Aspectos biológicos da interação Fusarium spp. e Trichoderma spp. em solo compactado de aveia preta e soja sob plantio direto

Detalhes bibliográficos
Autor(a) principal: Milanesi, Paola Mendes
Data de Publicação: 2012
Tipo de documento: Tese
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/001300000w154
Texto Completo: http://repositorio.ufsm.br/handle/1/3215
Resumo: Fusarium spp. is the causal agent of root rot in several crops. In no tillage system, compacted areas favor the incidence of these diseases. These fungi can also infect the grains and produce mycotoxins. Trichoderma spp. has shown promising results and can be used in the integrated management of diseases caused by soilborne pathogens. The aims of this study was to quantify and correlate populations of Fusarium spp., Trichoderma spp. and others (fungi and bacteria) with physical characteristics indicative of soil compaction in the crops of oat and soybean; identify morphological and molecularly isolates of Fusarium spp.; genetically characterize isolates of Trichoderma spp.; assess the efficiency of in vitro and in vivo control of Trichoderma spp. versus Fusarium spp. ; and quantify the production of deoxynivalenol (DON) and zearalenone (ZEA) by Fusarium spp. Soil samples were collected at Victor Graeff (RS) in an area previously mapped regarding to soil compaction. The samples were taken at three depths (0-5, 5-10, and 10-15 cm) and in six compaction levels established by the measures of resistance to penetration (Rp) (points with higher Rp: 3.4, 4.6, and 5.0 MPa, and lower Rp: 0.3, 1.3, and 2.2 MPa). The samples were evaluated for fungal and microbial population (serial dilutions) and soil physical characteristics. Fungi of the genus Fusarium and Trichoderma, when present in serial dilutions, were isolated for further molecular and morphological identification (based on TEF-1α and ITS regions, respectively). Tests were performed in vitro (direct confrontation) and in vivo (in oat and soybean) to evaluate the control efficiency of Trichoderma spp. versus Fusarium spp. The production of DON and ZEA was measured by Elisa and immunoaffinity columns, respectively. In oat grown after soybean the population and physical characteristics of the soil were showed higher correlation, with the largest populations of Fusarium spp. and Trichoderma spp. found in depths of 5-10 and 10-15 cm, respectively. 13 species of Fusarium were identified and the TEF-1α region was efficient for the distinction among them. T. koningiopsis, T. tomentosum and T. asperellum were identified, totaling five isolates and all of them showed good potential for controlling Fusarium spp. in soybean. In oat, stood out as root growth promoters, increasing the fresh weight of seedlings. In soybean isolates of F. oxysporum and F. proliferatum were pathogenic and caused damping off of seedlings. For oat, the isolates of F. graminearum did not provide the observation of such symptoms. F. graminearum and F. solani produced both DON and ZEA, while F. proliferatum and F. oxysporum produced ZEA.
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spelling Aspectos biológicos da interação Fusarium spp. e Trichoderma spp. em solo compactado de aveia preta e soja sob plantio diretoBiological aspects of interaction Fusarium spp. and Trichoderma spp. in soil compacted of oat and soybean under no tillageFungosCaracterísticas edáficasPolimorfismosPatogenicidadeMicotoxinasFungiSoil characteristicsPolymorphismsPathogenicityMycotoxinsCNPQ::CIENCIAS AGRARIAS::AGRONOMIAFusarium spp. is the causal agent of root rot in several crops. In no tillage system, compacted areas favor the incidence of these diseases. These fungi can also infect the grains and produce mycotoxins. Trichoderma spp. has shown promising results and can be used in the integrated management of diseases caused by soilborne pathogens. The aims of this study was to quantify and correlate populations of Fusarium spp., Trichoderma spp. and others (fungi and bacteria) with physical characteristics indicative of soil compaction in the crops of oat and soybean; identify morphological and molecularly isolates of Fusarium spp.; genetically characterize isolates of Trichoderma spp.; assess the efficiency of in vitro and in vivo control of Trichoderma spp. versus Fusarium spp. ; and quantify the production of deoxynivalenol (DON) and zearalenone (ZEA) by Fusarium spp. Soil samples were collected at Victor Graeff (RS) in an area previously mapped regarding to soil compaction. The samples were taken at three depths (0-5, 5-10, and 10-15 cm) and in six compaction levels established by the measures of resistance to penetration (Rp) (points with higher Rp: 3.4, 4.6, and 5.0 MPa, and lower Rp: 0.3, 1.3, and 2.2 MPa). The samples were evaluated for fungal and microbial population (serial dilutions) and soil physical characteristics. Fungi of the genus Fusarium and Trichoderma, when present in serial dilutions, were isolated for further molecular and morphological identification (based on TEF-1α and ITS regions, respectively). Tests were performed in vitro (direct confrontation) and in vivo (in oat and soybean) to evaluate the control efficiency of Trichoderma spp. versus Fusarium spp. The production of DON and ZEA was measured by Elisa and immunoaffinity columns, respectively. In oat grown after soybean the population and physical characteristics of the soil were showed higher correlation, with the largest populations of Fusarium spp. and Trichoderma spp. found in depths of 5-10 and 10-15 cm, respectively. 13 species of Fusarium were identified and the TEF-1α region was efficient for the distinction among them. T. koningiopsis, T. tomentosum and T. asperellum were identified, totaling five isolates and all of them showed good potential for controlling Fusarium spp. in soybean. In oat, stood out as root growth promoters, increasing the fresh weight of seedlings. In soybean isolates of F. oxysporum and F. proliferatum were pathogenic and caused damping off of seedlings. For oat, the isolates of F. graminearum did not provide the observation of such symptoms. F. graminearum and F. solani produced both DON and ZEA, while F. proliferatum and F. oxysporum produced ZEA.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorFusarium spp. é o agente causal de podridões radiculares em diversas culturas. No sistema plantio direto, áreas compactadas favorecem a incidência dessas doenças. Esses fungos podem também infectar os grãos e produzir micotoxinas. Trichoderma spp. vem apresentando resultados promissores e pode ser utilizado no manejo integrado de doenças provocadas por patógenos de solo. Os objetivos deste trabalho foram: quantificar e correlacionar populações de Fusarium spp., Trichoderma spp. e outros (fungos e bactérias) em um solo com características físicas indicativas de compactação, nos cultivos de aveia preta e soja; identificar morfológica e molecularmente os isolados de Fusarium spp.; caracterizar geneticamente isolados de Trichoderma spp.; testar a eficiência de controle in vitro e in vivo de Trichoderma spp. versus Fusarium spp.; e quantificar a produção de deoxinivalenol (DON) e zearalenona (ZEA) pelos isolados de Fusarium spp. Amostras de solo foram coletadas no município de Victor Graeff (RS) em uma área previamente mapeada quanto à compactação do solo. As coletas foram feitas em três profundidades (0-5; 5-10 e 10-15 cm) e em seis níveis de compactação estabelecidos pelas medidas de resistência à penetração (Rp) (pontos com maior Rp: 3,4; 4,6 e 5,0 MPa; e menor Rp: 0,3; 1,3 e 2,2 MPa). Nas amostras coletadas foram realizadas avaliações de população fúngica e microbiana (diluições seriais) e de características físicas do solo. Fungos dos gêneros Fusarium e Trichoderma, quando presentes nas diluições seriais, foram isolados para posterior identificação morfológica e molecular (baseada nas regiões TEF-1α e ITS, respectivamente). Foram realizados testes in vitro (confrontação direta) e in vivo (em aveia preta e soja) para avaliar a eficiência de controle de Trichoderma spp. versus Fusarium spp. A produção de DON e ZEA foi verificada pelo método Elisa e colunas de imunoafinidade, respectivamente. Em aveia preta cultivada após a soja, as características físicas e populacionais do solo se correlacionaram mais pronunciadamente, sendo que maiores populações de Fusarium spp. e Trichoderma spp. foram encontradas nas profundidades 5-10 e 10-15 cm, respectivamente. Identificaram-se 13 espécies de Fusarium e a região TEF-1α foi eficiente para sua distinção. T. koningiopsis, T. tomentosum e T. asperellum foram identificados, totalizando cinco isolados e todos eles apresentaram bom potencial de controle de Fusarium spp. em soja. Em aveia preta, destacaram-se como promotores de crescimento radicular, incrementando o peso fresco de plântulas. Em soja, isolados de F. oxysporum e F. proliferatum foram patogênicos e provocaram tombamento de plântulas. Para aveia, os isolados de F. graminearum não proporcionaram a observação desse sintoma. F. graminearum e F. solani produziram tanto DON quanto ZEA, enquanto que F. proliferatum e F. oxysporum produziram apenas ZEA.Universidade Federal de Santa MariaBRAgronomiaUFSMPrograma de Pós-Graduação em AgronomiaBlume, Elenahttp://lattes.cnpq.br/3299996746493028Ponte, Emerson Medeiros Delhttp://lattes.cnpq.br/9460309107496381Deuner, Carolina Cardosohttp://lattes.cnpq.br/3029556593136573Santurio, Janio Moraishttp://lattes.cnpq.br/6316012260769979Antoniolli, Zaida Inêshttp://lattes.cnpq.br/4692942549618168Milanesi, Paola Mendes2017-05-152017-05-152012-08-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfapplication/pdfMILANESI, Paola Mendes. Biological aspects of interaction Fusarium spp. and Trichoderma spp. in soil compacted of oat and soybean under no tillage. 2012. 133 f. Tese (Doutorado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2012.http://repositorio.ufsm.br/handle/1/3215ark:/26339/001300000w154porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-04-08T13:51:14Zoai:repositorio.ufsm.br:1/3215Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-04-08T13:51:14Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Aspectos biológicos da interação Fusarium spp. e Trichoderma spp. em solo compactado de aveia preta e soja sob plantio direto
Biological aspects of interaction Fusarium spp. and Trichoderma spp. in soil compacted of oat and soybean under no tillage
title Aspectos biológicos da interação Fusarium spp. e Trichoderma spp. em solo compactado de aveia preta e soja sob plantio direto
spellingShingle Aspectos biológicos da interação Fusarium spp. e Trichoderma spp. em solo compactado de aveia preta e soja sob plantio direto
Milanesi, Paola Mendes
Fungos
Características edáficas
Polimorfismos
Patogenicidade
Micotoxinas
Fungi
Soil characteristics
Polymorphisms
Pathogenicity
Mycotoxins
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA
title_short Aspectos biológicos da interação Fusarium spp. e Trichoderma spp. em solo compactado de aveia preta e soja sob plantio direto
title_full Aspectos biológicos da interação Fusarium spp. e Trichoderma spp. em solo compactado de aveia preta e soja sob plantio direto
title_fullStr Aspectos biológicos da interação Fusarium spp. e Trichoderma spp. em solo compactado de aveia preta e soja sob plantio direto
title_full_unstemmed Aspectos biológicos da interação Fusarium spp. e Trichoderma spp. em solo compactado de aveia preta e soja sob plantio direto
title_sort Aspectos biológicos da interação Fusarium spp. e Trichoderma spp. em solo compactado de aveia preta e soja sob plantio direto
author Milanesi, Paola Mendes
author_facet Milanesi, Paola Mendes
author_role author
dc.contributor.none.fl_str_mv Blume, Elena
http://lattes.cnpq.br/3299996746493028
Ponte, Emerson Medeiros Del
http://lattes.cnpq.br/9460309107496381
Deuner, Carolina Cardoso
http://lattes.cnpq.br/3029556593136573
Santurio, Janio Morais
http://lattes.cnpq.br/6316012260769979
Antoniolli, Zaida Inês
http://lattes.cnpq.br/4692942549618168
dc.contributor.author.fl_str_mv Milanesi, Paola Mendes
dc.subject.por.fl_str_mv Fungos
Características edáficas
Polimorfismos
Patogenicidade
Micotoxinas
Fungi
Soil characteristics
Polymorphisms
Pathogenicity
Mycotoxins
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA
topic Fungos
Características edáficas
Polimorfismos
Patogenicidade
Micotoxinas
Fungi
Soil characteristics
Polymorphisms
Pathogenicity
Mycotoxins
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA
description Fusarium spp. is the causal agent of root rot in several crops. In no tillage system, compacted areas favor the incidence of these diseases. These fungi can also infect the grains and produce mycotoxins. Trichoderma spp. has shown promising results and can be used in the integrated management of diseases caused by soilborne pathogens. The aims of this study was to quantify and correlate populations of Fusarium spp., Trichoderma spp. and others (fungi and bacteria) with physical characteristics indicative of soil compaction in the crops of oat and soybean; identify morphological and molecularly isolates of Fusarium spp.; genetically characterize isolates of Trichoderma spp.; assess the efficiency of in vitro and in vivo control of Trichoderma spp. versus Fusarium spp. ; and quantify the production of deoxynivalenol (DON) and zearalenone (ZEA) by Fusarium spp. Soil samples were collected at Victor Graeff (RS) in an area previously mapped regarding to soil compaction. The samples were taken at three depths (0-5, 5-10, and 10-15 cm) and in six compaction levels established by the measures of resistance to penetration (Rp) (points with higher Rp: 3.4, 4.6, and 5.0 MPa, and lower Rp: 0.3, 1.3, and 2.2 MPa). The samples were evaluated for fungal and microbial population (serial dilutions) and soil physical characteristics. Fungi of the genus Fusarium and Trichoderma, when present in serial dilutions, were isolated for further molecular and morphological identification (based on TEF-1α and ITS regions, respectively). Tests were performed in vitro (direct confrontation) and in vivo (in oat and soybean) to evaluate the control efficiency of Trichoderma spp. versus Fusarium spp. The production of DON and ZEA was measured by Elisa and immunoaffinity columns, respectively. In oat grown after soybean the population and physical characteristics of the soil were showed higher correlation, with the largest populations of Fusarium spp. and Trichoderma spp. found in depths of 5-10 and 10-15 cm, respectively. 13 species of Fusarium were identified and the TEF-1α region was efficient for the distinction among them. T. koningiopsis, T. tomentosum and T. asperellum were identified, totaling five isolates and all of them showed good potential for controlling Fusarium spp. in soybean. In oat, stood out as root growth promoters, increasing the fresh weight of seedlings. In soybean isolates of F. oxysporum and F. proliferatum were pathogenic and caused damping off of seedlings. For oat, the isolates of F. graminearum did not provide the observation of such symptoms. F. graminearum and F. solani produced both DON and ZEA, while F. proliferatum and F. oxysporum produced ZEA.
publishDate 2012
dc.date.none.fl_str_mv 2012-08-21
2017-05-15
2017-05-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv MILANESI, Paola Mendes. Biological aspects of interaction Fusarium spp. and Trichoderma spp. in soil compacted of oat and soybean under no tillage. 2012. 133 f. Tese (Doutorado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2012.
http://repositorio.ufsm.br/handle/1/3215
dc.identifier.dark.fl_str_mv ark:/26339/001300000w154
identifier_str_mv MILANESI, Paola Mendes. Biological aspects of interaction Fusarium spp. and Trichoderma spp. in soil compacted of oat and soybean under no tillage. 2012. 133 f. Tese (Doutorado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2012.
ark:/26339/001300000w154
url http://repositorio.ufsm.br/handle/1/3215
dc.language.iso.fl_str_mv por
language por
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dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Agronomia
UFSM
Programa de Pós-Graduação em Agronomia
publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Agronomia
UFSM
Programa de Pós-Graduação em Agronomia
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
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instname_str Universidade Federal de Santa Maria (UFSM)
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reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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