ENZYME ACTIVITIES FROM ACCELERATED AGING AND STORAGED SEEDS OF Eucalyptus grandis

• Autor(a) principal: Camargo, Maria Letícia Pereira de
• Data de Publicação: 2000
• Outros Autores: Mori, Edson Seizo, Mello, Eduardo José de, Oda, Shinitiro, Lima, Giuseppina Pacce
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Ciência Florestal (Online)
• Texto Completo: https://periodicos.ufsm.br/cienciaflorestal/article/view/485
• topic: accelerated aging; seeds; Eucalyptus grandis; enzyme activities.; envelhecimento; sementes; Eucalyptus grandis; atividade enzimática.

This research studied the standard procedure developed for crop seeds to examine the biochemical processes in accelerated aging seeds (kept for 96 hours at 42ºC and 100% RH) and stored seeds (from 5, 10 and 15 years) using isoenzyme electrophoresis  and  peroxidase  activity  by colorimetric assay. There was significant decrease (z-test) in  the  vigor of  accelerated  aging  seeds (54,9%) when compared to the stored ones (97,25%, 95,22% and 89,25%).The peroxidase (PER, EC 1.11.1.7) activity  was  low, however  there  was  na  increase  in activity with seed aging.There was a simultaneous increase in both malate dehidrogenase (MDH, EC 1.1.1.37) and esterase (EST, EC 3.1.1.1) activities. The results fllowed the in crease in the seeds storage period of time and of the stress which occurs due to the temperature and humidity conditions utilized in accelerated aging.