Avaliação química e atividade antioxidante das cascas e triterpenóides obtidos de Cariniana domestica (Mart) Miers
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/6040 |
Resumo: | The Lecythidaceae family consists of about 230 species, classified in some 10 genera. The species Cariniana domestica (Mart) Miers is one of several Jequitibá species, the oldest and millenary trees from Brazil. The present work developed studies with the aim of characterize the species when concerning to chemical aspects, in order to contribute to the knowledge improvement about Lecythidadeae family. The barks of C. domestica were collected in Tangará da Serra-MS. Exsiccate was archived as voucher specimen in the herbarium of Department of Biology at UFSM (SMDB 11818). Plant material was dried, crushed and powdered. The powder was submitted to hidroalcoolic maceration (7:3, v/v) and concentrated under reduced pressure. The remained aqueous extract was partitioned to obtain the fractions FCH2Cl2, F-AcOEt e F-BuOH. Chromatographic procedures led to the isolation of phytosterols (β-sitosterol + stigmasterol) and pentacyclic triterpenes (lupeol + β- amyrin), which were identified by means of spectroscopic (1H-NMR and 13C-NMR) and chromatographic (GC/MS and HPLC) methods. Additionally, the antioxidant capacity was evaluated by measuring its scavenging ability on DPPH and also using TBARS assay. Contents of total polyphenolic and flavonoid were measured as well as a chromatographic profile searching for phenolic substances was determined.. From F-CH2Cl2, DPPH assay revealed high scavenging ability with IC50 values that ranged from 6.5 ± 0.81 to 19.5 ± 1.32 μg/mL. IC50 values obtained with TBARS assay ranged from 11.11 ± 0.85 to 44.5 ± 2.31 μg/mL. Polyphenolic contents varied from 54.6 ± 0.33 to 309.3 ± 2.73 mg/g. Total flavonoid and condensed tannin contents varied from 12.0 ± 0.12 to 14.18 ± 0.1 and 131 ± 0.03 to 149.7 ± 0.22, respectively. HPLC analysis revealed the presence of gallic, caffeic and chlorogenic acids, as well as the flavonoids rutin, quercetin and kaempferol. The carotenoids lycopene and β- carotene were identified in dichloromethane fraction. The results revealed that the species C. domestica achieves antioxidant properties and the compounds identified for the first time for this species can be related to its popular uses. |
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Avaliação química e atividade antioxidante das cascas e triterpenóides obtidos de Cariniana domestica (Mart) MiersChemical evaluation and antioxidant activity of the barks and triterpenoids obtained from Cariniana domestica (Mart) MiersCariniana domesticaLecythidaceaeTriterpenosCLAEDPPHCariniana domesticaLecythidaceaeTriterpenesHPLCCNPQ::CIENCIAS DA SAUDE::FARMACIAThe Lecythidaceae family consists of about 230 species, classified in some 10 genera. The species Cariniana domestica (Mart) Miers is one of several Jequitibá species, the oldest and millenary trees from Brazil. The present work developed studies with the aim of characterize the species when concerning to chemical aspects, in order to contribute to the knowledge improvement about Lecythidadeae family. The barks of C. domestica were collected in Tangará da Serra-MS. Exsiccate was archived as voucher specimen in the herbarium of Department of Biology at UFSM (SMDB 11818). Plant material was dried, crushed and powdered. The powder was submitted to hidroalcoolic maceration (7:3, v/v) and concentrated under reduced pressure. The remained aqueous extract was partitioned to obtain the fractions FCH2Cl2, F-AcOEt e F-BuOH. Chromatographic procedures led to the isolation of phytosterols (β-sitosterol + stigmasterol) and pentacyclic triterpenes (lupeol + β- amyrin), which were identified by means of spectroscopic (1H-NMR and 13C-NMR) and chromatographic (GC/MS and HPLC) methods. Additionally, the antioxidant capacity was evaluated by measuring its scavenging ability on DPPH and also using TBARS assay. Contents of total polyphenolic and flavonoid were measured as well as a chromatographic profile searching for phenolic substances was determined.. From F-CH2Cl2, DPPH assay revealed high scavenging ability with IC50 values that ranged from 6.5 ± 0.81 to 19.5 ± 1.32 μg/mL. IC50 values obtained with TBARS assay ranged from 11.11 ± 0.85 to 44.5 ± 2.31 μg/mL. Polyphenolic contents varied from 54.6 ± 0.33 to 309.3 ± 2.73 mg/g. Total flavonoid and condensed tannin contents varied from 12.0 ± 0.12 to 14.18 ± 0.1 and 131 ± 0.03 to 149.7 ± 0.22, respectively. HPLC analysis revealed the presence of gallic, caffeic and chlorogenic acids, as well as the flavonoids rutin, quercetin and kaempferol. The carotenoids lycopene and β- carotene were identified in dichloromethane fraction. The results revealed that the species C. domestica achieves antioxidant properties and the compounds identified for the first time for this species can be related to its popular uses.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorA família Lecythidaceae consiste em cerca de 230 espécies, classificada em 10 gêneros. A espécie Cariniana domestica (Mart) Miers é uma das diversas espécies de Jequitibá, as árvores milenares mais antigas do Brasil. O presente estudo teve o objetivo de caracterizar quimicamente a espécie, visando contribuir para um maior conhecimento sobre a família Lecythidaceae. As cascas de C. domestica foram coletadas no município de Tangará da Serra-MS e o material foi catalogado sob o registro SMDB 11818 no departamento de Biologia da UFSM. O material vegetal foi seco, triturado e moído. O pó obtido foi submetido à maceração hidroalcóolica (7:3, v/v), seguindo-se de concentração em evaporador rotatório. A partição do extrato aquoso remanescente originou as frações F-CH2Cl2, F-AcOEt e F-BuOH. Os procedimentos cromatográficos de isolamento levaram à obtenção do fitoesteróis (β-sitosterol + estigmasterol) e triterpenos pentacíclicos (lupeol + β-amirina), os quais foram identificados por métodos espectroscópicos (1H-RMN e 13C-RMN) e cromatográficos (CG/EM e CLAE). Adicionalmente, foi avaliada a capacidade antioxidante frente ao radical DPPH, bem como utilizando o método do TBARS. Foram ainda realizadas dosagens investigativas quanto ao teor de polifenóis totais e flavonóides e um perfil cromatográfico para identificação de alguns compostos fenólicos também foi traçado. Os ensaios com o radical DPPH revelaram elevada capacidade seqüestradora de radicais livres, sendo que os valores de IC50 obtidos variaram de 6,5 ± 0,81 a 19,5 ± 1,32 μg/mL. No ensaio do TBARS, os valores de IC50 obtidos variaram de 11,11 ± 0,85 a 44,5 ± 2,31. A determinação de polifenóis totais revelou um teor variável de 54,6 ± 0,33 a 309,3 ± 2,73 mg/g. As determinações de flavonóides totais e taninos condensados variaram de 12,0 ± 0,12 a 14,18 ± 0,1 mg/g e 131 ± 0,03 a 149,7 ± 0,22, respectivamente. A análise por CLAE revelou a presença dos ácidos gálico, caféico e clorogênico, além dos flavonóides rutina, quercetina e campferol. Na fração diclorometano foram identificados os carotenóides licopeno e β-caroteno. Os resultados obtidos demonstram que a espécie C. domestica apresenta propriedades antioxidantes e os compostos identificados pela primeira vez para esta espécie podem estar relacionados com seus usos populares.Universidade Federal de Santa MariaBRFarmáciaUFSMPrograma de Pós-Graduação em Ciências FarmacêuticasAthayde, Margareth Lindehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707843T7Dalcol, Ionara Irionhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4797864E9Augusti, Paula Rossinihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4778049T7Janovik, Vanessa2017-03-022017-03-022011-03-31info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfJANOVIK, Vanessa. CHEMICAL EVALUATION AND ANTIOXIDANT ACTIVITY OF THE BARKS AND TRITERPENOIDS OBTAINED FROM CARINIANA DOMESTICA (MART) MIERS. 2011. 101 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2011.http://repositorio.ufsm.br/handle/1/6040porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-10-14T19:10:54Zoai:repositorio.ufsm.br:1/6040Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-10-14T19:10:54Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Avaliação química e atividade antioxidante das cascas e triterpenóides obtidos de Cariniana domestica (Mart) Miers Chemical evaluation and antioxidant activity of the barks and triterpenoids obtained from Cariniana domestica (Mart) Miers |
title |
Avaliação química e atividade antioxidante das cascas e triterpenóides obtidos de Cariniana domestica (Mart) Miers |
spellingShingle |
Avaliação química e atividade antioxidante das cascas e triterpenóides obtidos de Cariniana domestica (Mart) Miers Janovik, Vanessa Cariniana domestica Lecythidaceae Triterpenos CLAE DPPH Cariniana domestica Lecythidaceae Triterpenes HPLC CNPQ::CIENCIAS DA SAUDE::FARMACIA |
title_short |
Avaliação química e atividade antioxidante das cascas e triterpenóides obtidos de Cariniana domestica (Mart) Miers |
title_full |
Avaliação química e atividade antioxidante das cascas e triterpenóides obtidos de Cariniana domestica (Mart) Miers |
title_fullStr |
Avaliação química e atividade antioxidante das cascas e triterpenóides obtidos de Cariniana domestica (Mart) Miers |
title_full_unstemmed |
Avaliação química e atividade antioxidante das cascas e triterpenóides obtidos de Cariniana domestica (Mart) Miers |
title_sort |
Avaliação química e atividade antioxidante das cascas e triterpenóides obtidos de Cariniana domestica (Mart) Miers |
author |
Janovik, Vanessa |
author_facet |
Janovik, Vanessa |
author_role |
author |
dc.contributor.none.fl_str_mv |
Athayde, Margareth Linde http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707843T7 Dalcol, Ionara Irion http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4797864E9 Augusti, Paula Rossini http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4778049T7 |
dc.contributor.author.fl_str_mv |
Janovik, Vanessa |
dc.subject.por.fl_str_mv |
Cariniana domestica Lecythidaceae Triterpenos CLAE DPPH Cariniana domestica Lecythidaceae Triterpenes HPLC CNPQ::CIENCIAS DA SAUDE::FARMACIA |
topic |
Cariniana domestica Lecythidaceae Triterpenos CLAE DPPH Cariniana domestica Lecythidaceae Triterpenes HPLC CNPQ::CIENCIAS DA SAUDE::FARMACIA |
description |
The Lecythidaceae family consists of about 230 species, classified in some 10 genera. The species Cariniana domestica (Mart) Miers is one of several Jequitibá species, the oldest and millenary trees from Brazil. The present work developed studies with the aim of characterize the species when concerning to chemical aspects, in order to contribute to the knowledge improvement about Lecythidadeae family. The barks of C. domestica were collected in Tangará da Serra-MS. Exsiccate was archived as voucher specimen in the herbarium of Department of Biology at UFSM (SMDB 11818). Plant material was dried, crushed and powdered. The powder was submitted to hidroalcoolic maceration (7:3, v/v) and concentrated under reduced pressure. The remained aqueous extract was partitioned to obtain the fractions FCH2Cl2, F-AcOEt e F-BuOH. Chromatographic procedures led to the isolation of phytosterols (β-sitosterol + stigmasterol) and pentacyclic triterpenes (lupeol + β- amyrin), which were identified by means of spectroscopic (1H-NMR and 13C-NMR) and chromatographic (GC/MS and HPLC) methods. Additionally, the antioxidant capacity was evaluated by measuring its scavenging ability on DPPH and also using TBARS assay. Contents of total polyphenolic and flavonoid were measured as well as a chromatographic profile searching for phenolic substances was determined.. From F-CH2Cl2, DPPH assay revealed high scavenging ability with IC50 values that ranged from 6.5 ± 0.81 to 19.5 ± 1.32 μg/mL. IC50 values obtained with TBARS assay ranged from 11.11 ± 0.85 to 44.5 ± 2.31 μg/mL. Polyphenolic contents varied from 54.6 ± 0.33 to 309.3 ± 2.73 mg/g. Total flavonoid and condensed tannin contents varied from 12.0 ± 0.12 to 14.18 ± 0.1 and 131 ± 0.03 to 149.7 ± 0.22, respectively. HPLC analysis revealed the presence of gallic, caffeic and chlorogenic acids, as well as the flavonoids rutin, quercetin and kaempferol. The carotenoids lycopene and β- carotene were identified in dichloromethane fraction. The results revealed that the species C. domestica achieves antioxidant properties and the compounds identified for the first time for this species can be related to its popular uses. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-03-31 2017-03-02 2017-03-02 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
JANOVIK, Vanessa. CHEMICAL EVALUATION AND ANTIOXIDANT ACTIVITY OF THE BARKS AND TRITERPENOIDS OBTAINED FROM CARINIANA DOMESTICA (MART) MIERS. 2011. 101 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2011. http://repositorio.ufsm.br/handle/1/6040 |
identifier_str_mv |
JANOVIK, Vanessa. CHEMICAL EVALUATION AND ANTIOXIDANT ACTIVITY OF THE BARKS AND TRITERPENOIDS OBTAINED FROM CARINIANA DOMESTICA (MART) MIERS. 2011. 101 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2011. |
url |
http://repositorio.ufsm.br/handle/1/6040 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Farmácia UFSM Programa de Pós-Graduação em Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Farmácia UFSM Programa de Pós-Graduação em Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
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1805922112870809600 |