Análise fitoquímica e avaliação das atividades antioxidante e antimicrobiana de Maytenus ilicifolia (Mart. ex Reissek)
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
dARK ID: | ark:/26339/0013000001xqh |
Texto Completo: | http://repositorio.ufsm.br/handle/1/18027 |
Resumo: | Despite the World Health Organization recognize the relevance of medicinal plants use by population to treat some health conditions, in some plant species, leaves are prepared from different forms without any criteria and previous efficacy and safety evaluation of each infusions preparation. This is the case of Maytenus ilicifolia Mart. ex Reissek (Celastraceae), popularly known as Espinheira-Santa. Popularly, this specie is used as infusion prepared from fresh or dried leaves. Therefore, the aim of this study was to evaluate the existence of potential differences of M. ilicifolia fresh and dried leaves extracts on bioactive molecules composition, antioxidant capacity and antimicrobial activity against 12 bacteria strains. Moreover, the effect of M. ilicifolia extracts on reactive oxidative species (ROS) modulation of bacteria were also investigated. Hydroalcoholic extracts using fresh and dried M. ilicifolia leaves were obtained and the quantification of eight bioactive molecules was performed by HPLC-DAD protocols. Antioxidant capacity and antibacterial effect were also evaluated. The M. ilicifolia extract produced from dried leaves presented higher concentration of bioactive compounds (mainly quercetin and caffeic acid), as well as a more efficient antibacterial activity despite the antioxidant capacity to be higher in fresh leaves extract. Both extracts at different concentrations decreased ROS levels. As concomitant antibacterial and antioxidant activities were observed in M. ilicifolia extracts, the results suggest that decreasing of ROS levels could cause cytotoxicity by reductive stress or to act as inhibitory signaling of bacteria proliferation. Complementary studies need to be perform to corroborate this hypothesis. The results presented here could be relevant, since show important impact of leaves preparation on extraction of some chemical compounds and the biological activities of M. ilicifolia. |
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Análise fitoquímica e avaliação das atividades antioxidante e antimicrobiana de Maytenus ilicifolia (Mart. ex Reissek)Phytochemical analysis and evaluation of antioxidant and antimicrobial activities of Maytenus ilicifolia (Mart. ex Reissek)Maytenus ilicifoliaPlantas medicinaisAtividade antioxidanteAtividade antimicrobianaMaytenus ilicifoliaAntioxidant activityAntimicrobial activityMedicinal plantsCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICADespite the World Health Organization recognize the relevance of medicinal plants use by population to treat some health conditions, in some plant species, leaves are prepared from different forms without any criteria and previous efficacy and safety evaluation of each infusions preparation. This is the case of Maytenus ilicifolia Mart. ex Reissek (Celastraceae), popularly known as Espinheira-Santa. Popularly, this specie is used as infusion prepared from fresh or dried leaves. Therefore, the aim of this study was to evaluate the existence of potential differences of M. ilicifolia fresh and dried leaves extracts on bioactive molecules composition, antioxidant capacity and antimicrobial activity against 12 bacteria strains. Moreover, the effect of M. ilicifolia extracts on reactive oxidative species (ROS) modulation of bacteria were also investigated. Hydroalcoholic extracts using fresh and dried M. ilicifolia leaves were obtained and the quantification of eight bioactive molecules was performed by HPLC-DAD protocols. Antioxidant capacity and antibacterial effect were also evaluated. The M. ilicifolia extract produced from dried leaves presented higher concentration of bioactive compounds (mainly quercetin and caffeic acid), as well as a more efficient antibacterial activity despite the antioxidant capacity to be higher in fresh leaves extract. Both extracts at different concentrations decreased ROS levels. As concomitant antibacterial and antioxidant activities were observed in M. ilicifolia extracts, the results suggest that decreasing of ROS levels could cause cytotoxicity by reductive stress or to act as inhibitory signaling of bacteria proliferation. Complementary studies need to be perform to corroborate this hypothesis. The results presented here could be relevant, since show important impact of leaves preparation on extraction of some chemical compounds and the biological activities of M. ilicifolia.A Organização Mundial da Saúde reconhece a relevância do uso de plantas medicinais pela população para tratar algumas doenças, entretanto, em algumas espécies de plantas, ainda é comum o uso das folhas em diferentes preparações sem nenhum critério e avaliação prévia da eficácia e segurança de cada preparo de infusão. Este é o caso da Maytenus ilicifolia Mart. ex Reissek (Celastraceae) conhecida como Espinheira-santa. Popularmente, esta espécie é preparada na forma de infusão utilizando ou folhas frescas ou folhas secas. Assim o objetivo deste estudo foi avaliar a existência da potencial diferença dos extratos de folhas secas e frescas de M. ilicifolia quanto à composição de moléculas bioativas, capacidade antioxidante e atividade antimicrobiana frente 12 cepas bacterianas. Além disso, o efeito dos extratos da M. icifolia sobre a modulação das EROs nas bactérias também foi investigado. Foram obtidos extratos hidroalcoólicos usando folhas frescas e secas de M. ilicifolia e a quantificação de oito moléculas bioativas foi realizada através da técnica de HPLC-DAD. Os extratos produzidos com folhas secas de M. ilicifolia apresentaram a maior concentração de compostos bioativos (principalmente quercetina e ácido cafeico), bem como uma maior eficiente atividade antibacteriana, apesar de a capacidade antioxidante ser maior no extrato de folhas frescas. Ambos os extratos em diferentes concentrações reduziram os níveis de EROs. As atividades antibacteriana e antioxidante foram observadas concomitantemente nos extratos de M. ilicifolia. Os resultados sugerem que o decréscimo dos níveis de EROs pode causar citotoxicidade por estresse redutivo ou agir como inibidor na sinalização da proliferação das bactérias. Estudos complementares precisam ser realizados para corroborar esta hipótese. Os resultados aqui apresentados podem ser relevantes, uma vez que mostram um importante impacto na preparação das folhas sobre a extração de alguns compostos e na atividade biológica de M. ilicifolia.Universidade Federal de Santa MariaBrasilBioquímicaUFSMPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaCentro de Ciências Naturais e ExatasCruz, Ivana Beatrice Mânica dahttp://lattes.cnpq.br/3426369324110716Alves, Sydney Hartzhttp://lattes.cnpq.br/0330782478769631Oliveira, Raul Moreira2019-08-26T21:32:00Z2019-08-26T21:32:00Z2016-01-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/18027ark:/26339/0013000001xqhporAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-04-29T17:58:53Zoai:repositorio.ufsm.br:1/18027Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-04-29T17:58:53Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Análise fitoquímica e avaliação das atividades antioxidante e antimicrobiana de Maytenus ilicifolia (Mart. ex Reissek) Phytochemical analysis and evaluation of antioxidant and antimicrobial activities of Maytenus ilicifolia (Mart. ex Reissek) |
title |
Análise fitoquímica e avaliação das atividades antioxidante e antimicrobiana de Maytenus ilicifolia (Mart. ex Reissek) |
spellingShingle |
Análise fitoquímica e avaliação das atividades antioxidante e antimicrobiana de Maytenus ilicifolia (Mart. ex Reissek) Oliveira, Raul Moreira Maytenus ilicifolia Plantas medicinais Atividade antioxidante Atividade antimicrobiana Maytenus ilicifolia Antioxidant activity Antimicrobial activity Medicinal plants CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Análise fitoquímica e avaliação das atividades antioxidante e antimicrobiana de Maytenus ilicifolia (Mart. ex Reissek) |
title_full |
Análise fitoquímica e avaliação das atividades antioxidante e antimicrobiana de Maytenus ilicifolia (Mart. ex Reissek) |
title_fullStr |
Análise fitoquímica e avaliação das atividades antioxidante e antimicrobiana de Maytenus ilicifolia (Mart. ex Reissek) |
title_full_unstemmed |
Análise fitoquímica e avaliação das atividades antioxidante e antimicrobiana de Maytenus ilicifolia (Mart. ex Reissek) |
title_sort |
Análise fitoquímica e avaliação das atividades antioxidante e antimicrobiana de Maytenus ilicifolia (Mart. ex Reissek) |
author |
Oliveira, Raul Moreira |
author_facet |
Oliveira, Raul Moreira |
author_role |
author |
dc.contributor.none.fl_str_mv |
Cruz, Ivana Beatrice Mânica da http://lattes.cnpq.br/3426369324110716 Alves, Sydney Hartz http://lattes.cnpq.br/0330782478769631 |
dc.contributor.author.fl_str_mv |
Oliveira, Raul Moreira |
dc.subject.por.fl_str_mv |
Maytenus ilicifolia Plantas medicinais Atividade antioxidante Atividade antimicrobiana Maytenus ilicifolia Antioxidant activity Antimicrobial activity Medicinal plants CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
topic |
Maytenus ilicifolia Plantas medicinais Atividade antioxidante Atividade antimicrobiana Maytenus ilicifolia Antioxidant activity Antimicrobial activity Medicinal plants CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Despite the World Health Organization recognize the relevance of medicinal plants use by population to treat some health conditions, in some plant species, leaves are prepared from different forms without any criteria and previous efficacy and safety evaluation of each infusions preparation. This is the case of Maytenus ilicifolia Mart. ex Reissek (Celastraceae), popularly known as Espinheira-Santa. Popularly, this specie is used as infusion prepared from fresh or dried leaves. Therefore, the aim of this study was to evaluate the existence of potential differences of M. ilicifolia fresh and dried leaves extracts on bioactive molecules composition, antioxidant capacity and antimicrobial activity against 12 bacteria strains. Moreover, the effect of M. ilicifolia extracts on reactive oxidative species (ROS) modulation of bacteria were also investigated. Hydroalcoholic extracts using fresh and dried M. ilicifolia leaves were obtained and the quantification of eight bioactive molecules was performed by HPLC-DAD protocols. Antioxidant capacity and antibacterial effect were also evaluated. The M. ilicifolia extract produced from dried leaves presented higher concentration of bioactive compounds (mainly quercetin and caffeic acid), as well as a more efficient antibacterial activity despite the antioxidant capacity to be higher in fresh leaves extract. Both extracts at different concentrations decreased ROS levels. As concomitant antibacterial and antioxidant activities were observed in M. ilicifolia extracts, the results suggest that decreasing of ROS levels could cause cytotoxicity by reductive stress or to act as inhibitory signaling of bacteria proliferation. Complementary studies need to be perform to corroborate this hypothesis. The results presented here could be relevant, since show important impact of leaves preparation on extraction of some chemical compounds and the biological activities of M. ilicifolia. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-26 2019-08-26T21:32:00Z 2019-08-26T21:32:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/18027 |
dc.identifier.dark.fl_str_mv |
ark:/26339/0013000001xqh |
url |
http://repositorio.ufsm.br/handle/1/18027 |
identifier_str_mv |
ark:/26339/0013000001xqh |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica Centro de Ciências Naturais e Exatas |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica Centro de Ciências Naturais e Exatas |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1815172266552459264 |