Comparison of seminal characteristics of Aberdeen Angus, Holstein and Nelore bulls before and after cryopreservation

Detalhes bibliográficos
Autor(a) principal: Saranholi, Diego André Costa
Data de Publicação: 2021
Outros Autores: Paula, Rafael Rocha de, Pytilak, Edmilson, Afonso, Fabíola, Canela, Luis Felipe, Almeida, Ana Beatriz Marques de, Hidalgo, Myrian Megumy Tsunokawa, Martins, Maria Isabel Mello, Blaschi, Wanessa, Barreiros, Thales Ricardo Rigo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/23382
Resumo: The aim of this study was to evaluate parameters indicative of sperm quality of fresh and post-thawed semen of Aberdeen Angus, Holstein and Nelore bulls. Thirty-nine bulls were used: Aberdeen Angus (n=13), Holstein (n=13) and Nelore (n=13). The ejaculate collects were performed twice a week using artificial vagina, totaling 792 semen collections, 307 for Aberdeen Angus, 225 for Holstein and 260 for Nelore bulls. After collection, fresh semen was evaluated and semen freezing was performed. After freezing, the batches were thawed and progressive motility was determined. The analysis of fresh semen showed that there was no difference (P = 0.053) between the Aberdeen Angus and Nelore breeds, while ejaculates from Holstein bulls showed a statistical difference (P = 0.024). As well, a difference (P<0.001) was identified in the sperm concentration of the three breeds. In the samples evaluated after thawing, a statistical difference was observed between Holstein and Nelore breeds (P<0.001), while the values of the Angus breed were similar to the other two breeds. The difference in motility of fresh and post-thawing semen showed that Nelore and Angus bulls showed greater variation in values between the analyzes (26.0±8.9% and 25.3±8.4%, respectively) showing a significant difference (P<0.001) in relation to Holstein bulls (20.6±9.3%) that obtained the smallest difference. The analysis of fresh and post-thawing semen did not show any significant difference (P=0.13) between breeds. In conclusion, the semen cryopreservation process causes a decrease in the physical parameters of the semen and these quality losses suffer interference according to the breeds.
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spelling Comparison of seminal characteristics of Aberdeen Angus, Holstein and Nelore bulls before and after cryopreservationComparación de las características seminales de toros Aberdeen angus, Holandés y Nelore antes y después de la criopreservaciónComparação das características seminais de touros das raças Aberdeen Angus, Holandês e Nelore antes e após a criopreservaçãoFreezingBovineBos taurusBos indicus.CongelaciónToroBos taurusBos indicus.CongelaçãoBovinoBos taurusBos indicus.The aim of this study was to evaluate parameters indicative of sperm quality of fresh and post-thawed semen of Aberdeen Angus, Holstein and Nelore bulls. Thirty-nine bulls were used: Aberdeen Angus (n=13), Holstein (n=13) and Nelore (n=13). The ejaculate collects were performed twice a week using artificial vagina, totaling 792 semen collections, 307 for Aberdeen Angus, 225 for Holstein and 260 for Nelore bulls. After collection, fresh semen was evaluated and semen freezing was performed. After freezing, the batches were thawed and progressive motility was determined. The analysis of fresh semen showed that there was no difference (P = 0.053) between the Aberdeen Angus and Nelore breeds, while ejaculates from Holstein bulls showed a statistical difference (P = 0.024). As well, a difference (P<0.001) was identified in the sperm concentration of the three breeds. In the samples evaluated after thawing, a statistical difference was observed between Holstein and Nelore breeds (P<0.001), while the values of the Angus breed were similar to the other two breeds. The difference in motility of fresh and post-thawing semen showed that Nelore and Angus bulls showed greater variation in values between the analyzes (26.0±8.9% and 25.3±8.4%, respectively) showing a significant difference (P<0.001) in relation to Holstein bulls (20.6±9.3%) that obtained the smallest difference. The analysis of fresh and post-thawing semen did not show any significant difference (P=0.13) between breeds. In conclusion, the semen cryopreservation process causes a decrease in the physical parameters of the semen and these quality losses suffer interference according to the breeds.El objetivo de este estudio fue evaluar los parámetros de calidad del esperma de semen fresco y después de la descongelación de toros Aberdeen Angus, Holandés y Nelore. Se utilizaron 39 toros: Aberdeen Angus (n = 13), Holandés (n = 13) y Nelore (n = 13). La recolección de semen se realizó por vagina artificial dos veces por semana, totalizando 792 recolecciones de semen, 307 para toros Aberdeen Angus, 225 para toros holandeses y 260 para toros Nelore. Después de la cosecha, se evaluó el semen fresco y luego se congeló. Después de la congelación, se descongelaron las dosis y se determinó la motilidad progresiva. El análisis de semen fresco mostró que no hubo diferencia (P = 0.053) entre las razas Aberdeen Angus y Nelore, mientras que los eyaculados de toros Holandés mostraron una diferencia (P = 0.024). Además, se identificó diferencia (P <0,001) en la concentración de esperma de las tres razas. En las muestras evaluadas después de la descongelación, hubo diferencia entre los toros Holandés y Nelore (P <0.001), mientras que los valores de los toros Angus fueron similares a las otras dos razas. La diferencia en la motilidad del semen fresco y post-descongelación mostró que los toros Nelore y Angus presentaron mayor variación en los valores entre los análisis (26,0 ± 8,9% y 25,3 ± 8,4%, respectivamente) mostrando una diferencia (P <0,001) en comparación a los toros holandeses (20,6 ± 9,3%) que tuvieron la menor diferencia. El análisis de semen fresco y posterior a la descongelación no mostró diferencias (P = 0,13) entre razas. En conclusión, el proceso de criopreservación de semen redujo los parámetros físicos del semen y estas pérdidas de calidad sufren interferencia según las razas evaluadas.O objetivo deste estudo foi avaliar parâmetros da qualidade espermática de sêmen fresco e após a descongelação de touros Aberdeen Angus, Holandês e Nelore. Foram utilizados 39 touros: Aberdeen Angus (n=13), Holandês (n=13) e Nelore (n=13). A colheita de sêmen foi realizada por vagina artificial duas vezes/semana, totalizando 792 colheitas de sêmen, sendo 307 para touros Aberdeen Angus, 225 para touros Holandês e 260 para touros Nelore. Após colheita, o sêmen fresco foi avaliado e em seguida realizada a congelação. Após a congelação, as partidas foram descongeladas e foi determinada a motilidade progressiva. A análise do sêmen fresco demonstrou que não houve diferença (P= 0,053) entre as raças Aberdeen Angus e Nelore, enquanto, que ejaculados de touros Holandês observou-se diferença estatística (P= 0,024). Assim como, foi identificado diferença (P<0,001) na concentração espermática das três raças. Nas amostras avaliadas pós-descongelação, observou-se diferença estatística entre touros Holandês e Nelore (P<0,001), enquanto, que os valores de touros Angus foram semelhantes às outras duas raças. A diferença da motilidade do sêmen fresco e pós- descongelação evidenciou que touros Nelore e Angus apresentaram maior variação dos valores entre as análises (26,0±8,9% e 25,3±8,4%, respectivamente) apresentando diferença significativa (P<0,001) em relação aos touros Holandês (20,6±9,3%) que obtiveram a menor diferença. A Análise do sêmen fresco e pós- descongelação não apresentou diferença significativa (P=0,13) entre as raças. Em conclusão, o processo de criopreservação do sêmen reduziu os parâmetros físicos do sêmen e estas perdas de qualidade sofrem interferência de acordo com as raças avaliadas.Research, Society and Development2021-12-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2338210.33448/rsd-v10i16.23382Research, Society and Development; Vol. 10 No. 16; e408101623382Research, Society and Development; Vol. 10 Núm. 16; e408101623382Research, Society and Development; v. 10 n. 16; e4081016233822525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/23382/21058Copyright (c) 2021 Diego André Costa Saranholi; Rafael Rocha de Paula; Edmilson Pytilak; Fabíola Afonso; Luis Felipe Canela; Ana Beatriz Marques de Almeida; Myrian Megumy Tsunokawa Hidalgo; Maria Isabel Mello Martins; Wanessa Blaschi; Thales Ricardo Rigo Barreiroshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSaranholi, Diego André CostaPaula, Rafael Rocha de Pytilak, Edmilson Afonso, FabíolaCanela, Luis FelipeAlmeida, Ana Beatriz Marques deHidalgo, Myrian Megumy TsunokawaMartins, Maria Isabel MelloBlaschi, WanessaBarreiros, Thales Ricardo Rigo 2021-12-20T11:03:07Zoai:ojs.pkp.sfu.ca:article/23382Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:42:16.395250Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Comparison of seminal characteristics of Aberdeen Angus, Holstein and Nelore bulls before and after cryopreservation
Comparación de las características seminales de toros Aberdeen angus, Holandés y Nelore antes y después de la criopreservación
Comparação das características seminais de touros das raças Aberdeen Angus, Holandês e Nelore antes e após a criopreservação
title Comparison of seminal characteristics of Aberdeen Angus, Holstein and Nelore bulls before and after cryopreservation
spellingShingle Comparison of seminal characteristics of Aberdeen Angus, Holstein and Nelore bulls before and after cryopreservation
Saranholi, Diego André Costa
Freezing
Bovine
Bos taurus
Bos indicus.
Congelación
Toro
Bos taurus
Bos indicus.
Congelação
Bovino
Bos taurus
Bos indicus.
title_short Comparison of seminal characteristics of Aberdeen Angus, Holstein and Nelore bulls before and after cryopreservation
title_full Comparison of seminal characteristics of Aberdeen Angus, Holstein and Nelore bulls before and after cryopreservation
title_fullStr Comparison of seminal characteristics of Aberdeen Angus, Holstein and Nelore bulls before and after cryopreservation
title_full_unstemmed Comparison of seminal characteristics of Aberdeen Angus, Holstein and Nelore bulls before and after cryopreservation
title_sort Comparison of seminal characteristics of Aberdeen Angus, Holstein and Nelore bulls before and after cryopreservation
author Saranholi, Diego André Costa
author_facet Saranholi, Diego André Costa
Paula, Rafael Rocha de
Pytilak, Edmilson
Afonso, Fabíola
Canela, Luis Felipe
Almeida, Ana Beatriz Marques de
Hidalgo, Myrian Megumy Tsunokawa
Martins, Maria Isabel Mello
Blaschi, Wanessa
Barreiros, Thales Ricardo Rigo
author_role author
author2 Paula, Rafael Rocha de
Pytilak, Edmilson
Afonso, Fabíola
Canela, Luis Felipe
Almeida, Ana Beatriz Marques de
Hidalgo, Myrian Megumy Tsunokawa
Martins, Maria Isabel Mello
Blaschi, Wanessa
Barreiros, Thales Ricardo Rigo
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Saranholi, Diego André Costa
Paula, Rafael Rocha de
Pytilak, Edmilson
Afonso, Fabíola
Canela, Luis Felipe
Almeida, Ana Beatriz Marques de
Hidalgo, Myrian Megumy Tsunokawa
Martins, Maria Isabel Mello
Blaschi, Wanessa
Barreiros, Thales Ricardo Rigo
dc.subject.por.fl_str_mv Freezing
Bovine
Bos taurus
Bos indicus.
Congelación
Toro
Bos taurus
Bos indicus.
Congelação
Bovino
Bos taurus
Bos indicus.
topic Freezing
Bovine
Bos taurus
Bos indicus.
Congelación
Toro
Bos taurus
Bos indicus.
Congelação
Bovino
Bos taurus
Bos indicus.
description The aim of this study was to evaluate parameters indicative of sperm quality of fresh and post-thawed semen of Aberdeen Angus, Holstein and Nelore bulls. Thirty-nine bulls were used: Aberdeen Angus (n=13), Holstein (n=13) and Nelore (n=13). The ejaculate collects were performed twice a week using artificial vagina, totaling 792 semen collections, 307 for Aberdeen Angus, 225 for Holstein and 260 for Nelore bulls. After collection, fresh semen was evaluated and semen freezing was performed. After freezing, the batches were thawed and progressive motility was determined. The analysis of fresh semen showed that there was no difference (P = 0.053) between the Aberdeen Angus and Nelore breeds, while ejaculates from Holstein bulls showed a statistical difference (P = 0.024). As well, a difference (P<0.001) was identified in the sperm concentration of the three breeds. In the samples evaluated after thawing, a statistical difference was observed between Holstein and Nelore breeds (P<0.001), while the values of the Angus breed were similar to the other two breeds. The difference in motility of fresh and post-thawing semen showed that Nelore and Angus bulls showed greater variation in values between the analyzes (26.0±8.9% and 25.3±8.4%, respectively) showing a significant difference (P<0.001) in relation to Holstein bulls (20.6±9.3%) that obtained the smallest difference. The analysis of fresh and post-thawing semen did not show any significant difference (P=0.13) between breeds. In conclusion, the semen cryopreservation process causes a decrease in the physical parameters of the semen and these quality losses suffer interference according to the breeds.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/23382
10.33448/rsd-v10i16.23382
url https://rsdjournal.org/index.php/rsd/article/view/23382
identifier_str_mv 10.33448/rsd-v10i16.23382
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/23382/21058
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 16; e408101623382
Research, Society and Development; Vol. 10 Núm. 16; e408101623382
Research, Society and Development; v. 10 n. 16; e408101623382
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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