Estudo da peroxidação lipídica in vivo e in vitro em ratos infectados experimentalmente com Trypanosoma evansi
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
dARK ID: | ark:/26339/0013000017dqj |
Texto Completo: | http://repositorio.ufsm.br/handle/1/10068 |
Resumo: | The present study aimed to assess the lipid peroxidation and the susceptibility of erythrocytes in vitro peroxidation as indicators of oxidative damage in erythrocytes and its role to the pathogenesis of anemia during the early acute phase of Trypanosoma evansi infection in rats. Fifty male Wistar rats were randomly allotted into seven groups: three trypanosome-infected groups (T2, T4 and T6; n = 10 animals per group) and four uninfected controls (C0, C2, C4 and C6; n = 5 animals per group). Animals from trypanosome-infected groups were intraperitoneally injected with 106 trypanosomes. Blood sample was collected by cardiac puncture before infection (day 0; Group C0) or on the 2nd (C2 and T2), 4th (C4 and T4) and 6th (C6 and T6) day pos-infection (dpi). The number that follows the group name is referring to the day of the cardiac blood collection. The samples were analyzed for red blood cell count (RBC), hemoglobin (Hb) concentration, packed cell volume (PCV), plasma malondialdehyde (MDA) and in vitro peroxidation of erythrocytes. The mean values of the hematological indices gradually decreased in infected compared with the control rats. MDA was significantly increased (P < 0.001) on 6th dpi in infected versus controls and was negatively correlated with PCV (P < 0.001; R² = 0.372). The erythrocytes in vitro peroxidation values were higher in group T4 and T6 than control rats (P < 0.01). There was positively correlation with erythrocytes peroxidation (P < 0.001; R² = 0.414) and MDA. The results of this study indicate that T. evansi infection in rats is associated with oxidative stress, indicated by lipid peroxidation, and oxidative damage in erythrocytes membrane, as demonstrated by in vitro peroxidation. This may be one of the causes of anemia in acute trypanosomosis. |
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Estudo da peroxidação lipídica in vivo e in vitro em ratos infectados experimentalmente com Trypanosoma evansiStudy of lipid peroxidation in vivo and in vitro in rats experimentally infected with Trypanosoma evansiT. evansiAnemiaPeroxidação lipídicaTBARSRatosAnemiaLipid peroxidationRatsCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAThe present study aimed to assess the lipid peroxidation and the susceptibility of erythrocytes in vitro peroxidation as indicators of oxidative damage in erythrocytes and its role to the pathogenesis of anemia during the early acute phase of Trypanosoma evansi infection in rats. Fifty male Wistar rats were randomly allotted into seven groups: three trypanosome-infected groups (T2, T4 and T6; n = 10 animals per group) and four uninfected controls (C0, C2, C4 and C6; n = 5 animals per group). Animals from trypanosome-infected groups were intraperitoneally injected with 106 trypanosomes. Blood sample was collected by cardiac puncture before infection (day 0; Group C0) or on the 2nd (C2 and T2), 4th (C4 and T4) and 6th (C6 and T6) day pos-infection (dpi). The number that follows the group name is referring to the day of the cardiac blood collection. The samples were analyzed for red blood cell count (RBC), hemoglobin (Hb) concentration, packed cell volume (PCV), plasma malondialdehyde (MDA) and in vitro peroxidation of erythrocytes. The mean values of the hematological indices gradually decreased in infected compared with the control rats. MDA was significantly increased (P < 0.001) on 6th dpi in infected versus controls and was negatively correlated with PCV (P < 0.001; R² = 0.372). The erythrocytes in vitro peroxidation values were higher in group T4 and T6 than control rats (P < 0.01). There was positively correlation with erythrocytes peroxidation (P < 0.001; R² = 0.414) and MDA. The results of this study indicate that T. evansi infection in rats is associated with oxidative stress, indicated by lipid peroxidation, and oxidative damage in erythrocytes membrane, as demonstrated by in vitro peroxidation. This may be one of the causes of anemia in acute trypanosomosis.Conselho Nacional de Desenvolvimento Científico e TecnológicoO presente estudo teve como objetivo avaliar a peroxidação lipídica e a susceptibilidade de eritrócitos a peroxidação in vitro como indicadores de danos oxidativos em eritrócitos. Ainda, avaliar a sua participação no desenvolvimento da anemia na fase aguda da infecção por Trypanosoma evansi em ratos. Cinqüenta ratos Wistar, machos, foram distribuídos aleatoriamente em sete grupos, sendo: três grupos infectados com tripanossomas - T2, T4 e T6 (n = 10 animais por grupo) e quatro controles não infectados - C0, C2, C4 e C6 (n = 5 animais por grupo). Os animais foram infectados intraperitonealmente com sangue contendo106 tripanossomas. O número que compõe o nome do grupo indica o dia em que foi realizada a coleta sanguínea e subseqüente eutanásia, assim, o sangue foi coletado por punção cardíaca antes da infecção (dia 0; Grupo C0) e no 2° (C2 e T2), 4° (C4 e T4) e 6° (C6 e T6) dias após a infecção (dpi). Foram realizadas contagens de eritrócitos, concentração hemoglobina (Hb), hematócrito (Ht), nível de malondialdeído plasmático (MDA) e peroxidação de eritrócitos in vitro. Os valores médios dos índices hematológicos reduziram gradativamente nos animais infectados em comparação com o grupo controle. A média de MDA plasmático aumentou significativamente (P < 0,001) no 6° dpi nos animais infectados comparado com o grupo controle e, foi negativamente correlacionada com o Ht (P < 0,001, R² = 0,372). Os valores da peroxidação dos eritrócitos in vitro foram maiores no grupo T4 e T6 que nos grupos controles (P < 0,01). Ainda, houve correlação positiva com peroxidação eritrocitária in vitro e MDA (P < 0,001, R² = 0,414). Os resultados obtidos indicaram que a infecção por T. evansi em ratos está associada com o estresse oxidativo, indicada pelo aumento da peroxidação lipídica, e a danos oxidativos na membrana dos eritrócitos, como demonstrado pelo peroxidação in vitro. Diante disso, danos oxidativos nos eritrócitos podem ser uma possível causa do desenvolvimento de anemia na infecção aguda por T. evansi em ratos.Universidade Federal de Santa MariaBRMedicina VeterináriaUFSMPrograma de Pós-Graduação em Medicina VeterináriaLopes, Sonia Terezinha dos Anjoshttp://lattes.cnpq.br/8059723754130756Monteiro, Silvia Gonzalezhttp://lattes.cnpq.br/3762606653182779Mazzanti, Cinthia Melazzo Andradehttp://lattes.cnpq.br/2886709251370905Wolkmer, Patricia2010-04-292010-04-292009-03-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfWOLKMER, Patricia. Study of lipid peroxidation in vivo and in vitro in rats experimentally infected with Trypanosoma evansi. 2009. 48 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2009.http://repositorio.ufsm.br/handle/1/10068ark:/26339/0013000017dqjporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-01-26T18:20:29Zoai:repositorio.ufsm.br:1/10068Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-01-26T18:20:29Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Estudo da peroxidação lipídica in vivo e in vitro em ratos infectados experimentalmente com Trypanosoma evansi Study of lipid peroxidation in vivo and in vitro in rats experimentally infected with Trypanosoma evansi |
title |
Estudo da peroxidação lipídica in vivo e in vitro em ratos infectados experimentalmente com Trypanosoma evansi |
spellingShingle |
Estudo da peroxidação lipídica in vivo e in vitro em ratos infectados experimentalmente com Trypanosoma evansi Wolkmer, Patricia T. evansi Anemia Peroxidação lipídica TBARS Ratos Anemia Lipid peroxidation Rats CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Estudo da peroxidação lipídica in vivo e in vitro em ratos infectados experimentalmente com Trypanosoma evansi |
title_full |
Estudo da peroxidação lipídica in vivo e in vitro em ratos infectados experimentalmente com Trypanosoma evansi |
title_fullStr |
Estudo da peroxidação lipídica in vivo e in vitro em ratos infectados experimentalmente com Trypanosoma evansi |
title_full_unstemmed |
Estudo da peroxidação lipídica in vivo e in vitro em ratos infectados experimentalmente com Trypanosoma evansi |
title_sort |
Estudo da peroxidação lipídica in vivo e in vitro em ratos infectados experimentalmente com Trypanosoma evansi |
author |
Wolkmer, Patricia |
author_facet |
Wolkmer, Patricia |
author_role |
author |
dc.contributor.none.fl_str_mv |
Lopes, Sonia Terezinha dos Anjos http://lattes.cnpq.br/8059723754130756 Monteiro, Silvia Gonzalez http://lattes.cnpq.br/3762606653182779 Mazzanti, Cinthia Melazzo Andrade http://lattes.cnpq.br/2886709251370905 |
dc.contributor.author.fl_str_mv |
Wolkmer, Patricia |
dc.subject.por.fl_str_mv |
T. evansi Anemia Peroxidação lipídica TBARS Ratos Anemia Lipid peroxidation Rats CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
topic |
T. evansi Anemia Peroxidação lipídica TBARS Ratos Anemia Lipid peroxidation Rats CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The present study aimed to assess the lipid peroxidation and the susceptibility of erythrocytes in vitro peroxidation as indicators of oxidative damage in erythrocytes and its role to the pathogenesis of anemia during the early acute phase of Trypanosoma evansi infection in rats. Fifty male Wistar rats were randomly allotted into seven groups: three trypanosome-infected groups (T2, T4 and T6; n = 10 animals per group) and four uninfected controls (C0, C2, C4 and C6; n = 5 animals per group). Animals from trypanosome-infected groups were intraperitoneally injected with 106 trypanosomes. Blood sample was collected by cardiac puncture before infection (day 0; Group C0) or on the 2nd (C2 and T2), 4th (C4 and T4) and 6th (C6 and T6) day pos-infection (dpi). The number that follows the group name is referring to the day of the cardiac blood collection. The samples were analyzed for red blood cell count (RBC), hemoglobin (Hb) concentration, packed cell volume (PCV), plasma malondialdehyde (MDA) and in vitro peroxidation of erythrocytes. The mean values of the hematological indices gradually decreased in infected compared with the control rats. MDA was significantly increased (P < 0.001) on 6th dpi in infected versus controls and was negatively correlated with PCV (P < 0.001; R² = 0.372). The erythrocytes in vitro peroxidation values were higher in group T4 and T6 than control rats (P < 0.01). There was positively correlation with erythrocytes peroxidation (P < 0.001; R² = 0.414) and MDA. The results of this study indicate that T. evansi infection in rats is associated with oxidative stress, indicated by lipid peroxidation, and oxidative damage in erythrocytes membrane, as demonstrated by in vitro peroxidation. This may be one of the causes of anemia in acute trypanosomosis. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-03-02 2010-04-29 2010-04-29 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
WOLKMER, Patricia. Study of lipid peroxidation in vivo and in vitro in rats experimentally infected with Trypanosoma evansi. 2009. 48 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2009. http://repositorio.ufsm.br/handle/1/10068 |
dc.identifier.dark.fl_str_mv |
ark:/26339/0013000017dqj |
identifier_str_mv |
WOLKMER, Patricia. Study of lipid peroxidation in vivo and in vitro in rats experimentally infected with Trypanosoma evansi. 2009. 48 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2009. ark:/26339/0013000017dqj |
url |
http://repositorio.ufsm.br/handle/1/10068 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Medicina Veterinária UFSM Programa de Pós-Graduação em Medicina Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Medicina Veterinária UFSM Programa de Pós-Graduação em Medicina Veterinária |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1815172466028314624 |