POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF Eucalyptus urograndis

Detalhes bibliográficos
Autor(a) principal: Lima, Giuseppina Pace Pereira [UNESP]
Data de Publicação: 2003
Outros Autores: Piza, Isabela M. Toledo [UNESP], Henrique, Andréa, Takaki, Massanori [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.5902/198050981722
http://hdl.handle.net/11449/213242
Resumo: Biochemical markers have been used for the analysis of plant cells submitted to several types of stress, among them salinity. This work aimed at analyzing the effect of saline stress in callus of Eucalyptus urograndis on polyamine contents. Explants (hypocotyls) obtained from seeds were inoculated in callus inductive medium, submitted to different levels of NaCl and analyzed at 10, 20 and 30 days after the inoculation. The free polyamines were extracted, isolated and quantified using TLC (Thin-Layer Chromatography). Putrescine content was higher and a fall in the spermidine content was observed in callus submitted to salinity condition. The results showed that polyamine accumulation is related to NaCl exposure in callus of Eucalyptus urograndis. The decrease in spermine content could be used as a biochemical marker for Eucalyptus callus subjected to salinity.
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spelling POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF Eucalyptus urograndisPOLIAMINAS COMO MARCADORES BIOQUÍMICOS DE SALINIDADE EM CALOS DE Eucalyptus urograndismicropropagationsalt stresspolyaminesEucalyptus.micropropagaçãoestresse salinopoliaminasEucalyptusBiochemical markers have been used for the analysis of plant cells submitted to several types of stress, among them salinity. This work aimed at analyzing the effect of saline stress in callus of Eucalyptus urograndis on polyamine contents. Explants (hypocotyls) obtained from seeds were inoculated in callus inductive medium, submitted to different levels of NaCl and analyzed at 10, 20 and 30 days after the inoculation. The free polyamines were extracted, isolated and quantified using TLC (Thin-Layer Chromatography). Putrescine content was higher and a fall in the spermidine content was observed in callus submitted to salinity condition. The results showed that polyamine accumulation is related to NaCl exposure in callus of Eucalyptus urograndis. The decrease in spermine content could be used as a biochemical marker for Eucalyptus callus subjected to salinity.Marcadores bioquímicos têm sido usados na análise de plantas submetidas a vários tipos de estresse, entre eles a salinidade. Este trabalho teve como objetivo analisar o estresse salino em calos de Eucalyptus urograndis em relação ao conteúdo de poliaminas. Explantes (hipocótilos) obtidos de sementes foram inoculados em meio indutivo de calos e foram submetidos a diferentes níveis de NaCl e analisados aos 10, 20 e 30 dias de inoculação. As poliaminas livres foram extraídas, isoladas e quantificadas usando TLC (Cromatografia de Camada Delgada). O conteúdo de putrescina foi maior, enquanto que o conteúdo de espermidina apresentou decréscimo, em calos submetidos a condições salinas. Os resultados mostram que o acúmulo de putrescina está relacionado com a exposição a NaCl em calos de Eucalyptus urograndis. A diminuição do conteúdo de espermina pode ser usada como marcador bioquímico de calos de Eucalyptus sujeitos à salinidade.Universidade Estadual Paulista, Instituto de BiociênciasAracruz Celulose S.A, Centro de Pesquisa e Tecnologia da Empresa Aracruz S.AUniversidade Estadual Paulista, Instituto de BiociênciasUniversidade Federal de Santa MariaUniversidade Estadual Paulista (Unesp)Aracruz Celulose S.ALima, Giuseppina Pace Pereira [UNESP]Piza, Isabela M. Toledo [UNESP]Henrique, AndréaTakaki, Massanori [UNESP]2021-07-14T10:52:10Z2021-07-14T10:52:10Z2003info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article43-48application/pdfhttp://dx.doi.org/10.5902/198050981722Ciência Florestal. Universidade Federal de Santa Maria, v. 13, n. 1, p. 43-48, 2003.0103-99541980-5098http://hdl.handle.net/11449/21324210.5902/198050981722S1980-50982003000100043S1980-50982003000100043.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCiência Florestalinfo:eu-repo/semantics/openAccess2023-12-11T06:15:28Zoai:repositorio.unesp.br:11449/213242Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-11T06:15:28Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF Eucalyptus urograndis
POLIAMINAS COMO MARCADORES BIOQUÍMICOS DE SALINIDADE EM CALOS DE Eucalyptus urograndis
title POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF Eucalyptus urograndis
spellingShingle POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF Eucalyptus urograndis
Lima, Giuseppina Pace Pereira [UNESP]
micropropagation
salt stress
polyamines
Eucalyptus.
micropropagação
estresse salino
poliaminas
Eucalyptus
title_short POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF Eucalyptus urograndis
title_full POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF Eucalyptus urograndis
title_fullStr POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF Eucalyptus urograndis
title_full_unstemmed POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF Eucalyptus urograndis
title_sort POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF Eucalyptus urograndis
author Lima, Giuseppina Pace Pereira [UNESP]
author_facet Lima, Giuseppina Pace Pereira [UNESP]
Piza, Isabela M. Toledo [UNESP]
Henrique, Andréa
Takaki, Massanori [UNESP]
author_role author
author2 Piza, Isabela M. Toledo [UNESP]
Henrique, Andréa
Takaki, Massanori [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Aracruz Celulose S.A
dc.contributor.author.fl_str_mv Lima, Giuseppina Pace Pereira [UNESP]
Piza, Isabela M. Toledo [UNESP]
Henrique, Andréa
Takaki, Massanori [UNESP]
dc.subject.por.fl_str_mv micropropagation
salt stress
polyamines
Eucalyptus.
micropropagação
estresse salino
poliaminas
Eucalyptus
topic micropropagation
salt stress
polyamines
Eucalyptus.
micropropagação
estresse salino
poliaminas
Eucalyptus
description Biochemical markers have been used for the analysis of plant cells submitted to several types of stress, among them salinity. This work aimed at analyzing the effect of saline stress in callus of Eucalyptus urograndis on polyamine contents. Explants (hypocotyls) obtained from seeds were inoculated in callus inductive medium, submitted to different levels of NaCl and analyzed at 10, 20 and 30 days after the inoculation. The free polyamines were extracted, isolated and quantified using TLC (Thin-Layer Chromatography). Putrescine content was higher and a fall in the spermidine content was observed in callus submitted to salinity condition. The results showed that polyamine accumulation is related to NaCl exposure in callus of Eucalyptus urograndis. The decrease in spermine content could be used as a biochemical marker for Eucalyptus callus subjected to salinity.
publishDate 2003
dc.date.none.fl_str_mv 2003
2021-07-14T10:52:10Z
2021-07-14T10:52:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.5902/198050981722
Ciência Florestal. Universidade Federal de Santa Maria, v. 13, n. 1, p. 43-48, 2003.
0103-9954
1980-5098
http://hdl.handle.net/11449/213242
10.5902/198050981722
S1980-50982003000100043
S1980-50982003000100043.pdf
url http://dx.doi.org/10.5902/198050981722
http://hdl.handle.net/11449/213242
identifier_str_mv Ciência Florestal. Universidade Federal de Santa Maria, v. 13, n. 1, p. 43-48, 2003.
0103-9954
1980-5098
10.5902/198050981722
S1980-50982003000100043
S1980-50982003000100043.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ciência Florestal
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 43-48
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1792962052113825792

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