Estudo longitudinal do perfil oxidativo em gestantes saudáveis e com complicações gestacionais
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/21712 |
Resumo: | During gestation, even though it is a physiological process, there is oxidative stress due to a greater metabolic demand and an increase in tissue oxygen consumption, especially in the placenta, in response to fetal growth and maternal physiological changes. Oxidative stress can be evaluated by the relationship between free radicals and antioxidants produced by the body. Excessive production of free radicals has detrimental effects, such as changes in tissue proteins, DNA damage, enzymes and carbohydrates, peroxidation of membrane lipids, among others. The sulfhydryl delta-aminolevulinate dehydratase enzyme (δ-ALA-D) may be inhibited in pro-oxidant situations and this inhibition results in the accumulation of 5-aminolevulinic acid, being directly related to an increase in the production of free radicals. Considering that oxidative stress is directly involved in the physiology of pregnancy, where mainly the placenta is susceptible to damage, thus favoring the development of gestational diseases such as gestational hypertension, preeclampsia and Gestational Diabetes Mellitus. The aim of this study was to evaluate the oxidative profile and activity of the δ-ALA-D enzyme throughout the three trimesters of pregnancy, besides to compare the oxidative profile between pregnant women with and without gestational complications. Oxidative damage parameters were analyzed by quantifying thiobarbituric acid reactive species (TBARS) and nitric oxide (NOx). The antioxidant system evaluation was performed by quantifying protein (P-SH) and non-protein (NP-SH) thiols groups, vitamin C levels, total antioxidant capacity (TAC), plasma iron reduction ability (FRAP), besides determining the activity of the enzymes catalase and δ-ALA-D in pregnant women. According to the results obtained in the present study, the oxidative profile seems to be altered mainly in the second gestational trimester, with an increase in oxidative damage markers and a decrease in antioxidants when compared to the first and third gestational trimester. In addition, oxidative damage markers were significantly higher in pregnant women with gestational complications, while their antioxidant system appears to be decreased due to reduced levels of P-SH, NP-SH, vitamin C, TAC and FRAP, besides decreased activity of catalase and δ-ALA-D enzymes. Because of this, we can conclude that there is a different oxidative response profile in each gestational trimester, as well as an increase in oxidative stress, a decrease in antioxidants and δ- ALA-D activity in pregnant women with complications compared to healthy pregnant women. This may be related to the aggravation of gestational diseases and the evaluation of δ-ALA-D activity may be useful in monitoring the damage during pregnancy because it is an indirect marker of oxidative stress. |
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Estudo longitudinal do perfil oxidativo em gestantes saudáveis e com complicações gestacionaisLongitudinal study of the oxidative profile in healthy pregnant women and with complicationsδ-ALA-DEstresse oxidativoGrávidasPré-eclâmpsiaDiabetes mellitus gestacionalOxidative stressPregnantGestational diabetes mellitusCNPQ::CIENCIAS DA SAUDE::FARMACIADuring gestation, even though it is a physiological process, there is oxidative stress due to a greater metabolic demand and an increase in tissue oxygen consumption, especially in the placenta, in response to fetal growth and maternal physiological changes. Oxidative stress can be evaluated by the relationship between free radicals and antioxidants produced by the body. Excessive production of free radicals has detrimental effects, such as changes in tissue proteins, DNA damage, enzymes and carbohydrates, peroxidation of membrane lipids, among others. The sulfhydryl delta-aminolevulinate dehydratase enzyme (δ-ALA-D) may be inhibited in pro-oxidant situations and this inhibition results in the accumulation of 5-aminolevulinic acid, being directly related to an increase in the production of free radicals. Considering that oxidative stress is directly involved in the physiology of pregnancy, where mainly the placenta is susceptible to damage, thus favoring the development of gestational diseases such as gestational hypertension, preeclampsia and Gestational Diabetes Mellitus. The aim of this study was to evaluate the oxidative profile and activity of the δ-ALA-D enzyme throughout the three trimesters of pregnancy, besides to compare the oxidative profile between pregnant women with and without gestational complications. Oxidative damage parameters were analyzed by quantifying thiobarbituric acid reactive species (TBARS) and nitric oxide (NOx). The antioxidant system evaluation was performed by quantifying protein (P-SH) and non-protein (NP-SH) thiols groups, vitamin C levels, total antioxidant capacity (TAC), plasma iron reduction ability (FRAP), besides determining the activity of the enzymes catalase and δ-ALA-D in pregnant women. According to the results obtained in the present study, the oxidative profile seems to be altered mainly in the second gestational trimester, with an increase in oxidative damage markers and a decrease in antioxidants when compared to the first and third gestational trimester. In addition, oxidative damage markers were significantly higher in pregnant women with gestational complications, while their antioxidant system appears to be decreased due to reduced levels of P-SH, NP-SH, vitamin C, TAC and FRAP, besides decreased activity of catalase and δ-ALA-D enzymes. Because of this, we can conclude that there is a different oxidative response profile in each gestational trimester, as well as an increase in oxidative stress, a decrease in antioxidants and δ- ALA-D activity in pregnant women with complications compared to healthy pregnant women. This may be related to the aggravation of gestational diseases and the evaluation of δ-ALA-D activity may be useful in monitoring the damage during pregnancy because it is an indirect marker of oxidative stress.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESDurante a gestação, mesmo sendo um processo fisiológico, há estresse oxidativo devido a uma maior demanda metabólica e ao aumento do consumo de oxigênio nos tecidos, principalmente na placenta, em resposta ao crescimento fetal e as mudanças fisiológicas maternas. O estresse oxidativo pode ser avaliado pela relação entre os radicais livres e antioxidantes produzidos pelo organismo. Uma produção excessiva de radicais livres tem efeitos prejudiciais, tais como a alteração nas proteínas dos tecidos, danos ao DNA, enzimas e carboidratos, peroxidação dos lipídios de membrana, entre outros. A enzima sulfidrílica delta-aminolevulinato-desidratase (δ- ALA-D) pode estar inibida em situações pró-oxidantes e essa inibição resulta no acúmulo do ácido 5-aminolevulínico, estando relacionado a um aumento na produção de radicais livres. Considerando que o estresse oxidativo está diretamente envolvido na fisiologia da gestação, onde principalmente a placenta está suscetível aos danos, favorecendo, assim, o desenvolvimento de doenças gestacionais, como a hipertensão gestacional, pré-eclâmpsia e Diabetes Mellitus Gestacional. O objetivo desse trabalho foi avaliar o perfil oxidativo e a atividade da enzima δ- ALA-D ao longo dos três trimestres de gestação, além de comparar o perfil oxidativo entre as gestantes com e sem complicações gestacionais. Foram analisados parâmetros de dano oxidativo através da quantificação das espécies reativas ao ácido tiobarbitúrico (TBARS) e dosagem do óxido nítrico (NOx). Já a avaliação do sistema antioxidante foi realizada com a quantificação dos grupos tióis protéicos (P-SH) e não protéicos (NP-SH), os níveis de vitamina C, a capacidade antioxidante total (TAC), a habilidade plasmática de redução de ferro (FRAP), além da determinação da atividade das enzimas catalase e δ-ALA-D em gestantes. Conforme os resultados obtidos no presente estudo, o perfil oxidativo parece estar alterado principalmente no segundo trimestre gestacional, com um aumento nos marcadores de dano oxidativo e uma diminuição dos antioxidantes quando comparados ao primeiro e terceiro trimestre gestacional. Além disso, os marcadores de dano oxidativo foram significativamente maiores em gestantes com complicações gestacionais, enquanto que o sistema antioxidante das mesmas parece estar diminuído devido à redução nos níveis de P-SH, NP-SH, vitamina C, TAC e FRAP, além da diminuição na atividade das enzimas catalase e δ-ALA-D. Devido a isso, podemos concluir que há um perfil de resposta oxidativa diferente em cada trimestre gestacional, além de um aumento no estado de estresse oxidativo, diminuição nos antioxidantes e na atividade da δ-ALA-D em gestantes com complicações comparadas a gestantes saudáveis. Sendo que isso pode estar relacionado ao agravamento das doenças gestacionais e a avaliação da atividade da δ-ALA-D pode ser útil no acompanhamento dos danos durante o período gestacional por ser um marcador indireto de estresse oxidativo.Universidade Federal de Santa MariaBrasilAnálises Clínicas e ToxicológicasUFSMPrograma de Pós-Graduação em Ciências FarmacêuticasCentro de Ciências da SaúdeBernasconi, Thissiane de Lima Gonçalveshttp://lattes.cnpq.br/1968750829053627Boeck, Carina RodriguesBochi, Guilherme VargasBauermann, Liliane de FreitasSchuch, Natielen JacquesLucca, Leidiane de2021-08-03T21:50:58Z2021-08-03T21:50:58Z2019-09-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/21712porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2021-08-04T06:03:13Zoai:repositorio.ufsm.br:1/21712Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2021-08-04T06:03:13Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Estudo longitudinal do perfil oxidativo em gestantes saudáveis e com complicações gestacionais Longitudinal study of the oxidative profile in healthy pregnant women and with complications |
title |
Estudo longitudinal do perfil oxidativo em gestantes saudáveis e com complicações gestacionais |
spellingShingle |
Estudo longitudinal do perfil oxidativo em gestantes saudáveis e com complicações gestacionais Lucca, Leidiane de δ-ALA-D Estresse oxidativo Grávidas Pré-eclâmpsia Diabetes mellitus gestacional Oxidative stress Pregnant Gestational diabetes mellitus CNPQ::CIENCIAS DA SAUDE::FARMACIA |
title_short |
Estudo longitudinal do perfil oxidativo em gestantes saudáveis e com complicações gestacionais |
title_full |
Estudo longitudinal do perfil oxidativo em gestantes saudáveis e com complicações gestacionais |
title_fullStr |
Estudo longitudinal do perfil oxidativo em gestantes saudáveis e com complicações gestacionais |
title_full_unstemmed |
Estudo longitudinal do perfil oxidativo em gestantes saudáveis e com complicações gestacionais |
title_sort |
Estudo longitudinal do perfil oxidativo em gestantes saudáveis e com complicações gestacionais |
author |
Lucca, Leidiane de |
author_facet |
Lucca, Leidiane de |
author_role |
author |
dc.contributor.none.fl_str_mv |
Bernasconi, Thissiane de Lima Gonçalves http://lattes.cnpq.br/1968750829053627 Boeck, Carina Rodrigues Bochi, Guilherme Vargas Bauermann, Liliane de Freitas Schuch, Natielen Jacques |
dc.contributor.author.fl_str_mv |
Lucca, Leidiane de |
dc.subject.por.fl_str_mv |
δ-ALA-D Estresse oxidativo Grávidas Pré-eclâmpsia Diabetes mellitus gestacional Oxidative stress Pregnant Gestational diabetes mellitus CNPQ::CIENCIAS DA SAUDE::FARMACIA |
topic |
δ-ALA-D Estresse oxidativo Grávidas Pré-eclâmpsia Diabetes mellitus gestacional Oxidative stress Pregnant Gestational diabetes mellitus CNPQ::CIENCIAS DA SAUDE::FARMACIA |
description |
During gestation, even though it is a physiological process, there is oxidative stress due to a greater metabolic demand and an increase in tissue oxygen consumption, especially in the placenta, in response to fetal growth and maternal physiological changes. Oxidative stress can be evaluated by the relationship between free radicals and antioxidants produced by the body. Excessive production of free radicals has detrimental effects, such as changes in tissue proteins, DNA damage, enzymes and carbohydrates, peroxidation of membrane lipids, among others. The sulfhydryl delta-aminolevulinate dehydratase enzyme (δ-ALA-D) may be inhibited in pro-oxidant situations and this inhibition results in the accumulation of 5-aminolevulinic acid, being directly related to an increase in the production of free radicals. Considering that oxidative stress is directly involved in the physiology of pregnancy, where mainly the placenta is susceptible to damage, thus favoring the development of gestational diseases such as gestational hypertension, preeclampsia and Gestational Diabetes Mellitus. The aim of this study was to evaluate the oxidative profile and activity of the δ-ALA-D enzyme throughout the three trimesters of pregnancy, besides to compare the oxidative profile between pregnant women with and without gestational complications. Oxidative damage parameters were analyzed by quantifying thiobarbituric acid reactive species (TBARS) and nitric oxide (NOx). The antioxidant system evaluation was performed by quantifying protein (P-SH) and non-protein (NP-SH) thiols groups, vitamin C levels, total antioxidant capacity (TAC), plasma iron reduction ability (FRAP), besides determining the activity of the enzymes catalase and δ-ALA-D in pregnant women. According to the results obtained in the present study, the oxidative profile seems to be altered mainly in the second gestational trimester, with an increase in oxidative damage markers and a decrease in antioxidants when compared to the first and third gestational trimester. In addition, oxidative damage markers were significantly higher in pregnant women with gestational complications, while their antioxidant system appears to be decreased due to reduced levels of P-SH, NP-SH, vitamin C, TAC and FRAP, besides decreased activity of catalase and δ-ALA-D enzymes. Because of this, we can conclude that there is a different oxidative response profile in each gestational trimester, as well as an increase in oxidative stress, a decrease in antioxidants and δ- ALA-D activity in pregnant women with complications compared to healthy pregnant women. This may be related to the aggravation of gestational diseases and the evaluation of δ-ALA-D activity may be useful in monitoring the damage during pregnancy because it is an indirect marker of oxidative stress. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09-27 2021-08-03T21:50:58Z 2021-08-03T21:50:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/21712 |
url |
http://repositorio.ufsm.br/handle/1/21712 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Análises Clínicas e Toxicológicas UFSM Programa de Pós-Graduação em Ciências Farmacêuticas Centro de Ciências da Saúde |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Análises Clínicas e Toxicológicas UFSM Programa de Pós-Graduação em Ciências Farmacêuticas Centro de Ciências da Saúde |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
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1805922093048528896 |