POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF Eucalyptus urograndis

Detalhes bibliográficos
Autor(a) principal: Lima, Giuseppina Pace Pereira
Data de Publicação: 2005
Outros Autores: Piza, Isabela M. Toledo, Henrique, Andréa, Takaki, Massanori
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência Florestal (Online)
Texto Completo: https://periodicos.ufsm.br/cienciaflorestal/article/view/1722
Resumo: Biochemical markers have been used for the analysis of plant cells submitted to several types of stress, among them salinity. This work aimed at analyzing the effect of saline stress in callus of Eucalyptus urograndis on polyamine contents. Explants (hypocotyls) obtained from seeds were inoculated in callus inductive medium, submitted to different levels of NaCl and analyzed at 10, 20 and 30 days after the inoculation. The free polyamines were extracted, isolated and quantified using TLC (Thin-Layer Chromatography). Putrescine content was higher and a fall in the spermidine content was observed in callus submitted to salinity condition. The results showed that polyamine accumulation is related to NaCl exposure in callus of Eucalyptus urograndis. The decrease in spermine content could be used as a biochemical marker for Eucalyptus callus subjected to salinity.
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spelling POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF Eucalyptus urograndisPolyamines as salinity biochemical marker in callus of Eucalyptus urograndis.micropropagationsalt stresspolyaminesEucalyptus.micropropagationsalt stresspolyaminesEucalyptusBiochemical markers have been used for the analysis of plant cells submitted to several types of stress, among them salinity. This work aimed at analyzing the effect of saline stress in callus of Eucalyptus urograndis on polyamine contents. Explants (hypocotyls) obtained from seeds were inoculated in callus inductive medium, submitted to different levels of NaCl and analyzed at 10, 20 and 30 days after the inoculation. The free polyamines were extracted, isolated and quantified using TLC (Thin-Layer Chromatography). Putrescine content was higher and a fall in the spermidine content was observed in callus submitted to salinity condition. The results showed that polyamine accumulation is related to NaCl exposure in callus of Eucalyptus urograndis. The decrease in spermine content could be used as a biochemical marker for Eucalyptus callus subjected to salinity.Biochemical markers have been used for the analysis of plant cells submitted to several types of stress, among them salinity. This work aimed at analyzing the effect of saline stress in callus of Eucalyptus urograndis on polyamine contents. Explants (hypocotyls) obtained from seeds were inoculated in callus inductive medium, submitted to different levels of NaCl and analyzed at 10, 20 and 30 days after the inoculation. The free polyamines were extracted, isolated and quantified using TLC (Thin-Layer Chromatography). Putrescine content was higher and a fall in the spermidine content was observed in callus submitted to salinity condition. The results showed that polyamine accumulation is related to NaCl exposure in callus of Eucalyptus urograndis. The decrease in spermine content could be used as a biochemical marker for Eucalyptus callus subjected to salinity.Universidade Federal de Santa Maria2005-03-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsm.br/cienciaflorestal/article/view/172210.5902/198050981722Ciência Florestal; Vol. 13 No. 1 (2003); 43-48Ciência Florestal; v. 13 n. 1 (2003); 43-481980-50980103-9954reponame:Ciência Florestal (Online)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/cienciaflorestal/article/view/1722/991Lima, Giuseppina Pace PereiraPiza, Isabela M. ToledoHenrique, AndréaTakaki, Massanoriinfo:eu-repo/semantics/openAccess2017-05-19T17:03:27Zoai:ojs.pkp.sfu.ca:article/1722Revistahttp://www.ufsm.br/cienciaflorestal/ONGhttps://old.scielo.br/oai/scielo-oai.php||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br1980-50980103-9954opendoar:2017-05-19T17:03:27Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF Eucalyptus urograndis
Polyamines as salinity biochemical marker in callus of Eucalyptus urograndis.
title POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF Eucalyptus urograndis
spellingShingle POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF Eucalyptus urograndis
Lima, Giuseppina Pace Pereira
micropropagation
salt stress
polyamines
Eucalyptus.
micropropagation
salt stress
polyamines
Eucalyptus
title_short POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF Eucalyptus urograndis
title_full POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF Eucalyptus urograndis
title_fullStr POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF Eucalyptus urograndis
title_full_unstemmed POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF Eucalyptus urograndis
title_sort POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF Eucalyptus urograndis
author Lima, Giuseppina Pace Pereira
author_facet Lima, Giuseppina Pace Pereira
Piza, Isabela M. Toledo
Henrique, Andréa
Takaki, Massanori
author_role author
author2 Piza, Isabela M. Toledo
Henrique, Andréa
Takaki, Massanori
author2_role author
author
author
dc.contributor.author.fl_str_mv Lima, Giuseppina Pace Pereira
Piza, Isabela M. Toledo
Henrique, Andréa
Takaki, Massanori
dc.subject.por.fl_str_mv micropropagation
salt stress
polyamines
Eucalyptus.
micropropagation
salt stress
polyamines
Eucalyptus
topic micropropagation
salt stress
polyamines
Eucalyptus.
micropropagation
salt stress
polyamines
Eucalyptus
description Biochemical markers have been used for the analysis of plant cells submitted to several types of stress, among them salinity. This work aimed at analyzing the effect of saline stress in callus of Eucalyptus urograndis on polyamine contents. Explants (hypocotyls) obtained from seeds were inoculated in callus inductive medium, submitted to different levels of NaCl and analyzed at 10, 20 and 30 days after the inoculation. The free polyamines were extracted, isolated and quantified using TLC (Thin-Layer Chromatography). Putrescine content was higher and a fall in the spermidine content was observed in callus submitted to salinity condition. The results showed that polyamine accumulation is related to NaCl exposure in callus of Eucalyptus urograndis. The decrease in spermine content could be used as a biochemical marker for Eucalyptus callus subjected to salinity.
publishDate 2005
dc.date.none.fl_str_mv 2005-03-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufsm.br/cienciaflorestal/article/view/1722
10.5902/198050981722
url https://periodicos.ufsm.br/cienciaflorestal/article/view/1722
identifier_str_mv 10.5902/198050981722
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.ufsm.br/cienciaflorestal/article/view/1722/991
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Florestal; Vol. 13 No. 1 (2003); 43-48
Ciência Florestal; v. 13 n. 1 (2003); 43-48
1980-5098
0103-9954
reponame:Ciência Florestal (Online)
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Florestal (Online)
collection Ciência Florestal (Online)
repository.name.fl_str_mv Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv ||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br
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