Testes diagnósticos para detecção de bovinos infectados por Mycobacterium bovis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/21607 |
Resumo: | Bovine tuberculosis remains a global health problem, both for developing countries, as for the developed countries. The presence of the bacterium Mycobacterium bovis represents a hazard to human and cattle population and its detection depends on diagnostic tools, which are still imperfect. This thesis describes part of the problem of the performance evaluation of two indirect enzyme-linked immunosorbent assays (ELISAs), one in experimental stage and the other a commercial kit to detect antibody of cattle naturally infected by M. bovis, when other diagnostic techniques also fail to hit the result. We also show the problem faced by the threshold of the cervical comparative tuberculin (CCT) test, which is the method most widely used for the control and eradication of bovine tuberculosis in field use. Twenty-one herds of cattle, 19 of dairy production and two predominantly for beef production, have been tested with the CCT and the ELISAs’ results have been compared, using 2703 serum samples collected simultaneously. The experimental ELISA uses a chimera formed of three proteins fragments of M. bovis, MPB-70, MPB-83 and ESAT-6 as antigen, and the commercial ELISA uses the MPB-70 and MPB-83. The experimental ELISA detected all 10 CCT positive herds and the commercial ELISA has detect nine of them. The experimental ELISA also detected more cattle as positive. In the second article, the CCT, which is the reference test for bovine tuberculosis control, was analyzed in a herd, when the skin-fold increase produced false-negative, in detriment of the decision making when the cut-off point is taken to the letter. The false-negative results must be considered both in controlling the disease in farms infected by M. bovis, as in surveillance studies using this test. |
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2021-07-27T16:13:24Z2021-07-27T16:13:24Z2013-05-07http://repositorio.ufsm.br/handle/1/21607Bovine tuberculosis remains a global health problem, both for developing countries, as for the developed countries. The presence of the bacterium Mycobacterium bovis represents a hazard to human and cattle population and its detection depends on diagnostic tools, which are still imperfect. This thesis describes part of the problem of the performance evaluation of two indirect enzyme-linked immunosorbent assays (ELISAs), one in experimental stage and the other a commercial kit to detect antibody of cattle naturally infected by M. bovis, when other diagnostic techniques also fail to hit the result. We also show the problem faced by the threshold of the cervical comparative tuberculin (CCT) test, which is the method most widely used for the control and eradication of bovine tuberculosis in field use. Twenty-one herds of cattle, 19 of dairy production and two predominantly for beef production, have been tested with the CCT and the ELISAs’ results have been compared, using 2703 serum samples collected simultaneously. The experimental ELISA uses a chimera formed of three proteins fragments of M. bovis, MPB-70, MPB-83 and ESAT-6 as antigen, and the commercial ELISA uses the MPB-70 and MPB-83. The experimental ELISA detected all 10 CCT positive herds and the commercial ELISA has detect nine of them. The experimental ELISA also detected more cattle as positive. In the second article, the CCT, which is the reference test for bovine tuberculosis control, was analyzed in a herd, when the skin-fold increase produced false-negative, in detriment of the decision making when the cut-off point is taken to the letter. The false-negative results must be considered both in controlling the disease in farms infected by M. bovis, as in surveillance studies using this test.A tuberculose bovina permanece sendo um problema sanitário mundial, tanto aos países em desenvolvimento, quanto aos países já desenvolvidos. A presença da bactéria Mycobacterium bovis representa perigo à população bovina e humana e a sua detecção depende de ferramentas diagnósticas, que ainda são imperfeitas. O presente trabalho descreve parte do problema da avaliação do desempenho de dois testes imunoenzimáticos ELISA, um em fase experimental e outro já em estádio comercial para a detecção de anticorpos de bovinos naturalmente infectados por M. bovis, quando outras técnicas diagnósticas também falham em acertar no resultado. Mostra também o problema enfrentado pelo limiar definido nacionalmente para o teste cervical comparativo (TCC), que é o método mais amplamente utilizado no controle e erradicação da tuberculose bovina em uso no campo. Vinte e um rebanhos de bovinos, 19 dos quais de produção de leite e dois predominantemente de corte, tiveram testes de tuberculinização comparados aos testes ELISA, aplicados em 2703 amostras de soro, colhidas simultaneamente. O ELISA experimental utiliza como antígeno uma quimera formada por fragmentos de três proteínas de M. bovis, MPB-70, MPB-83 e ESAT-6, e o ELISA comercial, a MPB-70 e a MPB-83. O ELISA experimental detectou todos os dez rebanhos e o ELISA comercial nove dos rebanhos positivos pela tuberculinização. O ELISA experimental também detectou mais bovinos como positivos. No segundo artigo, o teste cervical comparativo, que é o teste de referência para o controle da tuberculose, foi analisado em um dos rebanhos, quando o aumento da prega da pele produziu falsos-negativos, prejudicando a tomada de decisão frente ao “ponto-de-corte”. Os falsos-negativos devem ser considerados tanto no controle da doença em criatórios com infecção por M. bovis, quanto nos estudos de prevalência, que estão utilizando esse teste.porUniversidade Federal de Santa MariaCentro de Ciências RuraisPrograma de Pós-Graduação em Medicina VeterináriaUFSMBrasilMedicina VeterináriaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessTuberculose bovinaDiagnósticoELISATeste intradérmicoControleBovine tuberculosisDiagnosisSkin testDisease controlCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIATestes diagnósticos para detecção de bovinos infectados por Mycobacterium bovisDiagnostic tests to detect cattle infected by Mycobacterium bovisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisVargas, Agueda Palmira Castagna dehttp://lattes.cnpq.br/1383126157031968Roxo, ElianaRibeiro, Anna Monteiro Correia LimaVogel, Fernanda Silveira FloresWeiblen, Rudihttp://lattes.cnpq.br/1534404061779629Vidal, Carlos Eugênio Soto5005000000076006006006006006006007561081c-bfab-4ea7-ace8-2bf6488cb0b5f162d4a8-7a1a-4996-8e25-ed99cf86783f20e70898-7a70-4492-bd6a-73a3d447119e9cdda48b-a8c3-4d79-a9b0-b3a81bae1a7241cb1c92-0393-40eb-94c6-4128074bd1a531cd7453-8e16-4d9b-a7c9-50aebad0ba0ereponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALTES_PPGMV_2013_VIDAL_CARLOS.pdfTES_PPGMV_2013_VIDAL_CARLOS.pdfTeseapplication/pdf3011109http://repositorio.ufsm.br/bitstream/1/21607/1/TES_PPGMV_2013_VIDAL_CARLOS.pdfe5b526e6364188fb0982f984b43cf25cMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Testes diagnósticos para detecção de bovinos infectados por Mycobacterium bovis |
dc.title.alternative.eng.fl_str_mv |
Diagnostic tests to detect cattle infected by Mycobacterium bovis |
title |
Testes diagnósticos para detecção de bovinos infectados por Mycobacterium bovis |
spellingShingle |
Testes diagnósticos para detecção de bovinos infectados por Mycobacterium bovis Vidal, Carlos Eugênio Soto Tuberculose bovina Diagnóstico ELISA Teste intradérmico Controle Bovine tuberculosis Diagnosis Skin test Disease control CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Testes diagnósticos para detecção de bovinos infectados por Mycobacterium bovis |
title_full |
Testes diagnósticos para detecção de bovinos infectados por Mycobacterium bovis |
title_fullStr |
Testes diagnósticos para detecção de bovinos infectados por Mycobacterium bovis |
title_full_unstemmed |
Testes diagnósticos para detecção de bovinos infectados por Mycobacterium bovis |
title_sort |
Testes diagnósticos para detecção de bovinos infectados por Mycobacterium bovis |
author |
Vidal, Carlos Eugênio Soto |
author_facet |
Vidal, Carlos Eugênio Soto |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Vargas, Agueda Palmira Castagna de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1383126157031968 |
dc.contributor.referee1.fl_str_mv |
Roxo, Eliana |
dc.contributor.referee2.fl_str_mv |
Ribeiro, Anna Monteiro Correia Lima |
dc.contributor.referee3.fl_str_mv |
Vogel, Fernanda Silveira Flores |
dc.contributor.referee4.fl_str_mv |
Weiblen, Rudi |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1534404061779629 |
dc.contributor.author.fl_str_mv |
Vidal, Carlos Eugênio Soto |
contributor_str_mv |
Vargas, Agueda Palmira Castagna de Roxo, Eliana Ribeiro, Anna Monteiro Correia Lima Vogel, Fernanda Silveira Flores Weiblen, Rudi |
dc.subject.por.fl_str_mv |
Tuberculose bovina Diagnóstico ELISA Teste intradérmico Controle |
topic |
Tuberculose bovina Diagnóstico ELISA Teste intradérmico Controle Bovine tuberculosis Diagnosis Skin test Disease control CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Bovine tuberculosis Diagnosis Skin test Disease control |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Bovine tuberculosis remains a global health problem, both for developing countries, as for the developed countries. The presence of the bacterium Mycobacterium bovis represents a hazard to human and cattle population and its detection depends on diagnostic tools, which are still imperfect. This thesis describes part of the problem of the performance evaluation of two indirect enzyme-linked immunosorbent assays (ELISAs), one in experimental stage and the other a commercial kit to detect antibody of cattle naturally infected by M. bovis, when other diagnostic techniques also fail to hit the result. We also show the problem faced by the threshold of the cervical comparative tuberculin (CCT) test, which is the method most widely used for the control and eradication of bovine tuberculosis in field use. Twenty-one herds of cattle, 19 of dairy production and two predominantly for beef production, have been tested with the CCT and the ELISAs’ results have been compared, using 2703 serum samples collected simultaneously. The experimental ELISA uses a chimera formed of three proteins fragments of M. bovis, MPB-70, MPB-83 and ESAT-6 as antigen, and the commercial ELISA uses the MPB-70 and MPB-83. The experimental ELISA detected all 10 CCT positive herds and the commercial ELISA has detect nine of them. The experimental ELISA also detected more cattle as positive. In the second article, the CCT, which is the reference test for bovine tuberculosis control, was analyzed in a herd, when the skin-fold increase produced false-negative, in detriment of the decision making when the cut-off point is taken to the letter. The false-negative results must be considered both in controlling the disease in farms infected by M. bovis, as in surveillance studies using this test. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-05-07 |
dc.date.accessioned.fl_str_mv |
2021-07-27T16:13:24Z |
dc.date.available.fl_str_mv |
2021-07-27T16:13:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/21607 |
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http://repositorio.ufsm.br/handle/1/21607 |
dc.language.iso.fl_str_mv |
por |
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por |
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500500000007 |
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600 600 600 600 600 600 600 |
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dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Medicina Veterinária |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Medicina Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.source.none.fl_str_mv |
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