Distúrbios hemostáticos e atividade das enzimas que hidrolisam nucleotídeos e nucleosídeo de adenina em cães infectados com Rangelia vitalii
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/4075 |
Resumo: | The parasite Rangelia vitalii is the etiologic agent of rangeliosis, a disease that leads to a wide variety of clinical signs, including hemostatic disorders characterized by bleeding. However, the causes of this process have not been established. The aim of this study was to evaluate the production of megakaryocytes, platelet count, clotting time, platelet activity and to determine the activity of enzymes that hydrolyze nucleotides and adenine nucleosides in platelets of dogs experimentally infected with R. vitalii. For this study, 12 dogs were separated in two groups: Group A was composed by five healthy dogs, and group B composed by seven dogs experimentally infected with R. vitalii. After inoculation, the animals were monitored by blood smears. The parasite was found within erythrocytes, neutrophils and monocytes five days post-inoculation (PI). Blood collection to perform platelet count, coagulation tests and measurement of platelet aggregation was performed on days 0, 10 and 20 PI. On days 10 and 20 PI, after this procedure, the dogs were anesthetized for collecting bone marrow samples to evaluate the production of megakaryocytes. To measure the activity of ectonucleotidases and adenosine deaminase, blood samples were collected on days 12 and 21 PI. Blood samples were stored in tubes containing EDTA to quantification of platelets, evaluation of platelet aggregation and measurement of enzymatic activity. The blood was stored in tubes containing citrate for realization of the clotting time. This study revealed a reduction (P<0.01) of platelet numbers in the infected group when compared to control group. Prothrombin time and active partial thromboplastin time showed no significant difference between infected and control animals. There was an increase (P<0.01) in the number of megakaryocytes in infected group when compared with control group. A reduction (P<0.01) of platelet aggregation was observed in dogs infected with R. vitalii. The hydrolysis of ATP, ADP, AMP and deamination of adenosine decreased (P<0.01) on day 12 PI. On day 21 PI, the activity of adenosine deaminase remained reduced (P<0.01), whereas it was observed an increase (P<0.05) in NTPDase levels. From these results, we can conclude that rangeliosis is responsible for severe thrombocytopenia during the acute phase of infection, and this can be due to splenic sequestration and/or immune-mediated thrombocytopenia. Also, alterations in purinergic system contribute to the occurrence of hemostatic disorders observed in this disease. |
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Distúrbios hemostáticos e atividade das enzimas que hidrolisam nucleotídeos e nucleosídeo de adenina em cães infectados com Rangelia vitaliiRangelioseMegacariócitosTrombocitopeniaCoagulaçãoAdenosinaEctonucleotidasesAdenosina desaminaseRangeliosisMegakaryocytesThrombocytopeniaCoagulationAdenosineCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAThe parasite Rangelia vitalii is the etiologic agent of rangeliosis, a disease that leads to a wide variety of clinical signs, including hemostatic disorders characterized by bleeding. However, the causes of this process have not been established. The aim of this study was to evaluate the production of megakaryocytes, platelet count, clotting time, platelet activity and to determine the activity of enzymes that hydrolyze nucleotides and adenine nucleosides in platelets of dogs experimentally infected with R. vitalii. For this study, 12 dogs were separated in two groups: Group A was composed by five healthy dogs, and group B composed by seven dogs experimentally infected with R. vitalii. After inoculation, the animals were monitored by blood smears. The parasite was found within erythrocytes, neutrophils and monocytes five days post-inoculation (PI). Blood collection to perform platelet count, coagulation tests and measurement of platelet aggregation was performed on days 0, 10 and 20 PI. On days 10 and 20 PI, after this procedure, the dogs were anesthetized for collecting bone marrow samples to evaluate the production of megakaryocytes. To measure the activity of ectonucleotidases and adenosine deaminase, blood samples were collected on days 12 and 21 PI. Blood samples were stored in tubes containing EDTA to quantification of platelets, evaluation of platelet aggregation and measurement of enzymatic activity. The blood was stored in tubes containing citrate for realization of the clotting time. This study revealed a reduction (P<0.01) of platelet numbers in the infected group when compared to control group. Prothrombin time and active partial thromboplastin time showed no significant difference between infected and control animals. There was an increase (P<0.01) in the number of megakaryocytes in infected group when compared with control group. A reduction (P<0.01) of platelet aggregation was observed in dogs infected with R. vitalii. The hydrolysis of ATP, ADP, AMP and deamination of adenosine decreased (P<0.01) on day 12 PI. On day 21 PI, the activity of adenosine deaminase remained reduced (P<0.01), whereas it was observed an increase (P<0.05) in NTPDase levels. From these results, we can conclude that rangeliosis is responsible for severe thrombocytopenia during the acute phase of infection, and this can be due to splenic sequestration and/or immune-mediated thrombocytopenia. Also, alterations in purinergic system contribute to the occurrence of hemostatic disorders observed in this disease.O parasito Rangelia vitalii é o agente etiológico da rangeliose, uma enfermidade que cursa com uma grande variedade de sinais clínicos, incluindo desordens hemostáticas caracterizadas por sangramentos. Entretanto, as causas das alterações no processo hemostático nessa doença ainda não foram esclarecidas. O objetivo deste estudo foi avaliar os distúrbios hemostáticos através da produção de megacariócitos e contagem de plaquetas, a coagulação sanguínea, a atividade plaquetária e determinar a atividade das enzimas que hidrolisam nucleotídeos e nucleosídeo de adenina em plaquetas de cães infectados experimentalmente com R. vitalii. Para este estudo, 12 cães foram separados em dois grupos: Grupo A foi composto por cinco cães saudáveis, e grupo B com sete cães infectados experimentalmente com R. vitalii. Após inoculação, os animais foram monitorados quanto à parasitemia por esfregaço sanguíneo. O parasito foi encontrado no interior de hemácias, neutrófilos e monócitos cinco dias pós-inoculação (PI). As coletas de sangue para a realização da contagem plaquetária, testes de coagulação e mensuração da agregação plaquetária foram realizadas nos dias 0, 10 e 20 PI. Nos dias 10 e 20 PI, após esse procedimento, os cães foram anestesiados para coleta do aspirado de medula óssea com a finalidade de avaliar a produção de megacariócitos. Para avaliar a atividade das ectonucleotidases e adenosina desaminase, as coletas de sangue foram realizadas nos dias 12 e 21 PI. Este estudo demonstrou redução (P<0,01) no número de plaquetas entre o grupo infectado em relação ao controle. Os tempos de protrombina e de tromboplastina parcial ativado não apresentaram diferença significativa entre animais infectados e controles. Ocorreu aumento (P<0,01) do número de megacariócitos no grupo infectado quando comparado com o controle. Foi observado uma diminuição (P<0,01) na agregação plaquetária em cães infectados por R. vitalii. A hidrólise de ATP, ADP, AMP e desaminação da adenosina foram reduzidas (P<0,01) no dia 12 PI quando comparadas com o grupo controle. No dia 21 PI, a atividade da adenosina desaminase manteve-se reduzida (P<0,01), enquanto que ocorreu aumento (P<0,05) na atividade da ecto-nucleosídeo trifosfato difosfoidrolase (NTPDase). A partir dos resultados, pode-se concluir que a rangeliose é responsável por grave trombocitopenia na fase aguda da infecção, a qual pode estar associada ao sequestro e/ou destruição imunomediada e, que alterações no sistema purinérgico contribuem para a ocorrência das desordens hemostáticas observadas na infecção pelo parasito R. vitalii.Universidade Federal de Santa MariaBRMedicina VeterináriaUFSMPrograma de Pós-Graduação em Medicina VeterináriaLopes, Sonia Terezinha dos Anjoshttp://lattes.cnpq.br/8059723754130756Monteiro, Silvia Gonzalezhttp://lattes.cnpq.br/3762606653182779Krause, Alexandrehttp://lattes.cnpq.br/7760558908777387Duarte, Marta Maria Medeiros Frescurahttp://lattes.cnpq.br/6277584896102052Veiga, Angela Patricia Medeiroshttp://lattes.cnpq.br/7237637146454629Paim, Carlos Breno Viana2017-05-302017-05-302012-08-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfapplication/pdfPAIM, Carlos Breno Viana. Distúrbios hemostáticos e atividade das enzimas que hidrolisam nucleotídeos e nucleosídeo de adenina em cães infectados com Rangelia vitalii. 2012. 65 f. Tese (Doutorado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2012.http://repositorio.ufsm.br/handle/1/4075porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2023-06-21T14:53:04Zoai:repositorio.ufsm.br:1/4075Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-06-21T14:53:04Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Distúrbios hemostáticos e atividade das enzimas que hidrolisam nucleotídeos e nucleosídeo de adenina em cães infectados com Rangelia vitalii |
title |
Distúrbios hemostáticos e atividade das enzimas que hidrolisam nucleotídeos e nucleosídeo de adenina em cães infectados com Rangelia vitalii |
spellingShingle |
Distúrbios hemostáticos e atividade das enzimas que hidrolisam nucleotídeos e nucleosídeo de adenina em cães infectados com Rangelia vitalii Paim, Carlos Breno Viana Rangeliose Megacariócitos Trombocitopenia Coagulação Adenosina Ectonucleotidases Adenosina desaminase Rangeliosis Megakaryocytes Thrombocytopenia Coagulation Adenosine CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Distúrbios hemostáticos e atividade das enzimas que hidrolisam nucleotídeos e nucleosídeo de adenina em cães infectados com Rangelia vitalii |
title_full |
Distúrbios hemostáticos e atividade das enzimas que hidrolisam nucleotídeos e nucleosídeo de adenina em cães infectados com Rangelia vitalii |
title_fullStr |
Distúrbios hemostáticos e atividade das enzimas que hidrolisam nucleotídeos e nucleosídeo de adenina em cães infectados com Rangelia vitalii |
title_full_unstemmed |
Distúrbios hemostáticos e atividade das enzimas que hidrolisam nucleotídeos e nucleosídeo de adenina em cães infectados com Rangelia vitalii |
title_sort |
Distúrbios hemostáticos e atividade das enzimas que hidrolisam nucleotídeos e nucleosídeo de adenina em cães infectados com Rangelia vitalii |
author |
Paim, Carlos Breno Viana |
author_facet |
Paim, Carlos Breno Viana |
author_role |
author |
dc.contributor.none.fl_str_mv |
Lopes, Sonia Terezinha dos Anjos http://lattes.cnpq.br/8059723754130756 Monteiro, Silvia Gonzalez http://lattes.cnpq.br/3762606653182779 Krause, Alexandre http://lattes.cnpq.br/7760558908777387 Duarte, Marta Maria Medeiros Frescura http://lattes.cnpq.br/6277584896102052 Veiga, Angela Patricia Medeiros http://lattes.cnpq.br/7237637146454629 |
dc.contributor.author.fl_str_mv |
Paim, Carlos Breno Viana |
dc.subject.por.fl_str_mv |
Rangeliose Megacariócitos Trombocitopenia Coagulação Adenosina Ectonucleotidases Adenosina desaminase Rangeliosis Megakaryocytes Thrombocytopenia Coagulation Adenosine CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
topic |
Rangeliose Megacariócitos Trombocitopenia Coagulação Adenosina Ectonucleotidases Adenosina desaminase Rangeliosis Megakaryocytes Thrombocytopenia Coagulation Adenosine CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The parasite Rangelia vitalii is the etiologic agent of rangeliosis, a disease that leads to a wide variety of clinical signs, including hemostatic disorders characterized by bleeding. However, the causes of this process have not been established. The aim of this study was to evaluate the production of megakaryocytes, platelet count, clotting time, platelet activity and to determine the activity of enzymes that hydrolyze nucleotides and adenine nucleosides in platelets of dogs experimentally infected with R. vitalii. For this study, 12 dogs were separated in two groups: Group A was composed by five healthy dogs, and group B composed by seven dogs experimentally infected with R. vitalii. After inoculation, the animals were monitored by blood smears. The parasite was found within erythrocytes, neutrophils and monocytes five days post-inoculation (PI). Blood collection to perform platelet count, coagulation tests and measurement of platelet aggregation was performed on days 0, 10 and 20 PI. On days 10 and 20 PI, after this procedure, the dogs were anesthetized for collecting bone marrow samples to evaluate the production of megakaryocytes. To measure the activity of ectonucleotidases and adenosine deaminase, blood samples were collected on days 12 and 21 PI. Blood samples were stored in tubes containing EDTA to quantification of platelets, evaluation of platelet aggregation and measurement of enzymatic activity. The blood was stored in tubes containing citrate for realization of the clotting time. This study revealed a reduction (P<0.01) of platelet numbers in the infected group when compared to control group. Prothrombin time and active partial thromboplastin time showed no significant difference between infected and control animals. There was an increase (P<0.01) in the number of megakaryocytes in infected group when compared with control group. A reduction (P<0.01) of platelet aggregation was observed in dogs infected with R. vitalii. The hydrolysis of ATP, ADP, AMP and deamination of adenosine decreased (P<0.01) on day 12 PI. On day 21 PI, the activity of adenosine deaminase remained reduced (P<0.01), whereas it was observed an increase (P<0.05) in NTPDase levels. From these results, we can conclude that rangeliosis is responsible for severe thrombocytopenia during the acute phase of infection, and this can be due to splenic sequestration and/or immune-mediated thrombocytopenia. Also, alterations in purinergic system contribute to the occurrence of hemostatic disorders observed in this disease. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-08-17 2017-05-30 2017-05-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
PAIM, Carlos Breno Viana. Distúrbios hemostáticos e atividade das enzimas que hidrolisam nucleotídeos e nucleosídeo de adenina em cães infectados com Rangelia vitalii. 2012. 65 f. Tese (Doutorado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2012. http://repositorio.ufsm.br/handle/1/4075 |
identifier_str_mv |
PAIM, Carlos Breno Viana. Distúrbios hemostáticos e atividade das enzimas que hidrolisam nucleotídeos e nucleosídeo de adenina em cães infectados com Rangelia vitalii. 2012. 65 f. Tese (Doutorado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
url |
http://repositorio.ufsm.br/handle/1/4075 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Medicina Veterinária UFSM Programa de Pós-Graduação em Medicina Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Medicina Veterinária UFSM Programa de Pós-Graduação em Medicina Veterinária |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
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1805922128129687552 |