Efeito dos carotenóides licopeno e astaxantina sobre danos renais induzidos por cloreto de mercúrio
Autor(a) principal: | |
---|---|
Data de Publicação: | 2007 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/11167 |
Resumo: | Mercury is a heavy metal toxic for any living tissue, being kidneys the first target for the inorganic form. Oxidative stress has been pointed as an important molecular mechanism for kidney injury in inorganic mercury poisoning and the interaction of the metal with endogenous thiol-containing molecules, such as δ-aminolevulinate desidratase (δ-ALA-D), seems to contribute to this process. Lycopene and astaxanthin are plentiful carotenoids in tomatoes and algaes and seafoods, respectively. They have been widely studied because of their large antioxidant properties. This work evaluated the ability of lycopene and astaxanthin to prevent HgCl2 toxicity, assessing parameters like δ-ALA-D and glutathione S-transferase (GST) activities, non-protein sulfhydrylic groups content (NPSH), production of thiobarbituric acid reactive substances (TBARS) and activities of antioxidant enzymes like superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT), besides creatinine and uric acid plasma levels and histopathological analyses. Adult Wistar rats received lycopene or astaxanthin, by gavage, on doses of 0, 10, 25, or 50 mg/kg, six hours prior to the administration of 0 or 5 mg/kg HgCl2, yielding 8 experimental groups. After twelve hours of exposure to HgCl2 animals were killed. HgCl2 inhibited renal δ-ALA-D activity and increased TBARS levels in kidney and creatinine levels in plasma along with renal tubular necrosis. Lycopene prevented HgCl2-induced inhibition of δ-ALA-D activity and increase of lipid peroxidation in kidney, but not the increase in plasma creatinine levels or renal tubular necrosis caused by HgCl2. Although astaxanthin have not prevented HgCl2-induced inhibition of δ-ALA-D and increase in plasma creatinine levels, this carotenoid prevented lipid peroxidation and attenuated renal tubular necrosis caused by HgCl2. GPx and CAT activities were enhanced, while SOD activity was depressed, in mercury-treated rats when compared to control and these effects were prevented by some lycopene doses and by all astaxanthin doses evaluated. Some doses of lycopene negativelly affected antioxidant enzymes activities per se and the mechanism involved in this response has not been elucidated yet. Neither HgCl2 nor carotenoids treatment changed the content of NPSH groups or GST activity in kidney or uric acid levels in plasma. Our results indicate that although lycopene and astaxanthin did not prevent HgCl2-induced creatinine increase in plasma, changes in the activity of antioxidant enzymes might be limited by the use of these car |
id |
UFSM_311419fc0c012f5f6a033cef545abd02 |
---|---|
oai_identifier_str |
oai:repositorio.ufsm.br:1/11167 |
network_acronym_str |
UFSM |
network_name_str |
Biblioteca Digital de Teses e Dissertações do UFSM |
repository_id_str |
|
spelling |
2017-04-262017-04-262007-03-09AUGUSTI, Paula Rossini. Effect of lycopene and astaxanthin carotenoids on renal damage induced by mercuric chloride. 2007. 97 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2007.http://repositorio.ufsm.br/handle/1/11167Mercury is a heavy metal toxic for any living tissue, being kidneys the first target for the inorganic form. Oxidative stress has been pointed as an important molecular mechanism for kidney injury in inorganic mercury poisoning and the interaction of the metal with endogenous thiol-containing molecules, such as δ-aminolevulinate desidratase (δ-ALA-D), seems to contribute to this process. Lycopene and astaxanthin are plentiful carotenoids in tomatoes and algaes and seafoods, respectively. They have been widely studied because of their large antioxidant properties. This work evaluated the ability of lycopene and astaxanthin to prevent HgCl2 toxicity, assessing parameters like δ-ALA-D and glutathione S-transferase (GST) activities, non-protein sulfhydrylic groups content (NPSH), production of thiobarbituric acid reactive substances (TBARS) and activities of antioxidant enzymes like superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT), besides creatinine and uric acid plasma levels and histopathological analyses. Adult Wistar rats received lycopene or astaxanthin, by gavage, on doses of 0, 10, 25, or 50 mg/kg, six hours prior to the administration of 0 or 5 mg/kg HgCl2, yielding 8 experimental groups. After twelve hours of exposure to HgCl2 animals were killed. HgCl2 inhibited renal δ-ALA-D activity and increased TBARS levels in kidney and creatinine levels in plasma along with renal tubular necrosis. Lycopene prevented HgCl2-induced inhibition of δ-ALA-D activity and increase of lipid peroxidation in kidney, but not the increase in plasma creatinine levels or renal tubular necrosis caused by HgCl2. Although astaxanthin have not prevented HgCl2-induced inhibition of δ-ALA-D and increase in plasma creatinine levels, this carotenoid prevented lipid peroxidation and attenuated renal tubular necrosis caused by HgCl2. GPx and CAT activities were enhanced, while SOD activity was depressed, in mercury-treated rats when compared to control and these effects were prevented by some lycopene doses and by all astaxanthin doses evaluated. Some doses of lycopene negativelly affected antioxidant enzymes activities per se and the mechanism involved in this response has not been elucidated yet. Neither HgCl2 nor carotenoids treatment changed the content of NPSH groups or GST activity in kidney or uric acid levels in plasma. Our results indicate that although lycopene and astaxanthin did not prevent HgCl2-induced creatinine increase in plasma, changes in the activity of antioxidant enzymes might be limited by the use of these carO mercúrio é um metal pesado com toxicidade comprovada, capaz de causar danos em qualquer tecido com o qual tenha contato, sendo o rim o principal alvo para sua forma inorgânica. O estresse oxidativo tem sido apontado como um importante mecanismo molecular para a injúria renal induzida por mercúrio inorgânico e a interação desse metal com moléculas contendo grupos sulfidrílicos, tais como a enzima δ-aminolevulinato desidratase (δ-ALA-D), parece contribuir para esse processo. Licopeno e astaxantina são carotenóides abundantes em tomates e em algas e frutos do mar, respectivamente. Ambos têm sido amplamente estudados por suas propriedades antioxidantes. No presente estudo foi avaliado o efeito do licopeno e da astaxantina sobre a toxicidade do HgCl2 em rins de ratos, utilizando-se como parâmetros a atividade das enzimas δ-ALA-D e glutationa Stransferase (GST), quantidade de grupos sulfidrílicos não protéicos (NPSH), produção de substâncias reativas ao ácido tiobarbitúrico (TBARS), atividade das enzimas antioxidantes superóxido dismutase (SOD), glutationa peroxidase (GSH-Px) e catalase (CAT), além dos níveis plasmáticos de creatinina e ácido úrico e análise histopatológica. Ratos Wistar adultos receberam, por gavage, licopeno ou astaxantina nas doses de 0, 10, 25 ou 50 mg/kg, seis horas antes de receberem uma injeção subcutânea de HgCl2 (0 ou 5 mg/kg), resultando assim, em 8 grupos experimentais. Após doze horas da exposição ao HgCl2, os animais foram sacrificados. O HgCl2 inibiu a δ-ALA-D renal, aumentou a produção de TBARS e níveis plasmáticos de creatinina, além de causar necrose tubular. O licopeno preveniu a inibição da δ-ALA-D e a peroxidação lipídica, mas não preveniu o aumento de creatinina no plasma nem a ocorrência de necrose tubular induzidos pelo HgCl2. Embora a astaxantina não tenha prevenido a inibição da δ-ALA-D e o aumento nos níveis plasmáticos de creatinina, este carotenóide preveniu a ocorrência da peroxidação lipídica e atenuou a necrose tubular causada pelo HgCl2. As atividades das enzimas GSH-Px e CAT aumentaram, enquanto a atividade da SOD diminuiu nos animais tratados com HgCl2 e esses efeitos foram prevenidos por algumas doses de licopeno e por todas as doses de astaxantina avaliadas. Algumas doses de licopeno tiveram efeito negativo sobre as enzimas antioxidantes per se e o mecanismo envolvido nessa resposta ainda não foi elucidado. O tratamento com HgCl2 ou carotenóides não alterou o conteúdo de NPSH ou a atividade da GST no tecido renal, nem os níveis plasmáticos de ácido úrico. Os resultados indicam que embora licopeno e astaxantina não tenham prevenido o aumento nos níveis plasmáticos de creatinina induzido por HgCl2, as alterações nas enzimas antioxidantes podem ser limitadas pelo uso destes carotenóides.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBRBioquímicaδ-aminolevulinato desidrataseEnzimas antioxidantesNecrose tubularRatosSubstâncias reativas ao ácido tiobarbitúricoD-aminolevulinate dehydrataseAntioxidant enzymesTubular necrosisRatsThiobarbituric acid reactive substancesCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAEfeito dos carotenóides licopeno e astaxantina sobre danos renais induzidos por cloreto de mercúrioEffect of lycopene and astaxanthin carotenoids on renal damage induced by mercuric chlorideinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisEmanuelli, Tatianahttp://lattes.cnpq.br/2165391096880394Folmer, Vanderleihttp://lattes.cnpq.br/8135232309980269Costabeber, Ijoni Hildahttp://lattes.cnpq.br/2529905835093392http://lattes.cnpq.br/5389076387673136Augusti, Paula Rossini2008000000024005003005003007812e191-0e91-4b97-bc2b-b442502f018673b112be-28bb-4120-9a73-795bea29e75fb37e5cb4-b932-49c0-b276-cbcc78b961ed6ae00264-7600-4b63-9ac0-fce53c3cd6f9info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALPAULA AUGUSTI.pdfapplication/pdf573824http://repositorio.ufsm.br/bitstream/1/11167/1/PAULA%20AUGUSTI.pdf57c5334718c5cd287b02952e23bbe929MD51TEXTPAULA AUGUSTI.pdf.txtPAULA AUGUSTI.pdf.txtExtracted texttext/plain161457http://repositorio.ufsm.br/bitstream/1/11167/2/PAULA%20AUGUSTI.pdf.txtfb712b0fc6a1dbb1cff725609f6e50f2MD52THUMBNAILPAULA AUGUSTI.pdf.jpgPAULA AUGUSTI.pdf.jpgIM Thumbnailimage/jpeg6216http://repositorio.ufsm.br/bitstream/1/11167/3/PAULA%20AUGUSTI.pdf.jpg3512506781deadcba9a440e160e168e3MD531/111672022-01-31 14:25:55.004oai:repositorio.ufsm.br:1/11167Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-01-31T17:25:55Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Efeito dos carotenóides licopeno e astaxantina sobre danos renais induzidos por cloreto de mercúrio |
dc.title.alternative.eng.fl_str_mv |
Effect of lycopene and astaxanthin carotenoids on renal damage induced by mercuric chloride |
title |
Efeito dos carotenóides licopeno e astaxantina sobre danos renais induzidos por cloreto de mercúrio |
spellingShingle |
Efeito dos carotenóides licopeno e astaxantina sobre danos renais induzidos por cloreto de mercúrio Augusti, Paula Rossini δ-aminolevulinato desidratase Enzimas antioxidantes Necrose tubular Ratos Substâncias reativas ao ácido tiobarbitúrico D-aminolevulinate dehydratase Antioxidant enzymes Tubular necrosis Rats Thiobarbituric acid reactive substances CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Efeito dos carotenóides licopeno e astaxantina sobre danos renais induzidos por cloreto de mercúrio |
title_full |
Efeito dos carotenóides licopeno e astaxantina sobre danos renais induzidos por cloreto de mercúrio |
title_fullStr |
Efeito dos carotenóides licopeno e astaxantina sobre danos renais induzidos por cloreto de mercúrio |
title_full_unstemmed |
Efeito dos carotenóides licopeno e astaxantina sobre danos renais induzidos por cloreto de mercúrio |
title_sort |
Efeito dos carotenóides licopeno e astaxantina sobre danos renais induzidos por cloreto de mercúrio |
author |
Augusti, Paula Rossini |
author_facet |
Augusti, Paula Rossini |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Emanuelli, Tatiana |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2165391096880394 |
dc.contributor.referee1.fl_str_mv |
Folmer, Vanderlei |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/8135232309980269 |
dc.contributor.referee2.fl_str_mv |
Costabeber, Ijoni Hilda |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/2529905835093392 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5389076387673136 |
dc.contributor.author.fl_str_mv |
Augusti, Paula Rossini |
contributor_str_mv |
Emanuelli, Tatiana Folmer, Vanderlei Costabeber, Ijoni Hilda |
dc.subject.por.fl_str_mv |
δ-aminolevulinato desidratase Enzimas antioxidantes Necrose tubular Ratos Substâncias reativas ao ácido tiobarbitúrico |
topic |
δ-aminolevulinato desidratase Enzimas antioxidantes Necrose tubular Ratos Substâncias reativas ao ácido tiobarbitúrico D-aminolevulinate dehydratase Antioxidant enzymes Tubular necrosis Rats Thiobarbituric acid reactive substances CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
D-aminolevulinate dehydratase Antioxidant enzymes Tubular necrosis Rats Thiobarbituric acid reactive substances |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Mercury is a heavy metal toxic for any living tissue, being kidneys the first target for the inorganic form. Oxidative stress has been pointed as an important molecular mechanism for kidney injury in inorganic mercury poisoning and the interaction of the metal with endogenous thiol-containing molecules, such as δ-aminolevulinate desidratase (δ-ALA-D), seems to contribute to this process. Lycopene and astaxanthin are plentiful carotenoids in tomatoes and algaes and seafoods, respectively. They have been widely studied because of their large antioxidant properties. This work evaluated the ability of lycopene and astaxanthin to prevent HgCl2 toxicity, assessing parameters like δ-ALA-D and glutathione S-transferase (GST) activities, non-protein sulfhydrylic groups content (NPSH), production of thiobarbituric acid reactive substances (TBARS) and activities of antioxidant enzymes like superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT), besides creatinine and uric acid plasma levels and histopathological analyses. Adult Wistar rats received lycopene or astaxanthin, by gavage, on doses of 0, 10, 25, or 50 mg/kg, six hours prior to the administration of 0 or 5 mg/kg HgCl2, yielding 8 experimental groups. After twelve hours of exposure to HgCl2 animals were killed. HgCl2 inhibited renal δ-ALA-D activity and increased TBARS levels in kidney and creatinine levels in plasma along with renal tubular necrosis. Lycopene prevented HgCl2-induced inhibition of δ-ALA-D activity and increase of lipid peroxidation in kidney, but not the increase in plasma creatinine levels or renal tubular necrosis caused by HgCl2. Although astaxanthin have not prevented HgCl2-induced inhibition of δ-ALA-D and increase in plasma creatinine levels, this carotenoid prevented lipid peroxidation and attenuated renal tubular necrosis caused by HgCl2. GPx and CAT activities were enhanced, while SOD activity was depressed, in mercury-treated rats when compared to control and these effects were prevented by some lycopene doses and by all astaxanthin doses evaluated. Some doses of lycopene negativelly affected antioxidant enzymes activities per se and the mechanism involved in this response has not been elucidated yet. Neither HgCl2 nor carotenoids treatment changed the content of NPSH groups or GST activity in kidney or uric acid levels in plasma. Our results indicate that although lycopene and astaxanthin did not prevent HgCl2-induced creatinine increase in plasma, changes in the activity of antioxidant enzymes might be limited by the use of these car |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007-03-09 |
dc.date.accessioned.fl_str_mv |
2017-04-26 |
dc.date.available.fl_str_mv |
2017-04-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
AUGUSTI, Paula Rossini. Effect of lycopene and astaxanthin carotenoids on renal damage induced by mercuric chloride. 2007. 97 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2007. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/11167 |
identifier_str_mv |
AUGUSTI, Paula Rossini. Effect of lycopene and astaxanthin carotenoids on renal damage induced by mercuric chloride. 2007. 97 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2007. |
url |
http://repositorio.ufsm.br/handle/1/11167 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
200800000002 |
dc.relation.confidence.fl_str_mv |
400 500 300 500 300 |
dc.relation.authority.fl_str_mv |
7812e191-0e91-4b97-bc2b-b442502f0186 73b112be-28bb-4120-9a73-795bea29e75f b37e5cb4-b932-49c0-b276-cbcc78b961ed 6ae00264-7600-4b63-9ac0-fce53c3cd6f9 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Bioquímica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações do UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Biblioteca Digital de Teses e Dissertações do UFSM |
collection |
Biblioteca Digital de Teses e Dissertações do UFSM |
bitstream.url.fl_str_mv |
http://repositorio.ufsm.br/bitstream/1/11167/1/PAULA%20AUGUSTI.pdf http://repositorio.ufsm.br/bitstream/1/11167/2/PAULA%20AUGUSTI.pdf.txt http://repositorio.ufsm.br/bitstream/1/11167/3/PAULA%20AUGUSTI.pdf.jpg |
bitstream.checksum.fl_str_mv |
57c5334718c5cd287b02952e23bbe929 fb712b0fc6a1dbb1cff725609f6e50f2 3512506781deadcba9a440e160e168e3 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1791086283260428288 |