Estudo fitoquímico e farmacológico de Jatropha isabellei Müll Arg.

Detalhes bibliográficos
Autor(a) principal: Fröhlich, Janaina Kieling
Data de Publicação: 2012
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/001300000q8n5
Texto Completo: http://repositorio.ufsm.br/handle/1/5963
Resumo: Jatropha isabellei Müll Arg. belongs to the Euphorbiaceae family and is popularly known as yaguá-rova, turubiti and mamoneiro do campo. The decoction from the underground parts of this plant is popularly used to treat arthritis, gout, back pain and induce abortion. This work aims to determine the antioxidant capacity and antimicrobial activity of J. isabellei as well as to isolate the chemical constituents and to evaluate the popular use for gouty arthritis in an animal model. The underground parts of J. isabellei were collected in May 2008, in Cacequi, RS. The material is deposited in the herbarium of Department of Biology at Federal University of Santa Maria under register number SMDB 11.816. The plant material was dried, milled and macerated with the solvent ethanol:water (70:30, v/v). The fractions were obtained by fractionation of the crude extract with organic solvents of increase polarity (dichloromethane, ethyl acetate, n-butanol). The ethyl acetate fraction showed the best antioxidant activity against the DPPH free radical with IC50 de 14.78 ± 1.10 Sg/mL and inhibition profile similar to ascorbic acid. This fraction also showed the best values in the determination of phenolic compounds, flavonoids and condensed tannins. The crude extract and butanolic fractions showed antioxidant activity proportional to the dosage of phenolics. The dichloromethane fraction did not showed good antioxidant capacity, but allowed the isolation of a mixture of triterpenes in first time reported for this species, sitosterol 3-O-β-D glucoside and stigmasterol. The lipid peroxidation induced by Fe (II) showed that all the fractions at concentrations of 50 and 100 Sg/mL were able to inhibit significantly the production of TBARS, reaching the baseline levels when compared to control. In the antimicrobial activity the dichloromethane fraction showed the best results against the fungi S. schenckii e F. proliferatum and against bacteria of the Micrococcus genus. The crude extract of J. isabellei and its alkaloid purified fraction showed antinociceptive and antiedematogenic effects in an animal gouty arthritis model, scientifically proving the popular use of this plant.
id UFSM_399e29415db9eeef6e40f5423c3fcd70
oai_identifier_str oai:repositorio.ufsm.br:1/5963
network_acronym_str UFSM
network_name_str Manancial - Repositório Digital da UFSM
repository_id_str
spelling Estudo fitoquímico e farmacológico de Jatropha isabellei Müll Arg.Phytochemical and pharmacological study of Jatropha isabellei Müll Arg.Jatropha isabelleiCapacidade antioxidanteTriterpenosAtividade antimicrobianaAtividade antinociceptiva e antiedematogênicaJatropha isabelleiAntioxidant capacityTriterpenesAntimicrobial activityAntinociceptive and antiedematogenic activitiesCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIAJatropha isabellei Müll Arg. belongs to the Euphorbiaceae family and is popularly known as yaguá-rova, turubiti and mamoneiro do campo. The decoction from the underground parts of this plant is popularly used to treat arthritis, gout, back pain and induce abortion. This work aims to determine the antioxidant capacity and antimicrobial activity of J. isabellei as well as to isolate the chemical constituents and to evaluate the popular use for gouty arthritis in an animal model. The underground parts of J. isabellei were collected in May 2008, in Cacequi, RS. The material is deposited in the herbarium of Department of Biology at Federal University of Santa Maria under register number SMDB 11.816. The plant material was dried, milled and macerated with the solvent ethanol:water (70:30, v/v). The fractions were obtained by fractionation of the crude extract with organic solvents of increase polarity (dichloromethane, ethyl acetate, n-butanol). The ethyl acetate fraction showed the best antioxidant activity against the DPPH free radical with IC50 de 14.78 ± 1.10 Sg/mL and inhibition profile similar to ascorbic acid. This fraction also showed the best values in the determination of phenolic compounds, flavonoids and condensed tannins. The crude extract and butanolic fractions showed antioxidant activity proportional to the dosage of phenolics. The dichloromethane fraction did not showed good antioxidant capacity, but allowed the isolation of a mixture of triterpenes in first time reported for this species, sitosterol 3-O-β-D glucoside and stigmasterol. The lipid peroxidation induced by Fe (II) showed that all the fractions at concentrations of 50 and 100 Sg/mL were able to inhibit significantly the production of TBARS, reaching the baseline levels when compared to control. In the antimicrobial activity the dichloromethane fraction showed the best results against the fungi S. schenckii e F. proliferatum and against bacteria of the Micrococcus genus. The crude extract of J. isabellei and its alkaloid purified fraction showed antinociceptive and antiedematogenic effects in an animal gouty arthritis model, scientifically proving the popular use of this plant.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorA espécie Jatropha isabellei Müll Arg pertence à família Euphorbiaceae e é conhecida popularmente como yaguá-rova, turubiti e mamoneiro do campo. A decocção das partes subterrâneas da planta é utilizada popularmente para tratar artrite, gota, dores nas costas e induzir o aborto. Este trabalho tem como objetivo determinar a capacidade antioxidante e a atividade antimicrobiana de J. isabellei, bem como, isolar consituintes químicos e avaliar o uso popular para atrite gotosa em modelo animal. As partes subterrâneas de J. isabellei foram coletadas em maio de 2008 em Cacequi, RS. O material testemunho está depositado no Herbário do Departamento de Biologia da UFSM catalogado sob número de registro 11.816. O material vegetal foi seco, moído e macerado com solvente etanol:água (70:30, v/v). As frações foram obtidas por fracionamento do extrato bruto com solventes orgânicos de polaridades crescentes (diclorometano, acetato de etila, n-butanol). A fração acetato de etila apresentou a melhor atividade antioxidante frente ao radical DPPH, com IC50 de 14,78 ± 1,10 Sg/mL e perfil de inibição semelhante ao ácido ascórbico. Esta fração também apresentou os melhores valores no doseamento de polifenóis, flavonóides e taninos condensados. O extrato bruto e a fração butanólica apresentaram atividade antioxidante proporcional ao doseamento dos ativos fenólicos. A fração diclorometano não apresentou boa capacidade antioxidante, mas proporcionou o isolamento de uma mistura de triterpenos relatados pela primeira vez na literatura para esta espécie, o sitosterol 3-O-β-D glicosídeo e estigmasterol. A indução da peroxidação lipídica por Fe(II) mostrou que todas as frações nas concentrações de 50 e 100 Sg/mL foram capazes de inibir significativamente a produção de TBARS alcançando os níveis basais, quando comparadas ao controle. Na atividade antimicrobiana, a fração diclorometano apresentou os melhores resultados frente aos fungos S. schenckii e F. proliferatum e frente a bactérias do gênero Micrococcus. O extrato bruto de J. isabellei e sua fração rica em alcalóides apresentaram efeitos antinociceptivos e antiedematogênicos em modelo animal de artrite gotosa, comprovando cientificamente o uso popular desta planta.Universidade Federal de Santa MariaBRFarmacologiaUFSMPrograma de Pós-Graduação em Ciências FarmacêuticasAthayde, Margareth Lindehttp://lattes.cnpq.br/7866111734540735Augusti, Paula Rossinihttp://lattes.cnpq.br/5389076387673136Dalcol, Ionara Irionhttp://lattes.cnpq.br/9769548819312192Fröhlich, Janaina Kieling2015-01-302015-01-302012-03-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfFRÖHLICH, Janaina Kieling. Phytochemical and pharmacological study of Jatropha isabellei Müll Arg.. 2012. 100 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2012.http://repositorio.ufsm.br/handle/1/5963ark:/26339/001300000q8n5porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2023-05-23T18:25:36Zoai:repositorio.ufsm.br:1/5963Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-05-23T18:25:36Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Estudo fitoquímico e farmacológico de Jatropha isabellei Müll Arg.
Phytochemical and pharmacological study of Jatropha isabellei Müll Arg.
title Estudo fitoquímico e farmacológico de Jatropha isabellei Müll Arg.
spellingShingle Estudo fitoquímico e farmacológico de Jatropha isabellei Müll Arg.
Fröhlich, Janaina Kieling
Jatropha isabellei
Capacidade antioxidante
Triterpenos
Atividade antimicrobiana
Atividade antinociceptiva e antiedematogênica
Jatropha isabellei
Antioxidant capacity
Triterpenes
Antimicrobial activity
Antinociceptive and antiedematogenic activities
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
title_short Estudo fitoquímico e farmacológico de Jatropha isabellei Müll Arg.
title_full Estudo fitoquímico e farmacológico de Jatropha isabellei Müll Arg.
title_fullStr Estudo fitoquímico e farmacológico de Jatropha isabellei Müll Arg.
title_full_unstemmed Estudo fitoquímico e farmacológico de Jatropha isabellei Müll Arg.
title_sort Estudo fitoquímico e farmacológico de Jatropha isabellei Müll Arg.
author Fröhlich, Janaina Kieling
author_facet Fröhlich, Janaina Kieling
author_role author
dc.contributor.none.fl_str_mv Athayde, Margareth Linde
http://lattes.cnpq.br/7866111734540735
Augusti, Paula Rossini
http://lattes.cnpq.br/5389076387673136
Dalcol, Ionara Irion
http://lattes.cnpq.br/9769548819312192
dc.contributor.author.fl_str_mv Fröhlich, Janaina Kieling
dc.subject.por.fl_str_mv Jatropha isabellei
Capacidade antioxidante
Triterpenos
Atividade antimicrobiana
Atividade antinociceptiva e antiedematogênica
Jatropha isabellei
Antioxidant capacity
Triterpenes
Antimicrobial activity
Antinociceptive and antiedematogenic activities
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
topic Jatropha isabellei
Capacidade antioxidante
Triterpenos
Atividade antimicrobiana
Atividade antinociceptiva e antiedematogênica
Jatropha isabellei
Antioxidant capacity
Triterpenes
Antimicrobial activity
Antinociceptive and antiedematogenic activities
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
description Jatropha isabellei Müll Arg. belongs to the Euphorbiaceae family and is popularly known as yaguá-rova, turubiti and mamoneiro do campo. The decoction from the underground parts of this plant is popularly used to treat arthritis, gout, back pain and induce abortion. This work aims to determine the antioxidant capacity and antimicrobial activity of J. isabellei as well as to isolate the chemical constituents and to evaluate the popular use for gouty arthritis in an animal model. The underground parts of J. isabellei were collected in May 2008, in Cacequi, RS. The material is deposited in the herbarium of Department of Biology at Federal University of Santa Maria under register number SMDB 11.816. The plant material was dried, milled and macerated with the solvent ethanol:water (70:30, v/v). The fractions were obtained by fractionation of the crude extract with organic solvents of increase polarity (dichloromethane, ethyl acetate, n-butanol). The ethyl acetate fraction showed the best antioxidant activity against the DPPH free radical with IC50 de 14.78 ± 1.10 Sg/mL and inhibition profile similar to ascorbic acid. This fraction also showed the best values in the determination of phenolic compounds, flavonoids and condensed tannins. The crude extract and butanolic fractions showed antioxidant activity proportional to the dosage of phenolics. The dichloromethane fraction did not showed good antioxidant capacity, but allowed the isolation of a mixture of triterpenes in first time reported for this species, sitosterol 3-O-β-D glucoside and stigmasterol. The lipid peroxidation induced by Fe (II) showed that all the fractions at concentrations of 50 and 100 Sg/mL were able to inhibit significantly the production of TBARS, reaching the baseline levels when compared to control. In the antimicrobial activity the dichloromethane fraction showed the best results against the fungi S. schenckii e F. proliferatum and against bacteria of the Micrococcus genus. The crude extract of J. isabellei and its alkaloid purified fraction showed antinociceptive and antiedematogenic effects in an animal gouty arthritis model, scientifically proving the popular use of this plant.
publishDate 2012
dc.date.none.fl_str_mv 2012-03-26
2015-01-30
2015-01-30
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv FRÖHLICH, Janaina Kieling. Phytochemical and pharmacological study of Jatropha isabellei Müll Arg.. 2012. 100 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2012.
http://repositorio.ufsm.br/handle/1/5963
dc.identifier.dark.fl_str_mv ark:/26339/001300000q8n5
identifier_str_mv FRÖHLICH, Janaina Kieling. Phytochemical and pharmacological study of Jatropha isabellei Müll Arg.. 2012. 100 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2012.
ark:/26339/001300000q8n5
url http://repositorio.ufsm.br/handle/1/5963
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Farmacologia
UFSM
Programa de Pós-Graduação em Ciências Farmacêuticas
publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Farmacologia
UFSM
Programa de Pós-Graduação em Ciências Farmacêuticas
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
_version_ 1815172378170228736