Produção de metabólitos antifúngicos e antibacterianos a partir do fungo Diaporthe schini
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/18735 |
Resumo: | Due to the emergence of resistance to the drugs used to treat diseases caused by fungi and bacteria, there is a growing need to identify new compounds that can be used in medicines. The compounds produced by endophytic fungi are a promising source in the production of metabolites with antifungal and antibacterial action. Thus, the objective of this work was the production of metabolites from the submerged fermentation of the fungus Diaporthe schini, evaluation of the in vitro antibacterial and antifungal activity of the extract from the fermentation and separation and characterization of these metabolites. Among the microorganisms tested, the extracts had a greater inhibitory action on the gram-positive bacteria Staphylococcus epidermidis and the gram-negative Enterobacter aerogenes and Klebsiella pneumoniae and on the fungus Candida krusei. The extract from the hexane extraction was separated into fractions by column chromatography and the main biologically active substances characterized by GC-MS were: 13-Docosenamide, (Z); 2-Hexadecene, 3,7,11,15-tetramethyl; 9-Octadecenamide and 11-Octadecenoic acid, methyl ester. Until now, there is no reports on literature related to the identification of antibacterial and antifungal metabolites produced by submerged fermentation of Diaporthe schini. This work is an initial study where the conditions of the process can be optimized through of the production of a specific compound and can be a promising source for new drugs. |
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2019-10-31T20:46:10Z2019-10-31T20:46:10Z2018-08-23http://repositorio.ufsm.br/handle/1/18735Due to the emergence of resistance to the drugs used to treat diseases caused by fungi and bacteria, there is a growing need to identify new compounds that can be used in medicines. The compounds produced by endophytic fungi are a promising source in the production of metabolites with antifungal and antibacterial action. Thus, the objective of this work was the production of metabolites from the submerged fermentation of the fungus Diaporthe schini, evaluation of the in vitro antibacterial and antifungal activity of the extract from the fermentation and separation and characterization of these metabolites. Among the microorganisms tested, the extracts had a greater inhibitory action on the gram-positive bacteria Staphylococcus epidermidis and the gram-negative Enterobacter aerogenes and Klebsiella pneumoniae and on the fungus Candida krusei. The extract from the hexane extraction was separated into fractions by column chromatography and the main biologically active substances characterized by GC-MS were: 13-Docosenamide, (Z); 2-Hexadecene, 3,7,11,15-tetramethyl; 9-Octadecenamide and 11-Octadecenoic acid, methyl ester. Until now, there is no reports on literature related to the identification of antibacterial and antifungal metabolites produced by submerged fermentation of Diaporthe schini. This work is an initial study where the conditions of the process can be optimized through of the production of a specific compound and can be a promising source for new drugs.Devido ao surgimento de resistência aos medicamentos usados no tratamento de doenças causadas por fungos e por bactérias, há uma necessidade crescente da identificação de novos compostos que possam ser utilizados em medicamentos. Os compostos produzidos por fungos endófitos são uma fonte promissora na produção de metabólitos com ação antifúngica e antibacteriana. Dessa forma, o objetivo desse trabalho foi a produção de metabólitos a partir de fermentação submersa do fungo Diaporthe schini, avaliação da atividade antibacteriana e antifúngica in vitro do extrato proveniente da fermentação, separação e caracterização desses metabólitos. Dentre os microrganismos testados, os extratos apresentaram maior ação inibitória sobre a bactéria gram-positiva Staphylococcus epidermidis e as gram-negativas Enterobacter aerogenes e Klebsiella pneumoniae e sobre o fungo Candida krusei. O extrato proveniente da extração com hexano foi separado em frações por cromatografia em coluna e as principais substâncias com efeito biológico caracterizadas por GC-MS foram: 13-Docosenamide, (Z); 2-Hexadecene, 3,7,11,15-tetramethyl; 9- Octadecenamide e 11-Octadecenoic acid, methyl ester. Até o presente momento não se tem relatos da literatura relacionados a identificação de metabólitos antibacterianos e antifúngicos produzidos por fermentação submersa de Diaporthe schini. Este trabalho é um estudo inicial onde as condições do processo podem ser otimizadas buscando a produção de um composto em específico, podendo ser uma fonte promissora para a obtenção de novos medicamentos.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqporUniversidade Federal de Santa MariaCentro de TecnologiaPrograma de Pós-Graduação em Engenharia QuímicaUFSMBrasilEngenharia QuímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessFermentação submersaDiaporthe schiniAntifúngicoAntibacterianoSubmerged fermentationDiaporthe schiniAntifungalAntibacterialCNPQ::ENGENHARIAS::ENGENHARIA QUIMICAProdução de metabólitos antifúngicos e antibacterianos a partir do fungo Diaporthe schiniProduction of antifungal and antibacterial metabolites from Diaporthe Schiniinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisKuhn, Raquel Cristinehttp://lattes.cnpq.br/1479407050883691Jahn, Sérgio Luizhttp://lattes.cnpq.br/7735147410610776Terra, Lisiane de Marsillachttp://lattes.cnpq.br/3695292052760336Prá, Valéria Dalhttp://lattes.cnpq.br/1873977455804734http://lattes.cnpq.br/8915760164601145Reis, Caroline Medeiros dos3006000000066003528ba34-a916-462d-8700-48f0999fc462b5ae19af-401e-439a-8165-f48d9a24c50a7ed6234a-3492-494c-8429-54b9c9867d49c416be98-0cc2-4c4b-8c16-d5e696ffc6e3a6a53ab0-3d10-4fd0-b328-53073d7e0a86reponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGEQ_2018_REIS_CAROLINE.pdfDIS_PPGEQ_2018_REIS_CAROLINE.pdfDissertação de Mestradoapplication/pdf618739http://repositorio.ufsm.br/bitstream/1/18735/1/DIS_PPGEQ_2018_REIS_CAROLINE.pdf77c62f0faaf8da0244edbfd7986258c6MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/18735/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-816http://repositorio.ufsm.br/bitstream/1/18735/3/license.txtf8fcb28efb1c8cf0dc096bec902bf4c4MD53TEXTDIS_PPGEQ_2018_REIS_CAROLINE.pdf.txtDIS_PPGEQ_2018_REIS_CAROLINE.pdf.txtExtracted texttext/plain89636http://repositorio.ufsm.br/bitstream/1/18735/4/DIS_PPGEQ_2018_REIS_CAROLINE.pdf.txteee426442dfa8e9e22e28db382b1f49bMD54THUMBNAILDIS_PPGEQ_2018_REIS_CAROLINE.pdf.jpgDIS_PPGEQ_2018_REIS_CAROLINE.pdf.jpgIM Thumbnailimage/jpeg4395http://repositorio.ufsm.br/bitstream/1/18735/5/DIS_PPGEQ_2018_REIS_CAROLINE.pdf.jpgcdc63c7cf1176009e06cdc2633c97257MD551/187352019-11-01 03:01:35.497oai:repositorio.ufsm.br:1/18735Q3JlYXRpdmUgQ29tbW9ucw==Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestouvidoria@ufsm.bropendoar:39132019-11-01T06:01:35Repositório Institucional Manancial UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Produção de metabólitos antifúngicos e antibacterianos a partir do fungo Diaporthe schini |
dc.title.alternative.eng.fl_str_mv |
Production of antifungal and antibacterial metabolites from Diaporthe Schini |
title |
Produção de metabólitos antifúngicos e antibacterianos a partir do fungo Diaporthe schini |
spellingShingle |
Produção de metabólitos antifúngicos e antibacterianos a partir do fungo Diaporthe schini Reis, Caroline Medeiros dos Fermentação submersa Diaporthe schini Antifúngico Antibacteriano Submerged fermentation Diaporthe schini Antifungal Antibacterial CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
title_short |
Produção de metabólitos antifúngicos e antibacterianos a partir do fungo Diaporthe schini |
title_full |
Produção de metabólitos antifúngicos e antibacterianos a partir do fungo Diaporthe schini |
title_fullStr |
Produção de metabólitos antifúngicos e antibacterianos a partir do fungo Diaporthe schini |
title_full_unstemmed |
Produção de metabólitos antifúngicos e antibacterianos a partir do fungo Diaporthe schini |
title_sort |
Produção de metabólitos antifúngicos e antibacterianos a partir do fungo Diaporthe schini |
author |
Reis, Caroline Medeiros dos |
author_facet |
Reis, Caroline Medeiros dos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Kuhn, Raquel Cristine |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1479407050883691 |
dc.contributor.advisor-co1.fl_str_mv |
Jahn, Sérgio Luiz |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/7735147410610776 |
dc.contributor.referee1.fl_str_mv |
Terra, Lisiane de Marsillac |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/3695292052760336 |
dc.contributor.referee2.fl_str_mv |
Prá, Valéria Dal |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/1873977455804734 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8915760164601145 |
dc.contributor.author.fl_str_mv |
Reis, Caroline Medeiros dos |
contributor_str_mv |
Kuhn, Raquel Cristine Jahn, Sérgio Luiz Terra, Lisiane de Marsillac Prá, Valéria Dal |
dc.subject.por.fl_str_mv |
Fermentação submersa Diaporthe schini Antifúngico Antibacteriano |
topic |
Fermentação submersa Diaporthe schini Antifúngico Antibacteriano Submerged fermentation Diaporthe schini Antifungal Antibacterial CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
dc.subject.eng.fl_str_mv |
Submerged fermentation Diaporthe schini Antifungal Antibacterial |
dc.subject.cnpq.fl_str_mv |
CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
description |
Due to the emergence of resistance to the drugs used to treat diseases caused by fungi and bacteria, there is a growing need to identify new compounds that can be used in medicines. The compounds produced by endophytic fungi are a promising source in the production of metabolites with antifungal and antibacterial action. Thus, the objective of this work was the production of metabolites from the submerged fermentation of the fungus Diaporthe schini, evaluation of the in vitro antibacterial and antifungal activity of the extract from the fermentation and separation and characterization of these metabolites. Among the microorganisms tested, the extracts had a greater inhibitory action on the gram-positive bacteria Staphylococcus epidermidis and the gram-negative Enterobacter aerogenes and Klebsiella pneumoniae and on the fungus Candida krusei. The extract from the hexane extraction was separated into fractions by column chromatography and the main biologically active substances characterized by GC-MS were: 13-Docosenamide, (Z); 2-Hexadecene, 3,7,11,15-tetramethyl; 9-Octadecenamide and 11-Octadecenoic acid, methyl ester. Until now, there is no reports on literature related to the identification of antibacterial and antifungal metabolites produced by submerged fermentation of Diaporthe schini. This work is an initial study where the conditions of the process can be optimized through of the production of a specific compound and can be a promising source for new drugs. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-08-23 |
dc.date.accessioned.fl_str_mv |
2019-10-31T20:46:10Z |
dc.date.available.fl_str_mv |
2019-10-31T20:46:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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http://repositorio.ufsm.br/handle/1/18735 |
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http://repositorio.ufsm.br/handle/1/18735 |
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por |
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por |
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300600000006 |
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600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Tecnologia |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Engenharia Química |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Engenharia Química |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Tecnologia |
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