Bovine abortion by a vaccine strain of Bacillus anthracis

Detalhes bibliográficos
Autor(a) principal: Bassuino,Daniele Mariath
Data de Publicação: 2020
Outros Autores: Siqueira,Franciele Maboni, Konradt,Guilherme, Vielmo,Andréia, Rolim,Verônica Machado, Gonçalves,Maiara Aline, Cibulski,Samuel Paulo, Snel,Gustavo, Mayer,Fabiana Quoos, Vargas,Agueda Castagna de, Driemeier,David, Pavarini,Saulo Petinatti
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001200554
Resumo: ABSTRACT: This paper reports the abortion of a male Aberdeen Angus bovine by a vaccine strain of Bacillus anthracis, describing the pathological and microbiological findings and the genome sequence. Necropsy findings included multifocal areas of hemorrhage in different organs. Histologically, various organs showed hemorrhage, fibrin exudation, necrosis associated with countless bacillary bacterial clumps and severe neutrophilic inflammatory infiltrate. In the microbiological examination, numerous rough, nonhemolytic, gray and dry colonies with irregular edges were isolated from liver, lung and abomasum content samples. Gram staining revealed square-ended Gram-positive rods arranged in chains. B. anthracis identification was confirmed by detection of the molecular chromosomal marker Ba813. The genomes from the isolated B. anthracis (named SPV842_15) and from the isolated vaccinal strain (Brazilian vaccinal strain), which was recovered from a commercial vaccine used in the pregnant cow, were sequenced. Genomic comparisons displayed a high level of nucleotide identity in the comparisons between B. anthracis SPV842_15 and the B. anthracis Brazilian vaccinal strain (98,2%). Furthermore, in both strains, only the plasmid pX01 sequence was detected. Although, vaccination against anthrax is characterized by an elevated protective profile and very low residual virulence, immunization with Sterne strains can cause abortion in cattle, presumably by the plasmid pX01 toxins in rare or special situations.
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spelling Bovine abortion by a vaccine strain of Bacillus anthracisanthraxcattlefetal lossinfectious diseasesABSTRACT: This paper reports the abortion of a male Aberdeen Angus bovine by a vaccine strain of Bacillus anthracis, describing the pathological and microbiological findings and the genome sequence. Necropsy findings included multifocal areas of hemorrhage in different organs. Histologically, various organs showed hemorrhage, fibrin exudation, necrosis associated with countless bacillary bacterial clumps and severe neutrophilic inflammatory infiltrate. In the microbiological examination, numerous rough, nonhemolytic, gray and dry colonies with irregular edges were isolated from liver, lung and abomasum content samples. Gram staining revealed square-ended Gram-positive rods arranged in chains. B. anthracis identification was confirmed by detection of the molecular chromosomal marker Ba813. The genomes from the isolated B. anthracis (named SPV842_15) and from the isolated vaccinal strain (Brazilian vaccinal strain), which was recovered from a commercial vaccine used in the pregnant cow, were sequenced. Genomic comparisons displayed a high level of nucleotide identity in the comparisons between B. anthracis SPV842_15 and the B. anthracis Brazilian vaccinal strain (98,2%). Furthermore, in both strains, only the plasmid pX01 sequence was detected. Although, vaccination against anthrax is characterized by an elevated protective profile and very low residual virulence, immunization with Sterne strains can cause abortion in cattle, presumably by the plasmid pX01 toxins in rare or special situations.Universidade Federal de Santa Maria2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001200554Ciência Rural v.50 n.12 2020reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20200264info:eu-repo/semantics/openAccessBassuino,Daniele MariathSiqueira,Franciele MaboniKonradt,GuilhermeVielmo,AndréiaRolim,Verônica MachadoGonçalves,Maiara AlineCibulski,Samuel PauloSnel,GustavoMayer,Fabiana QuoosVargas,Agueda Castagna deDriemeier,DavidPavarini,Saulo Petinattieng2020-10-20T00:00:00ZRevista
dc.title.none.fl_str_mv Bovine abortion by a vaccine strain of Bacillus anthracis
title Bovine abortion by a vaccine strain of Bacillus anthracis
spellingShingle Bovine abortion by a vaccine strain of Bacillus anthracis
Bassuino,Daniele Mariath
anthrax
cattle
fetal loss
infectious diseases
title_short Bovine abortion by a vaccine strain of Bacillus anthracis
title_full Bovine abortion by a vaccine strain of Bacillus anthracis
title_fullStr Bovine abortion by a vaccine strain of Bacillus anthracis
title_full_unstemmed Bovine abortion by a vaccine strain of Bacillus anthracis
title_sort Bovine abortion by a vaccine strain of Bacillus anthracis
author Bassuino,Daniele Mariath
author_facet Bassuino,Daniele Mariath
Siqueira,Franciele Maboni
Konradt,Guilherme
Vielmo,Andréia
Rolim,Verônica Machado
Gonçalves,Maiara Aline
Cibulski,Samuel Paulo
Snel,Gustavo
Mayer,Fabiana Quoos
Vargas,Agueda Castagna de
Driemeier,David
Pavarini,Saulo Petinatti
author_role author
author2 Siqueira,Franciele Maboni
Konradt,Guilherme
Vielmo,Andréia
Rolim,Verônica Machado
Gonçalves,Maiara Aline
Cibulski,Samuel Paulo
Snel,Gustavo
Mayer,Fabiana Quoos
Vargas,Agueda Castagna de
Driemeier,David
Pavarini,Saulo Petinatti
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bassuino,Daniele Mariath
Siqueira,Franciele Maboni
Konradt,Guilherme
Vielmo,Andréia
Rolim,Verônica Machado
Gonçalves,Maiara Aline
Cibulski,Samuel Paulo
Snel,Gustavo
Mayer,Fabiana Quoos
Vargas,Agueda Castagna de
Driemeier,David
Pavarini,Saulo Petinatti
dc.subject.por.fl_str_mv anthrax
cattle
fetal loss
infectious diseases
topic anthrax
cattle
fetal loss
infectious diseases
description ABSTRACT: This paper reports the abortion of a male Aberdeen Angus bovine by a vaccine strain of Bacillus anthracis, describing the pathological and microbiological findings and the genome sequence. Necropsy findings included multifocal areas of hemorrhage in different organs. Histologically, various organs showed hemorrhage, fibrin exudation, necrosis associated with countless bacillary bacterial clumps and severe neutrophilic inflammatory infiltrate. In the microbiological examination, numerous rough, nonhemolytic, gray and dry colonies with irregular edges were isolated from liver, lung and abomasum content samples. Gram staining revealed square-ended Gram-positive rods arranged in chains. B. anthracis identification was confirmed by detection of the molecular chromosomal marker Ba813. The genomes from the isolated B. anthracis (named SPV842_15) and from the isolated vaccinal strain (Brazilian vaccinal strain), which was recovered from a commercial vaccine used in the pregnant cow, were sequenced. Genomic comparisons displayed a high level of nucleotide identity in the comparisons between B. anthracis SPV842_15 and the B. anthracis Brazilian vaccinal strain (98,2%). Furthermore, in both strains, only the plasmid pX01 sequence was detected. Although, vaccination against anthrax is characterized by an elevated protective profile and very low residual virulence, immunization with Sterne strains can cause abortion in cattle, presumably by the plasmid pX01 toxins in rare or special situations.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001200554
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001200554
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20200264
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.50 n.12 2020
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
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