Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantum
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400641 |
Resumo: | ABSTRACT: Canine visceral leishmaniasisis an important disease caused by the protozoon Leishmania infantum which affects several organs and systems, including the genital tract. The L. infantum tropism to the male genital system and correlation among parasite load, immunohistochemistry detection and structural changes in these organs is controversy. Therefore, the aim of this study was to evaluate this correlation in the genital organs of the male dogs naturally infected with L. infantum. Samples from testicles, epididymis, prostate, glans penis, prepuce and scrotum were collected from 19 positive adult dogs. Structural changes were observed in the testicles (5.2%), epididymis (2.6%), prepuce (5.2%) and scrotum (5.2%) of the samples positive at immunohistochemistry examination. Conversely, similar structural changes were observed in all tissues negative at immunohistochemistry analysis. Moreover, L. infantum DNA was detected in all organs with the highest parasite load found in testicles, epididymis, and prostate gland. , Testicles had the highest parasite load but the lowest number of inflammatory lesions. These inflammatory lesions were observed in all organs of reproductive system of dogs; however, no correlation was observed between the parasite load and inflammatory lesions of dogs naturally infected with L. infantum. |
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Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantumcanine visceral leishmaniasisstructural changesgenital pathologyimmunohistochemistryABSTRACT: Canine visceral leishmaniasisis an important disease caused by the protozoon Leishmania infantum which affects several organs and systems, including the genital tract. The L. infantum tropism to the male genital system and correlation among parasite load, immunohistochemistry detection and structural changes in these organs is controversy. Therefore, the aim of this study was to evaluate this correlation in the genital organs of the male dogs naturally infected with L. infantum. Samples from testicles, epididymis, prostate, glans penis, prepuce and scrotum were collected from 19 positive adult dogs. Structural changes were observed in the testicles (5.2%), epididymis (2.6%), prepuce (5.2%) and scrotum (5.2%) of the samples positive at immunohistochemistry examination. Conversely, similar structural changes were observed in all tissues negative at immunohistochemistry analysis. Moreover, L. infantum DNA was detected in all organs with the highest parasite load found in testicles, epididymis, and prostate gland. , Testicles had the highest parasite load but the lowest number of inflammatory lesions. These inflammatory lesions were observed in all organs of reproductive system of dogs; however, no correlation was observed between the parasite load and inflammatory lesions of dogs naturally infected with L. infantum.Universidade Federal de Santa Maria2016-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400641Ciência Rural v.46 n.4 2016reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20150850info:eu-repo/semantics/openAccessOliveira,Vinícius Vasconcelos Gomes deMacedo,Simone Regina Barros deAraújo,Flábio Ribeiro deRamos,Carlos Alberto do NascimentoRamos,Rafael Antonio NascimentoMaia,Frederico Celso LyraAlves,Leucio CâmaraSilva Junior,Valdemiro Amaro daeng2016-03-30T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantum |
title |
Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantum |
spellingShingle |
Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantum Oliveira,Vinícius Vasconcelos Gomes de canine visceral leishmaniasis structural changes genital pathology immunohistochemistry |
title_short |
Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantum |
title_full |
Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantum |
title_fullStr |
Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantum |
title_full_unstemmed |
Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantum |
title_sort |
Histopathological evaluation and parasite quantification (qPCR) in the male dog's genital system after natural infection with Leishmania infantum |
author |
Oliveira,Vinícius Vasconcelos Gomes de |
author_facet |
Oliveira,Vinícius Vasconcelos Gomes de Macedo,Simone Regina Barros de Araújo,Flábio Ribeiro de Ramos,Carlos Alberto do Nascimento Ramos,Rafael Antonio Nascimento Maia,Frederico Celso Lyra Alves,Leucio Câmara Silva Junior,Valdemiro Amaro da |
author_role |
author |
author2 |
Macedo,Simone Regina Barros de Araújo,Flábio Ribeiro de Ramos,Carlos Alberto do Nascimento Ramos,Rafael Antonio Nascimento Maia,Frederico Celso Lyra Alves,Leucio Câmara Silva Junior,Valdemiro Amaro da |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira,Vinícius Vasconcelos Gomes de Macedo,Simone Regina Barros de Araújo,Flábio Ribeiro de Ramos,Carlos Alberto do Nascimento Ramos,Rafael Antonio Nascimento Maia,Frederico Celso Lyra Alves,Leucio Câmara Silva Junior,Valdemiro Amaro da |
dc.subject.por.fl_str_mv |
canine visceral leishmaniasis structural changes genital pathology immunohistochemistry |
topic |
canine visceral leishmaniasis structural changes genital pathology immunohistochemistry |
description |
ABSTRACT: Canine visceral leishmaniasisis an important disease caused by the protozoon Leishmania infantum which affects several organs and systems, including the genital tract. The L. infantum tropism to the male genital system and correlation among parasite load, immunohistochemistry detection and structural changes in these organs is controversy. Therefore, the aim of this study was to evaluate this correlation in the genital organs of the male dogs naturally infected with L. infantum. Samples from testicles, epididymis, prostate, glans penis, prepuce and scrotum were collected from 19 positive adult dogs. Structural changes were observed in the testicles (5.2%), epididymis (2.6%), prepuce (5.2%) and scrotum (5.2%) of the samples positive at immunohistochemistry examination. Conversely, similar structural changes were observed in all tissues negative at immunohistochemistry analysis. Moreover, L. infantum DNA was detected in all organs with the highest parasite load found in testicles, epididymis, and prostate gland. , Testicles had the highest parasite load but the lowest number of inflammatory lesions. These inflammatory lesions were observed in all organs of reproductive system of dogs; however, no correlation was observed between the parasite load and inflammatory lesions of dogs naturally infected with L. infantum. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400641 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400641 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20150850 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.46 n.4 2016 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140548994727936 |