An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horses

Detalhes bibliográficos
Autor(a) principal: Baldani,Cristiane Divan
Data de Publicação: 2004
Outros Autores: Machado,Rosangela Zacarias, Botteon,Paulo de Tarso Landgraf, Takakura,Felipe Santoro, Massard,Carlos Luiz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000500031
Resumo: A crude antigenic preparation of Babesia equi was used to develop and establish the suitability of an enzyme-linked immunosorbent assay (ELISA) for the detection of parasite carriers. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkboard titrations. The specificity and sensitivity of the ELISA were 100 %. A total of 90 serum samples were taken from horses from the Northeast region of São Paulo State and examined for diagnosis of equine B. equi infection by ELISA. Approximately 75% (n=67) of all the horses tested were found serologically positive for B. equi. These results suggest that the ELISA described may prove to be an appropriate serological test for epidemiological studies on B. equi infections in the field and that equine piroplasmosis is a cause for serious concern in the State of São Paulo, Brazil.
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spelling An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horsesBabesia equiequine piroplasmosisELISAA crude antigenic preparation of Babesia equi was used to develop and establish the suitability of an enzyme-linked immunosorbent assay (ELISA) for the detection of parasite carriers. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkboard titrations. The specificity and sensitivity of the ELISA were 100 %. A total of 90 serum samples were taken from horses from the Northeast region of São Paulo State and examined for diagnosis of equine B. equi infection by ELISA. Approximately 75% (n=67) of all the horses tested were found serologically positive for B. equi. These results suggest that the ELISA described may prove to be an appropriate serological test for epidemiological studies on B. equi infections in the field and that equine piroplasmosis is a cause for serious concern in the State of São Paulo, Brazil.Universidade Federal de Santa Maria2004-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000500031Ciência Rural v.34 n.5 2004reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782004000500031info:eu-repo/semantics/openAccessBaldani,Cristiane DivanMachado,Rosangela ZacariasBotteon,Paulo de Tarso LandgrafTakakura,Felipe SantoroMassard,Carlos Luizeng2004-09-03T00:00:00ZRevista
dc.title.none.fl_str_mv An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horses
title An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horses
spellingShingle An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horses
Baldani,Cristiane Divan
Babesia equi
equine piroplasmosis
ELISA
title_short An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horses
title_full An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horses
title_fullStr An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horses
title_full_unstemmed An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horses
title_sort An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horses
author Baldani,Cristiane Divan
author_facet Baldani,Cristiane Divan
Machado,Rosangela Zacarias
Botteon,Paulo de Tarso Landgraf
Takakura,Felipe Santoro
Massard,Carlos Luiz
author_role author
author2 Machado,Rosangela Zacarias
Botteon,Paulo de Tarso Landgraf
Takakura,Felipe Santoro
Massard,Carlos Luiz
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Baldani,Cristiane Divan
Machado,Rosangela Zacarias
Botteon,Paulo de Tarso Landgraf
Takakura,Felipe Santoro
Massard,Carlos Luiz
dc.subject.por.fl_str_mv Babesia equi
equine piroplasmosis
ELISA
topic Babesia equi
equine piroplasmosis
ELISA
description A crude antigenic preparation of Babesia equi was used to develop and establish the suitability of an enzyme-linked immunosorbent assay (ELISA) for the detection of parasite carriers. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkboard titrations. The specificity and sensitivity of the ELISA were 100 %. A total of 90 serum samples were taken from horses from the Northeast region of São Paulo State and examined for diagnosis of equine B. equi infection by ELISA. Approximately 75% (n=67) of all the horses tested were found serologically positive for B. equi. These results suggest that the ELISA described may prove to be an appropriate serological test for epidemiological studies on B. equi infections in the field and that equine piroplasmosis is a cause for serious concern in the State of São Paulo, Brazil.
publishDate 2004
dc.date.none.fl_str_mv 2004-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000500031
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000500031
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-84782004000500031
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.34 n.5 2004
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1749140525632454656

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