Perfil da atividade de ectonucleotidases em plaquetas e agregação plaquetária em pacientes com lúpus eritematosos sistêmico
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/11128 |
Resumo: | Systemic Lupus Erythematosus (SLE) is a chronic inflammatory disease that affects mostly women in reproductive age and sometimes cause permanent damage. The development of cardiovascular disease, especially atherosclerosis, is the leading cause of death in SLE. However, its etiology and relationship with the development of atherosclerosis remain unknown. Several studies have shown that platelets have relevant properties to thrombogenesis, such as the release of ADP, a molecule capable of inducing platelet aggregation. Adenosine, derived from the hydrolysis of ATP and ADP, in turn has antiagreggant properties. The control of extracellular levels theses molecules and subsequent purinergic signaling induced by them is carried out by enzymes NTPDase, E-NPP, 5'-nucleotidase and ADA. Therefore, the objective of this study was to evaluate the activity of these ectonucleotidases in platelets and platelet aggregation profile in patients with LES. An increase in the activity of the enzymes NTPDase, E-NPP, 5'-nucleotidase and ADA was observed in platelets of patients with lupus compared with control subjects. No difference on platelet aggregation of patients with lupus was observed when compared to control. The increase in activity of E-NTPDase, E-NPP and 5'-nucleotidase seems to be a compensatory organic response against the pathological condition, to generate higher concentration of adenosine. But the ADA activity also is increased in platelets of patients with SLE and may decrease the concentration of adenosine, which favors prothrombotics process. Thus, the results suggest that the ectoenzymes may be involved in the modulation of atherosclerotic processes that occur in SLE. |
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2017-04-202017-04-202010-05-28ROSA, Cíntia Saydelles da. Profilet of the ectonucleotidase activity and platelets agreggation in systemic erythematosus lupus patients. 2010. 74 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2010.http://repositorio.ufsm.br/handle/1/11128Systemic Lupus Erythematosus (SLE) is a chronic inflammatory disease that affects mostly women in reproductive age and sometimes cause permanent damage. The development of cardiovascular disease, especially atherosclerosis, is the leading cause of death in SLE. However, its etiology and relationship with the development of atherosclerosis remain unknown. Several studies have shown that platelets have relevant properties to thrombogenesis, such as the release of ADP, a molecule capable of inducing platelet aggregation. Adenosine, derived from the hydrolysis of ATP and ADP, in turn has antiagreggant properties. The control of extracellular levels theses molecules and subsequent purinergic signaling induced by them is carried out by enzymes NTPDase, E-NPP, 5'-nucleotidase and ADA. Therefore, the objective of this study was to evaluate the activity of these ectonucleotidases in platelets and platelet aggregation profile in patients with LES. An increase in the activity of the enzymes NTPDase, E-NPP, 5'-nucleotidase and ADA was observed in platelets of patients with lupus compared with control subjects. No difference on platelet aggregation of patients with lupus was observed when compared to control. The increase in activity of E-NTPDase, E-NPP and 5'-nucleotidase seems to be a compensatory organic response against the pathological condition, to generate higher concentration of adenosine. But the ADA activity also is increased in platelets of patients with SLE and may decrease the concentration of adenosine, which favors prothrombotics process. Thus, the results suggest that the ectoenzymes may be involved in the modulation of atherosclerotic processes that occur in SLE.O lúpus Eritematoso Sistêmico é uma doença crônica, inflamatória e sistêmica, que afeta principalmente mulheres em idade reprodutiva e desencadeia danos permanentes na maioria dos casos. O desenvolvimento de doenças cardiovasculares, especialmente aterosclerose, é a principal causa de morte em pacientes lúpicos. Entretanto sua etiologia e relação com o desenvolvimento de aterosclerose permanecem desconhecidos. Vários estudos demonstraram que as plaquetas apresentam propriedades relevantes na trombogênese, como a liberação de ADP, uma molécula capaz de induzir a agregação plaquetária. A adenosina, proveniente da hidrólise do ATP e ADP, por sua vez apresenta propriedades antiagregantes. O controle dos níveis extracelulares destas moléculas e a consequente sinalização purinérgica por elas induzidas é realizado pelas enzimas NTPDase, E-NPP, 5'-nucleotidase e ADA. Sendo assim, o objetivo deste trabalho foi avaliar a atividade destas ectonucleotidases em plaquetas bem como o perfil da agregação plaquetária em pacientes lúpicos. Um aumento na atividade das enzimas NTPDase, E-NPP, 5'-nucleotidase e ADA foi observado em plaquetas de pacientes com LES, quando comparado com sujeitos controles. Nenhuma diferença no perfil de agregação plaquetária de pacientes lúpicas foi observada, quando comparado ao controle. O aumento na atividade da NTPDase, da E-NPP e da 5'-nucleotidase parece ser uma resposta compensatória orgânica frente ao estado patológico para gerar maior concentração de adenosina. Porém a atividade da ADA também encontra-se aumentada em plaquetas de pacientes com diagnóstico de LES, podendo diminuir a concentração de adenosina, o que favorece estágios prótrombóticos. Desse modo, os resultados encontrados sugerem que as ectoenzimas podem estar envolvidas na modulação dos processos ateroscleróticos que ocorrem no LES.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBRBioquímicaLESAterosclerosePlaquetasNTPDaseE-NPPADAAgregação plaquetáriaSystemic Lupus ErythematosusAtherosclerosisPlateletsENPPPlatelets aggregationCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAPerfil da atividade de ectonucleotidases em plaquetas e agregação plaquetária em pacientes com lúpus eritematosos sistêmicoProfilet of the ectonucleotidase activity and platelets agreggation in systemic erythematosus lupus patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisMorsch, Vera Maria Melchiorshttp://lattes.cnpq.br/1519648219507868Schetinger, Maria Rosa ChitolinaMazzanti, Cinthia Melazzo Andradehttp://lattes.cnpq.br/2886709251370905Fachinetto, Roseleihttp://lattes.cnpq.br/7203076675431306http://lattes.cnpq.br/8680932856088474Rosa, Cíntia Saydelles da20080000000240050030050050050005cf99a1-4e49-4daa-bb3b-26eee86569c125c468c6-1529-4ecc-9f9a-02c83f90c188e31536a8-f5a8-4f54-b813-f9fc8b2e268643340bb0-5546-46c4-9f5b-a11b7c95aace6a615e4f-285a-4d77-aeb6-a33cd9c07f80info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALROSA, CINTIA SAYDELLES DA.pdfapplication/pdf1899709http://repositorio.ufsm.br/bitstream/1/11128/1/ROSA%2c%20CINTIA%20SAYDELLES%20DA.pdf48b5c07ac4ae05f217030287b85b5dc8MD51TEXTROSA, CINTIA SAYDELLES DA.pdf.txtROSA, CINTIA SAYDELLES DA.pdf.txtExtracted texttext/plain108472http://repositorio.ufsm.br/bitstream/1/11128/2/ROSA%2c%20CINTIA%20SAYDELLES%20DA.pdf.txtc887778bfdb2b7f63c1aa7536e1fc25bMD52THUMBNAILROSA, CINTIA SAYDELLES DA.pdf.jpgROSA, CINTIA SAYDELLES DA.pdf.jpgIM Thumbnailimage/jpeg6740http://repositorio.ufsm.br/bitstream/1/11128/3/ROSA%2c%20CINTIA%20SAYDELLES%20DA.pdf.jpg2a581bf3d64f0f4d96c09a8e56e7336eMD531/111282022-09-13 15:46:13.239oai:repositorio.ufsm.br:1/11128Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-09-13T18:46:13Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Perfil da atividade de ectonucleotidases em plaquetas e agregação plaquetária em pacientes com lúpus eritematosos sistêmico |
dc.title.alternative.eng.fl_str_mv |
Profilet of the ectonucleotidase activity and platelets agreggation in systemic erythematosus lupus patients |
title |
Perfil da atividade de ectonucleotidases em plaquetas e agregação plaquetária em pacientes com lúpus eritematosos sistêmico |
spellingShingle |
Perfil da atividade de ectonucleotidases em plaquetas e agregação plaquetária em pacientes com lúpus eritematosos sistêmico Rosa, Cíntia Saydelles da LES Aterosclerose Plaquetas NTPDase E-NPP ADA Agregação plaquetária Systemic Lupus Erythematosus Atherosclerosis Platelets ENPP Platelets aggregation CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Perfil da atividade de ectonucleotidases em plaquetas e agregação plaquetária em pacientes com lúpus eritematosos sistêmico |
title_full |
Perfil da atividade de ectonucleotidases em plaquetas e agregação plaquetária em pacientes com lúpus eritematosos sistêmico |
title_fullStr |
Perfil da atividade de ectonucleotidases em plaquetas e agregação plaquetária em pacientes com lúpus eritematosos sistêmico |
title_full_unstemmed |
Perfil da atividade de ectonucleotidases em plaquetas e agregação plaquetária em pacientes com lúpus eritematosos sistêmico |
title_sort |
Perfil da atividade de ectonucleotidases em plaquetas e agregação plaquetária em pacientes com lúpus eritematosos sistêmico |
author |
Rosa, Cíntia Saydelles da |
author_facet |
Rosa, Cíntia Saydelles da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Morsch, Vera Maria Melchiors |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1519648219507868 |
dc.contributor.advisor-co1.fl_str_mv |
Schetinger, Maria Rosa Chitolina |
dc.contributor.referee1.fl_str_mv |
Mazzanti, Cinthia Melazzo Andrade |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/2886709251370905 |
dc.contributor.referee2.fl_str_mv |
Fachinetto, Roselei |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/7203076675431306 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8680932856088474 |
dc.contributor.author.fl_str_mv |
Rosa, Cíntia Saydelles da |
contributor_str_mv |
Morsch, Vera Maria Melchiors Schetinger, Maria Rosa Chitolina Mazzanti, Cinthia Melazzo Andrade Fachinetto, Roselei |
dc.subject.por.fl_str_mv |
LES Aterosclerose Plaquetas NTPDase E-NPP ADA Agregação plaquetária |
topic |
LES Aterosclerose Plaquetas NTPDase E-NPP ADA Agregação plaquetária Systemic Lupus Erythematosus Atherosclerosis Platelets ENPP Platelets aggregation CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Systemic Lupus Erythematosus Atherosclerosis Platelets ENPP Platelets aggregation |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Systemic Lupus Erythematosus (SLE) is a chronic inflammatory disease that affects mostly women in reproductive age and sometimes cause permanent damage. The development of cardiovascular disease, especially atherosclerosis, is the leading cause of death in SLE. However, its etiology and relationship with the development of atherosclerosis remain unknown. Several studies have shown that platelets have relevant properties to thrombogenesis, such as the release of ADP, a molecule capable of inducing platelet aggregation. Adenosine, derived from the hydrolysis of ATP and ADP, in turn has antiagreggant properties. The control of extracellular levels theses molecules and subsequent purinergic signaling induced by them is carried out by enzymes NTPDase, E-NPP, 5'-nucleotidase and ADA. Therefore, the objective of this study was to evaluate the activity of these ectonucleotidases in platelets and platelet aggregation profile in patients with LES. An increase in the activity of the enzymes NTPDase, E-NPP, 5'-nucleotidase and ADA was observed in platelets of patients with lupus compared with control subjects. No difference on platelet aggregation of patients with lupus was observed when compared to control. The increase in activity of E-NTPDase, E-NPP and 5'-nucleotidase seems to be a compensatory organic response against the pathological condition, to generate higher concentration of adenosine. But the ADA activity also is increased in platelets of patients with SLE and may decrease the concentration of adenosine, which favors prothrombotics process. Thus, the results suggest that the ectoenzymes may be involved in the modulation of atherosclerotic processes that occur in SLE. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-05-28 |
dc.date.accessioned.fl_str_mv |
2017-04-20 |
dc.date.available.fl_str_mv |
2017-04-20 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ROSA, Cíntia Saydelles da. Profilet of the ectonucleotidase activity and platelets agreggation in systemic erythematosus lupus patients. 2010. 74 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/11128 |
identifier_str_mv |
ROSA, Cíntia Saydelles da. Profilet of the ectonucleotidase activity and platelets agreggation in systemic erythematosus lupus patients. 2010. 74 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2010. |
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http://repositorio.ufsm.br/handle/1/11128 |
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