Activity of the antimicrobial peptide P34 against bovine alphaherpesvirus type 1
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000600451 |
Resumo: | ABSTRACT: Previous studies have demonstrated the antimicrobial activity of the peptide P34. In this study, the antiviral potential of P34 and the in vitro mechanism of action were investigated against bovine alphaherpesvirus type 1 (BoHV1). P34 exhibited low toxicity, a high selectivity index (22.9) and a percentage of inhibition of up to 100% in MDBK cells. Results from antiviral assays indicated that P34 did not interact with cell receptors, but it was able to inhibit the viral penetration immediately after pre-adsorption. In addition, BoHV1 growth curve in MDBK cells in the presence of P34 revealed a significant reduction in virus titer only 8h post-infection, also suggesting an important role at late stages of the replicative cycle. Virucidal effect was observed only in cytotoxic concentrations of the peptide. These findings showed that the antimicrobial peptide P34 may be considered as a potential novel inhibitor of in vitro herpesviruses and must encourage further investigation of its antiherpetic activity in animal models as well as against a wide spectrum of viruses. |
id |
UFSM-2_db0356fb8e3bb82769215cad7ae5c4e6 |
---|---|
oai_identifier_str |
oai:scielo:S0103-84782017000600451 |
network_acronym_str |
UFSM-2 |
network_name_str |
Ciência rural (Online) |
repository_id_str |
|
spelling |
Activity of the antimicrobial peptide P34 against bovine alphaherpesvirus type 1antiviralherpesviruspeptideABSTRACT: Previous studies have demonstrated the antimicrobial activity of the peptide P34. In this study, the antiviral potential of P34 and the in vitro mechanism of action were investigated against bovine alphaherpesvirus type 1 (BoHV1). P34 exhibited low toxicity, a high selectivity index (22.9) and a percentage of inhibition of up to 100% in MDBK cells. Results from antiviral assays indicated that P34 did not interact with cell receptors, but it was able to inhibit the viral penetration immediately after pre-adsorption. In addition, BoHV1 growth curve in MDBK cells in the presence of P34 revealed a significant reduction in virus titer only 8h post-infection, also suggesting an important role at late stages of the replicative cycle. Virucidal effect was observed only in cytotoxic concentrations of the peptide. These findings showed that the antimicrobial peptide P34 may be considered as a potential novel inhibitor of in vitro herpesviruses and must encourage further investigation of its antiherpetic activity in animal models as well as against a wide spectrum of viruses.Universidade Federal de Santa Maria2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000600451Ciência Rural v.47 n.6 2017reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20160668info:eu-repo/semantics/openAccessCastro,Clarissa Caetano deSilva,Débora Scopel eCosta,Géssica AracéliFischer,GefersonVargas,Gilberto D’AvilaBrandelli,AdrianoLima,Marcelo deMotta,Amanda de Souza daHübner,Silvia de Oliveiraeng2017-04-11T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Activity of the antimicrobial peptide P34 against bovine alphaherpesvirus type 1 |
title |
Activity of the antimicrobial peptide P34 against bovine alphaherpesvirus type 1 |
spellingShingle |
Activity of the antimicrobial peptide P34 against bovine alphaherpesvirus type 1 Castro,Clarissa Caetano de antiviral herpesvirus peptide |
title_short |
Activity of the antimicrobial peptide P34 against bovine alphaherpesvirus type 1 |
title_full |
Activity of the antimicrobial peptide P34 against bovine alphaherpesvirus type 1 |
title_fullStr |
Activity of the antimicrobial peptide P34 against bovine alphaherpesvirus type 1 |
title_full_unstemmed |
Activity of the antimicrobial peptide P34 against bovine alphaherpesvirus type 1 |
title_sort |
Activity of the antimicrobial peptide P34 against bovine alphaherpesvirus type 1 |
author |
Castro,Clarissa Caetano de |
author_facet |
Castro,Clarissa Caetano de Silva,Débora Scopel e Costa,Géssica Aracéli Fischer,Geferson Vargas,Gilberto D’Avila Brandelli,Adriano Lima,Marcelo de Motta,Amanda de Souza da Hübner,Silvia de Oliveira |
author_role |
author |
author2 |
Silva,Débora Scopel e Costa,Géssica Aracéli Fischer,Geferson Vargas,Gilberto D’Avila Brandelli,Adriano Lima,Marcelo de Motta,Amanda de Souza da Hübner,Silvia de Oliveira |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Castro,Clarissa Caetano de Silva,Débora Scopel e Costa,Géssica Aracéli Fischer,Geferson Vargas,Gilberto D’Avila Brandelli,Adriano Lima,Marcelo de Motta,Amanda de Souza da Hübner,Silvia de Oliveira |
dc.subject.por.fl_str_mv |
antiviral herpesvirus peptide |
topic |
antiviral herpesvirus peptide |
description |
ABSTRACT: Previous studies have demonstrated the antimicrobial activity of the peptide P34. In this study, the antiviral potential of P34 and the in vitro mechanism of action were investigated against bovine alphaherpesvirus type 1 (BoHV1). P34 exhibited low toxicity, a high selectivity index (22.9) and a percentage of inhibition of up to 100% in MDBK cells. Results from antiviral assays indicated that P34 did not interact with cell receptors, but it was able to inhibit the viral penetration immediately after pre-adsorption. In addition, BoHV1 growth curve in MDBK cells in the presence of P34 revealed a significant reduction in virus titer only 8h post-infection, also suggesting an important role at late stages of the replicative cycle. Virucidal effect was observed only in cytotoxic concentrations of the peptide. These findings showed that the antimicrobial peptide P34 may be considered as a potential novel inhibitor of in vitro herpesviruses and must encourage further investigation of its antiherpetic activity in animal models as well as against a wide spectrum of viruses. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000600451 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000600451 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20160668 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.47 n.6 2017 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140551341441024 |