Estudo fitoquímico e farmacológico de Jatropha isabellei Müll Arg.
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
dARK ID: | ark:/26339/001300000q8n5 |
Texto Completo: | http://repositorio.ufsm.br/handle/1/5963 |
Resumo: | Jatropha isabellei Müll Arg. belongs to the Euphorbiaceae family and is popularly known as yaguá-rova, turubiti and mamoneiro do campo. The decoction from the underground parts of this plant is popularly used to treat arthritis, gout, back pain and induce abortion. This work aims to determine the antioxidant capacity and antimicrobial activity of J. isabellei as well as to isolate the chemical constituents and to evaluate the popular use for gouty arthritis in an animal model. The underground parts of J. isabellei were collected in May 2008, in Cacequi, RS. The material is deposited in the herbarium of Department of Biology at Federal University of Santa Maria under register number SMDB 11.816. The plant material was dried, milled and macerated with the solvent ethanol:water (70:30, v/v). The fractions were obtained by fractionation of the crude extract with organic solvents of increase polarity (dichloromethane, ethyl acetate, n-butanol). The ethyl acetate fraction showed the best antioxidant activity against the DPPH free radical with IC50 de 14.78 ± 1.10 Sg/mL and inhibition profile similar to ascorbic acid. This fraction also showed the best values in the determination of phenolic compounds, flavonoids and condensed tannins. The crude extract and butanolic fractions showed antioxidant activity proportional to the dosage of phenolics. The dichloromethane fraction did not showed good antioxidant capacity, but allowed the isolation of a mixture of triterpenes in first time reported for this species, sitosterol 3-O-β-D glucoside and stigmasterol. The lipid peroxidation induced by Fe (II) showed that all the fractions at concentrations of 50 and 100 Sg/mL were able to inhibit significantly the production of TBARS, reaching the baseline levels when compared to control. In the antimicrobial activity the dichloromethane fraction showed the best results against the fungi S. schenckii e F. proliferatum and against bacteria of the Micrococcus genus. The crude extract of J. isabellei and its alkaloid purified fraction showed antinociceptive and antiedematogenic effects in an animal gouty arthritis model, scientifically proving the popular use of this plant. |
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Estudo fitoquímico e farmacológico de Jatropha isabellei Müll Arg.Phytochemical and pharmacological study of Jatropha isabellei Müll Arg.Jatropha isabelleiCapacidade antioxidanteTriterpenosAtividade antimicrobianaAtividade antinociceptiva e antiedematogênicaJatropha isabelleiAntioxidant capacityTriterpenesAntimicrobial activityAntinociceptive and antiedematogenic activitiesCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIAJatropha isabellei Müll Arg. belongs to the Euphorbiaceae family and is popularly known as yaguá-rova, turubiti and mamoneiro do campo. The decoction from the underground parts of this plant is popularly used to treat arthritis, gout, back pain and induce abortion. This work aims to determine the antioxidant capacity and antimicrobial activity of J. isabellei as well as to isolate the chemical constituents and to evaluate the popular use for gouty arthritis in an animal model. The underground parts of J. isabellei were collected in May 2008, in Cacequi, RS. The material is deposited in the herbarium of Department of Biology at Federal University of Santa Maria under register number SMDB 11.816. The plant material was dried, milled and macerated with the solvent ethanol:water (70:30, v/v). The fractions were obtained by fractionation of the crude extract with organic solvents of increase polarity (dichloromethane, ethyl acetate, n-butanol). The ethyl acetate fraction showed the best antioxidant activity against the DPPH free radical with IC50 de 14.78 ± 1.10 Sg/mL and inhibition profile similar to ascorbic acid. This fraction also showed the best values in the determination of phenolic compounds, flavonoids and condensed tannins. The crude extract and butanolic fractions showed antioxidant activity proportional to the dosage of phenolics. The dichloromethane fraction did not showed good antioxidant capacity, but allowed the isolation of a mixture of triterpenes in first time reported for this species, sitosterol 3-O-β-D glucoside and stigmasterol. The lipid peroxidation induced by Fe (II) showed that all the fractions at concentrations of 50 and 100 Sg/mL were able to inhibit significantly the production of TBARS, reaching the baseline levels when compared to control. In the antimicrobial activity the dichloromethane fraction showed the best results against the fungi S. schenckii e F. proliferatum and against bacteria of the Micrococcus genus. The crude extract of J. isabellei and its alkaloid purified fraction showed antinociceptive and antiedematogenic effects in an animal gouty arthritis model, scientifically proving the popular use of this plant.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorA espécie Jatropha isabellei Müll Arg pertence à família Euphorbiaceae e é conhecida popularmente como yaguá-rova, turubiti e mamoneiro do campo. A decocção das partes subterrâneas da planta é utilizada popularmente para tratar artrite, gota, dores nas costas e induzir o aborto. Este trabalho tem como objetivo determinar a capacidade antioxidante e a atividade antimicrobiana de J. isabellei, bem como, isolar consituintes químicos e avaliar o uso popular para atrite gotosa em modelo animal. As partes subterrâneas de J. isabellei foram coletadas em maio de 2008 em Cacequi, RS. O material testemunho está depositado no Herbário do Departamento de Biologia da UFSM catalogado sob número de registro 11.816. O material vegetal foi seco, moído e macerado com solvente etanol:água (70:30, v/v). As frações foram obtidas por fracionamento do extrato bruto com solventes orgânicos de polaridades crescentes (diclorometano, acetato de etila, n-butanol). A fração acetato de etila apresentou a melhor atividade antioxidante frente ao radical DPPH, com IC50 de 14,78 ± 1,10 Sg/mL e perfil de inibição semelhante ao ácido ascórbico. Esta fração também apresentou os melhores valores no doseamento de polifenóis, flavonóides e taninos condensados. O extrato bruto e a fração butanólica apresentaram atividade antioxidante proporcional ao doseamento dos ativos fenólicos. A fração diclorometano não apresentou boa capacidade antioxidante, mas proporcionou o isolamento de uma mistura de triterpenos relatados pela primeira vez na literatura para esta espécie, o sitosterol 3-O-β-D glicosídeo e estigmasterol. A indução da peroxidação lipídica por Fe(II) mostrou que todas as frações nas concentrações de 50 e 100 Sg/mL foram capazes de inibir significativamente a produção de TBARS alcançando os níveis basais, quando comparadas ao controle. Na atividade antimicrobiana, a fração diclorometano apresentou os melhores resultados frente aos fungos S. schenckii e F. proliferatum e frente a bactérias do gênero Micrococcus. O extrato bruto de J. isabellei e sua fração rica em alcalóides apresentaram efeitos antinociceptivos e antiedematogênicos em modelo animal de artrite gotosa, comprovando cientificamente o uso popular desta planta.Universidade Federal de Santa MariaBRFarmacologiaUFSMPrograma de Pós-Graduação em Ciências FarmacêuticasAthayde, Margareth Lindehttp://lattes.cnpq.br/7866111734540735Augusti, Paula Rossinihttp://lattes.cnpq.br/5389076387673136Dalcol, Ionara Irionhttp://lattes.cnpq.br/9769548819312192Fröhlich, Janaina Kieling2015-01-302015-01-302012-03-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfFRÖHLICH, Janaina Kieling. Phytochemical and pharmacological study of Jatropha isabellei Müll Arg.. 2012. 100 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2012.http://repositorio.ufsm.br/handle/1/5963ark:/26339/001300000q8n5porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2023-05-23T18:25:36Zoai:repositorio.ufsm.br:1/5963Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-05-23T18:25:36Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Estudo fitoquímico e farmacológico de Jatropha isabellei Müll Arg. Phytochemical and pharmacological study of Jatropha isabellei Müll Arg. |
title |
Estudo fitoquímico e farmacológico de Jatropha isabellei Müll Arg. |
spellingShingle |
Estudo fitoquímico e farmacológico de Jatropha isabellei Müll Arg. Fröhlich, Janaina Kieling Jatropha isabellei Capacidade antioxidante Triterpenos Atividade antimicrobiana Atividade antinociceptiva e antiedematogênica Jatropha isabellei Antioxidant capacity Triterpenes Antimicrobial activity Antinociceptive and antiedematogenic activities CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
title_short |
Estudo fitoquímico e farmacológico de Jatropha isabellei Müll Arg. |
title_full |
Estudo fitoquímico e farmacológico de Jatropha isabellei Müll Arg. |
title_fullStr |
Estudo fitoquímico e farmacológico de Jatropha isabellei Müll Arg. |
title_full_unstemmed |
Estudo fitoquímico e farmacológico de Jatropha isabellei Müll Arg. |
title_sort |
Estudo fitoquímico e farmacológico de Jatropha isabellei Müll Arg. |
author |
Fröhlich, Janaina Kieling |
author_facet |
Fröhlich, Janaina Kieling |
author_role |
author |
dc.contributor.none.fl_str_mv |
Athayde, Margareth Linde http://lattes.cnpq.br/7866111734540735 Augusti, Paula Rossini http://lattes.cnpq.br/5389076387673136 Dalcol, Ionara Irion http://lattes.cnpq.br/9769548819312192 |
dc.contributor.author.fl_str_mv |
Fröhlich, Janaina Kieling |
dc.subject.por.fl_str_mv |
Jatropha isabellei Capacidade antioxidante Triterpenos Atividade antimicrobiana Atividade antinociceptiva e antiedematogênica Jatropha isabellei Antioxidant capacity Triterpenes Antimicrobial activity Antinociceptive and antiedematogenic activities CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
topic |
Jatropha isabellei Capacidade antioxidante Triterpenos Atividade antimicrobiana Atividade antinociceptiva e antiedematogênica Jatropha isabellei Antioxidant capacity Triterpenes Antimicrobial activity Antinociceptive and antiedematogenic activities CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
description |
Jatropha isabellei Müll Arg. belongs to the Euphorbiaceae family and is popularly known as yaguá-rova, turubiti and mamoneiro do campo. The decoction from the underground parts of this plant is popularly used to treat arthritis, gout, back pain and induce abortion. This work aims to determine the antioxidant capacity and antimicrobial activity of J. isabellei as well as to isolate the chemical constituents and to evaluate the popular use for gouty arthritis in an animal model. The underground parts of J. isabellei were collected in May 2008, in Cacequi, RS. The material is deposited in the herbarium of Department of Biology at Federal University of Santa Maria under register number SMDB 11.816. The plant material was dried, milled and macerated with the solvent ethanol:water (70:30, v/v). The fractions were obtained by fractionation of the crude extract with organic solvents of increase polarity (dichloromethane, ethyl acetate, n-butanol). The ethyl acetate fraction showed the best antioxidant activity against the DPPH free radical with IC50 de 14.78 ± 1.10 Sg/mL and inhibition profile similar to ascorbic acid. This fraction also showed the best values in the determination of phenolic compounds, flavonoids and condensed tannins. The crude extract and butanolic fractions showed antioxidant activity proportional to the dosage of phenolics. The dichloromethane fraction did not showed good antioxidant capacity, but allowed the isolation of a mixture of triterpenes in first time reported for this species, sitosterol 3-O-β-D glucoside and stigmasterol. The lipid peroxidation induced by Fe (II) showed that all the fractions at concentrations of 50 and 100 Sg/mL were able to inhibit significantly the production of TBARS, reaching the baseline levels when compared to control. In the antimicrobial activity the dichloromethane fraction showed the best results against the fungi S. schenckii e F. proliferatum and against bacteria of the Micrococcus genus. The crude extract of J. isabellei and its alkaloid purified fraction showed antinociceptive and antiedematogenic effects in an animal gouty arthritis model, scientifically proving the popular use of this plant. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-03-26 2015-01-30 2015-01-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
FRÖHLICH, Janaina Kieling. Phytochemical and pharmacological study of Jatropha isabellei Müll Arg.. 2012. 100 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2012. http://repositorio.ufsm.br/handle/1/5963 |
dc.identifier.dark.fl_str_mv |
ark:/26339/001300000q8n5 |
identifier_str_mv |
FRÖHLICH, Janaina Kieling. Phytochemical and pharmacological study of Jatropha isabellei Müll Arg.. 2012. 100 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2012. ark:/26339/001300000q8n5 |
url |
http://repositorio.ufsm.br/handle/1/5963 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Farmacologia UFSM Programa de Pós-Graduação em Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Farmacologia UFSM Programa de Pós-Graduação em Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
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1815172378170228736 |