Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantum

Detalhes bibliográficos
Autor(a) principal: Oliveira,Vinícius Vasconcelos Gomes de
Data de Publicação: 2016
Outros Autores: Macedo,Simone Regina Barros de, Araújo,Flábio Ribeiro de, Ramos,Carlos Alberto do Nascimento, Ramos,Rafael Antonio Nascimento, Maia,Frederico Celso Lyra, Alves,Leucio Câmara, Silva Junior,Valdemiro Amaro da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400641
Resumo: ABSTRACT: Canine visceral leishmaniasisis an important disease caused by the protozoon Leishmania infantum which affects several organs and systems, including the genital tract. The L. infantum tropism to the male genital system and correlation among parasite load, immunohistochemistry detection and structural changes in these organs is controversy. Therefore, the aim of this study was to evaluate this correlation in the genital organs of the male dogs naturally infected with L. infantum. Samples from testicles, epididymis, prostate, glans penis, prepuce and scrotum were collected from 19 positive adult dogs. Structural changes were observed in the testicles (5.2%), epididymis (2.6%), prepuce (5.2%) and scrotum (5.2%) of the samples positive at immunohistochemistry examination. Conversely, similar structural changes were observed in all tissues negative at immunohistochemistry analysis. Moreover, L. infantum DNA was detected in all organs with the highest parasite load found in testicles, epididymis, and prostate gland. , Testicles had the highest parasite load but the lowest number of inflammatory lesions. These inflammatory lesions were observed in all organs of reproductive system of dogs; however, no correlation was observed between the parasite load and inflammatory lesions of dogs naturally infected with L. infantum.
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spelling Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantumcanine visceral leishmaniasisstructural changesgenital pathologyimmunohistochemistryABSTRACT: Canine visceral leishmaniasisis an important disease caused by the protozoon Leishmania infantum which affects several organs and systems, including the genital tract. The L. infantum tropism to the male genital system and correlation among parasite load, immunohistochemistry detection and structural changes in these organs is controversy. Therefore, the aim of this study was to evaluate this correlation in the genital organs of the male dogs naturally infected with L. infantum. Samples from testicles, epididymis, prostate, glans penis, prepuce and scrotum were collected from 19 positive adult dogs. Structural changes were observed in the testicles (5.2%), epididymis (2.6%), prepuce (5.2%) and scrotum (5.2%) of the samples positive at immunohistochemistry examination. Conversely, similar structural changes were observed in all tissues negative at immunohistochemistry analysis. Moreover, L. infantum DNA was detected in all organs with the highest parasite load found in testicles, epididymis, and prostate gland. , Testicles had the highest parasite load but the lowest number of inflammatory lesions. These inflammatory lesions were observed in all organs of reproductive system of dogs; however, no correlation was observed between the parasite load and inflammatory lesions of dogs naturally infected with L. infantum.Universidade Federal de Santa Maria2016-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400641Ciência Rural v.46 n.4 2016reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20150850info:eu-repo/semantics/openAccessOliveira,Vinícius Vasconcelos Gomes deMacedo,Simone Regina Barros deAraújo,Flábio Ribeiro deRamos,Carlos Alberto do NascimentoRamos,Rafael Antonio NascimentoMaia,Frederico Celso LyraAlves,Leucio CâmaraSilva Junior,Valdemiro Amaro daeng2016-03-30T00:00:00ZRevista
dc.title.none.fl_str_mv Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantum
title Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantum
spellingShingle Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantum
Oliveira,Vinícius Vasconcelos Gomes de
canine visceral leishmaniasis
structural changes
genital pathology
immunohistochemistry
title_short Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantum
title_full Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantum
title_fullStr Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantum
title_full_unstemmed Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantum
title_sort Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantum
author Oliveira,Vinícius Vasconcelos Gomes de
author_facet Oliveira,Vinícius Vasconcelos Gomes de
Macedo,Simone Regina Barros de
Araújo,Flábio Ribeiro de
Ramos,Carlos Alberto do Nascimento
Ramos,Rafael Antonio Nascimento
Maia,Frederico Celso Lyra
Alves,Leucio Câmara
Silva Junior,Valdemiro Amaro da
author_role author
author2 Macedo,Simone Regina Barros de
Araújo,Flábio Ribeiro de
Ramos,Carlos Alberto do Nascimento
Ramos,Rafael Antonio Nascimento
Maia,Frederico Celso Lyra
Alves,Leucio Câmara
Silva Junior,Valdemiro Amaro da
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira,Vinícius Vasconcelos Gomes de
Macedo,Simone Regina Barros de
Araújo,Flábio Ribeiro de
Ramos,Carlos Alberto do Nascimento
Ramos,Rafael Antonio Nascimento
Maia,Frederico Celso Lyra
Alves,Leucio Câmara
Silva Junior,Valdemiro Amaro da
dc.subject.por.fl_str_mv canine visceral leishmaniasis
structural changes
genital pathology
immunohistochemistry
topic canine visceral leishmaniasis
structural changes
genital pathology
immunohistochemistry
description ABSTRACT: Canine visceral leishmaniasisis an important disease caused by the protozoon Leishmania infantum which affects several organs and systems, including the genital tract. The L. infantum tropism to the male genital system and correlation among parasite load, immunohistochemistry detection and structural changes in these organs is controversy. Therefore, the aim of this study was to evaluate this correlation in the genital organs of the male dogs naturally infected with L. infantum. Samples from testicles, epididymis, prostate, glans penis, prepuce and scrotum were collected from 19 positive adult dogs. Structural changes were observed in the testicles (5.2%), epididymis (2.6%), prepuce (5.2%) and scrotum (5.2%) of the samples positive at immunohistochemistry examination. Conversely, similar structural changes were observed in all tissues negative at immunohistochemistry analysis. Moreover, L. infantum DNA was detected in all organs with the highest parasite load found in testicles, epididymis, and prostate gland. , Testicles had the highest parasite load but the lowest number of inflammatory lesions. These inflammatory lesions were observed in all organs of reproductive system of dogs; however, no correlation was observed between the parasite load and inflammatory lesions of dogs naturally infected with L. infantum.
publishDate 2016
dc.date.none.fl_str_mv 2016-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400641
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400641
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20150850
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.46 n.4 2016
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
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