Características genotípicas e fenotípicas de cepas de Escherichia coli isoladas de carne moída bovina in natura
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/17370 |
Resumo: | Escherichia coli is a bacterium that comprise the normal intestinal flora of warm-blooded animals, responsible for a variety of diseases in humans, as bacteremia, urinary tract and intestinal infections. Some become pathogenic virulence genes after purchasing. It is a frequent contaminant of food, particularly those of animal origin, and when ingested, causes symptoms that characterize the foodborne Diseases such as diarrhea and malaise. The ground beef is a efficientdisseminator of E. coli because it has great nutritional and physicochemical qualities. In addition, a great handling contributes to crosscontamination. The objective of this research was to analyze genotypic and phenotypic characteristics of E. coli strains from ground beef in natura, marketed in a town in the Northwest region of Rio Grande do Sul from November 2012 to March 2013. For the Polymerase Chain Reaction (PCR), we made 26 pools of DNA samples of 262 E. coli strains previously isolated and identified. We investigated the presence of bfpA virulence genes (typical EPEC - tEPEC); eaeA (EPEC/EHEC); stx1 and stx2 (EHEC); hlyA (pO157 EHEC); aggR (EAEC); elt and est (ETEC); and ipaH (EIEC). The sensitivity profile front antimicrobial and the research phenotypes of resistance enzymes, ESBL, KPC, MBL and AmpC were performed by disk-diffusion method. Of the 26 pools analyzed 69.23% contained virulence genes. The bfpA gene was prevalent, present in 46.15% of the analyzed groups. The genes stx1, aggR, stx2, hlyA, eaeA and elt were found in 26.92%; 23.07%; 19.23%; 11.53%; 11.53%; and 3.84% respectively.In contrast they were not identified the genes est and ipaH. The sensitivity profile across antimicrobial showed 100% of sensitivity against aztreonam, ceftazidime, gentamicin, meropenem, and piperacillin/tazobactam and resistance against ampicillin (7.63%), sulfamethoxazole/trimethoprim (5.34%) and cephalothin (3.43%), and 1.14% being resistant to multiple drugs. Resistance enzymes were not detected by phenotypic test. Thus, better hygiene and sanitary precautions are necessary from time of animal slaughter to commercialization of ground beef and other meat products, in order to reduce contamination of feed, mainly by pathogenic microorganisms. Furthermore, greater awareness of the widespread use of antimicrobials in beef cattle breeding should be performed, avoiding the selection of resistant microorganisms to drugs commercially available for treating diseases |
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2019-07-09T19:39:25Z2019-07-09T19:39:25Z2016-01-15http://repositorio.ufsm.br/handle/1/17370Escherichia coli is a bacterium that comprise the normal intestinal flora of warm-blooded animals, responsible for a variety of diseases in humans, as bacteremia, urinary tract and intestinal infections. Some become pathogenic virulence genes after purchasing. It is a frequent contaminant of food, particularly those of animal origin, and when ingested, causes symptoms that characterize the foodborne Diseases such as diarrhea and malaise. The ground beef is a efficientdisseminator of E. coli because it has great nutritional and physicochemical qualities. In addition, a great handling contributes to crosscontamination. The objective of this research was to analyze genotypic and phenotypic characteristics of E. coli strains from ground beef in natura, marketed in a town in the Northwest region of Rio Grande do Sul from November 2012 to March 2013. For the Polymerase Chain Reaction (PCR), we made 26 pools of DNA samples of 262 E. coli strains previously isolated and identified. We investigated the presence of bfpA virulence genes (typical EPEC - tEPEC); eaeA (EPEC/EHEC); stx1 and stx2 (EHEC); hlyA (pO157 EHEC); aggR (EAEC); elt and est (ETEC); and ipaH (EIEC). The sensitivity profile front antimicrobial and the research phenotypes of resistance enzymes, ESBL, KPC, MBL and AmpC were performed by disk-diffusion method. Of the 26 pools analyzed 69.23% contained virulence genes. The bfpA gene was prevalent, present in 46.15% of the analyzed groups. The genes stx1, aggR, stx2, hlyA, eaeA and elt were found in 26.92%; 23.07%; 19.23%; 11.53%; 11.53%; and 3.84% respectively.In contrast they were not identified the genes est and ipaH. The sensitivity profile across antimicrobial showed 100% of sensitivity against aztreonam, ceftazidime, gentamicin, meropenem, and piperacillin/tazobactam and resistance against ampicillin (7.63%), sulfamethoxazole/trimethoprim (5.34%) and cephalothin (3.43%), and 1.14% being resistant to multiple drugs. Resistance enzymes were not detected by phenotypic test. Thus, better hygiene and sanitary precautions are necessary from time of animal slaughter to commercialization of ground beef and other meat products, in order to reduce contamination of feed, mainly by pathogenic microorganisms. Furthermore, greater awareness of the widespread use of antimicrobials in beef cattle breeding should be performed, avoiding the selection of resistant microorganisms to drugs commercially available for treating diseasesA Escherichia coli é uma bactéria que compõe a microbiota intestinal normal de animais de sangue quente, responsável por uma variedade de doenças em humanos, como bacteremia, infecção do trato urinário e infecções intestinais. Algumas tornam-se patogênicas após adquirirem genes de virulência. Ela é um contaminante frequente de alimentos, principalmente aqueles de origem animal e, quando ingerida, causa sintomas que caracterizam as Doenças Transmitidas por Alimentos, como diarreia e mal-estar. A carne moída é um eficiente veiculador de E. coli, pois possui ótimas qualidades nutricionais e físico-químicas. Além disso, a sua grande manipulação contribui para a contaminação cruzada. O objetivo desta pesquisa foi analisar características genotípicas e fenotípicas de cepas de E. coli, provenientes de carne moída in natura, comercializadas em um município da região Noroeste do Rio Grande do Sul, no período de novembro de 2012 a março de 2013. Para a Reação em Cadeia da Polimerase (PCR), foram realizados 26 pools de amostras de DNA de 262 cepas de E. coli, previamente isoladas e identificadas. Investigou-se a presença dos genes de virulência bfpA (EPEC típicas - tEPEC); eaeA (EPEC/EHEC); stx1 e stx2 (EHEC); hlyA (EHEC pO157); aggR (EAEC); elt e est (ETEC); e ipaH (EIEC). O perfil de sensibilidade frente aos antimicrobianos e a pesquisa dos fenótipos de enzimas de resistência ESBL, KPC, MBL e AmpC foram realizados pelo método de difusão do disco. Dos 26 pools analisados, 69,23% continham genes de virulência. O gene bfpA foi o prevalente, estando presente em 46,15% dos grupos analisados. Os genes stx1, aggR, stx2, hlyA, eaeA e elt foram encontrados em 26,92%; 23,07%; 19,23%; 11,53%; 11,53%; e 3,84%, respectivamente. Em contrapartida, não foram identificados os genes est e ipaH. O perfil de sensibilidade frente aos antimicrobianos demonstrou 100% de sensibilidade frente à aztreonam, ceftazidima, gentamicina, meropenem e piperacilina/tazobactam e resistência frente a ampicilina (7,63%), sulfametoxazol/trimetoprima (5,34%) e cefalotina (3,43%), sendo 1,14% resistente à múltiplas drogas. As enzimas de resistência não foram detectadas pelo teste fenotípico. Dessa forma, melhores cuidados higiênico-sanitários são necessários desde o momento do abate do animal até a comercialização da carne moída e outros produtos cárneos, a fim de diminuir a contaminação destes alimentos, principalmente por microrganismos patogênicos. Ainda, maior conscientização sobre o uso indiscriminado de antimicrobianos na criação de gado de corte deve ser realizada, evitando a seleção de microrganismos resistentes às drogas disponíveis comercialmente para tratamento de enfermidades.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências da SaúdePrograma de Pós-Graduação em Ciências FarmacêuticasUFSMBrasilAnálises Clínicas e ToxicológicasAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessEscherichia coliCarneVirulênciaAntimicrobianosMeatVirulenceAntimicrobialCNPQ::CIENCIAS DA SAUDE::FARMACIACaracterísticas genotípicas e fenotípicas de cepas de Escherichia coli isoladas de carne moída bovina in naturaCharacteristics genotypic and phenotypic of Escherichia coli strains isolated from ground beef meat in naturainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisHorner, Rosmarihttp://lattes.cnpq.br/5907084134183708Lavall, Marinês Calegarihttp://lattes.cnpq.br/9859155536607672Zanella, Janice de Fatima Pavanhttp://lattes.cnpq.br/2470330330007790http://lattes.cnpq.br/3472679922574025Damer, Juliana Raquel da Silva4003000000056005fdaffc5-abc5-4607-8952-fc5c4760b00479545c8e-3c9b-462b-b70e-bca670e30be2596c11be-3e2c-45fa-bb8c-9a47be748a07336b0780-5f69-4581-9719-af342346115breponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Características genotípicas e fenotípicas de cepas de Escherichia coli isoladas de carne moída bovina in natura |
dc.title.alternative.eng.fl_str_mv |
Characteristics genotypic and phenotypic of Escherichia coli strains isolated from ground beef meat in natura |
title |
Características genotípicas e fenotípicas de cepas de Escherichia coli isoladas de carne moída bovina in natura |
spellingShingle |
Características genotípicas e fenotípicas de cepas de Escherichia coli isoladas de carne moída bovina in natura Damer, Juliana Raquel da Silva Escherichia coli Carne Virulência Antimicrobianos Meat Virulence Antimicrobial CNPQ::CIENCIAS DA SAUDE::FARMACIA |
title_short |
Características genotípicas e fenotípicas de cepas de Escherichia coli isoladas de carne moída bovina in natura |
title_full |
Características genotípicas e fenotípicas de cepas de Escherichia coli isoladas de carne moída bovina in natura |
title_fullStr |
Características genotípicas e fenotípicas de cepas de Escherichia coli isoladas de carne moída bovina in natura |
title_full_unstemmed |
Características genotípicas e fenotípicas de cepas de Escherichia coli isoladas de carne moída bovina in natura |
title_sort |
Características genotípicas e fenotípicas de cepas de Escherichia coli isoladas de carne moída bovina in natura |
author |
Damer, Juliana Raquel da Silva |
author_facet |
Damer, Juliana Raquel da Silva |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Horner, Rosmari |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5907084134183708 |
dc.contributor.referee1.fl_str_mv |
Lavall, Marinês Calegari |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/9859155536607672 |
dc.contributor.referee2.fl_str_mv |
Zanella, Janice de Fatima Pavan |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/2470330330007790 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3472679922574025 |
dc.contributor.author.fl_str_mv |
Damer, Juliana Raquel da Silva |
contributor_str_mv |
Horner, Rosmari Lavall, Marinês Calegari Zanella, Janice de Fatima Pavan |
dc.subject.por.fl_str_mv |
Escherichia coli Carne Virulência Antimicrobianos |
topic |
Escherichia coli Carne Virulência Antimicrobianos Meat Virulence Antimicrobial CNPQ::CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
Meat Virulence Antimicrobial |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::FARMACIA |
description |
Escherichia coli is a bacterium that comprise the normal intestinal flora of warm-blooded animals, responsible for a variety of diseases in humans, as bacteremia, urinary tract and intestinal infections. Some become pathogenic virulence genes after purchasing. It is a frequent contaminant of food, particularly those of animal origin, and when ingested, causes symptoms that characterize the foodborne Diseases such as diarrhea and malaise. The ground beef is a efficientdisseminator of E. coli because it has great nutritional and physicochemical qualities. In addition, a great handling contributes to crosscontamination. The objective of this research was to analyze genotypic and phenotypic characteristics of E. coli strains from ground beef in natura, marketed in a town in the Northwest region of Rio Grande do Sul from November 2012 to March 2013. For the Polymerase Chain Reaction (PCR), we made 26 pools of DNA samples of 262 E. coli strains previously isolated and identified. We investigated the presence of bfpA virulence genes (typical EPEC - tEPEC); eaeA (EPEC/EHEC); stx1 and stx2 (EHEC); hlyA (pO157 EHEC); aggR (EAEC); elt and est (ETEC); and ipaH (EIEC). The sensitivity profile front antimicrobial and the research phenotypes of resistance enzymes, ESBL, KPC, MBL and AmpC were performed by disk-diffusion method. Of the 26 pools analyzed 69.23% contained virulence genes. The bfpA gene was prevalent, present in 46.15% of the analyzed groups. The genes stx1, aggR, stx2, hlyA, eaeA and elt were found in 26.92%; 23.07%; 19.23%; 11.53%; 11.53%; and 3.84% respectively.In contrast they were not identified the genes est and ipaH. The sensitivity profile across antimicrobial showed 100% of sensitivity against aztreonam, ceftazidime, gentamicin, meropenem, and piperacillin/tazobactam and resistance against ampicillin (7.63%), sulfamethoxazole/trimethoprim (5.34%) and cephalothin (3.43%), and 1.14% being resistant to multiple drugs. Resistance enzymes were not detected by phenotypic test. Thus, better hygiene and sanitary precautions are necessary from time of animal slaughter to commercialization of ground beef and other meat products, in order to reduce contamination of feed, mainly by pathogenic microorganisms. Furthermore, greater awareness of the widespread use of antimicrobials in beef cattle breeding should be performed, avoiding the selection of resistant microorganisms to drugs commercially available for treating diseases |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-01-15 |
dc.date.accessioned.fl_str_mv |
2019-07-09T19:39:25Z |
dc.date.available.fl_str_mv |
2019-07-09T19:39:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/17370 |
url |
http://repositorio.ufsm.br/handle/1/17370 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
400300000005 |
dc.relation.confidence.fl_str_mv |
600 |
dc.relation.authority.fl_str_mv |
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dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências da Saúde |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Farmacêuticas |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Análises Clínicas e Toxicológicas |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências da Saúde |
dc.source.none.fl_str_mv |
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instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
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collection |
Biblioteca Digital de Teses e Dissertações do UFSM |
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