Antibiotic resistance and virulence factors among Escherichia coli isolates from avian organic fertilizer
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200451 |
Resumo: | ABSTRACT: Brazilian poultry industry generates large amounts of organic waste, such as chicken litter, which is often used in agriculture. Among the bacteria present in organic fertilizer are members of the Enterobacteriaceae family, such as Escherichia coli. The aim of this study was to analyze the profile of virulence factors and antimicrobial resistance of E. coli isolates from avian organic fertilizer. A total of 47 E. coli strains were tested through Polymerase chain reaction to detect virulence genes (hlyF, iss, ompT, iutA and iroN). Fourteen antimicrobials were used to test antimicrobial susceptibility in the strains. Genes characteristic of Avian Pathogenic E. coli (APEC) were reported among the strains, with the hlyF, iss and ompT genes being the most prevalent. The isolates showed high resistance (˃50%) to tetracycline, gentamicin, cefotaxime, nitrofurantoin, trimethoprim-sulfamethoxazole and ampicillin. Multidrug resistance was reported in a great number of strains (>70%). The results showed the presence of APEC cells with virulence genes and antimicrobial resistance after 15 days of the windrowing process in poultry houses, it means this process should be improved to eliminate these cells. |
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Antibiotic resistance and virulence factors among Escherichia coli isolates from avian organic fertilizerpoultry productionEnterobacteriaceazoonotic riskvirulence factorsABSTRACT: Brazilian poultry industry generates large amounts of organic waste, such as chicken litter, which is often used in agriculture. Among the bacteria present in organic fertilizer are members of the Enterobacteriaceae family, such as Escherichia coli. The aim of this study was to analyze the profile of virulence factors and antimicrobial resistance of E. coli isolates from avian organic fertilizer. A total of 47 E. coli strains were tested through Polymerase chain reaction to detect virulence genes (hlyF, iss, ompT, iutA and iroN). Fourteen antimicrobials were used to test antimicrobial susceptibility in the strains. Genes characteristic of Avian Pathogenic E. coli (APEC) were reported among the strains, with the hlyF, iss and ompT genes being the most prevalent. The isolates showed high resistance (˃50%) to tetracycline, gentamicin, cefotaxime, nitrofurantoin, trimethoprim-sulfamethoxazole and ampicillin. Multidrug resistance was reported in a great number of strains (>70%). The results showed the presence of APEC cells with virulence genes and antimicrobial resistance after 15 days of the windrowing process in poultry houses, it means this process should be improved to eliminate these cells.Universidade Federal de Santa Maria2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200451Ciência Rural v.50 n.2 2020reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20180849info:eu-repo/semantics/openAccessAgostinho,Juliana Maria AvanciCardozo,Marita VedovelliBorzi,Mariana MoneziMarin,José Moacireng2020-03-09T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Antibiotic resistance and virulence factors among Escherichia coli isolates from avian organic fertilizer |
title |
Antibiotic resistance and virulence factors among Escherichia coli isolates from avian organic fertilizer |
spellingShingle |
Antibiotic resistance and virulence factors among Escherichia coli isolates from avian organic fertilizer Agostinho,Juliana Maria Avanci poultry production Enterobacteriacea zoonotic risk virulence factors |
title_short |
Antibiotic resistance and virulence factors among Escherichia coli isolates from avian organic fertilizer |
title_full |
Antibiotic resistance and virulence factors among Escherichia coli isolates from avian organic fertilizer |
title_fullStr |
Antibiotic resistance and virulence factors among Escherichia coli isolates from avian organic fertilizer |
title_full_unstemmed |
Antibiotic resistance and virulence factors among Escherichia coli isolates from avian organic fertilizer |
title_sort |
Antibiotic resistance and virulence factors among Escherichia coli isolates from avian organic fertilizer |
author |
Agostinho,Juliana Maria Avanci |
author_facet |
Agostinho,Juliana Maria Avanci Cardozo,Marita Vedovelli Borzi,Mariana Monezi Marin,José Moacir |
author_role |
author |
author2 |
Cardozo,Marita Vedovelli Borzi,Mariana Monezi Marin,José Moacir |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Agostinho,Juliana Maria Avanci Cardozo,Marita Vedovelli Borzi,Mariana Monezi Marin,José Moacir |
dc.subject.por.fl_str_mv |
poultry production Enterobacteriacea zoonotic risk virulence factors |
topic |
poultry production Enterobacteriacea zoonotic risk virulence factors |
description |
ABSTRACT: Brazilian poultry industry generates large amounts of organic waste, such as chicken litter, which is often used in agriculture. Among the bacteria present in organic fertilizer are members of the Enterobacteriaceae family, such as Escherichia coli. The aim of this study was to analyze the profile of virulence factors and antimicrobial resistance of E. coli isolates from avian organic fertilizer. A total of 47 E. coli strains were tested through Polymerase chain reaction to detect virulence genes (hlyF, iss, ompT, iutA and iroN). Fourteen antimicrobials were used to test antimicrobial susceptibility in the strains. Genes characteristic of Avian Pathogenic E. coli (APEC) were reported among the strains, with the hlyF, iss and ompT genes being the most prevalent. The isolates showed high resistance (˃50%) to tetracycline, gentamicin, cefotaxime, nitrofurantoin, trimethoprim-sulfamethoxazole and ampicillin. Multidrug resistance was reported in a great number of strains (>70%). The results showed the presence of APEC cells with virulence genes and antimicrobial resistance after 15 days of the windrowing process in poultry houses, it means this process should be improved to eliminate these cells. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200451 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200451 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20180849 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.50 n.2 2020 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140554387554304 |