Atividade da adenosina desaminase no soro e nos linfócitos de ratos infectados por Sporothrix schenckii
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/10109 |
Resumo: | Sporotrichosis is a subcutaneous fungal infection of evolution subacute or chronic, inflammatory lesions characterized by pyogranulomatous aspect, caused by the dimorphic fungus Sporothrix schenckii. Adenosine deaminase (ADA) is a key enzyme in the purine metabolism, promoting the deamination of adenosine, an important anti-inflammatory molecule. The increase in ADA activity has been demonstrated in several inflammatory conditions, however, no data in the literature associated with this fungal infection. The objective of this study was to evaluate the activity of serum ADA (S-ADA) and lymphocytes (L-ADA) of rats infected with S. schenckii. We used seventy-eight rats divided into two groups. In the first experiment, rats were infected subcutaneously and in the second experiment, infected intraperitoneally. Blood samples for hematologic evaluation and activities of S-ADA and ADA-L were performed on days 15, 30 and 40 post-infection (PI) to assess disease progression. In experiment II, was observed in an acute decrease in activity of S-ADA and L-ADA (p <0.05), suggesting a compensatory mechanism in the body's attempt to protect the host from excessive tissue damage. Chronicity of the disease the rats in the experiment I and II at 30 days PI, showed an increased activity of L-ADA (p <0.05), promoting an inflammatory response in an attempt to combat the spread of the agent. Thus, it is suggested that infection with S. schenckii alters the activities of S-ADA experimentally infected rats, demonstrating the involvement of this enzyme in the pathogenesis of sporotrichosis. |
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2012-08-302012-08-302011-10-03CASTRO, Verônica Souza Paiva. ADENOSINE DEAMINASE ACTIVITY IN SERUM AND LYMPHOCYTES OF RATS INFECTED BY Sporothrix schenckii. 2011. 48 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2011.http://repositorio.ufsm.br/handle/1/10109Sporotrichosis is a subcutaneous fungal infection of evolution subacute or chronic, inflammatory lesions characterized by pyogranulomatous aspect, caused by the dimorphic fungus Sporothrix schenckii. Adenosine deaminase (ADA) is a key enzyme in the purine metabolism, promoting the deamination of adenosine, an important anti-inflammatory molecule. The increase in ADA activity has been demonstrated in several inflammatory conditions, however, no data in the literature associated with this fungal infection. The objective of this study was to evaluate the activity of serum ADA (S-ADA) and lymphocytes (L-ADA) of rats infected with S. schenckii. We used seventy-eight rats divided into two groups. In the first experiment, rats were infected subcutaneously and in the second experiment, infected intraperitoneally. Blood samples for hematologic evaluation and activities of S-ADA and ADA-L were performed on days 15, 30 and 40 post-infection (PI) to assess disease progression. In experiment II, was observed in an acute decrease in activity of S-ADA and L-ADA (p <0.05), suggesting a compensatory mechanism in the body's attempt to protect the host from excessive tissue damage. Chronicity of the disease the rats in the experiment I and II at 30 days PI, showed an increased activity of L-ADA (p <0.05), promoting an inflammatory response in an attempt to combat the spread of the agent. Thus, it is suggested that infection with S. schenckii alters the activities of S-ADA experimentally infected rats, demonstrating the involvement of this enzyme in the pathogenesis of sporotrichosis.A esporotricose é uma infecção micótica subcutânea de evolução subaguda ou crônica, caracterizada por lesões inflamatórias de aspecto piogranulomatoso, causada pelo fungo dimórfico Sporothrix schenckii. A adenosina desaminase (ADA) é uma enzima chave no metabolismo das purinas, promovendo a desaminação da adenosina uma importante molécula anti-inflamatória. O aumento na atividade da ADA tem sido demonstrado em várias condições inflamatórias, porém, não existem dados na literatura associados com esta infecção micótica. O objetivo deste estudo foi avaliar a atividade da ADA no soro (S-ADA) e nos linfócitos (L-ADA) de ratos infectados por S. schenckii. Foram utilizados setenta e oito ratos distribuídos em dois grupos. No experimento I, os ratos foram infectados por via subcutânea e no experimento II, infectados por via intraperitoneal. A coleta de sangue para a avaliação hematológica e atividades da S-ADA e L-ADA foram realizadas nos dias 15, 30 e 40 pós-infecção (PI), para avaliar a evolução da doença. No experimento II, foi observada na fase aguda uma diminuição na atividade da S-ADA e L-ADA (p<0.05), sugerindo um mecanismo compensatório do organismo na tentativa de proteger o hospedeiro da lesão tecidual excessiva. Com a cronicidade da enfermidade os ratos do experimento I e II aos 30 dias PI, apresentaram um aumento na atividade da L-ADA (p<0.05), promovendo uma resposta inflamatória na tentativa de combater a proliferação do agente. Assim, sugere-se que a infecção pelo S. schenckii altera as atividades da S-ADA e L-ADA de ratos infectados experimentalmente, demonstrando o envolvimento desta enzima na patogênese da esporotricose.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Medicina VeterináriaUFSMBRMedicina VeterináriaEsporotricoseAdenosina desaminaseLinfócitosSoroSporotrichosisAdenosine deaminaseLymphocytesSerumCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAAtividade da adenosina desaminase no soro e nos linfócitos de ratos infectados por Sporothrix schenckiiAdenosine deaminase activity in serum and lymphocytes of rats infected by Sporothrix schenckiiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisMazzanti, Cinthia Melazzo de Andradehttp://lattes.cnpq.br/2886709251370905Spanevello, Roselia Mariahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4702772A1Lopes, Sonia Teresinha dos Anjoshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794864D7http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4219288H6Castro, Verônica Souza Paiva5005000000074005005005005007b1818e4-b459-4c88-84ca-e98b413246dcef665b2b-43fa-44fa-a613-0541eb6991d72c5b3e7b-5bee-4ab4-92a9-97c6a36565e3aac588d9-5a32-46a9-b6f9-2f297a059022info:eu-repo/semantics/openAccessreponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALCASTRO, VERONICA SOUZA PAIVA.pdfapplication/pdf1006741http://repositorio.ufsm.br/bitstream/1/10109/1/CASTRO%2c%20VERONICA%20SOUZA%20PAIVA.pdf53a05d776fa42214b1c4ef0270f4e0abMD51TEXTCASTRO, VERONICA SOUZA PAIVA.pdf.txtCASTRO, VERONICA SOUZA PAIVA.pdf.txtExtracted texttext/plain74566http://repositorio.ufsm.br/bitstream/1/10109/2/CASTRO%2c%20VERONICA%20SOUZA%20PAIVA.pdf.txt7ff17b75dc597a74850046d850414398MD52THUMBNAILCASTRO, VERONICA SOUZA PAIVA.pdf.jpgCASTRO, VERONICA SOUZA PAIVA.pdf.jpgIM Thumbnailimage/jpeg4959http://repositorio.ufsm.br/bitstream/1/10109/3/CASTRO%2c%20VERONICA%20SOUZA%20PAIVA.pdf.jpg2509ec460225fa632d877c38476d9159MD531/101092022-09-02 10:07:46.568oai:repositorio.ufsm.br:1/10109Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestouvidoria@ufsm.bropendoar:39132022-09-02T13:07:46Repositório Institucional Manancial UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Atividade da adenosina desaminase no soro e nos linfócitos de ratos infectados por Sporothrix schenckii |
dc.title.alternative.eng.fl_str_mv |
Adenosine deaminase activity in serum and lymphocytes of rats infected by Sporothrix schenckii |
title |
Atividade da adenosina desaminase no soro e nos linfócitos de ratos infectados por Sporothrix schenckii |
spellingShingle |
Atividade da adenosina desaminase no soro e nos linfócitos de ratos infectados por Sporothrix schenckii Castro, Verônica Souza Paiva Esporotricose Adenosina desaminase Linfócitos Soro Sporotrichosis Adenosine deaminase Lymphocytes Serum CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Atividade da adenosina desaminase no soro e nos linfócitos de ratos infectados por Sporothrix schenckii |
title_full |
Atividade da adenosina desaminase no soro e nos linfócitos de ratos infectados por Sporothrix schenckii |
title_fullStr |
Atividade da adenosina desaminase no soro e nos linfócitos de ratos infectados por Sporothrix schenckii |
title_full_unstemmed |
Atividade da adenosina desaminase no soro e nos linfócitos de ratos infectados por Sporothrix schenckii |
title_sort |
Atividade da adenosina desaminase no soro e nos linfócitos de ratos infectados por Sporothrix schenckii |
author |
Castro, Verônica Souza Paiva |
author_facet |
Castro, Verônica Souza Paiva |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Mazzanti, Cinthia Melazzo de Andrade |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2886709251370905 |
dc.contributor.referee1.fl_str_mv |
Spanevello, Roselia Maria |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4702772A1 |
dc.contributor.referee2.fl_str_mv |
Lopes, Sonia Teresinha dos Anjos |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794864D7 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4219288H6 |
dc.contributor.author.fl_str_mv |
Castro, Verônica Souza Paiva |
contributor_str_mv |
Mazzanti, Cinthia Melazzo de Andrade Spanevello, Roselia Maria Lopes, Sonia Teresinha dos Anjos |
dc.subject.por.fl_str_mv |
Esporotricose Adenosina desaminase Linfócitos Soro |
topic |
Esporotricose Adenosina desaminase Linfócitos Soro Sporotrichosis Adenosine deaminase Lymphocytes Serum CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Sporotrichosis Adenosine deaminase Lymphocytes Serum |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Sporotrichosis is a subcutaneous fungal infection of evolution subacute or chronic, inflammatory lesions characterized by pyogranulomatous aspect, caused by the dimorphic fungus Sporothrix schenckii. Adenosine deaminase (ADA) is a key enzyme in the purine metabolism, promoting the deamination of adenosine, an important anti-inflammatory molecule. The increase in ADA activity has been demonstrated in several inflammatory conditions, however, no data in the literature associated with this fungal infection. The objective of this study was to evaluate the activity of serum ADA (S-ADA) and lymphocytes (L-ADA) of rats infected with S. schenckii. We used seventy-eight rats divided into two groups. In the first experiment, rats were infected subcutaneously and in the second experiment, infected intraperitoneally. Blood samples for hematologic evaluation and activities of S-ADA and ADA-L were performed on days 15, 30 and 40 post-infection (PI) to assess disease progression. In experiment II, was observed in an acute decrease in activity of S-ADA and L-ADA (p <0.05), suggesting a compensatory mechanism in the body's attempt to protect the host from excessive tissue damage. Chronicity of the disease the rats in the experiment I and II at 30 days PI, showed an increased activity of L-ADA (p <0.05), promoting an inflammatory response in an attempt to combat the spread of the agent. Thus, it is suggested that infection with S. schenckii alters the activities of S-ADA experimentally infected rats, demonstrating the involvement of this enzyme in the pathogenesis of sporotrichosis. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-10-03 |
dc.date.accessioned.fl_str_mv |
2012-08-30 |
dc.date.available.fl_str_mv |
2012-08-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
CASTRO, Verônica Souza Paiva. ADENOSINE DEAMINASE ACTIVITY IN SERUM AND LYMPHOCYTES OF RATS INFECTED BY Sporothrix schenckii. 2011. 48 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/10109 |
identifier_str_mv |
CASTRO, Verônica Souza Paiva. ADENOSINE DEAMINASE ACTIVITY IN SERUM AND LYMPHOCYTES OF RATS INFECTED BY Sporothrix schenckii. 2011. 48 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2011. |
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http://repositorio.ufsm.br/handle/1/10109 |
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