Avaliação da atividade de ectonucleotidases no córtex cerebral e plaquetas de ratos expostos à fumaça de cigarro

Detalhes bibliográficos
Autor(a) principal: Thomé, Gustavo Roberto
Data de Publicação: 2009
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/001300000td5g
Texto Completo: http://repositorio.ufsm.br/handle/1/11108
Resumo: Inflammatory and degenerative pathophysiological processes within the central nervous system (CNS) and platelets are important causes of human disease promoted by cigarette smoke. The objective of the present study was to evaluate activity of the enzymes that hydrolyze nucleotides and nucleosides in synaptosomes of cerebral cortex and platelets from rats exposed to aged and sidestream diluted smoke of commercial cigarettes. The animals were randomly divided into two groups (ten rats in each group): control (not exposed to cigarette smoke) and smoke (exposed to cigarette smoke). The smoke group was exposed via inhalation through the nose only 15 minutes per day inside a cigarette smoke exposure chamber during 3 weeks. The results demonstrated that E-NTPDase (with ATP and ADP as substrate) activity was significantly higher in the smoke group (p<0.05), while the 5'- nucleotidase with AMP as substrate demonstrated decreased activity in the smoke group (p<0.05). However, in platelets activities demonstrated that E-NPP and ENTPDase with ATP as substrate and 5´-nucleotidase with AMP as substrate were significantly higher in the smoke group (p<0.05) as compared to the control group, while E-NTPDase with ADP as substrate demonstrated decreased activity in the smoke group (p<0.05). A decrease of adenosine, a neuroprotective nucleoside, was observed in brain, which could lead to damage of the brain tissue. However, in platelets, there was an increase of adenosine, which may reflect adaptative changes. These alterations caused by cigarette smoke on ectonucleotidase activities may assist in verifying pathophysiological effects in biological systems.
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spelling Avaliação da atividade de ectonucleotidases no córtex cerebral e plaquetas de ratos expostos à fumaça de cigarroE-NTPDase5'-nucleotidaseE-NPPPlaquetasSinaptosomasFumaça de cigarroPlateletsSynaptosomesCigarette smokeCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAInflammatory and degenerative pathophysiological processes within the central nervous system (CNS) and platelets are important causes of human disease promoted by cigarette smoke. The objective of the present study was to evaluate activity of the enzymes that hydrolyze nucleotides and nucleosides in synaptosomes of cerebral cortex and platelets from rats exposed to aged and sidestream diluted smoke of commercial cigarettes. The animals were randomly divided into two groups (ten rats in each group): control (not exposed to cigarette smoke) and smoke (exposed to cigarette smoke). The smoke group was exposed via inhalation through the nose only 15 minutes per day inside a cigarette smoke exposure chamber during 3 weeks. The results demonstrated that E-NTPDase (with ATP and ADP as substrate) activity was significantly higher in the smoke group (p<0.05), while the 5'- nucleotidase with AMP as substrate demonstrated decreased activity in the smoke group (p<0.05). However, in platelets activities demonstrated that E-NPP and ENTPDase with ATP as substrate and 5´-nucleotidase with AMP as substrate were significantly higher in the smoke group (p<0.05) as compared to the control group, while E-NTPDase with ADP as substrate demonstrated decreased activity in the smoke group (p<0.05). A decrease of adenosine, a neuroprotective nucleoside, was observed in brain, which could lead to damage of the brain tissue. However, in platelets, there was an increase of adenosine, which may reflect adaptative changes. These alterations caused by cigarette smoke on ectonucleotidase activities may assist in verifying pathophysiological effects in biological systems.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorOs processos patofisiológicos inflamatórios e degenerativos no sistema nervoso central (SNC) e plaquetas são importantes causas de doenças humanas promovidas pela fumaça de cigarro. O objetivo do presente estudo foi avaliar a atividade das enzimas que hidrolisam nucleotídeos e nucleosídeos em sinaptossomas de córtex cerebral e plaquetas de ratos expostos à fumaça envelhecida e diluída inalada por via secundária de cigarros comerciais. Os animais foram aleatoriamente divididos em dois grupos (10 ratos por grupo): controle (não exposto a fumaça de cigarro) e fumo (exposto a fumaça de cigarro). O grupo fumo foi exposto à fumaça de cigarro 15 minutos por dia no interior de uma câmara de exposição durante 3 semanas. Em relação ao sinaptossoma de córtex cerebral os resultados demonstraram que a atividade da E-NTPDase (ATP e ADP como substrato) aumentou significativamente no grupo exposto à fumaça de cigarro (p<0.05) e a atividade da 5'-nucleotidase (AMP com substrato) diminuiu neste grupo (p<0.05), comparado ao grupo controle. Nas plaquetas foi demonstrado um aumento na atividade das enzimas E-NPP e ENTPDase (ATP como substrato) e 5 -nucleotidase (AMP como substrato) no grupo exposto à fumaça de cigarro (p<0.05) quando comparado ao grupo controle. Já a ENTPDase (ADP como substrato) demonstrou uma diminuição de sua atividade no grupo exposto à fumaça de cigarro (p<0.05). Pode-se sugerir que a provável diminuição da adenosina, uma molécula neuroprotetora, pode levar ao dano no tecido do encéfalo. Entretanto, em plaquetas, houve um aumento da adenosina, a qual pode refletir mudanças adaptativas. As alterações na atividade das ectonucleotidases em animais submetidos à exposição de fumaça de cigarro podem contribuir na elucidação de seus efeitos patológicos em sistemas biológicos.Universidade Federal de Santa MariaBRBioquímicaUFSMPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaMazzanti, Cinthia Melazzo de Andradehttp://lattes.cnpq.br/2886709251370905Schetinger, Maria Rosa ChitolinaMoretto, Maria Beatrizhttp://lattes.cnpq.br/7317262818918502Rubin, Maribel Antonellohttp://lattes.cnpq.br/7237734243628134Thomé, Gustavo Roberto2017-04-242017-04-242009-03-31info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfTHOMÉ, Gustavo Roberto. Avaliação da atividade de ectonucleotidases no córtex cerebral e plaquetas de ratos expostos à fumaça de cigarro. 2009. 92 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2009.http://repositorio.ufsm.br/handle/1/11108ark:/26339/001300000td5gporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2023-05-02T13:01:52Zoai:repositorio.ufsm.br:1/11108Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-05-02T13:01:52Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Avaliação da atividade de ectonucleotidases no córtex cerebral e plaquetas de ratos expostos à fumaça de cigarro
title Avaliação da atividade de ectonucleotidases no córtex cerebral e plaquetas de ratos expostos à fumaça de cigarro
spellingShingle Avaliação da atividade de ectonucleotidases no córtex cerebral e plaquetas de ratos expostos à fumaça de cigarro
Thomé, Gustavo Roberto
E-NTPDase
5'-nucleotidase
E-NPP
Plaquetas
Sinaptosomas
Fumaça de cigarro
Platelets
Synaptosomes
Cigarette smoke
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
title_short Avaliação da atividade de ectonucleotidases no córtex cerebral e plaquetas de ratos expostos à fumaça de cigarro
title_full Avaliação da atividade de ectonucleotidases no córtex cerebral e plaquetas de ratos expostos à fumaça de cigarro
title_fullStr Avaliação da atividade de ectonucleotidases no córtex cerebral e plaquetas de ratos expostos à fumaça de cigarro
title_full_unstemmed Avaliação da atividade de ectonucleotidases no córtex cerebral e plaquetas de ratos expostos à fumaça de cigarro
title_sort Avaliação da atividade de ectonucleotidases no córtex cerebral e plaquetas de ratos expostos à fumaça de cigarro
author Thomé, Gustavo Roberto
author_facet Thomé, Gustavo Roberto
author_role author
dc.contributor.none.fl_str_mv Mazzanti, Cinthia Melazzo de Andrade
http://lattes.cnpq.br/2886709251370905
Schetinger, Maria Rosa Chitolina
Moretto, Maria Beatriz
http://lattes.cnpq.br/7317262818918502
Rubin, Maribel Antonello
http://lattes.cnpq.br/7237734243628134
dc.contributor.author.fl_str_mv Thomé, Gustavo Roberto
dc.subject.por.fl_str_mv E-NTPDase
5'-nucleotidase
E-NPP
Plaquetas
Sinaptosomas
Fumaça de cigarro
Platelets
Synaptosomes
Cigarette smoke
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
topic E-NTPDase
5'-nucleotidase
E-NPP
Plaquetas
Sinaptosomas
Fumaça de cigarro
Platelets
Synaptosomes
Cigarette smoke
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
description Inflammatory and degenerative pathophysiological processes within the central nervous system (CNS) and platelets are important causes of human disease promoted by cigarette smoke. The objective of the present study was to evaluate activity of the enzymes that hydrolyze nucleotides and nucleosides in synaptosomes of cerebral cortex and platelets from rats exposed to aged and sidestream diluted smoke of commercial cigarettes. The animals were randomly divided into two groups (ten rats in each group): control (not exposed to cigarette smoke) and smoke (exposed to cigarette smoke). The smoke group was exposed via inhalation through the nose only 15 minutes per day inside a cigarette smoke exposure chamber during 3 weeks. The results demonstrated that E-NTPDase (with ATP and ADP as substrate) activity was significantly higher in the smoke group (p<0.05), while the 5'- nucleotidase with AMP as substrate demonstrated decreased activity in the smoke group (p<0.05). However, in platelets activities demonstrated that E-NPP and ENTPDase with ATP as substrate and 5´-nucleotidase with AMP as substrate were significantly higher in the smoke group (p<0.05) as compared to the control group, while E-NTPDase with ADP as substrate demonstrated decreased activity in the smoke group (p<0.05). A decrease of adenosine, a neuroprotective nucleoside, was observed in brain, which could lead to damage of the brain tissue. However, in platelets, there was an increase of adenosine, which may reflect adaptative changes. These alterations caused by cigarette smoke on ectonucleotidase activities may assist in verifying pathophysiological effects in biological systems.
publishDate 2009
dc.date.none.fl_str_mv 2009-03-31
2017-04-24
2017-04-24
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv THOMÉ, Gustavo Roberto. Avaliação da atividade de ectonucleotidases no córtex cerebral e plaquetas de ratos expostos à fumaça de cigarro. 2009. 92 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2009.
http://repositorio.ufsm.br/handle/1/11108
dc.identifier.dark.fl_str_mv ark:/26339/001300000td5g
identifier_str_mv THOMÉ, Gustavo Roberto. Avaliação da atividade de ectonucleotidases no córtex cerebral e plaquetas de ratos expostos à fumaça de cigarro. 2009. 92 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2009.
ark:/26339/001300000td5g
url http://repositorio.ufsm.br/handle/1/11108
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Bioquímica
UFSM
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Bioquímica
UFSM
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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