Guanidinoacetic acid supplementation on growth performance and molecular mechanisms of lean mass gain in nursery pigs

Detalhes bibliográficos
Autor(a) principal: Valini,Graziela Alves da Cunha
Data de Publicação: 2020
Outros Autores: Duarte,Marcio de Souza, Rodrigues,Gustavo de Amorim, Veroneze,Renata, Saraiva,Alysson, Hausman,Gary, Rocha,Gabriel Cipriano
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000800653
Resumo: ABSTRACT: The objective with the present study was to evaluate the effect of guanidinoacetic acid (GAA) on the growth performance of nursery piglets as well as a possible molecular mechanism of action on lean mass gain. Seventy-two pigs, weaned at 21 d, weighing 6.80 ± 1.2 kg were distributed in a completely randomized design into one of three dietary treatments (control, control + 1.2 g/kg GAA or control + 2.4 g/kg GAA) and 8 replicates per treatment. The control diet was an animal protein-free diet based on corn and soybean meal. Body weight, average daily weight gain, average daily feed intake and feed efficiency were evaluated at 35, 49, and 56 days. At the end of the experiment, one animal per pen was slaughtered and samples of the vastus lateralis muscle were collected for RT-qPCR and protein abundance analysis. Overall (from 21 to 56 d), GAA supplementation improved feed efficiency (P < 0.03). Skeletal muscle of pigs fed with GAA diet had greater mRNA expression of Akt (P < 0.04) and RPS6KB2 (P<0.01). In conclusion, supplementation with 2.4 g/kg GAA to nursery piglets improves feed efficiency and activates molecular mechanisms important to lean mass gain.
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spelling Guanidinoacetic acid supplementation on growth performance and molecular mechanisms of lean mass gain in nursery pigsgene expressiongrowth performanceguanidinoacetic acidpigletprotein synthesisABSTRACT: The objective with the present study was to evaluate the effect of guanidinoacetic acid (GAA) on the growth performance of nursery piglets as well as a possible molecular mechanism of action on lean mass gain. Seventy-two pigs, weaned at 21 d, weighing 6.80 ± 1.2 kg were distributed in a completely randomized design into one of three dietary treatments (control, control + 1.2 g/kg GAA or control + 2.4 g/kg GAA) and 8 replicates per treatment. The control diet was an animal protein-free diet based on corn and soybean meal. Body weight, average daily weight gain, average daily feed intake and feed efficiency were evaluated at 35, 49, and 56 days. At the end of the experiment, one animal per pen was slaughtered and samples of the vastus lateralis muscle were collected for RT-qPCR and protein abundance analysis. Overall (from 21 to 56 d), GAA supplementation improved feed efficiency (P < 0.03). Skeletal muscle of pigs fed with GAA diet had greater mRNA expression of Akt (P < 0.04) and RPS6KB2 (P<0.01). In conclusion, supplementation with 2.4 g/kg GAA to nursery piglets improves feed efficiency and activates molecular mechanisms important to lean mass gain.Universidade Federal de Santa Maria2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000800653Ciência Rural v.50 n.8 2020reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20190948info:eu-repo/semantics/openAccessValini,Graziela Alves da CunhaDuarte,Marcio de SouzaRodrigues,Gustavo de AmorimVeroneze,RenataSaraiva,AlyssonHausman,GaryRocha,Gabriel Ciprianoeng2020-07-07T00:00:00ZRevista
dc.title.none.fl_str_mv Guanidinoacetic acid supplementation on growth performance and molecular mechanisms of lean mass gain in nursery pigs
title Guanidinoacetic acid supplementation on growth performance and molecular mechanisms of lean mass gain in nursery pigs
spellingShingle Guanidinoacetic acid supplementation on growth performance and molecular mechanisms of lean mass gain in nursery pigs
Valini,Graziela Alves da Cunha
gene expression
growth performance
guanidinoacetic acid
piglet
protein synthesis
title_short Guanidinoacetic acid supplementation on growth performance and molecular mechanisms of lean mass gain in nursery pigs
title_full Guanidinoacetic acid supplementation on growth performance and molecular mechanisms of lean mass gain in nursery pigs
title_fullStr Guanidinoacetic acid supplementation on growth performance and molecular mechanisms of lean mass gain in nursery pigs
title_full_unstemmed Guanidinoacetic acid supplementation on growth performance and molecular mechanisms of lean mass gain in nursery pigs
title_sort Guanidinoacetic acid supplementation on growth performance and molecular mechanisms of lean mass gain in nursery pigs
author Valini,Graziela Alves da Cunha
author_facet Valini,Graziela Alves da Cunha
Duarte,Marcio de Souza
Rodrigues,Gustavo de Amorim
Veroneze,Renata
Saraiva,Alysson
Hausman,Gary
Rocha,Gabriel Cipriano
author_role author
author2 Duarte,Marcio de Souza
Rodrigues,Gustavo de Amorim
Veroneze,Renata
Saraiva,Alysson
Hausman,Gary
Rocha,Gabriel Cipriano
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Valini,Graziela Alves da Cunha
Duarte,Marcio de Souza
Rodrigues,Gustavo de Amorim
Veroneze,Renata
Saraiva,Alysson
Hausman,Gary
Rocha,Gabriel Cipriano
dc.subject.por.fl_str_mv gene expression
growth performance
guanidinoacetic acid
piglet
protein synthesis
topic gene expression
growth performance
guanidinoacetic acid
piglet
protein synthesis
description ABSTRACT: The objective with the present study was to evaluate the effect of guanidinoacetic acid (GAA) on the growth performance of nursery piglets as well as a possible molecular mechanism of action on lean mass gain. Seventy-two pigs, weaned at 21 d, weighing 6.80 ± 1.2 kg were distributed in a completely randomized design into one of three dietary treatments (control, control + 1.2 g/kg GAA or control + 2.4 g/kg GAA) and 8 replicates per treatment. The control diet was an animal protein-free diet based on corn and soybean meal. Body weight, average daily weight gain, average daily feed intake and feed efficiency were evaluated at 35, 49, and 56 days. At the end of the experiment, one animal per pen was slaughtered and samples of the vastus lateralis muscle were collected for RT-qPCR and protein abundance analysis. Overall (from 21 to 56 d), GAA supplementation improved feed efficiency (P < 0.03). Skeletal muscle of pigs fed with GAA diet had greater mRNA expression of Akt (P < 0.04) and RPS6KB2 (P<0.01). In conclusion, supplementation with 2.4 g/kg GAA to nursery piglets improves feed efficiency and activates molecular mechanisms important to lean mass gain.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000800653
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000800653
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20190948
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.50 n.8 2020
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1749140554853122048

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