Efeito da exposição crônica do extrato aquoso de Ilex paraguariensis sobre o comportamento e o perfil lipídico em Caenorhabditis elegans
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/28350 |
Resumo: | Ilex paraguariensis, is a plant widely consumed in southern Brazil, Uruguay and Argentina, and well characterized for its antioxidant and stimulating properties, and could be used as modulator of fat storage in order to control obesity, a worldwide problem. Thus, the effect of chronic consumption of I. paraguariensis on fat metabolism was investigated using the nematode Caenorhabditis elegans. First was carried out the identification and quantification, by HPLC-DAD, of Ilex paraguariensis aqueous extract constituents, later, the nematodes were treated with 1 mg/ml of I. paraguariensis from L1-larvae-stage until adulthood. The extract concentration was chosen because exhibited antioxidant proprieties against juglone, a generator of reactive oxygen species, since these are elevated in fat tissue of obese people. Lipid accumulation, total body energy expenditure and behavioral parameters were analyzed. Ilex paraguariensis decreased BODIPY labeling by 63.36% compared to control without affecting behaviors related to energetic balance. Total body energy expenditure of N2 wild-type, nhr-49 and ador-1 knockout strains was performed through oxygen consumption, Ilex paraguariensis was able to increase oxygen consumption only in N2 worms. In conclusion, Ilex paraguariensis decreased fat deposits and increased consumption of oxygen in N2 worms dependent on NHR-49 and ADOR-1 pathways. |
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2023-03-23T15:14:09Z2023-03-23T15:14:09Z2016-02-25http://repositorio.ufsm.br/handle/1/28350Ilex paraguariensis, is a plant widely consumed in southern Brazil, Uruguay and Argentina, and well characterized for its antioxidant and stimulating properties, and could be used as modulator of fat storage in order to control obesity, a worldwide problem. Thus, the effect of chronic consumption of I. paraguariensis on fat metabolism was investigated using the nematode Caenorhabditis elegans. First was carried out the identification and quantification, by HPLC-DAD, of Ilex paraguariensis aqueous extract constituents, later, the nematodes were treated with 1 mg/ml of I. paraguariensis from L1-larvae-stage until adulthood. The extract concentration was chosen because exhibited antioxidant proprieties against juglone, a generator of reactive oxygen species, since these are elevated in fat tissue of obese people. Lipid accumulation, total body energy expenditure and behavioral parameters were analyzed. Ilex paraguariensis decreased BODIPY labeling by 63.36% compared to control without affecting behaviors related to energetic balance. Total body energy expenditure of N2 wild-type, nhr-49 and ador-1 knockout strains was performed through oxygen consumption, Ilex paraguariensis was able to increase oxygen consumption only in N2 worms. In conclusion, Ilex paraguariensis decreased fat deposits and increased consumption of oxygen in N2 worms dependent on NHR-49 and ADOR-1 pathways.Ilex paraguariensis é uma planta amplamente consumida no sul do Brasil, Uruguai e Argentina, e bem caracterizada por suas propriedades antioxidantes e estimulantes, e pode ser usada como um modulador dos depósitos de gordura para o controle da obesidade, um problema mundial. Portanto, o efeito do consumo crônico de Ilex paraguariensis no metabolismo de gordura foi investigado usando o nematódeo Caenorhabditis elegans. Primeiramente, foi realizado a identificação e quantificação por DAD-HPLC, dos constituintes do extrato aquoso de Ilex paraguariensis. Posteriormente, os nematódeos foram tratados com 1mg/mL de I. paraguariensis a partir do estágio L1 até a vida adulta. A concentração utilizada do extrato foi escolhida baseada em suas propriedades antioxidantes contra juglone, um gerador endógeno de espécies reativas de oxigênio, uma vez que estas estão aumentadas no tecido adiposo de pessoas obesas. O acumulo de lipídeos, o gasto total de energia e alguns parâmetros comportamentais foram analisados. Ilex paraguariensis diminuiu a fluorescência do corante BODIPY em 63,36% comparado ao controle sem afetar os comportamentos relacionados ao balanço energético. O gasto energético total da cepa selvagem N2, das mutantes nhr-49 e ador-1 foi quantificado através do consumo de oxigênio, Ilex paraguariensis aumentou o consumo de O2 apenas na cepa selvagem. Em conclusão, Ilex paraguariensis diminuiu os depósitos de lipídeos e aumentou o consumo de oxigênio na cepa selvagem, de uma forma dependente das vias NHR-49 e ADOR-1.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBrasilBioquímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessMetabolismo de gorduraLipídeosProdutos naturaisConsumo de oxigênioErva-mateFat metabolismLipidsNatural productsOxygen consumptionYerba mateCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAEfeito da exposição crônica do extrato aquoso de Ilex paraguariensis sobre o comportamento e o perfil lipídico em Caenorhabditis elegansEvaluation of the effect of chronic exposition to Ilex paraguariensis aqueous extract on the behavior and lipid profile in Caenorhabditis elegansinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisSoares, Félix Alexandre Antuneshttp://lattes.cnpq.br/8752453650114092Oliveira, Sara Marchesan deWagner, Carolinehttp://lattes.cnpq.br/0382013676927848Machado, Marina Lopes2008001000096006006006006003aef53b7-b173-4c56-a944-bed2de5e5dda121a3386-f146-43dc-9b04-c78a9b47d7d4ae9ccccd-6c23-46a1-abda-fa450ef31e4f75ee513b-7658-4252-ad23-0af0d13b7c8ereponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGBT_2016_ MACHADO_MARINA.pdfDIS_PPGBT_2016_ MACHADO_MARINA.pdfDissertação de mestradoapplication/pdf1153954http://repositorio.ufsm.br/bitstream/1/28350/1/DIS_PPGBT_2016_%20MACHADO_MARINA.pdfad58c9b0ef3620ee59e979b1ed96330eMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/28350/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/28350/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD531/283502023-03-23 12:14:09.269oai:repositorio.ufsm.br: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ório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestouvidoria@ufsm.bropendoar:39132023-03-23T15:14:09Repositório Institucional Manancial UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Efeito da exposição crônica do extrato aquoso de Ilex paraguariensis sobre o comportamento e o perfil lipídico em Caenorhabditis elegans |
dc.title.alternative.eng.fl_str_mv |
Evaluation of the effect of chronic exposition to Ilex paraguariensis aqueous extract on the behavior and lipid profile in Caenorhabditis elegans |
title |
Efeito da exposição crônica do extrato aquoso de Ilex paraguariensis sobre o comportamento e o perfil lipídico em Caenorhabditis elegans |
spellingShingle |
Efeito da exposição crônica do extrato aquoso de Ilex paraguariensis sobre o comportamento e o perfil lipídico em Caenorhabditis elegans Machado, Marina Lopes Metabolismo de gordura Lipídeos Produtos naturais Consumo de oxigênio Erva-mate Fat metabolism Lipids Natural products Oxygen consumption Yerba mate CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Efeito da exposição crônica do extrato aquoso de Ilex paraguariensis sobre o comportamento e o perfil lipídico em Caenorhabditis elegans |
title_full |
Efeito da exposição crônica do extrato aquoso de Ilex paraguariensis sobre o comportamento e o perfil lipídico em Caenorhabditis elegans |
title_fullStr |
Efeito da exposição crônica do extrato aquoso de Ilex paraguariensis sobre o comportamento e o perfil lipídico em Caenorhabditis elegans |
title_full_unstemmed |
Efeito da exposição crônica do extrato aquoso de Ilex paraguariensis sobre o comportamento e o perfil lipídico em Caenorhabditis elegans |
title_sort |
Efeito da exposição crônica do extrato aquoso de Ilex paraguariensis sobre o comportamento e o perfil lipídico em Caenorhabditis elegans |
author |
Machado, Marina Lopes |
author_facet |
Machado, Marina Lopes |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Soares, Félix Alexandre Antunes |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8752453650114092 |
dc.contributor.referee1.fl_str_mv |
Oliveira, Sara Marchesan de |
dc.contributor.referee2.fl_str_mv |
Wagner, Caroline |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0382013676927848 |
dc.contributor.author.fl_str_mv |
Machado, Marina Lopes |
contributor_str_mv |
Soares, Félix Alexandre Antunes Oliveira, Sara Marchesan de Wagner, Caroline |
dc.subject.por.fl_str_mv |
Metabolismo de gordura Lipídeos Produtos naturais Consumo de oxigênio Erva-mate |
topic |
Metabolismo de gordura Lipídeos Produtos naturais Consumo de oxigênio Erva-mate Fat metabolism Lipids Natural products Oxygen consumption Yerba mate CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Fat metabolism Lipids Natural products Oxygen consumption Yerba mate |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Ilex paraguariensis, is a plant widely consumed in southern Brazil, Uruguay and Argentina, and well characterized for its antioxidant and stimulating properties, and could be used as modulator of fat storage in order to control obesity, a worldwide problem. Thus, the effect of chronic consumption of I. paraguariensis on fat metabolism was investigated using the nematode Caenorhabditis elegans. First was carried out the identification and quantification, by HPLC-DAD, of Ilex paraguariensis aqueous extract constituents, later, the nematodes were treated with 1 mg/ml of I. paraguariensis from L1-larvae-stage until adulthood. The extract concentration was chosen because exhibited antioxidant proprieties against juglone, a generator of reactive oxygen species, since these are elevated in fat tissue of obese people. Lipid accumulation, total body energy expenditure and behavioral parameters were analyzed. Ilex paraguariensis decreased BODIPY labeling by 63.36% compared to control without affecting behaviors related to energetic balance. Total body energy expenditure of N2 wild-type, nhr-49 and ador-1 knockout strains was performed through oxygen consumption, Ilex paraguariensis was able to increase oxygen consumption only in N2 worms. In conclusion, Ilex paraguariensis decreased fat deposits and increased consumption of oxygen in N2 worms dependent on NHR-49 and ADOR-1 pathways. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-02-25 |
dc.date.accessioned.fl_str_mv |
2023-03-23T15:14:09Z |
dc.date.available.fl_str_mv |
2023-03-23T15:14:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/28350 |
url |
http://repositorio.ufsm.br/handle/1/28350 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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200800100009 |
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600 600 600 600 600 |
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3aef53b7-b173-4c56-a944-bed2de5e5dda 121a3386-f146-43dc-9b04-c78a9b47d7d4 ae9ccccd-6c23-46a1-abda-fa450ef31e4f 75ee513b-7658-4252-ad23-0af0d13b7c8e |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Bioquímica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
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