Histomorphological and biochemical characterization of callus induction in Cenostigma pyramidale [Tul.] Gagnon & G.P. Lewis

Detalhes bibliográficos
Autor(a) principal: Carvalho Filho, Rosembrando Sosthenes Leite
Data de Publicação: 2023
Outros Autores: Silva, Tecla dos Santos, Ribeiro, Yrexam Rodrigues de Souza, Santa-Catarina, Claudete, Santana, José Raniere Ferreira de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência Florestal (Online)
Texto Completo: https://periodicos.ufsm.br/cienciaflorestal/article/view/66334
Resumo: Cenostigma pyramidale [Tul.] has timber, forage and medicinal uses, and due to the exploitation of its natural resources, it may become endangered. This work aimed to induce callus in Cenostigma pyramidale and to identify the embryogenic potential by histomorphological and biochemical analysis. Leaf segments were inoculated in MS culture medium containing different concentrations (0.0; 2.5; 5.0 and 10.0 μM) of 2,4-dichlorophenoxyacetic acid (2,4-D), combined with 6-benzylaminopurine (BAP; 0.0; 2.5 and 5.0 μM) and glutamine (0.0; 0.342 and 0.684 mM). The growth curve was performed at 7-day intervals until day 77, and samples were collected for histomorphological analysis and determination of reducing sugars (RA), sucrose, and total soluble sugars (TSS) content. Callus induction is possible using leaf segments in treatment with 5.0 μM of BAP, 7.0 µM of 2.4-D and 0.684 mM of glutamine. The growth curve shows sigmoidal behavior with five distinct phases: lag, exponential, linear, deceleration and decline. It was verified the presence of cells with meristematic characteristics from the 7th day of induction, suggesting that the calli have embryogenic potential. However, differentiation of somatic embryos was not observed. There was a reduction in RA content and increase in AST and sucrose during the lag phase and beginning of the exponential phase, followed by degradation in the exponential phase until the decline phase, showing the involvement of these carbohydrates with callus growth. These results are unprecedented for the species and suggest future studies related to callus maturation aiming the differentiation of somatic embryos.
id UFSM-6_d180517943a0cbf69dacff082d5d40de
oai_identifier_str oai:ojs.pkp.sfu.ca:article/66334
network_acronym_str UFSM-6
network_name_str Ciência Florestal (Online)
repository_id_str
spelling Histomorphological and biochemical characterization of callus induction in Cenostigma pyramidale [Tul.] Gagnon & G.P. LewisCaracterização histomorfológica e bioquímica de calos induzidos em Cenostigma pyramidale [Tul.] Gagnon & G.P. LewisCultivo in vitroCurva de crescimentoCarboidratosIn vitro cultivationGrowth curveCarbohydratesCenostigma pyramidale [Tul.] has timber, forage and medicinal uses, and due to the exploitation of its natural resources, it may become endangered. This work aimed to induce callus in Cenostigma pyramidale and to identify the embryogenic potential by histomorphological and biochemical analysis. Leaf segments were inoculated in MS culture medium containing different concentrations (0.0; 2.5; 5.0 and 10.0 μM) of 2,4-dichlorophenoxyacetic acid (2,4-D), combined with 6-benzylaminopurine (BAP; 0.0; 2.5 and 5.0 μM) and glutamine (0.0; 0.342 and 0.684 mM). The growth curve was performed at 7-day intervals until day 77, and samples were collected for histomorphological analysis and determination of reducing sugars (RA), sucrose, and total soluble sugars (TSS) content. Callus induction is possible using leaf segments in treatment with 5.0 μM of BAP, 7.0 µM of 2.4-D and 0.684 mM of glutamine. The growth curve shows sigmoidal behavior with five distinct phases: lag, exponential, linear, deceleration and decline. It was verified the presence of cells with meristematic characteristics from the 7th day of induction, suggesting that the calli have embryogenic potential. However, differentiation of somatic embryos was not observed. There was a reduction in RA content and increase in AST and sucrose during the lag phase and beginning of the exponential phase, followed by degradation in the exponential phase until the decline phase, showing the involvement of these carbohydrates with callus growth. These results are unprecedented for the species and suggest future studies related to callus maturation aiming the differentiation of somatic embryos.Cenostigma pyramidale [Tul.] possui utilização madeireira, forrageira e medicinal e, em virtude da exploração de seus recursos naturais, pode acabar em via de extinção. Este trabalho objetivou induzir calos em Cenostigma pyramidale e identificar o potencial embriogênico por análises histomorfológica e bioquímica. Segmentos foliolares foram inoculados em meio de cultura MS contendo diferentes concentrações (0,0; 2,5; 5,0 e 10,0 μM) de ácido 2,4-diclorofenoxiacético (2,4-D), combinadas com 6-benzilaminopurina (BAP; 0,0; 2,5 e 5,0 μM) e glutamina (0,0; 0,342 e 0,684 mM). A curva de crescimento foi realizada em intervalos de 7 dias, até o 77º dia, sendo coletadas amostras para análise histomorfológica e determinação do conteúdo de açúcares redutores (AR), sacarose e açúcares solúveis totais (AST). É possível a indução de calos utilizando segmentos foliolares no tratamento com 5,0 μM de BAP, 7,0 µM de 2,4-D e 0,684 mM de glutamina. A curva de crescimento apresenta comportamento sigmoidal com cinco fases distintas: lag, exponencial, linear, desaceleração e declínio. Verificou-se a presença de células com características meristemáticas a partir do 7º dia de indução, sugerindo que os calos possuem potencial embriogênico. Porém, não se observou a diferenciação de embriões somáticos. Verificaram-se redução no conteúdo de AR e aumento de AST e sacarose durante a fase lag e início da fase exponencial, seguido de degradação na fase exponencial até a fase de declínio, mostrando o envolvimento destes carboidratos com o crescimento dos calos. Esses resultados são inéditos para a espécie e sugerem estudos futuros relacionados à maturação dos calos visando a diferenciação de embriões somáticos.Universidade Federal de Santa Maria2023-03-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsm.br/cienciaflorestal/article/view/6633410.5902/1980509866334Ciência Florestal; Vol. 33 No. 1 (2023): Publicação Contínua; e66334Ciência Florestal; v. 33 n. 1 (2023): Publicação Contínua; e663341980-50980103-9954reponame:Ciência Florestal (Online)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/cienciaflorestal/article/view/66334/51873Copyright (c) 2023 Ciência Florestalhttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessCarvalho Filho, Rosembrando Sosthenes LeiteSilva, Tecla dos SantosRibeiro, Yrexam Rodrigues de SouzaSanta-Catarina, ClaudeteSantana, José Raniere Ferreira de2023-06-07T17:41:02Zoai:ojs.pkp.sfu.ca:article/66334Revistahttp://www.ufsm.br/cienciaflorestal/ONGhttps://old.scielo.br/oai/scielo-oai.php||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br1980-50980103-9954opendoar:2023-06-07T17:41:02Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Histomorphological and biochemical characterization of callus induction in Cenostigma pyramidale [Tul.] Gagnon & G.P. Lewis
Caracterização histomorfológica e bioquímica de calos induzidos em Cenostigma pyramidale [Tul.] Gagnon & G.P. Lewis
title Histomorphological and biochemical characterization of callus induction in Cenostigma pyramidale [Tul.] Gagnon & G.P. Lewis
spellingShingle Histomorphological and biochemical characterization of callus induction in Cenostigma pyramidale [Tul.] Gagnon & G.P. Lewis
Carvalho Filho, Rosembrando Sosthenes Leite
Cultivo in vitro
Curva de crescimento
Carboidratos
In vitro cultivation
Growth curve
Carbohydrates
title_short Histomorphological and biochemical characterization of callus induction in Cenostigma pyramidale [Tul.] Gagnon & G.P. Lewis
title_full Histomorphological and biochemical characterization of callus induction in Cenostigma pyramidale [Tul.] Gagnon & G.P. Lewis
title_fullStr Histomorphological and biochemical characterization of callus induction in Cenostigma pyramidale [Tul.] Gagnon & G.P. Lewis
title_full_unstemmed Histomorphological and biochemical characterization of callus induction in Cenostigma pyramidale [Tul.] Gagnon & G.P. Lewis
title_sort Histomorphological and biochemical characterization of callus induction in Cenostigma pyramidale [Tul.] Gagnon & G.P. Lewis
author Carvalho Filho, Rosembrando Sosthenes Leite
author_facet Carvalho Filho, Rosembrando Sosthenes Leite
Silva, Tecla dos Santos
Ribeiro, Yrexam Rodrigues de Souza
Santa-Catarina, Claudete
Santana, José Raniere Ferreira de
author_role author
author2 Silva, Tecla dos Santos
Ribeiro, Yrexam Rodrigues de Souza
Santa-Catarina, Claudete
Santana, José Raniere Ferreira de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Carvalho Filho, Rosembrando Sosthenes Leite
Silva, Tecla dos Santos
Ribeiro, Yrexam Rodrigues de Souza
Santa-Catarina, Claudete
Santana, José Raniere Ferreira de
dc.subject.por.fl_str_mv Cultivo in vitro
Curva de crescimento
Carboidratos
In vitro cultivation
Growth curve
Carbohydrates
topic Cultivo in vitro
Curva de crescimento
Carboidratos
In vitro cultivation
Growth curve
Carbohydrates
description Cenostigma pyramidale [Tul.] has timber, forage and medicinal uses, and due to the exploitation of its natural resources, it may become endangered. This work aimed to induce callus in Cenostigma pyramidale and to identify the embryogenic potential by histomorphological and biochemical analysis. Leaf segments were inoculated in MS culture medium containing different concentrations (0.0; 2.5; 5.0 and 10.0 μM) of 2,4-dichlorophenoxyacetic acid (2,4-D), combined with 6-benzylaminopurine (BAP; 0.0; 2.5 and 5.0 μM) and glutamine (0.0; 0.342 and 0.684 mM). The growth curve was performed at 7-day intervals until day 77, and samples were collected for histomorphological analysis and determination of reducing sugars (RA), sucrose, and total soluble sugars (TSS) content. Callus induction is possible using leaf segments in treatment with 5.0 μM of BAP, 7.0 µM of 2.4-D and 0.684 mM of glutamine. The growth curve shows sigmoidal behavior with five distinct phases: lag, exponential, linear, deceleration and decline. It was verified the presence of cells with meristematic characteristics from the 7th day of induction, suggesting that the calli have embryogenic potential. However, differentiation of somatic embryos was not observed. There was a reduction in RA content and increase in AST and sucrose during the lag phase and beginning of the exponential phase, followed by degradation in the exponential phase until the decline phase, showing the involvement of these carbohydrates with callus growth. These results are unprecedented for the species and suggest future studies related to callus maturation aiming the differentiation of somatic embryos.
publishDate 2023
dc.date.none.fl_str_mv 2023-03-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufsm.br/cienciaflorestal/article/view/66334
10.5902/1980509866334
url https://periodicos.ufsm.br/cienciaflorestal/article/view/66334
identifier_str_mv 10.5902/1980509866334
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.ufsm.br/cienciaflorestal/article/view/66334/51873
dc.rights.driver.fl_str_mv Copyright (c) 2023 Ciência Florestal
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Ciência Florestal
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Florestal; Vol. 33 No. 1 (2023): Publicação Contínua; e66334
Ciência Florestal; v. 33 n. 1 (2023): Publicação Contínua; e66334
1980-5098
0103-9954
reponame:Ciência Florestal (Online)
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Florestal (Online)
collection Ciência Florestal (Online)
repository.name.fl_str_mv Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv ||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br
_version_ 1799944135960952832