Callogenesis in Myracrodruon urundeuva Fr. All.
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência Florestal (Online) |
Texto Completo: | https://periodicos.ufsm.br/cienciaflorestal/article/view/31689 |
Resumo: | Myracrodruon urundeuva Fr. All.is a tree belonging to the family Anacardiaceae, popularly known as ‘aroeira-do-sertão’, which has potential for wood production and medicinal purposes but it is threatened with extinction. This work investigated the callogenesis of the species and biochemically analyzed the calli produced, aiming at further studies of somatic embryogenesis. Leaf explants were inoculated in MS culture medium (Murashige and Skoog, 1962) supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (0.0, 2.5, 10.0 and 20.0 μM), kinetin (0.0, 2.5 and 5.0 μM) and glutamine (0.0, 0.34, 0.68 and 1.37 μM). For the growth curve, the fresh mass of explants with and without calli was determined until the 56th day of culture, at intervals of 7 days. Reducing sugars (RS), sucrose and total soluble sugars (TSS) were measured at each growth curve period. The use of 2,4-D was efficient for callus induction in Myracrodruon urundeuva, and callogenesis was enhanced with the combination of 2,4-D, KIN and glutamine in the nutrient medium. The growth curve of the calli presented sigmoidal shape with five distinct phases: lag, exponential, linear, deceleration and stationary. The content of sucrose and TSS presented different behavior according to the different stages of callus growth, exhibiting accumulation of sugars in the period of preparation for growth, degradation in the exponential phase and once again accumulated these carbohydrates in the final stages (deceleration and stationary). The RS content, except day zero, remained stable during all evaluated periods. The calli of Myracrodruon urundeuva have embryogenic potential, but further studies are suggested to obtain somatic embryos, as well as to identify the period in which they are formed. |
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Callogenesis in Myracrodruon urundeuva Fr. All.Calogênese em Myracrodruon urundeuva Fr. All.Aroeira-do-sertãoCarbohydratesMedicinal plantIn vitro cultureAroeira-do-sertãoCarboidratosPlanta medicinalCultivo in vitroMyracrodruon urundeuva Fr. All.is a tree belonging to the family Anacardiaceae, popularly known as ‘aroeira-do-sertão’, which has potential for wood production and medicinal purposes but it is threatened with extinction. This work investigated the callogenesis of the species and biochemically analyzed the calli produced, aiming at further studies of somatic embryogenesis. Leaf explants were inoculated in MS culture medium (Murashige and Skoog, 1962) supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (0.0, 2.5, 10.0 and 20.0 μM), kinetin (0.0, 2.5 and 5.0 μM) and glutamine (0.0, 0.34, 0.68 and 1.37 μM). For the growth curve, the fresh mass of explants with and without calli was determined until the 56th day of culture, at intervals of 7 days. Reducing sugars (RS), sucrose and total soluble sugars (TSS) were measured at each growth curve period. The use of 2,4-D was efficient for callus induction in Myracrodruon urundeuva, and callogenesis was enhanced with the combination of 2,4-D, KIN and glutamine in the nutrient medium. The growth curve of the calli presented sigmoidal shape with five distinct phases: lag, exponential, linear, deceleration and stationary. The content of sucrose and TSS presented different behavior according to the different stages of callus growth, exhibiting accumulation of sugars in the period of preparation for growth, degradation in the exponential phase and once again accumulated these carbohydrates in the final stages (deceleration and stationary). The RS content, except day zero, remained stable during all evaluated periods. The calli of Myracrodruon urundeuva have embryogenic potential, but further studies are suggested to obtain somatic embryos, as well as to identify the period in which they are formed.Myracrodruon urundeuva Fr. All. é uma árvore que pertence à família Anacardiaceae, conhecida popularmente como aroeira-do-sertão; possui potencial para utilização madeireira e medicinal, entretanto está ameaçada de extinção. Este trabalho objetivou estudar a calogênese da aroeira-do-sertão, bem como analisar bioquimicamente os calos produzidos, visando estudos posteriores em embriogênese somática. Explantes foliares foram inoculados em meio de cultura MS (MURASHIGE; SKOOG, 1962) suplementado com diferentes concentrações (0,0; 2,5; 5,0; 10,0 e 20,0 μM) de 2,4-D (2,4-diclorofenoxiacético), (0,0; 2,5 e 5,0 μM) CIN (cinetina) e (0,0; 0,34; 0,68 e 1,37 μM) glutamina. Para a curva de crescimento, pesou-se a massa fresca dos explantes com e sem calos até o 56º dia de cultivo, em intervalos de 7 dias. Foram determinados os açúcares redutores (AR), a sacarose e os açúcares solúveis totais (AST) em cada período da curva de crescimento. A utilização de 2,4-D é eficiente para a indução de calos em Myracrodruon urundeuva e a calogênese é potencializada com a combinação de 2,4-D, CIN e glutamina no meio nutritivo. A curva de crescimento dos calos de Myracrodruon urundeuva apresenta forma sigmoidal com cinco fases: lag, exponencial, linear, desaceleração e estacionária. O conteúdo de sacarose e AST apresentou comportamento distinto de acordo com as diferentes fases de crescimento dos calos, exibindo acúmulo de açúcares no período de preparação para o crescimento, degradação na fase exponencial e nova fase de acumulação nos estágios finais (desaceleração e estacionária). O conteúdo de AR, excetuando o dia “zero”, se manteve estável durante todos os períodos avaliados. Os calos de Myracrodruon urundeuva possuem potencial embriogênico, no entanto sugerem-se novos estudos para obtenção de embriões somáticos, bem como identificação do período em que os mesmos serão formados.Universidade Federal de Santa Maria2020-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsm.br/cienciaflorestal/article/view/3168910.5902/1980509831689Ciência Florestal; Vol. 30 No. 3 (2020); 700-717Ciência Florestal; v. 30 n. 3 (2020); 700-7171980-50980103-9954reponame:Ciência Florestal (Online)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/cienciaflorestal/article/view/31689/33576Copyright (c) 2020 Ciência Florestalinfo:eu-repo/semantics/openAccessSilva, Tecla dos SantosCarvalho Filho, Rosembrando Sosthenes LeiteTanan, Tamara TorresRocha, Thaylane CarneiroSantana, José Raniere Ferreira de2020-09-02T20:52:05Zoai:ojs.pkp.sfu.ca:article/31689Revistahttp://www.ufsm.br/cienciaflorestal/ONGhttps://old.scielo.br/oai/scielo-oai.php||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br1980-50980103-9954opendoar:2020-09-02T20:52:05Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Callogenesis in Myracrodruon urundeuva Fr. All. Calogênese em Myracrodruon urundeuva Fr. All. |
title |
Callogenesis in Myracrodruon urundeuva Fr. All. |
spellingShingle |
Callogenesis in Myracrodruon urundeuva Fr. All. Silva, Tecla dos Santos Aroeira-do-sertão Carbohydrates Medicinal plant In vitro culture Aroeira-do-sertão Carboidratos Planta medicinal Cultivo in vitro |
title_short |
Callogenesis in Myracrodruon urundeuva Fr. All. |
title_full |
Callogenesis in Myracrodruon urundeuva Fr. All. |
title_fullStr |
Callogenesis in Myracrodruon urundeuva Fr. All. |
title_full_unstemmed |
Callogenesis in Myracrodruon urundeuva Fr. All. |
title_sort |
Callogenesis in Myracrodruon urundeuva Fr. All. |
author |
Silva, Tecla dos Santos |
author_facet |
Silva, Tecla dos Santos Carvalho Filho, Rosembrando Sosthenes Leite Tanan, Tamara Torres Rocha, Thaylane Carneiro Santana, José Raniere Ferreira de |
author_role |
author |
author2 |
Carvalho Filho, Rosembrando Sosthenes Leite Tanan, Tamara Torres Rocha, Thaylane Carneiro Santana, José Raniere Ferreira de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Silva, Tecla dos Santos Carvalho Filho, Rosembrando Sosthenes Leite Tanan, Tamara Torres Rocha, Thaylane Carneiro Santana, José Raniere Ferreira de |
dc.subject.por.fl_str_mv |
Aroeira-do-sertão Carbohydrates Medicinal plant In vitro culture Aroeira-do-sertão Carboidratos Planta medicinal Cultivo in vitro |
topic |
Aroeira-do-sertão Carbohydrates Medicinal plant In vitro culture Aroeira-do-sertão Carboidratos Planta medicinal Cultivo in vitro |
description |
Myracrodruon urundeuva Fr. All.is a tree belonging to the family Anacardiaceae, popularly known as ‘aroeira-do-sertão’, which has potential for wood production and medicinal purposes but it is threatened with extinction. This work investigated the callogenesis of the species and biochemically analyzed the calli produced, aiming at further studies of somatic embryogenesis. Leaf explants were inoculated in MS culture medium (Murashige and Skoog, 1962) supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (0.0, 2.5, 10.0 and 20.0 μM), kinetin (0.0, 2.5 and 5.0 μM) and glutamine (0.0, 0.34, 0.68 and 1.37 μM). For the growth curve, the fresh mass of explants with and without calli was determined until the 56th day of culture, at intervals of 7 days. Reducing sugars (RS), sucrose and total soluble sugars (TSS) were measured at each growth curve period. The use of 2,4-D was efficient for callus induction in Myracrodruon urundeuva, and callogenesis was enhanced with the combination of 2,4-D, KIN and glutamine in the nutrient medium. The growth curve of the calli presented sigmoidal shape with five distinct phases: lag, exponential, linear, deceleration and stationary. The content of sucrose and TSS presented different behavior according to the different stages of callus growth, exhibiting accumulation of sugars in the period of preparation for growth, degradation in the exponential phase and once again accumulated these carbohydrates in the final stages (deceleration and stationary). The RS content, except day zero, remained stable during all evaluated periods. The calli of Myracrodruon urundeuva have embryogenic potential, but further studies are suggested to obtain somatic embryos, as well as to identify the period in which they are formed. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsm.br/cienciaflorestal/article/view/31689 10.5902/1980509831689 |
url |
https://periodicos.ufsm.br/cienciaflorestal/article/view/31689 |
identifier_str_mv |
10.5902/1980509831689 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.ufsm.br/cienciaflorestal/article/view/31689/33576 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Ciência Florestal info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Ciência Florestal |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Florestal; Vol. 30 No. 3 (2020); 700-717 Ciência Florestal; v. 30 n. 3 (2020); 700-717 1980-5098 0103-9954 reponame:Ciência Florestal (Online) instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Florestal (Online) |
collection |
Ciência Florestal (Online) |
repository.name.fl_str_mv |
Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br |
_version_ |
1799944133243043840 |