concept of redundant mortality, as a strategy for delaying resistance and sustaining the benefits of Bt soybean in Brazil.A soja geneticamente modificada que expressa genes da bactéria Bacillus thuringiensis Berliner (Bt) tem revolucionado o manejo de pragas na América do Sul, devido sua eficácia contra as principais lagartas desfolhadoras da soja. O evento de soja expressando Cry1A.105, Cry1Ac e Cry2Ab2 (evento MON87751 × MON87708 × MON87701 × MON89788) foi aprovado pela CTNBio para uso comercial, sendo essa uma nova opção para o Manejo Integrado de Pragas e Manejo de Resistência de Insetos. Para entender o valor dessa nova tecnologia no manejo de lagartas defolhadoras da soja, foram realizados estudos em laboratório, casa de vegetação e campo para avaliar a performance da soja MON87751 × MON87708 × MON87701 × MON89788 no controle de Anticarsia gemmatalis (Hübner), Chrysodeixis includens (Walker) e Helicoverpa armigera (Hübner). Em laboratório, estudos com as proteínas purificadas Cry1A.105, Cry1Ac e Cry2Ab2 (em dieta artificial) e disco de folha dos eventos (MON87751 × MON87701, MON87751, MON87701, A845232 e A844620) foram realizados para avaliar a suscetibilidade das espécies. Em casa de vegetação os estudos foram realizados sobre condições de alta infestação e a campo sobre infestação natural. Neonatas de A. gemmatalis, C. includens e H. armigera foram suscetíveis as proteínas Cry1Ac: CL50 (0,15 – 5,07), Cry1A.105: CL50 (0,79 – 48,22) e Cry2Ab2: CL50 (1,24 – 8,36). Nos bioensaios com discos de folha e em casa de vegetação a soja MON87751 × MON87708 × MON87701 × MON89788 e seus eventos individuais foram eficazes no controle A. gemmatalis, C. includens e H. armigera. De modo similar, em condições de campo, a soja MON87751 × MON87708 × MON87701 × MON89788 foi eficaz contra C. includens. As proteínas Cry1A.105, Cry1Ac, Cry2Ab2 expressas isoladamente e no evento de soja MON87751 × MON87708 × MON87701 × MON89788 foram eficazes para controle de A. gemmatalis, C. includens e H. armigera, atendendo um importante critério, o de pirâmide de genes, que é o conceito de mortalidade redundante, para o manejo da resistência de insetos.porUniversidade Federal de Santa MariaCentro de Ciências RuraisPrograma de Pós-Graduação em AgronomiaUFSMBrasilAgronomiaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessSoja transgênicaBacillus thuringiensis (Bt)EficáciaManejo da resistência de insetosTransgenic soybeanEfficacyInsect resistance managementCNPQ::CIENCIAS AGRARIAS::AGRONOMIAPerformance da soja que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac para controle de lepidópteros-praga e manejo da resistência de insetosPerformance of soybean expressing insecticidal proteins Cry1A.105, Cry2Ab2 and Cry1Ac for control of lepidopteran pests and insect resistance managementinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisBernardi, Oderleihttp://lattes.cnpq.br/3085175517473196Salvadori, José RobertoDourado, Patrick Marqueshttp://lattes.cnpq.br/4803859362490120Bacalhau, Fabiana Britti50010000000960060060050431674-7373-4bd1-a195-531da03e42033a83bf43-6dd9-428f-8411-6a9b248353958f86fb86-25e7-4184-a1e5-bcb1daa7369dbbb4e67e-958d-46d6-8ab5-888a0cbdc716reponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Performance da soja que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac para controle de lepidópteros-praga e manejo da resistência de insetos |
dc.title.alternative.eng.fl_str_mv |
Performance of soybean expressing insecticidal proteins Cry1A.105, Cry2Ab2 and Cry1Ac for control of lepidopteran pests and insect resistance management |
title |
Performance da soja que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac para controle de lepidópteros-praga e manejo da resistência de insetos |
spellingShingle |
Performance da soja que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac para controle de lepidópteros-praga e manejo da resistência de insetos Bacalhau, Fabiana Britti Soja transgênica Bacillus thuringiensis (Bt) Eficácia Manejo da resistência de insetos Transgenic soybean Efficacy Insect resistance management CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Performance da soja que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac para controle de lepidópteros-praga e manejo da resistência de insetos |
title_full |
Performance da soja que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac para controle de lepidópteros-praga e manejo da resistência de insetos |
title_fullStr |
Performance da soja que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac para controle de lepidópteros-praga e manejo da resistência de insetos |
title_full_unstemmed |
Performance da soja que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac para controle de lepidópteros-praga e manejo da resistência de insetos |
title_sort |
Performance da soja que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac para controle de lepidópteros-praga e manejo da resistência de insetos |
author |
Bacalhau, Fabiana Britti |
author_facet |
Bacalhau, Fabiana Britti |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Bernardi, Oderlei |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3085175517473196 |
dc.contributor.referee1.fl_str_mv |
Salvadori, José Roberto |
dc.contributor.referee2.fl_str_mv |
Dourado, Patrick Marques |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4803859362490120 |
dc.contributor.author.fl_str_mv |
Bacalhau, Fabiana Britti |
contributor_str_mv |
Bernardi, Oderlei Salvadori, José Roberto Dourado, Patrick Marques |
dc.subject.por.fl_str_mv |
Soja transgênica Bacillus thuringiensis (Bt) Eficácia Manejo da resistência de insetos |
topic |
Soja transgênica Bacillus thuringiensis (Bt) Eficácia Manejo da resistência de insetos Transgenic soybean Efficacy Insect resistance management CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
dc.subject.eng.fl_str_mv |
Transgenic soybean Efficacy Insect resistance management |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
Genetically modified soybeans expressing Bacillus thuringiensis Berliner (Bt) proteins have revolutionized pest management throughout South America due to efficacy against major soybean pests. The pyramided genetically modified soybean [Glycine max L. (Merr.)] MON87751 × MON87708 × MON87701 × MON89788, expressing Cry1Ac, Cry1A.105 and Cry2Ab2 from Bacillus thuringiensis Berliner was approved for commercial use in Brazil. In this study were conducted laboratory, greenhouse and field studies to assess the performance of this Bt soybean technology against key soybean lepidopteran pests and support insect resistance management plans. Laboratory studies were carried out with purified proteins Cry1Ac, Cry1A.105 and Cry2Ab2 in artificial diet and soybean leaf discs to assess the susceptibility of the species, and greenhouse and field to assess the efficacy of the technology in high infestation and unde natural infestations in field conditions. Neonates of Anticarsia gemmatalis (Hübner), Chrysodeixis includens (Walker) and Helicoverpa armigera (Hübner) were susceptible to Cry1Ac (LC50 from 0.15 to 5.07), Cry1A.105 (LC50 from 0.79 to 48.22) and Cry2Ab2 (LC50 from 1.24 to 8.36) Bt proteins in diet-overlay bioassays. In laboratory leaf disc bioassays and greenhouse trials, MON87751 × MON87708 × MON87701 × MON89788 soybean as well as the individual components were effective in controlling A. gemmatalis, C. includens and H. armigera. Similarly, under field conditions, the pyramided event expressing Cry1A.105, Cry2Ab2 and Cry1Ac was highly effective at protecting soybean against C. includens. The individual proteins expressed by genetically modified soybean MON87751 × MON87708 × MON87701 × MON89788 killed all or nearly all the susceptible A. gemmatalis, C. includens and H. armigera, fulfilling an important pyramided Bt criterion which is the concept of redundant mortality, as a strategy for delaying resistance and sustaining the benefits of Bt soybean in Brazil. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020-03-05 |
dc.date.accessioned.fl_str_mv |
2022-01-17T19:08:53Z |
dc.date.available.fl_str_mv |
2022-01-17T19:08:53Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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http://repositorio.ufsm.br/handle/1/23558 |
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http://repositorio.ufsm.br/handle/1/23558 |
dc.language.iso.fl_str_mv |
por |
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500100000009 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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Universidade Federal de Santa Maria Centro de Ciências Rurais |
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Programa de Pós-Graduação em Agronomia |
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UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Agronomia |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
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