Histomorphological and biochemical characterization of callus induction in Cenostigma pyramidale [Tul.] Gagnon & G.P. Lewis
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência Florestal (Online) |
Texto Completo: | https://periodicos.ufsm.br/cienciaflorestal/article/view/66334 |
Resumo: | Cenostigma pyramidale [Tul.] has timber, forage and medicinal uses, and due to the exploitation of its natural resources, it may become endangered. This work aimed to induce callus in Cenostigma pyramidale and to identify the embryogenic potential by histomorphological and biochemical analysis. Leaf segments were inoculated in MS culture medium containing different concentrations (0.0; 2.5; 5.0 and 10.0 μM) of 2,4-dichlorophenoxyacetic acid (2,4-D), combined with 6-benzylaminopurine (BAP; 0.0; 2.5 and 5.0 μM) and glutamine (0.0; 0.342 and 0.684 mM). The growth curve was performed at 7-day intervals until day 77, and samples were collected for histomorphological analysis and determination of reducing sugars (RA), sucrose, and total soluble sugars (TSS) content. Callus induction is possible using leaf segments in treatment with 5.0 μM of BAP, 7.0 µM of 2.4-D and 0.684 mM of glutamine. The growth curve shows sigmoidal behavior with five distinct phases: lag, exponential, linear, deceleration and decline. It was verified the presence of cells with meristematic characteristics from the 7th day of induction, suggesting that the calli have embryogenic potential. However, differentiation of somatic embryos was not observed. There was a reduction in RA content and increase in AST and sucrose during the lag phase and beginning of the exponential phase, followed by degradation in the exponential phase until the decline phase, showing the involvement of these carbohydrates with callus growth. These results are unprecedented for the species and suggest future studies related to callus maturation aiming the differentiation of somatic embryos. |
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Histomorphological and biochemical characterization of callus induction in Cenostigma pyramidale [Tul.] Gagnon & G.P. LewisCaracterização histomorfológica e bioquímica de calos induzidos em Cenostigma pyramidale [Tul.] Gagnon & G.P. LewisCultivo in vitroCurva de crescimentoCarboidratosIn vitro cultivationGrowth curveCarbohydratesCenostigma pyramidale [Tul.] has timber, forage and medicinal uses, and due to the exploitation of its natural resources, it may become endangered. This work aimed to induce callus in Cenostigma pyramidale and to identify the embryogenic potential by histomorphological and biochemical analysis. Leaf segments were inoculated in MS culture medium containing different concentrations (0.0; 2.5; 5.0 and 10.0 μM) of 2,4-dichlorophenoxyacetic acid (2,4-D), combined with 6-benzylaminopurine (BAP; 0.0; 2.5 and 5.0 μM) and glutamine (0.0; 0.342 and 0.684 mM). The growth curve was performed at 7-day intervals until day 77, and samples were collected for histomorphological analysis and determination of reducing sugars (RA), sucrose, and total soluble sugars (TSS) content. Callus induction is possible using leaf segments in treatment with 5.0 μM of BAP, 7.0 µM of 2.4-D and 0.684 mM of glutamine. The growth curve shows sigmoidal behavior with five distinct phases: lag, exponential, linear, deceleration and decline. It was verified the presence of cells with meristematic characteristics from the 7th day of induction, suggesting that the calli have embryogenic potential. However, differentiation of somatic embryos was not observed. There was a reduction in RA content and increase in AST and sucrose during the lag phase and beginning of the exponential phase, followed by degradation in the exponential phase until the decline phase, showing the involvement of these carbohydrates with callus growth. These results are unprecedented for the species and suggest future studies related to callus maturation aiming the differentiation of somatic embryos.Cenostigma pyramidale [Tul.] possui utilização madeireira, forrageira e medicinal e, em virtude da exploração de seus recursos naturais, pode acabar em via de extinção. Este trabalho objetivou induzir calos em Cenostigma pyramidale e identificar o potencial embriogênico por análises histomorfológica e bioquímica. Segmentos foliolares foram inoculados em meio de cultura MS contendo diferentes concentrações (0,0; 2,5; 5,0 e 10,0 μM) de ácido 2,4-diclorofenoxiacético (2,4-D), combinadas com 6-benzilaminopurina (BAP; 0,0; 2,5 e 5,0 μM) e glutamina (0,0; 0,342 e 0,684 mM). A curva de crescimento foi realizada em intervalos de 7 dias, até o 77º dia, sendo coletadas amostras para análise histomorfológica e determinação do conteúdo de açúcares redutores (AR), sacarose e açúcares solúveis totais (AST). É possível a indução de calos utilizando segmentos foliolares no tratamento com 5,0 μM de BAP, 7,0 µM de 2,4-D e 0,684 mM de glutamina. A curva de crescimento apresenta comportamento sigmoidal com cinco fases distintas: lag, exponencial, linear, desaceleração e declínio. Verificou-se a presença de células com características meristemáticas a partir do 7º dia de indução, sugerindo que os calos possuem potencial embriogênico. Porém, não se observou a diferenciação de embriões somáticos. Verificaram-se redução no conteúdo de AR e aumento de AST e sacarose durante a fase lag e início da fase exponencial, seguido de degradação na fase exponencial até a fase de declínio, mostrando o envolvimento destes carboidratos com o crescimento dos calos. Esses resultados são inéditos para a espécie e sugerem estudos futuros relacionados à maturação dos calos visando a diferenciação de embriões somáticos.Universidade Federal de Santa Maria2023-03-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsm.br/cienciaflorestal/article/view/6633410.5902/1980509866334Ciência Florestal; Vol. 33 No. 1 (2023): Publicação Contínua; e66334Ciência Florestal; v. 33 n. 1 (2023): Publicação Contínua; e663341980-50980103-9954reponame:Ciência Florestal (Online)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/cienciaflorestal/article/view/66334/51873Copyright (c) 2023 Ciência Florestalhttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessCarvalho Filho, Rosembrando Sosthenes LeiteSilva, Tecla dos SantosRibeiro, Yrexam Rodrigues de SouzaSanta-Catarina, ClaudeteSantana, José Raniere Ferreira de2023-06-07T17:41:02Zoai:ojs.pkp.sfu.ca:article/66334Revistahttp://www.ufsm.br/cienciaflorestal/ONGhttps://old.scielo.br/oai/scielo-oai.php||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br1980-50980103-9954opendoar:2023-06-07T17:41:02Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Histomorphological and biochemical characterization of callus induction in Cenostigma pyramidale [Tul.] Gagnon & G.P. Lewis Caracterização histomorfológica e bioquímica de calos induzidos em Cenostigma pyramidale [Tul.] Gagnon & G.P. Lewis |
title |
Histomorphological and biochemical characterization of callus induction in Cenostigma pyramidale [Tul.] Gagnon & G.P. Lewis |
spellingShingle |
Histomorphological and biochemical characterization of callus induction in Cenostigma pyramidale [Tul.] Gagnon & G.P. Lewis Carvalho Filho, Rosembrando Sosthenes Leite Cultivo in vitro Curva de crescimento Carboidratos In vitro cultivation Growth curve Carbohydrates |
title_short |
Histomorphological and biochemical characterization of callus induction in Cenostigma pyramidale [Tul.] Gagnon & G.P. Lewis |
title_full |
Histomorphological and biochemical characterization of callus induction in Cenostigma pyramidale [Tul.] Gagnon & G.P. Lewis |
title_fullStr |
Histomorphological and biochemical characterization of callus induction in Cenostigma pyramidale [Tul.] Gagnon & G.P. Lewis |
title_full_unstemmed |
Histomorphological and biochemical characterization of callus induction in Cenostigma pyramidale [Tul.] Gagnon & G.P. Lewis |
title_sort |
Histomorphological and biochemical characterization of callus induction in Cenostigma pyramidale [Tul.] Gagnon & G.P. Lewis |
author |
Carvalho Filho, Rosembrando Sosthenes Leite |
author_facet |
Carvalho Filho, Rosembrando Sosthenes Leite Silva, Tecla dos Santos Ribeiro, Yrexam Rodrigues de Souza Santa-Catarina, Claudete Santana, José Raniere Ferreira de |
author_role |
author |
author2 |
Silva, Tecla dos Santos Ribeiro, Yrexam Rodrigues de Souza Santa-Catarina, Claudete Santana, José Raniere Ferreira de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Carvalho Filho, Rosembrando Sosthenes Leite Silva, Tecla dos Santos Ribeiro, Yrexam Rodrigues de Souza Santa-Catarina, Claudete Santana, José Raniere Ferreira de |
dc.subject.por.fl_str_mv |
Cultivo in vitro Curva de crescimento Carboidratos In vitro cultivation Growth curve Carbohydrates |
topic |
Cultivo in vitro Curva de crescimento Carboidratos In vitro cultivation Growth curve Carbohydrates |
description |
Cenostigma pyramidale [Tul.] has timber, forage and medicinal uses, and due to the exploitation of its natural resources, it may become endangered. This work aimed to induce callus in Cenostigma pyramidale and to identify the embryogenic potential by histomorphological and biochemical analysis. Leaf segments were inoculated in MS culture medium containing different concentrations (0.0; 2.5; 5.0 and 10.0 μM) of 2,4-dichlorophenoxyacetic acid (2,4-D), combined with 6-benzylaminopurine (BAP; 0.0; 2.5 and 5.0 μM) and glutamine (0.0; 0.342 and 0.684 mM). The growth curve was performed at 7-day intervals until day 77, and samples were collected for histomorphological analysis and determination of reducing sugars (RA), sucrose, and total soluble sugars (TSS) content. Callus induction is possible using leaf segments in treatment with 5.0 μM of BAP, 7.0 µM of 2.4-D and 0.684 mM of glutamine. The growth curve shows sigmoidal behavior with five distinct phases: lag, exponential, linear, deceleration and decline. It was verified the presence of cells with meristematic characteristics from the 7th day of induction, suggesting that the calli have embryogenic potential. However, differentiation of somatic embryos was not observed. There was a reduction in RA content and increase in AST and sucrose during the lag phase and beginning of the exponential phase, followed by degradation in the exponential phase until the decline phase, showing the involvement of these carbohydrates with callus growth. These results are unprecedented for the species and suggest future studies related to callus maturation aiming the differentiation of somatic embryos. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-03-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsm.br/cienciaflorestal/article/view/66334 10.5902/1980509866334 |
url |
https://periodicos.ufsm.br/cienciaflorestal/article/view/66334 |
identifier_str_mv |
10.5902/1980509866334 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.ufsm.br/cienciaflorestal/article/view/66334/51873 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Ciência Florestal http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Ciência Florestal http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Florestal; Vol. 33 No. 1 (2023): Publicação Contínua; e66334 Ciência Florestal; v. 33 n. 1 (2023): Publicação Contínua; e66334 1980-5098 0103-9954 reponame:Ciência Florestal (Online) instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Florestal (Online) |
collection |
Ciência Florestal (Online) |
repository.name.fl_str_mv |
Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br |
_version_ |
1799944135960952832 |