Free-living diazotrophs drive castor bean nitrogen input in tropical semiarid soils

Detalhes bibliográficos
Autor(a) principal: Fracetto,Felipe José Cury
Data de Publicação: 2019
Outros Autores: Fracetto,Giselle Gomes Monteiro, Barros,Felipe Martins do Rêgo, Lira Junior,Mario de Andrade, Siqueira Neto,Marcos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001200252
Resumo: ABSTRACT: There are few studies of microbial diversity in castor bean soils in tropical semiarid environmental. Castor bean products have been widely used around the world justifying the commercial importance of ricinoculture in Brazil’ northeastern semiarid. There is no fertilization or maintenance of litter on the soils from the present study, so we hypothesized that the free-living diazotrophs microorganisms drive the nitrogen (N) input into these soils. We evaluated the communities’ structure and diversity of diazotrophs in tropical semiarid soils with i-Castor bean 50 years of cultivation intercropping with maize (CB-50); ii-Only Castor bean 10 years of cultivation (CB-10) and iii-soil under Caatinga vegetation (CAA). Nitrogenase enzyme activity was performed by the acetylene reduction assay (ARA) and the community profile of nifH gene was separated by denaturing gradient gel polyacrylamide electrophoresis (DGGE). Diazotrophs diversity was estimated by the Shannon (H’) and Simpson (D) indexes. Based on the maintenance of soil N stocks and N-microbial biomass over time, the activity of free-living diazotrophs was determinant in the N-input into these soils. The nifH gene was present and its profiles grouped the CB-50 with CAA treatments, so it reinforced the N-fixing by diazotrophs, which presented a greater diversity in the cultivated soils, even after the land use change for the castor bean implementation.
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spelling Free-living diazotrophs drive castor bean nitrogen input in tropical semiarid soilsnifHmicroorganism diversityricinocultureCaatinga dry forestABSTRACT: There are few studies of microbial diversity in castor bean soils in tropical semiarid environmental. Castor bean products have been widely used around the world justifying the commercial importance of ricinoculture in Brazil’ northeastern semiarid. There is no fertilization or maintenance of litter on the soils from the present study, so we hypothesized that the free-living diazotrophs microorganisms drive the nitrogen (N) input into these soils. We evaluated the communities’ structure and diversity of diazotrophs in tropical semiarid soils with i-Castor bean 50 years of cultivation intercropping with maize (CB-50); ii-Only Castor bean 10 years of cultivation (CB-10) and iii-soil under Caatinga vegetation (CAA). Nitrogenase enzyme activity was performed by the acetylene reduction assay (ARA) and the community profile of nifH gene was separated by denaturing gradient gel polyacrylamide electrophoresis (DGGE). Diazotrophs diversity was estimated by the Shannon (H’) and Simpson (D) indexes. Based on the maintenance of soil N stocks and N-microbial biomass over time, the activity of free-living diazotrophs was determinant in the N-input into these soils. The nifH gene was present and its profiles grouped the CB-50 with CAA treatments, so it reinforced the N-fixing by diazotrophs, which presented a greater diversity in the cultivated soils, even after the land use change for the castor bean implementation.Universidade Federal de Santa Maria2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001200252Ciência Rural v.49 n.12 2019reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20190446info:eu-repo/semantics/openAccessFracetto,Felipe José CuryFracetto,Giselle Gomes MonteiroBarros,Felipe Martins do RêgoLira Junior,Mario de AndradeSiqueira Neto,Marcoseng2019-11-28T00:00:00ZRevista
dc.title.none.fl_str_mv Free-living diazotrophs drive castor bean nitrogen input in tropical semiarid soils
title Free-living diazotrophs drive castor bean nitrogen input in tropical semiarid soils
spellingShingle Free-living diazotrophs drive castor bean nitrogen input in tropical semiarid soils
Fracetto,Felipe José Cury
nifH
microorganism diversity
ricinoculture
Caatinga dry forest
title_short Free-living diazotrophs drive castor bean nitrogen input in tropical semiarid soils
title_full Free-living diazotrophs drive castor bean nitrogen input in tropical semiarid soils
title_fullStr Free-living diazotrophs drive castor bean nitrogen input in tropical semiarid soils
title_full_unstemmed Free-living diazotrophs drive castor bean nitrogen input in tropical semiarid soils
title_sort Free-living diazotrophs drive castor bean nitrogen input in tropical semiarid soils
author Fracetto,Felipe José Cury
author_facet Fracetto,Felipe José Cury
Fracetto,Giselle Gomes Monteiro
Barros,Felipe Martins do Rêgo
Lira Junior,Mario de Andrade
Siqueira Neto,Marcos
author_role author
author2 Fracetto,Giselle Gomes Monteiro
Barros,Felipe Martins do Rêgo
Lira Junior,Mario de Andrade
Siqueira Neto,Marcos
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Fracetto,Felipe José Cury
Fracetto,Giselle Gomes Monteiro
Barros,Felipe Martins do Rêgo
Lira Junior,Mario de Andrade
Siqueira Neto,Marcos
dc.subject.por.fl_str_mv nifH
microorganism diversity
ricinoculture
Caatinga dry forest
topic nifH
microorganism diversity
ricinoculture
Caatinga dry forest
description ABSTRACT: There are few studies of microbial diversity in castor bean soils in tropical semiarid environmental. Castor bean products have been widely used around the world justifying the commercial importance of ricinoculture in Brazil’ northeastern semiarid. There is no fertilization or maintenance of litter on the soils from the present study, so we hypothesized that the free-living diazotrophs microorganisms drive the nitrogen (N) input into these soils. We evaluated the communities’ structure and diversity of diazotrophs in tropical semiarid soils with i-Castor bean 50 years of cultivation intercropping with maize (CB-50); ii-Only Castor bean 10 years of cultivation (CB-10) and iii-soil under Caatinga vegetation (CAA). Nitrogenase enzyme activity was performed by the acetylene reduction assay (ARA) and the community profile of nifH gene was separated by denaturing gradient gel polyacrylamide electrophoresis (DGGE). Diazotrophs diversity was estimated by the Shannon (H’) and Simpson (D) indexes. Based on the maintenance of soil N stocks and N-microbial biomass over time, the activity of free-living diazotrophs was determinant in the N-input into these soils. The nifH gene was present and its profiles grouped the CB-50 with CAA treatments, so it reinforced the N-fixing by diazotrophs, which presented a greater diversity in the cultivated soils, even after the land use change for the castor bean implementation.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001200252
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001200252
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20190446
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.49 n.12 2019
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
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repository.mail.fl_str_mv
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