Avaliação da atividade fotodinâmica frente a agentes de micoses cutâneas e subcutâneas
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/20844 |
Resumo: | Cutaneous and subcutaneous mycoses affect both immunocompeten individuals and immunocompromised individuals. Generally prolonged therapeutic treatments and, recurrences during treatment are quite common. Scopulariopsis brevicaulis is an agent of cutaneous mycoses, mainly onychomycosis. Fonsecaea pedrosoi and Scedosporium apiospermum are agents of chromoblastomycosis and mycetomas, respectively. These fungal agents are resistant to most of the conventional antifungal agents used in the treatments. Photodynamic therapy emerges as an alternative to the treatment of these localized skin infections, combining three factors: photosensitizer, visible light and oxygen. Photosensitizer activation results in the production of reactive oxygen species that in a sequence of photochemical and photobiological reactions generates the inactivation of the target cell. In this study the photodynamic activity on S. brevicaulis, F. pedrosoi and S. apiospermum was evaluated, using phenothiazine photosensitizers and a diode laser (InGaAlP). The results demonstrated that the photodynamic activity was evidenced against S. brevicaulis and S. apiospermum, when methylene blue was used as a photosensitizer. On the other hand, when toluidine blue was used as a photosensitizer, the three fungal agents tested showed sensitivity to photodynamic activity. Methylene blue and toluidine blue are chemically dye of the phenothiazines group, and function as cationic phenothiazine photosensitizers, being very efficient in the production of singlet oxygen. In addition, they have a good interaction with the wall and the fungal plasma membrane. The results of this study suggest that photodynamic therapy may be a very promising treatment alternative, however, clinical studies carefully conducted need to be developed. |
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2021-05-12T11:39:51Z2021-05-12T11:39:51Z2018-08-24http://repositorio.ufsm.br/handle/1/20844Cutaneous and subcutaneous mycoses affect both immunocompeten individuals and immunocompromised individuals. Generally prolonged therapeutic treatments and, recurrences during treatment are quite common. Scopulariopsis brevicaulis is an agent of cutaneous mycoses, mainly onychomycosis. Fonsecaea pedrosoi and Scedosporium apiospermum are agents of chromoblastomycosis and mycetomas, respectively. These fungal agents are resistant to most of the conventional antifungal agents used in the treatments. Photodynamic therapy emerges as an alternative to the treatment of these localized skin infections, combining three factors: photosensitizer, visible light and oxygen. Photosensitizer activation results in the production of reactive oxygen species that in a sequence of photochemical and photobiological reactions generates the inactivation of the target cell. In this study the photodynamic activity on S. brevicaulis, F. pedrosoi and S. apiospermum was evaluated, using phenothiazine photosensitizers and a diode laser (InGaAlP). The results demonstrated that the photodynamic activity was evidenced against S. brevicaulis and S. apiospermum, when methylene blue was used as a photosensitizer. On the other hand, when toluidine blue was used as a photosensitizer, the three fungal agents tested showed sensitivity to photodynamic activity. Methylene blue and toluidine blue are chemically dye of the phenothiazines group, and function as cationic phenothiazine photosensitizers, being very efficient in the production of singlet oxygen. In addition, they have a good interaction with the wall and the fungal plasma membrane. The results of this study suggest that photodynamic therapy may be a very promising treatment alternative, however, clinical studies carefully conducted need to be developed.As micoses cutâneas e subcutâneas acometem tanto indivíduos imunocompetentes como indivíduos imunocomprometidos. Geralmente os tratamentos terapêuticos prolongados e as recorrências durante o tratamento são bastante comuns. Scopulariopsis brevicaulis é agente de micoses cutâneas, sobretudo de onicomicose. Fonsecaea pedrosoi e Scedosporium apiospermum são agentes de cromoblastomicose e micetomas, respectivamente. Esses agentes fúngicos mencionados são resistentes à maioria dos antifúngicos convencionais utilizados nos tratamentos. A terapia fotodinâmica então surge como uma alternativa ao tratamento dessas infecções localizadas de pele, combinando três fatores: fotossensibilizador, luz visível e oxigênio. A ativação do fotossensibilizador resulta na produção de espécies reativas de oxigênio que em uma sequência de reações fotoquímicas e fotobiológicas provocam a inativação da célula-alvo. Neste estudo foi avaliada a atividade fotodinâmica sobre S. brevicaulis, F. pedrosoi e S. apiospermum, utilizando-se fotossensibilizadores fenotiazínicos e um laser de diodo (InGaAlP). Os resultados demonstraram que a atividade fotodinâmica foi evidenciada frente a S. brevicaulis e S. apiospermum, quando se empregou o azul de metileno como fotossensibilizador. Por outro lado, quando se utilizou azul de toluidina como fotossensibilizador, os três agentes fúngicos testados evidenciaram sensibilidade à atividade fotodinâmica. O azul de metileno e o azul de toluidina são corantes quimicamente do grupo dos fenotiazínicos, e que funcionam como fotossensibilizadores fenotiazínicos catiônicos, sendo muito eficientes na produção de oxigênio singlete. Além disso, possuem uma ótima interação com a parede e a membrana plasmática fúngica. Os resultados desse estudo sugerem que a terapia fotodinâmica poderá se constituir numa alternativa de tratamento bastante promissora, todavia, estudos clínicos cuidadosamente conduzidos precisam ser desenvolvidos.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências da SaúdePrograma de Pós-Graduação em Ciências FarmacêuticasUFSMBrasilAnálises Clínicas e ToxicológicasAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessMicosesFungos filamentososResistênciaTerapia fotodinâmicaMycosesFilamentous fungiResistancePhotodynamic therapyCNPQ::CIENCIAS DA SAUDE::FARMACIAAvaliação da atividade fotodinâmica frente a agentes de micoses cutâneas e subcutâneasEvaluation of photodynamic activity against cutaneous and subcutaneous mycosis agentsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisAlves, Sydney Hartzhttp://lattes.cnpq.br/0330782478769631Schaffer, Larissa FingerSaraçol, Débora Nunes Mariohttp://lattes.cnpq.br/0902366317883514Keller, Jéssica Tairine400300000005600600600600600bfe6016c-9c1e-42ca-ab1d-db5453ed090cbd3e07a6-8b43-418f-bad3-9ce113c52d3d611a3ac4-4589-4e16-8d0e-fb476eaf0ede69df5795-e416-4246-9af6-2f6eac2fe203reponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Avaliação da atividade fotodinâmica frente a agentes de micoses cutâneas e subcutâneas |
dc.title.alternative.eng.fl_str_mv |
Evaluation of photodynamic activity against cutaneous and subcutaneous mycosis agents |
title |
Avaliação da atividade fotodinâmica frente a agentes de micoses cutâneas e subcutâneas |
spellingShingle |
Avaliação da atividade fotodinâmica frente a agentes de micoses cutâneas e subcutâneas Keller, Jéssica Tairine Micoses Fungos filamentosos Resistência Terapia fotodinâmica Mycoses Filamentous fungi Resistance Photodynamic therapy CNPQ::CIENCIAS DA SAUDE::FARMACIA |
title_short |
Avaliação da atividade fotodinâmica frente a agentes de micoses cutâneas e subcutâneas |
title_full |
Avaliação da atividade fotodinâmica frente a agentes de micoses cutâneas e subcutâneas |
title_fullStr |
Avaliação da atividade fotodinâmica frente a agentes de micoses cutâneas e subcutâneas |
title_full_unstemmed |
Avaliação da atividade fotodinâmica frente a agentes de micoses cutâneas e subcutâneas |
title_sort |
Avaliação da atividade fotodinâmica frente a agentes de micoses cutâneas e subcutâneas |
author |
Keller, Jéssica Tairine |
author_facet |
Keller, Jéssica Tairine |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Alves, Sydney Hartz |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0330782478769631 |
dc.contributor.referee1.fl_str_mv |
Schaffer, Larissa Finger |
dc.contributor.referee2.fl_str_mv |
Saraçol, Débora Nunes Mario |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0902366317883514 |
dc.contributor.author.fl_str_mv |
Keller, Jéssica Tairine |
contributor_str_mv |
Alves, Sydney Hartz Schaffer, Larissa Finger Saraçol, Débora Nunes Mario |
dc.subject.por.fl_str_mv |
Micoses Fungos filamentosos Resistência Terapia fotodinâmica |
topic |
Micoses Fungos filamentosos Resistência Terapia fotodinâmica Mycoses Filamentous fungi Resistance Photodynamic therapy CNPQ::CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
Mycoses Filamentous fungi Resistance Photodynamic therapy |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::FARMACIA |
description |
Cutaneous and subcutaneous mycoses affect both immunocompeten individuals and immunocompromised individuals. Generally prolonged therapeutic treatments and, recurrences during treatment are quite common. Scopulariopsis brevicaulis is an agent of cutaneous mycoses, mainly onychomycosis. Fonsecaea pedrosoi and Scedosporium apiospermum are agents of chromoblastomycosis and mycetomas, respectively. These fungal agents are resistant to most of the conventional antifungal agents used in the treatments. Photodynamic therapy emerges as an alternative to the treatment of these localized skin infections, combining three factors: photosensitizer, visible light and oxygen. Photosensitizer activation results in the production of reactive oxygen species that in a sequence of photochemical and photobiological reactions generates the inactivation of the target cell. In this study the photodynamic activity on S. brevicaulis, F. pedrosoi and S. apiospermum was evaluated, using phenothiazine photosensitizers and a diode laser (InGaAlP). The results demonstrated that the photodynamic activity was evidenced against S. brevicaulis and S. apiospermum, when methylene blue was used as a photosensitizer. On the other hand, when toluidine blue was used as a photosensitizer, the three fungal agents tested showed sensitivity to photodynamic activity. Methylene blue and toluidine blue are chemically dye of the phenothiazines group, and function as cationic phenothiazine photosensitizers, being very efficient in the production of singlet oxygen. In addition, they have a good interaction with the wall and the fungal plasma membrane. The results of this study suggest that photodynamic therapy may be a very promising treatment alternative, however, clinical studies carefully conducted need to be developed. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-08-24 |
dc.date.accessioned.fl_str_mv |
2021-05-12T11:39:51Z |
dc.date.available.fl_str_mv |
2021-05-12T11:39:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/20844 |
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http://repositorio.ufsm.br/handle/1/20844 |
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por |
language |
por |
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400300000005 |
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600 600 600 600 600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências da Saúde |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Farmacêuticas |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Análises Clínicas e Toxicológicas |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências da Saúde |
dc.source.none.fl_str_mv |
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