Protein and antigen profiles of Leptospira interrogans serovar Hardjo

Detalhes bibliográficos
Autor(a) principal: Lafetá,Bárbara Nobre
Data de Publicação: 2009
Outros Autores: Castro,Elaine Cristina de, Silva,Nivaldo da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000900024
Resumo: The protein profile of the outer membrane of Leptospira interrogans serovar Hardjo subtype hardjoprajitno associated with the bovine natural immune response was investigated. The outer membrane proteins were extracted utilizing Triton X114 and precipitated with acetone. The protein sample was then resolved by SDS-PAGE and reacted in western blot against sera from a hyperimmune rabbit and from naturally infected bovines. In silver stained gels, 14 protein bands were observed, among which four proteins, with 22, 29, 47 and 63kDa, appeared as major constituents. Western blot tests with hyperimmune rabbit antiserum detected bands corresponding to proteins with 35; 27; 24; 21; 17 and 14kDa, while 32kDa and 45kDa proteins were the most immunoreactive with sera from naturally infected bovines.
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spelling Protein and antigen profiles of Leptospira interrogans serovar HardjoLeptospira interrogansouter membrane proteinhardjoprajitnoimmunomodulatory proteinsThe protein profile of the outer membrane of Leptospira interrogans serovar Hardjo subtype hardjoprajitno associated with the bovine natural immune response was investigated. The outer membrane proteins were extracted utilizing Triton X114 and precipitated with acetone. The protein sample was then resolved by SDS-PAGE and reacted in western blot against sera from a hyperimmune rabbit and from naturally infected bovines. In silver stained gels, 14 protein bands were observed, among which four proteins, with 22, 29, 47 and 63kDa, appeared as major constituents. Western blot tests with hyperimmune rabbit antiserum detected bands corresponding to proteins with 35; 27; 24; 21; 17 and 14kDa, while 32kDa and 45kDa proteins were the most immunoreactive with sera from naturally infected bovines.Universidade Federal de Santa Maria2009-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000900024Ciência Rural v.39 n.9 2009reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782009000900024info:eu-repo/semantics/openAccessLafetá,Bárbara NobreCastro,Elaine Cristina deSilva,Nivaldo daeng2009-12-11T00:00:00ZRevista
dc.title.none.fl_str_mv Protein and antigen profiles of Leptospira interrogans serovar Hardjo
title Protein and antigen profiles of Leptospira interrogans serovar Hardjo
spellingShingle Protein and antigen profiles of Leptospira interrogans serovar Hardjo
Lafetá,Bárbara Nobre
Leptospira interrogans
outer membrane protein
hardjoprajitno
immunomodulatory proteins
title_short Protein and antigen profiles of Leptospira interrogans serovar Hardjo
title_full Protein and antigen profiles of Leptospira interrogans serovar Hardjo
title_fullStr Protein and antigen profiles of Leptospira interrogans serovar Hardjo
title_full_unstemmed Protein and antigen profiles of Leptospira interrogans serovar Hardjo
title_sort Protein and antigen profiles of Leptospira interrogans serovar Hardjo
author Lafetá,Bárbara Nobre
author_facet Lafetá,Bárbara Nobre
Castro,Elaine Cristina de
Silva,Nivaldo da
author_role author
author2 Castro,Elaine Cristina de
Silva,Nivaldo da
author2_role author
author
dc.contributor.author.fl_str_mv Lafetá,Bárbara Nobre
Castro,Elaine Cristina de
Silva,Nivaldo da
dc.subject.por.fl_str_mv Leptospira interrogans
outer membrane protein
hardjoprajitno
immunomodulatory proteins
topic Leptospira interrogans
outer membrane protein
hardjoprajitno
immunomodulatory proteins
description The protein profile of the outer membrane of Leptospira interrogans serovar Hardjo subtype hardjoprajitno associated with the bovine natural immune response was investigated. The outer membrane proteins were extracted utilizing Triton X114 and precipitated with acetone. The protein sample was then resolved by SDS-PAGE and reacted in western blot against sera from a hyperimmune rabbit and from naturally infected bovines. In silver stained gels, 14 protein bands were observed, among which four proteins, with 22, 29, 47 and 63kDa, appeared as major constituents. Western blot tests with hyperimmune rabbit antiserum detected bands corresponding to proteins with 35; 27; 24; 21; 17 and 14kDa, while 32kDa and 45kDa proteins were the most immunoreactive with sera from naturally infected bovines.
publishDate 2009
dc.date.none.fl_str_mv 2009-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000900024
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000900024
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-84782009000900024
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.39 n.9 2009
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
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