Efeitos da rutina sobre a atividade da aceticolinesterase e sobre o comportamento em ratos expostos ao cádmio
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/11222 |
Resumo: | This study investigated the effect of administration of cadmium (Cd), Rutin (Rut), or both, on lipid peroxidation in different brain structures, memory, anxiety and activity of the enzyme acetylcholinesterase (AChE) (ex vivo, in vitro and kinetic). The rats received Cd (2 mg / kg) and Rut (40 and 80 mg / kg) by gavage for 21 administrations on alternate days. To the ex vivo study, the animals were divided into six groups (n = 10): saline/saline, saline/Rut40, saline/Rut80, Cd/saline, Cd/Rut40 and Cd/Rut80. The trials of AChE in vitro were performed at concentrations of 1, 5, 10, 25 and 50 mM of Rut in rat brain homogenate controls. The same concentrations were used for kinetic analysis of Electroforus electricus (E.e.AChE). Cd increased lipid peroxidation in all structures analyzed except in the striatum. Rut reversed this effect only in the hypothalamus and hippocampus. Cd caused a decrease in test step-down latency and administration of Rut (40 and 80 mg/kg) reversed this effect of Cd. Cd increased the number and the percent of entries in enclosed arms of plus-maze, suggesting an anxiogenic effect of Cd. Rut (40 and 80 mg/kg) reversed the anxiogenic effect of Cd. Rats that were exposed to Cd and/or treated with Rut showed no altered sensitivity to shock, nor the locomotor activity. The ex vivo activity of the enzyme AChE increased in the cortex and hypothalamus of rats that received only Cd. Rut reversed this increase in both doses. In vitro activity of the enzyme AChE was inhibited at all concentrations of synaptosome cortex, cerebellum, striatum, hippocampus and hypothalamus. The kinetic analysis of the interaction between the Rut and E.e.AChE showed a competitive inhibition. The findings show that treatment with Rut decreased oxidative damage to the membrane, prevented the increase in AChE activity, reversed the memory deficit and anxiety caused by Cd. Moreover, characterized Rut as an inhibitor of AChE, showing that this flavonoid may modulate cholinergic neurotransmission and, consequently, improve cognition. |
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Efeitos da rutina sobre a atividade da aceticolinesterase e sobre o comportamento em ratos expostos ao cádmioEffects of rutin on the activity of the acetylcholinesterase and on the behavior of rats exposed to cadmiumRutinaCádmioAcetilcolinesteraseMemóriaAnsiedadeEstresse oxidativoFlavonóidesRutinCadmiumAcetylcholinesteraseMemoryAnxietyOxidative stressFlavonoidsCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAThis study investigated the effect of administration of cadmium (Cd), Rutin (Rut), or both, on lipid peroxidation in different brain structures, memory, anxiety and activity of the enzyme acetylcholinesterase (AChE) (ex vivo, in vitro and kinetic). The rats received Cd (2 mg / kg) and Rut (40 and 80 mg / kg) by gavage for 21 administrations on alternate days. To the ex vivo study, the animals were divided into six groups (n = 10): saline/saline, saline/Rut40, saline/Rut80, Cd/saline, Cd/Rut40 and Cd/Rut80. The trials of AChE in vitro were performed at concentrations of 1, 5, 10, 25 and 50 mM of Rut in rat brain homogenate controls. The same concentrations were used for kinetic analysis of Electroforus electricus (E.e.AChE). Cd increased lipid peroxidation in all structures analyzed except in the striatum. Rut reversed this effect only in the hypothalamus and hippocampus. Cd caused a decrease in test step-down latency and administration of Rut (40 and 80 mg/kg) reversed this effect of Cd. Cd increased the number and the percent of entries in enclosed arms of plus-maze, suggesting an anxiogenic effect of Cd. Rut (40 and 80 mg/kg) reversed the anxiogenic effect of Cd. Rats that were exposed to Cd and/or treated with Rut showed no altered sensitivity to shock, nor the locomotor activity. The ex vivo activity of the enzyme AChE increased in the cortex and hypothalamus of rats that received only Cd. Rut reversed this increase in both doses. In vitro activity of the enzyme AChE was inhibited at all concentrations of synaptosome cortex, cerebellum, striatum, hippocampus and hypothalamus. The kinetic analysis of the interaction between the Rut and E.e.AChE showed a competitive inhibition. The findings show that treatment with Rut decreased oxidative damage to the membrane, prevented the increase in AChE activity, reversed the memory deficit and anxiety caused by Cd. Moreover, characterized Rut as an inhibitor of AChE, showing that this flavonoid may modulate cholinergic neurotransmission and, consequently, improve cognition.Conselho Nacional de Desenvolvimento Científico e TecnológicoO presente estudo investigou o efeito da administração de Cádmio (Cd), de Rutina (Rut) ou de ambos, na peroxidação lipídica em diferentes estruturas encefálicas, na memória e ansiedade e na atividade da acetilcolinesterase (AChE) (ex vivo, in vitro e cinética) em ratos. Os animais receberam Cd (2 mg / kg) e Rut (40 e 80 mg / kg) por gavagem diariamente por 21 administrações em dias alternados. Os ratos do estudo ex vivo, foram divididos em seis grupos (n = 10): salina/salina, salina/Rut40, salina/Rut80, Cd/salina, Cd/Rut40 e Cd/Rut 80. Os ensaios da AChE in vitro foram realizados nas concentrações de 1, 5, 10, 25 e 50 μM de Rut em homogeneizado de encéfalo de ratos controles. As mesmas concentrações foram utilizadas para a análise cinética de Electroforus electricus (E.e. AChE). O metal aumentou a peroxidação lipídica em todas as estruturas analisadas exceto no estriado. A Rut reverteu esse efeito somente no hipotálamo e hipocampo. Houve uma piora da memória no grupo que recebeu somente Cd, porém a Rut reverteu essa diminuição de memória tanto nas doses de 40 quanto na de 80 mg/kg. O Cd teve efeito ansiogênico e a Rut reverteu esse comportamento em ambas as doses. A sensibilidade ao choque e a atividade locomotora não foram alteradas nos ratos que foram expostos ao Cd e/ou tratados com Rut. A atividade ex vivo da enzima AChE aumentou no córtex e no hipotálamo dos ratos que receberam somente cádmio. A Rut reverteu esse aumento em ambas as doses. A atividade in vitro da enzima AChE foi inibida em todas as concentrações em sinaptossoma de córtex, no cerebelo, no estriado, no hipocampo e hipotálamo. A análise cinética da interação entre Rut e E.e.AChE caracterizou uma inibição competitiva. Os achados do presente estudo demonstram que o tratamento com Rut diminuiu os danos oxidativos à membrana, impediu o aumento da atividade da AChE, reverteu o déficit de memória e a ansiedade causados pelo Cd. Além disso, caracterizou o composto como um inibidor da AChE , demonstrando que este flavonóide pode modular a neurotransmissão colinérgica e, conseqüentemente, melhorar a cognição.Universidade Federal de Santa MariaBRBioquímicaUFSMPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaMorsch, Vera Mariahttp://lattes.cnpq.br/1519648219507868Mazzanti, Cinthia Melazzo Andradehttp://lattes.cnpq.br/2886709251370905Fachinetto, Roseleihttp://lattes.cnpq.br/7203076675431306Fiorenza, Amanda Maino2015-02-132015-02-132011-09-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfFIORENZA, Amanda Maino. Effects of rutin on the activity of the acetylcholinesterase and on the behavior of rats exposed to cadmium. 2011. 94 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2011.http://repositorio.ufsm.br/handle/1/11222porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-07-01T12:35:43Zoai:repositorio.ufsm.br:1/11222Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-07-01T12:35:43Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Efeitos da rutina sobre a atividade da aceticolinesterase e sobre o comportamento em ratos expostos ao cádmio Effects of rutin on the activity of the acetylcholinesterase and on the behavior of rats exposed to cadmium |
title |
Efeitos da rutina sobre a atividade da aceticolinesterase e sobre o comportamento em ratos expostos ao cádmio |
spellingShingle |
Efeitos da rutina sobre a atividade da aceticolinesterase e sobre o comportamento em ratos expostos ao cádmio Fiorenza, Amanda Maino Rutina Cádmio Acetilcolinesterase Memória Ansiedade Estresse oxidativo Flavonóides Rutin Cadmium Acetylcholinesterase Memory Anxiety Oxidative stress Flavonoids CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Efeitos da rutina sobre a atividade da aceticolinesterase e sobre o comportamento em ratos expostos ao cádmio |
title_full |
Efeitos da rutina sobre a atividade da aceticolinesterase e sobre o comportamento em ratos expostos ao cádmio |
title_fullStr |
Efeitos da rutina sobre a atividade da aceticolinesterase e sobre o comportamento em ratos expostos ao cádmio |
title_full_unstemmed |
Efeitos da rutina sobre a atividade da aceticolinesterase e sobre o comportamento em ratos expostos ao cádmio |
title_sort |
Efeitos da rutina sobre a atividade da aceticolinesterase e sobre o comportamento em ratos expostos ao cádmio |
author |
Fiorenza, Amanda Maino |
author_facet |
Fiorenza, Amanda Maino |
author_role |
author |
dc.contributor.none.fl_str_mv |
Morsch, Vera Maria http://lattes.cnpq.br/1519648219507868 Mazzanti, Cinthia Melazzo Andrade http://lattes.cnpq.br/2886709251370905 Fachinetto, Roselei http://lattes.cnpq.br/7203076675431306 |
dc.contributor.author.fl_str_mv |
Fiorenza, Amanda Maino |
dc.subject.por.fl_str_mv |
Rutina Cádmio Acetilcolinesterase Memória Ansiedade Estresse oxidativo Flavonóides Rutin Cadmium Acetylcholinesterase Memory Anxiety Oxidative stress Flavonoids CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
topic |
Rutina Cádmio Acetilcolinesterase Memória Ansiedade Estresse oxidativo Flavonóides Rutin Cadmium Acetylcholinesterase Memory Anxiety Oxidative stress Flavonoids CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
This study investigated the effect of administration of cadmium (Cd), Rutin (Rut), or both, on lipid peroxidation in different brain structures, memory, anxiety and activity of the enzyme acetylcholinesterase (AChE) (ex vivo, in vitro and kinetic). The rats received Cd (2 mg / kg) and Rut (40 and 80 mg / kg) by gavage for 21 administrations on alternate days. To the ex vivo study, the animals were divided into six groups (n = 10): saline/saline, saline/Rut40, saline/Rut80, Cd/saline, Cd/Rut40 and Cd/Rut80. The trials of AChE in vitro were performed at concentrations of 1, 5, 10, 25 and 50 mM of Rut in rat brain homogenate controls. The same concentrations were used for kinetic analysis of Electroforus electricus (E.e.AChE). Cd increased lipid peroxidation in all structures analyzed except in the striatum. Rut reversed this effect only in the hypothalamus and hippocampus. Cd caused a decrease in test step-down latency and administration of Rut (40 and 80 mg/kg) reversed this effect of Cd. Cd increased the number and the percent of entries in enclosed arms of plus-maze, suggesting an anxiogenic effect of Cd. Rut (40 and 80 mg/kg) reversed the anxiogenic effect of Cd. Rats that were exposed to Cd and/or treated with Rut showed no altered sensitivity to shock, nor the locomotor activity. The ex vivo activity of the enzyme AChE increased in the cortex and hypothalamus of rats that received only Cd. Rut reversed this increase in both doses. In vitro activity of the enzyme AChE was inhibited at all concentrations of synaptosome cortex, cerebellum, striatum, hippocampus and hypothalamus. The kinetic analysis of the interaction between the Rut and E.e.AChE showed a competitive inhibition. The findings show that treatment with Rut decreased oxidative damage to the membrane, prevented the increase in AChE activity, reversed the memory deficit and anxiety caused by Cd. Moreover, characterized Rut as an inhibitor of AChE, showing that this flavonoid may modulate cholinergic neurotransmission and, consequently, improve cognition. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-09-09 2015-02-13 2015-02-13 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
FIORENZA, Amanda Maino. Effects of rutin on the activity of the acetylcholinesterase and on the behavior of rats exposed to cadmium. 2011. 94 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2011. http://repositorio.ufsm.br/handle/1/11222 |
identifier_str_mv |
FIORENZA, Amanda Maino. Effects of rutin on the activity of the acetylcholinesterase and on the behavior of rats exposed to cadmium. 2011. 94 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2011. |
url |
http://repositorio.ufsm.br/handle/1/11222 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922129883955200 |