Produção de celulases por Gelatoporia subvermispora para hidrólise de material celulósico
Main Author: | |
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Publication Date: | 2014 |
Format: | Master thesis |
Language: | por |
Source: | Biblioteca Digital de Teses e Dissertações do UFSM |
Download full: | http://repositorio.ufsm.br/handle/1/5586 |
Summary: | Several microorganisms are able of producing enzymes responsible for the conversion of biomass to ethanol, such as cellulases. However, studies about diversity and biotechnological application of microorganisms from Pampa biome are still scarce. To try to reduce this knowledge lack, the Gelatoporia subvermispora fungus was isolated from the Pampa biome, resulting in the first occurrence of this specie in Latin America. Commercially, cellulases are produced by submerged fermentation. However, filamentous fungi that are considered good producers of cellulases show better results in solid-state fermentation, mainly by the similarity between the solid medium and the natural habitat of these microorganisms. In order to evaluate the biotechnological potential of the fungi G. subvermispora for cellulases production, rice straw, sewage sludge and sugar cane bagasse were used as substrate. The highest values for the total cellulolytic activity by the method of filter paper (FP) were obtained using sugarcane bagasse as substrate, reaching the value of 3.82 FPU.g-1. The sewage sludge was an excellent medium for the production of xylanase and exo-cellulases, reaching peak activity of 227.97 U.g-1 and 134.25 U.g-1, respectively. The endo-cellulase activity was similar in almost whole substrates tested, as showed in the runs 11 for rice straw (40.75 U.g-1) and 14 for sewage sludge (35.32 U.g-1). Subsequently, cellulolytic enzymes from fungal Gelatoporia subvermispora produced by solid-state fermentation using different substrates were applied to hydrolyze non-treated sugarcane bagasse using indirect sonication. The best results were obtained using sewage sludge as substrate for enzyme production. The mean yields obtained with enzymes produced using sewage sludge, sugarcane bagasse and rice straw as substrates were 72.8, 58.7 and 51.2 g.kg-1, respectively. Regarding the use of ultrasound to carry out enzymatic reactions, the oscillation amplitude presented a negative effect on yield, whereas pulse factor showed to be benefic for the reactions. |
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2017-03-202017-03-202014-04-16REIS, Cristiane Bianchi Loureiro dos. Production of cellulases by Gelatoporia subvermispora to cellulosic material hydrolysis. 2014. 55 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2014.http://repositorio.ufsm.br/handle/1/5586Several microorganisms are able of producing enzymes responsible for the conversion of biomass to ethanol, such as cellulases. However, studies about diversity and biotechnological application of microorganisms from Pampa biome are still scarce. To try to reduce this knowledge lack, the Gelatoporia subvermispora fungus was isolated from the Pampa biome, resulting in the first occurrence of this specie in Latin America. Commercially, cellulases are produced by submerged fermentation. However, filamentous fungi that are considered good producers of cellulases show better results in solid-state fermentation, mainly by the similarity between the solid medium and the natural habitat of these microorganisms. In order to evaluate the biotechnological potential of the fungi G. subvermispora for cellulases production, rice straw, sewage sludge and sugar cane bagasse were used as substrate. The highest values for the total cellulolytic activity by the method of filter paper (FP) were obtained using sugarcane bagasse as substrate, reaching the value of 3.82 FPU.g-1. The sewage sludge was an excellent medium for the production of xylanase and exo-cellulases, reaching peak activity of 227.97 U.g-1 and 134.25 U.g-1, respectively. The endo-cellulase activity was similar in almost whole substrates tested, as showed in the runs 11 for rice straw (40.75 U.g-1) and 14 for sewage sludge (35.32 U.g-1). Subsequently, cellulolytic enzymes from fungal Gelatoporia subvermispora produced by solid-state fermentation using different substrates were applied to hydrolyze non-treated sugarcane bagasse using indirect sonication. The best results were obtained using sewage sludge as substrate for enzyme production. The mean yields obtained with enzymes produced using sewage sludge, sugarcane bagasse and rice straw as substrates were 72.8, 58.7 and 51.2 g.kg-1, respectively. Regarding the use of ultrasound to carry out enzymatic reactions, the oscillation amplitude presented a negative effect on yield, whereas pulse factor showed to be benefic for the reactions.Diversos microrganismos são capazes de produzir enzimas responsáveis pela conversão da biomassa em etanol, tais como as celulases, porém, estudos sobre a diversidade e aplicação biotecnológica dos microrganismos do bioma Pampa ainda são escassos. Para tentar reduzir essa lacuna de conhecimento, o fungo Gelatoporia subvermispora foi isolado do bioma Pampa, resultando na primeira ocorrência dessa espécie para a América Latina. Comercialmente, as celulases são produzidas através de fermentação submersa. Entretanto, fungos filamentosos que são considerados bons produtores de celulases apresentam melhores resultados em fermentação em estado sólido. Para avaliar o potencial biotecnológico do fungo G. subvermispora de produzir celulases os resíduos palha de arroz, lodo de esgoto e bagaço de cana foram utilizados como substrato. Os maiores valores de atividade celulolítica total determinados pelo método de papel filtro (PF) foram obtidos utilizando bagaço de cana como substrato, apresentando 3.82 FPU.g-1. O lodo de esgoto foi um excelente meio para a produção de xilanases e exo-celulases, atingindo valores máximos de 227.97 U.g-1 e 134.25 U.g-1, respectivamente. A atividade da endo-celulase foi similar nos substratos testados, como verificado na condição 11 para a palha de arroz (40.75 U.g-1) e 14 para o lodo de esgoto (35.32 U.g-1). As enzimas celulolíticas produzidas pelo fungo Gelatoporia subvermispora foram aplicadas na hidrólise do bagaço de cana por meio de sonicação indireta. Os melhores resultados foram obtidos utilizando o lodo de esgoto como substrato para a produção das enzimas. Os rendimentos médios de hidrólise obtidos através da produção de enzimas utilizando lodo de esgoto, bagaço de cana e palha de arroz como substrato foram 72.8, 58.7 e 51.2 g.kg-1, respectivamente. Com relação à utilização do ultrassom para as reações enzimáticas, a amplitude apresentou um efeito negativo no rendimento, enquanto o fator pulso foi benéfico para as reações.application/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciência do SoloUFSMBRAgronomiaFungos filamentososResíduos lignocelulósicosFermentação em estado sólidoEnzimasFilamentous fungiLignocellulosic residuesSolid-state fermentationEnzymesCNPQ::CIENCIAS AGRARIAS::AGRONOMIAProdução de celulases por Gelatoporia subvermispora para hidrólise de material celulósicoProduction of cellulases by Gelatoporia subvermispora to cellulosic material hydrolysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisJacques, Rodrigo Josemar SeminotiMazutti, Marcio Antoniohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4770663T7Salazar, Rodrigo Fernando dos Santoshttp://lattes.cnpq.br/9370462066771109Reis, Cristiane Bianchi Loureiro dos500100000009400500300500300d08ea39d-f039-4bf8-8abb-77396310decbcf1fed7e-741e-4bcd-8ca2-3864f7145db267e239f9-31ad-4d6b-9c76-bea8273292994831ad85-c61b-4bdc-a7fa-70f5c5fc058cinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALREIS, CRISTIANE BIANCHI LOUREIRO DOS.pdfapplication/pdf807855http://repositorio.ufsm.br/bitstream/1/5586/1/REIS%2c%20CRISTIANE%20BIANCHI%20LOUREIRO%20DOS.pdf7c74f333bcb39354f329a910e486d50eMD51TEXTREIS, CRISTIANE BIANCHI LOUREIRO DOS.pdf.txtREIS, CRISTIANE BIANCHI LOUREIRO DOS.pdf.txtExtracted texttext/plain86757http://repositorio.ufsm.br/bitstream/1/5586/2/REIS%2c%20CRISTIANE%20BIANCHI%20LOUREIRO%20DOS.pdf.txt2536a97c354e006ca42f3e35ac393a05MD52THUMBNAILREIS, CRISTIANE BIANCHI LOUREIRO DOS.pdf.jpgREIS, CRISTIANE BIANCHI LOUREIRO DOS.pdf.jpgIM Thumbnailimage/jpeg4799http://repositorio.ufsm.br/bitstream/1/5586/3/REIS%2c%20CRISTIANE%20BIANCHI%20LOUREIRO%20DOS.pdf.jpg586686f2b7e6cd94c3e4a75bf4c6b53fMD531/55862017-07-25 11:17:28.365oai:repositorio.ufsm.br:1/5586Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2017-07-25T14:17:28Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Produção de celulases por Gelatoporia subvermispora para hidrólise de material celulósico |
dc.title.alternative.eng.fl_str_mv |
Production of cellulases by Gelatoporia subvermispora to cellulosic material hydrolysis |
title |
Produção de celulases por Gelatoporia subvermispora para hidrólise de material celulósico |
spellingShingle |
Produção de celulases por Gelatoporia subvermispora para hidrólise de material celulósico Reis, Cristiane Bianchi Loureiro dos Fungos filamentosos Resíduos lignocelulósicos Fermentação em estado sólido Enzimas Filamentous fungi Lignocellulosic residues Solid-state fermentation Enzymes CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Produção de celulases por Gelatoporia subvermispora para hidrólise de material celulósico |
title_full |
Produção de celulases por Gelatoporia subvermispora para hidrólise de material celulósico |
title_fullStr |
Produção de celulases por Gelatoporia subvermispora para hidrólise de material celulósico |
title_full_unstemmed |
Produção de celulases por Gelatoporia subvermispora para hidrólise de material celulósico |
title_sort |
Produção de celulases por Gelatoporia subvermispora para hidrólise de material celulósico |
author |
Reis, Cristiane Bianchi Loureiro dos |
author_facet |
Reis, Cristiane Bianchi Loureiro dos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Jacques, Rodrigo Josemar Seminoti |
dc.contributor.referee1.fl_str_mv |
Mazutti, Marcio Antonio |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4770663T7 |
dc.contributor.referee2.fl_str_mv |
Salazar, Rodrigo Fernando dos Santos |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/9370462066771109 |
dc.contributor.author.fl_str_mv |
Reis, Cristiane Bianchi Loureiro dos |
contributor_str_mv |
Jacques, Rodrigo Josemar Seminoti Mazutti, Marcio Antonio Salazar, Rodrigo Fernando dos Santos |
dc.subject.por.fl_str_mv |
Fungos filamentosos Resíduos lignocelulósicos Fermentação em estado sólido Enzimas |
topic |
Fungos filamentosos Resíduos lignocelulósicos Fermentação em estado sólido Enzimas Filamentous fungi Lignocellulosic residues Solid-state fermentation Enzymes CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
dc.subject.eng.fl_str_mv |
Filamentous fungi Lignocellulosic residues Solid-state fermentation Enzymes |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
Several microorganisms are able of producing enzymes responsible for the conversion of biomass to ethanol, such as cellulases. However, studies about diversity and biotechnological application of microorganisms from Pampa biome are still scarce. To try to reduce this knowledge lack, the Gelatoporia subvermispora fungus was isolated from the Pampa biome, resulting in the first occurrence of this specie in Latin America. Commercially, cellulases are produced by submerged fermentation. However, filamentous fungi that are considered good producers of cellulases show better results in solid-state fermentation, mainly by the similarity between the solid medium and the natural habitat of these microorganisms. In order to evaluate the biotechnological potential of the fungi G. subvermispora for cellulases production, rice straw, sewage sludge and sugar cane bagasse were used as substrate. The highest values for the total cellulolytic activity by the method of filter paper (FP) were obtained using sugarcane bagasse as substrate, reaching the value of 3.82 FPU.g-1. The sewage sludge was an excellent medium for the production of xylanase and exo-cellulases, reaching peak activity of 227.97 U.g-1 and 134.25 U.g-1, respectively. The endo-cellulase activity was similar in almost whole substrates tested, as showed in the runs 11 for rice straw (40.75 U.g-1) and 14 for sewage sludge (35.32 U.g-1). Subsequently, cellulolytic enzymes from fungal Gelatoporia subvermispora produced by solid-state fermentation using different substrates were applied to hydrolyze non-treated sugarcane bagasse using indirect sonication. The best results were obtained using sewage sludge as substrate for enzyme production. The mean yields obtained with enzymes produced using sewage sludge, sugarcane bagasse and rice straw as substrates were 72.8, 58.7 and 51.2 g.kg-1, respectively. Regarding the use of ultrasound to carry out enzymatic reactions, the oscillation amplitude presented a negative effect on yield, whereas pulse factor showed to be benefic for the reactions. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-04-16 |
dc.date.accessioned.fl_str_mv |
2017-03-20 |
dc.date.available.fl_str_mv |
2017-03-20 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
REIS, Cristiane Bianchi Loureiro dos. Production of cellulases by Gelatoporia subvermispora to cellulosic material hydrolysis. 2014. 55 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/5586 |
identifier_str_mv |
REIS, Cristiane Bianchi Loureiro dos. Production of cellulases by Gelatoporia subvermispora to cellulosic material hydrolysis. 2014. 55 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2014. |
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http://repositorio.ufsm.br/handle/1/5586 |
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por |
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