Extrato de semente de Syzygium cumini (L.) Skeels reduz o dano renal e hepático provocado pela exposição aguda ao metilmercúrio em ratos neonatos

Detalhes bibliográficos
Autor(a) principal: Abdalla, Faida Husein
Data de Publicação: 2010
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/001300000h11k
Texto Completo: http://repositorio.ufsm.br/handle/1/5918
Resumo: Methylmercury (MeHg) is a potent neuro and nephrotoxicant in several animal species including humans, particularly during their development. The purpose of this study was to investigate the effects of aqueous seed extract of Syzygium cumini (L.) Skeels (Scc) on the acute MeHg treatment in neonatal rats. Neonatal rats (P2) received orally a single dose of MeHg (10 mg/kg) and also two doses of Scc. After two days, the effects of this treatment were investigated in the cerebral cortex, hippocampus, kidney, liver and urine samples of rats. We observed that N-Acetyl-β-d-glucosaminidase (NAG) activity in the kidney and urine was higher in MeHg-group when compared with the control group. Similarly, the lipid peroxidation levels were higher in the liver and kidney as well as the Adenosine deaminase (ADA) activity increased in the hippocampus, kidney and liver. These results indicate that increased NAG and ADA activities, as well as thiobarbituric acid reactive species (TBARS) levels may play a critical role in MeHg nephrotoxicity. The most relevant finding in our investigation was that acute MeHg treatment in neonatal rats caused liver and renal impairment and Scc was able to prevent such effects. It appears that mechanisms related to scavenging activity of Scc could be involved with its protection effect. Key words: Methylmercury; Syzygium cumini; N-Acetyl-β-d-glucosaminidase; rat; adenosine deaminase
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spelling Extrato de semente de Syzygium cumini (L.) Skeels reduz o dano renal e hepático provocado pela exposição aguda ao metilmercúrio em ratos neonatosSyzygium cumini seed extract prevent the renal and liver impairment caused by acute methylmercury treatment in neonatal ratsMetilmercúrioSyzygium cu-d-glucosaminidaseminiN-acetil-β-d-glucosaminidaseRatosAdenosina deaminaseMethylmercurySyzygium cuminiN-Acetyl-β-d-glucosaminidaseRatAdenosine deaminaseCNPQ::CIENCIAS DA SAUDE::FARMACIAMethylmercury (MeHg) is a potent neuro and nephrotoxicant in several animal species including humans, particularly during their development. The purpose of this study was to investigate the effects of aqueous seed extract of Syzygium cumini (L.) Skeels (Scc) on the acute MeHg treatment in neonatal rats. Neonatal rats (P2) received orally a single dose of MeHg (10 mg/kg) and also two doses of Scc. After two days, the effects of this treatment were investigated in the cerebral cortex, hippocampus, kidney, liver and urine samples of rats. We observed that N-Acetyl-β-d-glucosaminidase (NAG) activity in the kidney and urine was higher in MeHg-group when compared with the control group. Similarly, the lipid peroxidation levels were higher in the liver and kidney as well as the Adenosine deaminase (ADA) activity increased in the hippocampus, kidney and liver. These results indicate that increased NAG and ADA activities, as well as thiobarbituric acid reactive species (TBARS) levels may play a critical role in MeHg nephrotoxicity. The most relevant finding in our investigation was that acute MeHg treatment in neonatal rats caused liver and renal impairment and Scc was able to prevent such effects. It appears that mechanisms related to scavenging activity of Scc could be involved with its protection effect. Key words: Methylmercury; Syzygium cumini; N-Acetyl-β-d-glucosaminidase; rat; adenosine deaminaseO metilmercúrio (MeHg) é um agente tóxico potente tanto para o sistema nervoso central como para o renal. Provoca danos nos seres humanos e em ratos, particularmente durante o estágio de desenvolvimento. Neste estudo, ratos em desenvolvimento (P2) receberam uma dose única de MeHg (10 mg/kg), por via oral e/ou duas doses do extrato aquoso de sementes de Syzygium cumini (L.) Skeels (Scc). Após dois dias (P4), foram investigados os efeitos deste tratamento no córtex cerebral, hipocampo, rim, fígado e urina. Observamos que a atividade da N-acetil-β-d-glicosaminidase (NAG) nos rins e na urina foi maior no grupo que recebeu MeHg, quando comparado com o grupo controle. Da mesma forma, os níveis de peroxidação lipídica foram maiores no fígado e rim e a atividade da adenosina deaminase (ADA) encontrou-se elevada no hipocampo, rins e fígado, nos ratos tratados com MeHg. Estes resultados indicam que o aumento da atividade da NAG e da ADA, bem como os níveis das espécies reativas ao ácido tiobarbitúrico (TBARS), desempenham um papel importante como marcadores de nefrotoxicidade causada pelo MeHg. Assim, o achado mais relevante na nossa investigação foi a de que o tratamento agudo com MeHg em ratos neonatos pode provocar nefrotoxicidade e a administração do Scc pode reverter estes efeitos provavelmente devido as propriedades antioxidantes de Scc.Universidade Federal de Santa MariaBRAnálises Clínicas e ToxicológicasUFSMPrograma de Pós-Graduação em Ciências FarmacêuticasMoretto, Maria Beatrizhttp://lattes.cnpq.br/7317262818918502Posser, Thaishttp://lattes.cnpq.br/2277857386983441Franco, Jeferson Luishttp://lattes.cnpq.br/1680065573338339Abdalla, Faida Husein2013-05-022013-05-022010-01-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfABDALLA, Faida Husein. SYZYGIUM CUMINI SEED EXTRACT PREVENT THE RENAL AND LIVER IMPAIRMENT CAUSED BY ACUTE METHYLMERCURY TREATMENT IN NEONATAL RATS. 2010. 80 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2010.http://repositorio.ufsm.br/handle/1/5918ark:/26339/001300000h11kporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-10-10T14:49:56Zoai:repositorio.ufsm.br:1/5918Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-10-10T14:49:56Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Extrato de semente de Syzygium cumini (L.) Skeels reduz o dano renal e hepático provocado pela exposição aguda ao metilmercúrio em ratos neonatos
Syzygium cumini seed extract prevent the renal and liver impairment caused by acute methylmercury treatment in neonatal rats
title Extrato de semente de Syzygium cumini (L.) Skeels reduz o dano renal e hepático provocado pela exposição aguda ao metilmercúrio em ratos neonatos
spellingShingle Extrato de semente de Syzygium cumini (L.) Skeels reduz o dano renal e hepático provocado pela exposição aguda ao metilmercúrio em ratos neonatos
Abdalla, Faida Husein
Metilmercúrio
Syzygium cu-d-glucosaminidasemini
N-acetil-β-d-glucosaminidase
Ratos
Adenosina deaminase
Methylmercury
Syzygium cumini
N-Acetyl-β-d-glucosaminidase
Rat
Adenosine deaminase
CNPQ::CIENCIAS DA SAUDE::FARMACIA
title_short Extrato de semente de Syzygium cumini (L.) Skeels reduz o dano renal e hepático provocado pela exposição aguda ao metilmercúrio em ratos neonatos
title_full Extrato de semente de Syzygium cumini (L.) Skeels reduz o dano renal e hepático provocado pela exposição aguda ao metilmercúrio em ratos neonatos
title_fullStr Extrato de semente de Syzygium cumini (L.) Skeels reduz o dano renal e hepático provocado pela exposição aguda ao metilmercúrio em ratos neonatos
title_full_unstemmed Extrato de semente de Syzygium cumini (L.) Skeels reduz o dano renal e hepático provocado pela exposição aguda ao metilmercúrio em ratos neonatos
title_sort Extrato de semente de Syzygium cumini (L.) Skeels reduz o dano renal e hepático provocado pela exposição aguda ao metilmercúrio em ratos neonatos
author Abdalla, Faida Husein
author_facet Abdalla, Faida Husein
author_role author
dc.contributor.none.fl_str_mv Moretto, Maria Beatriz
http://lattes.cnpq.br/7317262818918502
Posser, Thais
http://lattes.cnpq.br/2277857386983441
Franco, Jeferson Luis
http://lattes.cnpq.br/1680065573338339
dc.contributor.author.fl_str_mv Abdalla, Faida Husein
dc.subject.por.fl_str_mv Metilmercúrio
Syzygium cu-d-glucosaminidasemini
N-acetil-β-d-glucosaminidase
Ratos
Adenosina deaminase
Methylmercury
Syzygium cumini
N-Acetyl-β-d-glucosaminidase
Rat
Adenosine deaminase
CNPQ::CIENCIAS DA SAUDE::FARMACIA
topic Metilmercúrio
Syzygium cu-d-glucosaminidasemini
N-acetil-β-d-glucosaminidase
Ratos
Adenosina deaminase
Methylmercury
Syzygium cumini
N-Acetyl-β-d-glucosaminidase
Rat
Adenosine deaminase
CNPQ::CIENCIAS DA SAUDE::FARMACIA
description Methylmercury (MeHg) is a potent neuro and nephrotoxicant in several animal species including humans, particularly during their development. The purpose of this study was to investigate the effects of aqueous seed extract of Syzygium cumini (L.) Skeels (Scc) on the acute MeHg treatment in neonatal rats. Neonatal rats (P2) received orally a single dose of MeHg (10 mg/kg) and also two doses of Scc. After two days, the effects of this treatment were investigated in the cerebral cortex, hippocampus, kidney, liver and urine samples of rats. We observed that N-Acetyl-β-d-glucosaminidase (NAG) activity in the kidney and urine was higher in MeHg-group when compared with the control group. Similarly, the lipid peroxidation levels were higher in the liver and kidney as well as the Adenosine deaminase (ADA) activity increased in the hippocampus, kidney and liver. These results indicate that increased NAG and ADA activities, as well as thiobarbituric acid reactive species (TBARS) levels may play a critical role in MeHg nephrotoxicity. The most relevant finding in our investigation was that acute MeHg treatment in neonatal rats caused liver and renal impairment and Scc was able to prevent such effects. It appears that mechanisms related to scavenging activity of Scc could be involved with its protection effect. Key words: Methylmercury; Syzygium cumini; N-Acetyl-β-d-glucosaminidase; rat; adenosine deaminase
publishDate 2010
dc.date.none.fl_str_mv 2010-01-25
2013-05-02
2013-05-02
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv ABDALLA, Faida Husein. SYZYGIUM CUMINI SEED EXTRACT PREVENT THE RENAL AND LIVER IMPAIRMENT CAUSED BY ACUTE METHYLMERCURY TREATMENT IN NEONATAL RATS. 2010. 80 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2010.
http://repositorio.ufsm.br/handle/1/5918
dc.identifier.dark.fl_str_mv ark:/26339/001300000h11k
identifier_str_mv ABDALLA, Faida Husein. SYZYGIUM CUMINI SEED EXTRACT PREVENT THE RENAL AND LIVER IMPAIRMENT CAUSED BY ACUTE METHYLMERCURY TREATMENT IN NEONATAL RATS. 2010. 80 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2010.
ark:/26339/001300000h11k
url http://repositorio.ufsm.br/handle/1/5918
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Análises Clínicas e Toxicológicas
UFSM
Programa de Pós-Graduação em Ciências Farmacêuticas
publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Análises Clínicas e Toxicológicas
UFSM
Programa de Pós-Graduação em Ciências Farmacêuticas
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
_version_ 1815172342307880960

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