Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011001200029 |
Resumo: | The stress imposed on plants by soil flooding constitutes a major barrier to growth and productivity. The identification of soybean varieties that produce higher levels of isoflavones, is necessary as soybeans have been used as human food to reduce risks of chronic diseases. Thus, this study was conducted with the objective of quantifying proteins and isoflavones in soybean cultivars subjected to flooding at various stages of development. The cultivars 'BRS267', 'BRS257' and 'BRS213' were subjected to 15 days of flooding, starting at the stages V6 and V8 and 11 days under stress starting at the stage R4. The proteins in the grain were extracted and quantified and analyzed by SDS-PAGE electrophoresis. Isoflavones were extracted, separated and quantified on HPLC. The electrophoretic analysis of the three cultivars under study revealed the same pattern of banding relative to the total protein regardless of the treatment. However, it was noted that flooding led to an increase in the total contents of isoflavones in the BRS 267 plants flooded in stage R4, remaining constant in other cultivars. |
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Isoflavone and protein content in soybeans grains submitted to flooding at different stages of developmentGlycine maxhypoxiafunctional foodThe stress imposed on plants by soil flooding constitutes a major barrier to growth and productivity. The identification of soybean varieties that produce higher levels of isoflavones, is necessary as soybeans have been used as human food to reduce risks of chronic diseases. Thus, this study was conducted with the objective of quantifying proteins and isoflavones in soybean cultivars subjected to flooding at various stages of development. The cultivars 'BRS267', 'BRS257' and 'BRS213' were subjected to 15 days of flooding, starting at the stages V6 and V8 and 11 days under stress starting at the stage R4. The proteins in the grain were extracted and quantified and analyzed by SDS-PAGE electrophoresis. Isoflavones were extracted, separated and quantified on HPLC. The electrophoretic analysis of the three cultivars under study revealed the same pattern of banding relative to the total protein regardless of the treatment. However, it was noted that flooding led to an increase in the total contents of isoflavones in the BRS 267 plants flooded in stage R4, remaining constant in other cultivars.Universidade Federal de Santa Maria2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011001200029Ciência Rural v.41 n.12 2011reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782011001200029info:eu-repo/semantics/openAccessFante,Camila ArgentaGoulart,Patrícia de Fátima PereiraAlves,José DonizetiHenrique,Paôla de CastroFries,Daniela Deitoseng2012-01-09T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development |
title |
Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development |
spellingShingle |
Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development Fante,Camila Argenta Glycine max hypoxia functional food |
title_short |
Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development |
title_full |
Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development |
title_fullStr |
Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development |
title_full_unstemmed |
Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development |
title_sort |
Isoflavone and protein content in soybeans grains submitted to flooding at different stages of development |
author |
Fante,Camila Argenta |
author_facet |
Fante,Camila Argenta Goulart,Patrícia de Fátima Pereira Alves,José Donizeti Henrique,Paôla de Castro Fries,Daniela Deitos |
author_role |
author |
author2 |
Goulart,Patrícia de Fátima Pereira Alves,José Donizeti Henrique,Paôla de Castro Fries,Daniela Deitos |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Fante,Camila Argenta Goulart,Patrícia de Fátima Pereira Alves,José Donizeti Henrique,Paôla de Castro Fries,Daniela Deitos |
dc.subject.por.fl_str_mv |
Glycine max hypoxia functional food |
topic |
Glycine max hypoxia functional food |
description |
The stress imposed on plants by soil flooding constitutes a major barrier to growth and productivity. The identification of soybean varieties that produce higher levels of isoflavones, is necessary as soybeans have been used as human food to reduce risks of chronic diseases. Thus, this study was conducted with the objective of quantifying proteins and isoflavones in soybean cultivars subjected to flooding at various stages of development. The cultivars 'BRS267', 'BRS257' and 'BRS213' were subjected to 15 days of flooding, starting at the stages V6 and V8 and 11 days under stress starting at the stage R4. The proteins in the grain were extracted and quantified and analyzed by SDS-PAGE electrophoresis. Isoflavones were extracted, separated and quantified on HPLC. The electrophoretic analysis of the three cultivars under study revealed the same pattern of banding relative to the total protein regardless of the treatment. However, it was noted that flooding led to an increase in the total contents of isoflavones in the BRS 267 plants flooded in stage R4, remaining constant in other cultivars. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011001200029 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011001200029 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-84782011001200029 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.41 n.12 2011 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140539685470208 |