Comparison of nine DNA extraction methods for the diagnosis of bovine tuberculosis by real time PCR

Detalhes bibliográficos
Autor(a) principal: Moura,André
Data de Publicação: 2016
Outros Autores: Hodon,Mikael Arrais, Soares Filho,Paulo Martins, Issa,Marina de Azevedo, Oliveira,Ana Paula Ferreira de, Fonseca Júnior,Antônio Augusto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000701223
Resumo: ABSTRACT: Bovine tuberculosis is an infectious disease with a high impact on the cattle industry, particularly in developing countries. PCR is a very sensitive method for detection of infectious agents, but the sensitivity of molecular diagnosis is largely dependent on the efficiency of the DNA extraction methods. The objective of this study was to evaluate DNA extraction methods for direct detection of Mycobacterium bovis in bovine tissue. Nine commercial kits for DNA extraction were evaluated when combined with two real time PCRs. The DNeasy Blood & Tissue Kit from QIAGEN showed better performance and sensitivity followed by the DNA Mini Kit RBC and FTA Elute Micro Card. Results suggested that, even when the analytical sensitivity of the qPCR is very high, the extraction method can influence the diagnostic sensitivity.
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spelling Comparison of nine DNA extraction methods for the diagnosis of bovine tuberculosis by real time PCRbovine tuberculosisDNA extractionreal time PCRABSTRACT: Bovine tuberculosis is an infectious disease with a high impact on the cattle industry, particularly in developing countries. PCR is a very sensitive method for detection of infectious agents, but the sensitivity of molecular diagnosis is largely dependent on the efficiency of the DNA extraction methods. The objective of this study was to evaluate DNA extraction methods for direct detection of Mycobacterium bovis in bovine tissue. Nine commercial kits for DNA extraction were evaluated when combined with two real time PCRs. The DNeasy Blood & Tissue Kit from QIAGEN showed better performance and sensitivity followed by the DNA Mini Kit RBC and FTA Elute Micro Card. Results suggested that, even when the analytical sensitivity of the qPCR is very high, the extraction method can influence the diagnostic sensitivity.Universidade Federal de Santa Maria2016-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000701223Ciência Rural v.46 n.7 2016reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20151489info:eu-repo/semantics/openAccessMoura,AndréHodon,Mikael ArraisSoares Filho,Paulo MartinsIssa,Marina de AzevedoOliveira,Ana Paula Ferreira deFonseca Júnior,Antônio Augustoeng2016-06-16T00:00:00ZRevista
dc.title.none.fl_str_mv Comparison of nine DNA extraction methods for the diagnosis of bovine tuberculosis by real time PCR
title Comparison of nine DNA extraction methods for the diagnosis of bovine tuberculosis by real time PCR
spellingShingle Comparison of nine DNA extraction methods for the diagnosis of bovine tuberculosis by real time PCR
Moura,André
bovine tuberculosis
DNA extraction
real time PCR
title_short Comparison of nine DNA extraction methods for the diagnosis of bovine tuberculosis by real time PCR
title_full Comparison of nine DNA extraction methods for the diagnosis of bovine tuberculosis by real time PCR
title_fullStr Comparison of nine DNA extraction methods for the diagnosis of bovine tuberculosis by real time PCR
title_full_unstemmed Comparison of nine DNA extraction methods for the diagnosis of bovine tuberculosis by real time PCR
title_sort Comparison of nine DNA extraction methods for the diagnosis of bovine tuberculosis by real time PCR
author Moura,André
author_facet Moura,André
Hodon,Mikael Arrais
Soares Filho,Paulo Martins
Issa,Marina de Azevedo
Oliveira,Ana Paula Ferreira de
Fonseca Júnior,Antônio Augusto
author_role author
author2 Hodon,Mikael Arrais
Soares Filho,Paulo Martins
Issa,Marina de Azevedo
Oliveira,Ana Paula Ferreira de
Fonseca Júnior,Antônio Augusto
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Moura,André
Hodon,Mikael Arrais
Soares Filho,Paulo Martins
Issa,Marina de Azevedo
Oliveira,Ana Paula Ferreira de
Fonseca Júnior,Antônio Augusto
dc.subject.por.fl_str_mv bovine tuberculosis
DNA extraction
real time PCR
topic bovine tuberculosis
DNA extraction
real time PCR
description ABSTRACT: Bovine tuberculosis is an infectious disease with a high impact on the cattle industry, particularly in developing countries. PCR is a very sensitive method for detection of infectious agents, but the sensitivity of molecular diagnosis is largely dependent on the efficiency of the DNA extraction methods. The objective of this study was to evaluate DNA extraction methods for direct detection of Mycobacterium bovis in bovine tissue. Nine commercial kits for DNA extraction were evaluated when combined with two real time PCRs. The DNeasy Blood & Tissue Kit from QIAGEN showed better performance and sensitivity followed by the DNA Mini Kit RBC and FTA Elute Micro Card. Results suggested that, even when the analytical sensitivity of the qPCR is very high, the extraction method can influence the diagnostic sensitivity.
publishDate 2016
dc.date.none.fl_str_mv 2016-07-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000701223
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000701223
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20151489
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.46 n.7 2016
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
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