Using qPCR for diagnosis of canine visceral leishmaniasis and quantification of Leishmania sp in different tissues of naturally infected dogs

Detalhes bibliográficos
Autor(a) principal: Solcà, Manuela da Silva
Data de Publicação: 2012
Outros Autores: Bastos, Leila Andrade, Guedes, C. E. S, Gonçalves, Marcelo Bordoni, Nascimento, E. G, dosSantos, Washington Luis Conrado, Oliveira, G. G. S, Fraga, Deborah Bittencourt Mothé, Veras, Patrícia Sampaio Tavares
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/24200
Resumo: FAPESB, INCT-CNPq, PDTIS, PST Veras’ grant (CNPq:306672/2008-1).
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spelling Solcà, Manuela da SilvaBastos, Leila AndradeGuedes, C. E. SGonçalves, Marcelo BordoniNascimento, E. GdosSantos, Washington Luis ConradoOliveira, G. G. SFraga, Deborah Bittencourt MothéVeras, Patrícia Sampaio Tavares2018-01-30T17:11:46Z2018-01-30T17:11:46Z2012SOLCA, M. S. et al. Using qPCR for diagnosis of canine visceral leishmaniasis and quantification of Leishmania sp in different tissues of naturally infected dogs. In: CONGRESSO INTERNACIONAL DE MEDICINA TROPICAL E MALÁRIA, 18 e CONGRESSO DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, 48., 2012, Rio de Janeiro. Resumos... Rio de Janeiro: Sociedade Brasileira de Medicina Tropical, 2012.https://www.arca.fiocruz.br/handle/icict/24200FAPESB, INCT-CNPq, PDTIS, PST Veras’ grant (CNPq:306672/2008-1).Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilCentro de Referência em Doenças Endemicas Pirajá da Silva Posto Piej. Jequié, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Escola de Medicina Veterinária. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilBecause infected dogs are considered to be the main domestic reservoir for Leishmania infantum (syn L. chagasi) in Brazil, the diagnosis of canine visceral leishmaniasis must be made both accurately and promptly. Serologic techniques are very sensitive but present risk of cross-reaction with other diseases. First, the authors standardized a previously described qPCR protocol, targeting the kinetoplast DNA of the parasite. Using this protocol, the authors aimed to determine which tissue confers the most accurate detection of parasite DNA. In a serological surveillance in the municipality of Jequié, an endemic area in Bahia, Brazil, forty-six dogs were randomly selected and classified according to the number of clinical signs of canine visceral leishmaniasisengSociedade Brasileira de Medicina TropicalLeishmaniose Visceral CaninaLeishmaniaAnimaisCãesInfecçãoBaçoCanine Visceral LeishmaniasisLeishmaniaAnimalsDogsInfectionSpleenUsing qPCR for diagnosis of canine visceral leishmaniasis and quantification of Leishmania sp in different tissues of naturally infected dogsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24200/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSolca MS Using...SBMT 2012 Leish085.pdfSolca MS Using...SBMT 2012 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dc.title.pt_BR.fl_str_mv Using qPCR for diagnosis of canine visceral leishmaniasis and quantification of Leishmania sp in different tissues of naturally infected dogs
title Using qPCR for diagnosis of canine visceral leishmaniasis and quantification of Leishmania sp in different tissues of naturally infected dogs
spellingShingle Using qPCR for diagnosis of canine visceral leishmaniasis and quantification of Leishmania sp in different tissues of naturally infected dogs
Solcà, Manuela da Silva
Leishmaniose Visceral Canina
Leishmania
Animais
Cães
Infecção
Baço
Canine Visceral Leishmaniasis
Leishmania
Animals
Dogs
Infection
Spleen
title_short Using qPCR for diagnosis of canine visceral leishmaniasis and quantification of Leishmania sp in different tissues of naturally infected dogs
title_full Using qPCR for diagnosis of canine visceral leishmaniasis and quantification of Leishmania sp in different tissues of naturally infected dogs
title_fullStr Using qPCR for diagnosis of canine visceral leishmaniasis and quantification of Leishmania sp in different tissues of naturally infected dogs
title_full_unstemmed Using qPCR for diagnosis of canine visceral leishmaniasis and quantification of Leishmania sp in different tissues of naturally infected dogs
title_sort Using qPCR for diagnosis of canine visceral leishmaniasis and quantification of Leishmania sp in different tissues of naturally infected dogs
author Solcà, Manuela da Silva
author_facet Solcà, Manuela da Silva
Bastos, Leila Andrade
Guedes, C. E. S
Gonçalves, Marcelo Bordoni
Nascimento, E. G
dosSantos, Washington Luis Conrado
Oliveira, G. G. S
Fraga, Deborah Bittencourt Mothé
Veras, Patrícia Sampaio Tavares
author_role author
author2 Bastos, Leila Andrade
Guedes, C. E. S
Gonçalves, Marcelo Bordoni
Nascimento, E. G
dosSantos, Washington Luis Conrado
Oliveira, G. G. S
Fraga, Deborah Bittencourt Mothé
Veras, Patrícia Sampaio Tavares
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Solcà, Manuela da Silva
Bastos, Leila Andrade
Guedes, C. E. S
Gonçalves, Marcelo Bordoni
Nascimento, E. G
dosSantos, Washington Luis Conrado
Oliveira, G. G. S
Fraga, Deborah Bittencourt Mothé
Veras, Patrícia Sampaio Tavares
dc.subject.other.pt_BR.fl_str_mv Leishmaniose Visceral Canina
Leishmania
Animais
Cães
Infecção
Baço
topic Leishmaniose Visceral Canina
Leishmania
Animais
Cães
Infecção
Baço
Canine Visceral Leishmaniasis
Leishmania
Animals
Dogs
Infection
Spleen
dc.subject.en.pt_BR.fl_str_mv Canine Visceral Leishmaniasis
Leishmania
Animals
Dogs
Infection
Spleen
description FAPESB, INCT-CNPq, PDTIS, PST Veras’ grant (CNPq:306672/2008-1).
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2018-01-30T17:11:46Z
dc.date.available.fl_str_mv 2018-01-30T17:11:46Z
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dc.identifier.citation.fl_str_mv SOLCA, M. S. et al. Using qPCR for diagnosis of canine visceral leishmaniasis and quantification of Leishmania sp in different tissues of naturally infected dogs. In: CONGRESSO INTERNACIONAL DE MEDICINA TROPICAL E MALÁRIA, 18 e CONGRESSO DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, 48., 2012, Rio de Janeiro. Resumos... Rio de Janeiro: Sociedade Brasileira de Medicina Tropical, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/24200
identifier_str_mv SOLCA, M. S. et al. Using qPCR for diagnosis of canine visceral leishmaniasis and quantification of Leishmania sp in different tissues of naturally infected dogs. In: CONGRESSO INTERNACIONAL DE MEDICINA TROPICAL E MALÁRIA, 18 e CONGRESSO DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, 48., 2012, Rio de Janeiro. Resumos... Rio de Janeiro: Sociedade Brasileira de Medicina Tropical, 2012.
url https://www.arca.fiocruz.br/handle/icict/24200
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical
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