Sugarcane micropropagation using light emitting diodes and adjustment in growth-medium sucrose concentration
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000700005 |
Resumo: | The aim of this research was to evaluate the use of light emitting diodes (LEDs) instead of white fluorescent lamps as light source and adequate growth-medium sucrose concentration for sugarcane micropropagation (Saccharum officinarum L.). Sugarcane (RB 872552 variety) bud explants were evaluated during the multiplication and rooting phases under controlled growth-room conditions. Different light sources (blue, red and green LEDs; Growlux and white fluorescent lamps) and different medium sucrose concentrations (0; 15; 30 and 45g L-1) were used, maintaining constant light intensity (20µmol m-2 s-1), photoperiod (16h) and temperature (25+2°C). The experiment was a completely randomized design, and treatments were arranged in a 5x4 factorial (five light sources and four medium sucrose concentrations) with six replications. Sugarcane bud growth was satisfactory under the three LED types studied. The presence of sucrose in growth media was essential for bud multiplication and rooting. Nevertheless, each light source requires the respective medium sucrose concentration adjustment for best results. Red LEDs provided a significantly high multiplication rate (although not the highest) with 8.5 buds per sub-culture and 34.9g L-1 of sucrose; also, the highest bud length (33.3mm) and the best plantlet acclimatization. Therefore, LED sources can advantageously substitute fluorescent lamps in laboratories of sugarcane micropropagation. |
id |
UFSM-2_1b89662d01976379912d384a20ea61a4 |
---|---|
oai_identifier_str |
oai:scielo:S0103-84782013000700005 |
network_acronym_str |
UFSM-2 |
network_name_str |
Ciência rural (Online) |
repository_id_str |
|
spelling |
Sugarcane micropropagation using light emitting diodes and adjustment in growth-medium sucrose concentrationSaccharum officinarumLEDlight sourcetissue culturewhite fluorescent lampThe aim of this research was to evaluate the use of light emitting diodes (LEDs) instead of white fluorescent lamps as light source and adequate growth-medium sucrose concentration for sugarcane micropropagation (Saccharum officinarum L.). Sugarcane (RB 872552 variety) bud explants were evaluated during the multiplication and rooting phases under controlled growth-room conditions. Different light sources (blue, red and green LEDs; Growlux and white fluorescent lamps) and different medium sucrose concentrations (0; 15; 30 and 45g L-1) were used, maintaining constant light intensity (20µmol m-2 s-1), photoperiod (16h) and temperature (25+2°C). The experiment was a completely randomized design, and treatments were arranged in a 5x4 factorial (five light sources and four medium sucrose concentrations) with six replications. Sugarcane bud growth was satisfactory under the three LED types studied. The presence of sucrose in growth media was essential for bud multiplication and rooting. Nevertheless, each light source requires the respective medium sucrose concentration adjustment for best results. Red LEDs provided a significantly high multiplication rate (although not the highest) with 8.5 buds per sub-culture and 34.9g L-1 of sucrose; also, the highest bud length (33.3mm) and the best plantlet acclimatization. Therefore, LED sources can advantageously substitute fluorescent lamps in laboratories of sugarcane micropropagation.Universidade Federal de Santa Maria2013-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000700005Ciência Rural v.43 n.7 2013reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782013000700005info:eu-repo/semantics/openAccessRocha,Paulo Sérgio Gomes daOliveira,Roberto Pedroso deScivittaro,Walkyria Buenoeng2013-07-30T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Sugarcane micropropagation using light emitting diodes and adjustment in growth-medium sucrose concentration |
title |
Sugarcane micropropagation using light emitting diodes and adjustment in growth-medium sucrose concentration |
spellingShingle |
Sugarcane micropropagation using light emitting diodes and adjustment in growth-medium sucrose concentration Rocha,Paulo Sérgio Gomes da Saccharum officinarum LED light source tissue culture white fluorescent lamp |
title_short |
Sugarcane micropropagation using light emitting diodes and adjustment in growth-medium sucrose concentration |
title_full |
Sugarcane micropropagation using light emitting diodes and adjustment in growth-medium sucrose concentration |
title_fullStr |
Sugarcane micropropagation using light emitting diodes and adjustment in growth-medium sucrose concentration |
title_full_unstemmed |
Sugarcane micropropagation using light emitting diodes and adjustment in growth-medium sucrose concentration |
title_sort |
Sugarcane micropropagation using light emitting diodes and adjustment in growth-medium sucrose concentration |
author |
Rocha,Paulo Sérgio Gomes da |
author_facet |
Rocha,Paulo Sérgio Gomes da Oliveira,Roberto Pedroso de Scivittaro,Walkyria Bueno |
author_role |
author |
author2 |
Oliveira,Roberto Pedroso de Scivittaro,Walkyria Bueno |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Rocha,Paulo Sérgio Gomes da Oliveira,Roberto Pedroso de Scivittaro,Walkyria Bueno |
dc.subject.por.fl_str_mv |
Saccharum officinarum LED light source tissue culture white fluorescent lamp |
topic |
Saccharum officinarum LED light source tissue culture white fluorescent lamp |
description |
The aim of this research was to evaluate the use of light emitting diodes (LEDs) instead of white fluorescent lamps as light source and adequate growth-medium sucrose concentration for sugarcane micropropagation (Saccharum officinarum L.). Sugarcane (RB 872552 variety) bud explants were evaluated during the multiplication and rooting phases under controlled growth-room conditions. Different light sources (blue, red and green LEDs; Growlux and white fluorescent lamps) and different medium sucrose concentrations (0; 15; 30 and 45g L-1) were used, maintaining constant light intensity (20µmol m-2 s-1), photoperiod (16h) and temperature (25+2°C). The experiment was a completely randomized design, and treatments were arranged in a 5x4 factorial (five light sources and four medium sucrose concentrations) with six replications. Sugarcane bud growth was satisfactory under the three LED types studied. The presence of sucrose in growth media was essential for bud multiplication and rooting. Nevertheless, each light source requires the respective medium sucrose concentration adjustment for best results. Red LEDs provided a significantly high multiplication rate (although not the highest) with 8.5 buds per sub-culture and 34.9g L-1 of sucrose; also, the highest bud length (33.3mm) and the best plantlet acclimatization. Therefore, LED sources can advantageously substitute fluorescent lamps in laboratories of sugarcane micropropagation. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-07-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000700005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000700005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-84782013000700005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.43 n.7 2013 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140543333466112 |