ENZYME ACTIVITIES FROM ACCELERATED AGING AND STORAGED SEEDS OF Eucalyptus grandis
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência Florestal (Online) |
Texto Completo: | https://periodicos.ufsm.br/cienciaflorestal/article/view/485 |
Resumo: | This research studied the standard procedure developed for crop seeds to examine the biochemical processes in accelerated aging seeds (kept for 96 hours at 42ºC and 100% RH) and stored seeds (from 5, 10 and 15 years) using isoenzyme electrophoresis and peroxidase activity by colorimetric assay. There was significant decrease (z-test) in the vigor of accelerated aging seeds (54,9%) when compared to the stored ones (97,25%, 95,22% and 89,25%).The peroxidase (PER, EC 1.11.1.7) activity was low, however there was na increase in activity with seed aging.There was a simultaneous increase in both malate dehidrogenase (MDH, EC 1.1.1.37) and esterase (EST, EC 3.1.1.1) activities. The results fllowed the in crease in the seeds storage period of time and of the stress which occurs due to the temperature and humidity conditions utilized in accelerated aging. |
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Ciência Florestal (Online) |
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ENZYME ACTIVITIES FROM ACCELERATED AGING AND STORAGED SEEDS OF Eucalyptus grandisAtividade enzimática em plântulas de Eucalyptus grandis provenientes de sementes envelhecidas artificialmente e naturalmente.accelerated agingseedsEucalyptus grandisenzyme activities.envelhecimentosementesEucalyptus grandisatividade enzimática.This research studied the standard procedure developed for crop seeds to examine the biochemical processes in accelerated aging seeds (kept for 96 hours at 42ºC and 100% RH) and stored seeds (from 5, 10 and 15 years) using isoenzyme electrophoresis and peroxidase activity by colorimetric assay. There was significant decrease (z-test) in the vigor of accelerated aging seeds (54,9%) when compared to the stored ones (97,25%, 95,22% and 89,25%).The peroxidase (PER, EC 1.11.1.7) activity was low, however there was na increase in activity with seed aging.There was a simultaneous increase in both malate dehidrogenase (MDH, EC 1.1.1.37) and esterase (EST, EC 3.1.1.1) activities. The results fllowed the in crease in the seeds storage period of time and of the stress which occurs due to the temperature and humidity conditions utilized in accelerated aging. O presente estudo empregou o procedimento de envelhecimento artificial, submetendo sementes por 96 horas à 42° C e 100% de UR para compará-las com sementes estocadas por 5, 10 e 15 anos e utilizou eletroforese de isoenzimas e atividade de peroxidase por método colorimétrico. Houve um decréscimo significante na germinação das sementes envelhecidas artificialmente, quando comparadas com as sementes estocadas. A atividade da peroxidase foi baixa, porém houve uma curva ascendente que acompanhou a idade das sementes. Observou-se um aumento nas atividades da malato desidrogenase e a-esterase 2 e 3 e uma diminuição acentuada para a-esterase 1 e baixa para a fosfatase ácida. Os resultados acompanharam o aumento do tempo de estocagem das sementes e do estresse que ocorre por causa das condições de temperatura e umidade empregadas no processo de envelhecimento artificial.Universidade Federal de Santa Maria2000-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsm.br/cienciaflorestal/article/view/48510.5902/19805098485Ciência Florestal; Vol. 10 No. 2 (2000); 113-122Ciência Florestal; v. 10 n. 2 (2000); 113-1221980-50980103-9954reponame:Ciência Florestal (Online)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/cienciaflorestal/article/view/485/371Camargo, Maria Letícia Pereira deMori, Edson SeizoMello, Eduardo José deOda, ShinitiroLima, Giuseppina Pacceinfo:eu-repo/semantics/openAccess2017-06-06T18:25:47Zoai:ojs.pkp.sfu.ca:article/485Revistahttp://www.ufsm.br/cienciaflorestal/ONGhttps://old.scielo.br/oai/scielo-oai.php||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br1980-50980103-9954opendoar:2017-06-06T18:25:47Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
ENZYME ACTIVITIES FROM ACCELERATED AGING AND STORAGED SEEDS OF Eucalyptus grandis Atividade enzimática em plântulas de Eucalyptus grandis provenientes de sementes envelhecidas artificialmente e naturalmente. |
title |
ENZYME ACTIVITIES FROM ACCELERATED AGING AND STORAGED SEEDS OF Eucalyptus grandis |
spellingShingle |
ENZYME ACTIVITIES FROM ACCELERATED AGING AND STORAGED SEEDS OF Eucalyptus grandis Camargo, Maria Letícia Pereira de accelerated aging seeds Eucalyptus grandis enzyme activities. envelhecimento sementes Eucalyptus grandis atividade enzimática. |
title_short |
ENZYME ACTIVITIES FROM ACCELERATED AGING AND STORAGED SEEDS OF Eucalyptus grandis |
title_full |
ENZYME ACTIVITIES FROM ACCELERATED AGING AND STORAGED SEEDS OF Eucalyptus grandis |
title_fullStr |
ENZYME ACTIVITIES FROM ACCELERATED AGING AND STORAGED SEEDS OF Eucalyptus grandis |
title_full_unstemmed |
ENZYME ACTIVITIES FROM ACCELERATED AGING AND STORAGED SEEDS OF Eucalyptus grandis |
title_sort |
ENZYME ACTIVITIES FROM ACCELERATED AGING AND STORAGED SEEDS OF Eucalyptus grandis |
author |
Camargo, Maria Letícia Pereira de |
author_facet |
Camargo, Maria Letícia Pereira de Mori, Edson Seizo Mello, Eduardo José de Oda, Shinitiro Lima, Giuseppina Pacce |
author_role |
author |
author2 |
Mori, Edson Seizo Mello, Eduardo José de Oda, Shinitiro Lima, Giuseppina Pacce |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Camargo, Maria Letícia Pereira de Mori, Edson Seizo Mello, Eduardo José de Oda, Shinitiro Lima, Giuseppina Pacce |
dc.subject.por.fl_str_mv |
accelerated aging seeds Eucalyptus grandis enzyme activities. envelhecimento sementes Eucalyptus grandis atividade enzimática. |
topic |
accelerated aging seeds Eucalyptus grandis enzyme activities. envelhecimento sementes Eucalyptus grandis atividade enzimática. |
description |
This research studied the standard procedure developed for crop seeds to examine the biochemical processes in accelerated aging seeds (kept for 96 hours at 42ºC and 100% RH) and stored seeds (from 5, 10 and 15 years) using isoenzyme electrophoresis and peroxidase activity by colorimetric assay. There was significant decrease (z-test) in the vigor of accelerated aging seeds (54,9%) when compared to the stored ones (97,25%, 95,22% and 89,25%).The peroxidase (PER, EC 1.11.1.7) activity was low, however there was na increase in activity with seed aging.There was a simultaneous increase in both malate dehidrogenase (MDH, EC 1.1.1.37) and esterase (EST, EC 3.1.1.1) activities. The results fllowed the in crease in the seeds storage period of time and of the stress which occurs due to the temperature and humidity conditions utilized in accelerated aging. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-06-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsm.br/cienciaflorestal/article/view/485 10.5902/19805098485 |
url |
https://periodicos.ufsm.br/cienciaflorestal/article/view/485 |
identifier_str_mv |
10.5902/19805098485 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.ufsm.br/cienciaflorestal/article/view/485/371 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Florestal; Vol. 10 No. 2 (2000); 113-122 Ciência Florestal; v. 10 n. 2 (2000); 113-122 1980-5098 0103-9954 reponame:Ciência Florestal (Online) instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Florestal (Online) |
collection |
Ciência Florestal (Online) |
repository.name.fl_str_mv |
Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br |
_version_ |
1799944125465755648 |