Aspectos biológicos da interação Fusarium spp. e Trichoderma spp. em solo compactado de aveia preta e soja sob plantio direto
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
dARK ID: | ark:/26339/001300000w154 |
Texto Completo: | http://repositorio.ufsm.br/handle/1/3215 |
Resumo: | Fusarium spp. is the causal agent of root rot in several crops. In no tillage system, compacted areas favor the incidence of these diseases. These fungi can also infect the grains and produce mycotoxins. Trichoderma spp. has shown promising results and can be used in the integrated management of diseases caused by soilborne pathogens. The aims of this study was to quantify and correlate populations of Fusarium spp., Trichoderma spp. and others (fungi and bacteria) with physical characteristics indicative of soil compaction in the crops of oat and soybean; identify morphological and molecularly isolates of Fusarium spp.; genetically characterize isolates of Trichoderma spp.; assess the efficiency of in vitro and in vivo control of Trichoderma spp. versus Fusarium spp. ; and quantify the production of deoxynivalenol (DON) and zearalenone (ZEA) by Fusarium spp. Soil samples were collected at Victor Graeff (RS) in an area previously mapped regarding to soil compaction. The samples were taken at three depths (0-5, 5-10, and 10-15 cm) and in six compaction levels established by the measures of resistance to penetration (Rp) (points with higher Rp: 3.4, 4.6, and 5.0 MPa, and lower Rp: 0.3, 1.3, and 2.2 MPa). The samples were evaluated for fungal and microbial population (serial dilutions) and soil physical characteristics. Fungi of the genus Fusarium and Trichoderma, when present in serial dilutions, were isolated for further molecular and morphological identification (based on TEF-1α and ITS regions, respectively). Tests were performed in vitro (direct confrontation) and in vivo (in oat and soybean) to evaluate the control efficiency of Trichoderma spp. versus Fusarium spp. The production of DON and ZEA was measured by Elisa and immunoaffinity columns, respectively. In oat grown after soybean the population and physical characteristics of the soil were showed higher correlation, with the largest populations of Fusarium spp. and Trichoderma spp. found in depths of 5-10 and 10-15 cm, respectively. 13 species of Fusarium were identified and the TEF-1α region was efficient for the distinction among them. T. koningiopsis, T. tomentosum and T. asperellum were identified, totaling five isolates and all of them showed good potential for controlling Fusarium spp. in soybean. In oat, stood out as root growth promoters, increasing the fresh weight of seedlings. In soybean isolates of F. oxysporum and F. proliferatum were pathogenic and caused damping off of seedlings. For oat, the isolates of F. graminearum did not provide the observation of such symptoms. F. graminearum and F. solani produced both DON and ZEA, while F. proliferatum and F. oxysporum produced ZEA. |
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Aspectos biológicos da interação Fusarium spp. e Trichoderma spp. em solo compactado de aveia preta e soja sob plantio diretoBiological aspects of interaction Fusarium spp. and Trichoderma spp. in soil compacted of oat and soybean under no tillageFungosCaracterísticas edáficasPolimorfismosPatogenicidadeMicotoxinasFungiSoil characteristicsPolymorphismsPathogenicityMycotoxinsCNPQ::CIENCIAS AGRARIAS::AGRONOMIAFusarium spp. is the causal agent of root rot in several crops. In no tillage system, compacted areas favor the incidence of these diseases. These fungi can also infect the grains and produce mycotoxins. Trichoderma spp. has shown promising results and can be used in the integrated management of diseases caused by soilborne pathogens. The aims of this study was to quantify and correlate populations of Fusarium spp., Trichoderma spp. and others (fungi and bacteria) with physical characteristics indicative of soil compaction in the crops of oat and soybean; identify morphological and molecularly isolates of Fusarium spp.; genetically characterize isolates of Trichoderma spp.; assess the efficiency of in vitro and in vivo control of Trichoderma spp. versus Fusarium spp. ; and quantify the production of deoxynivalenol (DON) and zearalenone (ZEA) by Fusarium spp. Soil samples were collected at Victor Graeff (RS) in an area previously mapped regarding to soil compaction. The samples were taken at three depths (0-5, 5-10, and 10-15 cm) and in six compaction levels established by the measures of resistance to penetration (Rp) (points with higher Rp: 3.4, 4.6, and 5.0 MPa, and lower Rp: 0.3, 1.3, and 2.2 MPa). The samples were evaluated for fungal and microbial population (serial dilutions) and soil physical characteristics. Fungi of the genus Fusarium and Trichoderma, when present in serial dilutions, were isolated for further molecular and morphological identification (based on TEF-1α and ITS regions, respectively). Tests were performed in vitro (direct confrontation) and in vivo (in oat and soybean) to evaluate the control efficiency of Trichoderma spp. versus Fusarium spp. The production of DON and ZEA was measured by Elisa and immunoaffinity columns, respectively. In oat grown after soybean the population and physical characteristics of the soil were showed higher correlation, with the largest populations of Fusarium spp. and Trichoderma spp. found in depths of 5-10 and 10-15 cm, respectively. 13 species of Fusarium were identified and the TEF-1α region was efficient for the distinction among them. T. koningiopsis, T. tomentosum and T. asperellum were identified, totaling five isolates and all of them showed good potential for controlling Fusarium spp. in soybean. In oat, stood out as root growth promoters, increasing the fresh weight of seedlings. In soybean isolates of F. oxysporum and F. proliferatum were pathogenic and caused damping off of seedlings. For oat, the isolates of F. graminearum did not provide the observation of such symptoms. F. graminearum and F. solani produced both DON and ZEA, while F. proliferatum and F. oxysporum produced ZEA.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorFusarium spp. é o agente causal de podridões radiculares em diversas culturas. No sistema plantio direto, áreas compactadas favorecem a incidência dessas doenças. Esses fungos podem também infectar os grãos e produzir micotoxinas. Trichoderma spp. vem apresentando resultados promissores e pode ser utilizado no manejo integrado de doenças provocadas por patógenos de solo. Os objetivos deste trabalho foram: quantificar e correlacionar populações de Fusarium spp., Trichoderma spp. e outros (fungos e bactérias) em um solo com características físicas indicativas de compactação, nos cultivos de aveia preta e soja; identificar morfológica e molecularmente os isolados de Fusarium spp.; caracterizar geneticamente isolados de Trichoderma spp.; testar a eficiência de controle in vitro e in vivo de Trichoderma spp. versus Fusarium spp.; e quantificar a produção de deoxinivalenol (DON) e zearalenona (ZEA) pelos isolados de Fusarium spp. Amostras de solo foram coletadas no município de Victor Graeff (RS) em uma área previamente mapeada quanto à compactação do solo. As coletas foram feitas em três profundidades (0-5; 5-10 e 10-15 cm) e em seis níveis de compactação estabelecidos pelas medidas de resistência à penetração (Rp) (pontos com maior Rp: 3,4; 4,6 e 5,0 MPa; e menor Rp: 0,3; 1,3 e 2,2 MPa). Nas amostras coletadas foram realizadas avaliações de população fúngica e microbiana (diluições seriais) e de características físicas do solo. Fungos dos gêneros Fusarium e Trichoderma, quando presentes nas diluições seriais, foram isolados para posterior identificação morfológica e molecular (baseada nas regiões TEF-1α e ITS, respectivamente). Foram realizados testes in vitro (confrontação direta) e in vivo (em aveia preta e soja) para avaliar a eficiência de controle de Trichoderma spp. versus Fusarium spp. A produção de DON e ZEA foi verificada pelo método Elisa e colunas de imunoafinidade, respectivamente. Em aveia preta cultivada após a soja, as características físicas e populacionais do solo se correlacionaram mais pronunciadamente, sendo que maiores populações de Fusarium spp. e Trichoderma spp. foram encontradas nas profundidades 5-10 e 10-15 cm, respectivamente. Identificaram-se 13 espécies de Fusarium e a região TEF-1α foi eficiente para sua distinção. T. koningiopsis, T. tomentosum e T. asperellum foram identificados, totalizando cinco isolados e todos eles apresentaram bom potencial de controle de Fusarium spp. em soja. Em aveia preta, destacaram-se como promotores de crescimento radicular, incrementando o peso fresco de plântulas. Em soja, isolados de F. oxysporum e F. proliferatum foram patogênicos e provocaram tombamento de plântulas. Para aveia, os isolados de F. graminearum não proporcionaram a observação desse sintoma. F. graminearum e F. solani produziram tanto DON quanto ZEA, enquanto que F. proliferatum e F. oxysporum produziram apenas ZEA.Universidade Federal de Santa MariaBRAgronomiaUFSMPrograma de Pós-Graduação em AgronomiaBlume, Elenahttp://lattes.cnpq.br/3299996746493028Ponte, Emerson Medeiros Delhttp://lattes.cnpq.br/9460309107496381Deuner, Carolina Cardosohttp://lattes.cnpq.br/3029556593136573Santurio, Janio Moraishttp://lattes.cnpq.br/6316012260769979Antoniolli, Zaida Inêshttp://lattes.cnpq.br/4692942549618168Milanesi, Paola Mendes2017-05-152017-05-152012-08-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfapplication/pdfMILANESI, Paola Mendes. Biological aspects of interaction Fusarium spp. and Trichoderma spp. in soil compacted of oat and soybean under no tillage. 2012. 133 f. Tese (Doutorado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2012.http://repositorio.ufsm.br/handle/1/3215ark:/26339/001300000w154porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-04-08T13:51:14Zoai:repositorio.ufsm.br:1/3215Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-04-08T13:51:14Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Aspectos biológicos da interação Fusarium spp. e Trichoderma spp. em solo compactado de aveia preta e soja sob plantio direto Biological aspects of interaction Fusarium spp. and Trichoderma spp. in soil compacted of oat and soybean under no tillage |
title |
Aspectos biológicos da interação Fusarium spp. e Trichoderma spp. em solo compactado de aveia preta e soja sob plantio direto |
spellingShingle |
Aspectos biológicos da interação Fusarium spp. e Trichoderma spp. em solo compactado de aveia preta e soja sob plantio direto Milanesi, Paola Mendes Fungos Características edáficas Polimorfismos Patogenicidade Micotoxinas Fungi Soil characteristics Polymorphisms Pathogenicity Mycotoxins CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Aspectos biológicos da interação Fusarium spp. e Trichoderma spp. em solo compactado de aveia preta e soja sob plantio direto |
title_full |
Aspectos biológicos da interação Fusarium spp. e Trichoderma spp. em solo compactado de aveia preta e soja sob plantio direto |
title_fullStr |
Aspectos biológicos da interação Fusarium spp. e Trichoderma spp. em solo compactado de aveia preta e soja sob plantio direto |
title_full_unstemmed |
Aspectos biológicos da interação Fusarium spp. e Trichoderma spp. em solo compactado de aveia preta e soja sob plantio direto |
title_sort |
Aspectos biológicos da interação Fusarium spp. e Trichoderma spp. em solo compactado de aveia preta e soja sob plantio direto |
author |
Milanesi, Paola Mendes |
author_facet |
Milanesi, Paola Mendes |
author_role |
author |
dc.contributor.none.fl_str_mv |
Blume, Elena http://lattes.cnpq.br/3299996746493028 Ponte, Emerson Medeiros Del http://lattes.cnpq.br/9460309107496381 Deuner, Carolina Cardoso http://lattes.cnpq.br/3029556593136573 Santurio, Janio Morais http://lattes.cnpq.br/6316012260769979 Antoniolli, Zaida Inês http://lattes.cnpq.br/4692942549618168 |
dc.contributor.author.fl_str_mv |
Milanesi, Paola Mendes |
dc.subject.por.fl_str_mv |
Fungos Características edáficas Polimorfismos Patogenicidade Micotoxinas Fungi Soil characteristics Polymorphisms Pathogenicity Mycotoxins CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
topic |
Fungos Características edáficas Polimorfismos Patogenicidade Micotoxinas Fungi Soil characteristics Polymorphisms Pathogenicity Mycotoxins CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
Fusarium spp. is the causal agent of root rot in several crops. In no tillage system, compacted areas favor the incidence of these diseases. These fungi can also infect the grains and produce mycotoxins. Trichoderma spp. has shown promising results and can be used in the integrated management of diseases caused by soilborne pathogens. The aims of this study was to quantify and correlate populations of Fusarium spp., Trichoderma spp. and others (fungi and bacteria) with physical characteristics indicative of soil compaction in the crops of oat and soybean; identify morphological and molecularly isolates of Fusarium spp.; genetically characterize isolates of Trichoderma spp.; assess the efficiency of in vitro and in vivo control of Trichoderma spp. versus Fusarium spp. ; and quantify the production of deoxynivalenol (DON) and zearalenone (ZEA) by Fusarium spp. Soil samples were collected at Victor Graeff (RS) in an area previously mapped regarding to soil compaction. The samples were taken at three depths (0-5, 5-10, and 10-15 cm) and in six compaction levels established by the measures of resistance to penetration (Rp) (points with higher Rp: 3.4, 4.6, and 5.0 MPa, and lower Rp: 0.3, 1.3, and 2.2 MPa). The samples were evaluated for fungal and microbial population (serial dilutions) and soil physical characteristics. Fungi of the genus Fusarium and Trichoderma, when present in serial dilutions, were isolated for further molecular and morphological identification (based on TEF-1α and ITS regions, respectively). Tests were performed in vitro (direct confrontation) and in vivo (in oat and soybean) to evaluate the control efficiency of Trichoderma spp. versus Fusarium spp. The production of DON and ZEA was measured by Elisa and immunoaffinity columns, respectively. In oat grown after soybean the population and physical characteristics of the soil were showed higher correlation, with the largest populations of Fusarium spp. and Trichoderma spp. found in depths of 5-10 and 10-15 cm, respectively. 13 species of Fusarium were identified and the TEF-1α region was efficient for the distinction among them. T. koningiopsis, T. tomentosum and T. asperellum were identified, totaling five isolates and all of them showed good potential for controlling Fusarium spp. in soybean. In oat, stood out as root growth promoters, increasing the fresh weight of seedlings. In soybean isolates of F. oxysporum and F. proliferatum were pathogenic and caused damping off of seedlings. For oat, the isolates of F. graminearum did not provide the observation of such symptoms. F. graminearum and F. solani produced both DON and ZEA, while F. proliferatum and F. oxysporum produced ZEA. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-08-21 2017-05-15 2017-05-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
MILANESI, Paola Mendes. Biological aspects of interaction Fusarium spp. and Trichoderma spp. in soil compacted of oat and soybean under no tillage. 2012. 133 f. Tese (Doutorado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2012. http://repositorio.ufsm.br/handle/1/3215 |
dc.identifier.dark.fl_str_mv |
ark:/26339/001300000w154 |
identifier_str_mv |
MILANESI, Paola Mendes. Biological aspects of interaction Fusarium spp. and Trichoderma spp. in soil compacted of oat and soybean under no tillage. 2012. 133 f. Tese (Doutorado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2012. ark:/26339/001300000w154 |
url |
http://repositorio.ufsm.br/handle/1/3215 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Agronomia UFSM Programa de Pós-Graduação em Agronomia |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Agronomia UFSM Programa de Pós-Graduação em Agronomia |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
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UFSM |
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UFSM |
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Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
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1815172404901576704 |