Resposta morfo-adaptativa e antioxidante de jundiá (Rhamdia quelen) suplementados com nanoemulsão de Cymbopogon flexuosus na dieta
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/28866 |
Resumo: | The effect of Cymbopogon flexuosus essential oil nanoemulsion on morpho-adaptive response and its antioxidant potential in silver catfish (Rhamdia quelen) naturally infected by Aeromonas hydrophila was studied. For this, silver catfish (n= 400) were randomly distributed in a water recirculation system consisting of 20 polypropylene boxes, 20 fish per box (4 replicates each treatment). Dietary treatments consisted of five experimental diets supplemented with OECF nanoemulsion at the following concentrations: 0; 0.25; 0.5; 1.0 and 2.0 mL/kg of feed. For Twenty-one days, the animals were fed twice a day (09:00 and 19:00 hours) and the parameters of water quality, temperature, dissolved oxygen, pH and ammonia were monitored. At the end of the experiment, silver catfish (n= 8) were anesthetized and euthanized to collect biological material. Liver, gill and skin samples were carefully collected for further morphometric and oxidative state analyses. The variables of water quality remained within the range considered ideal for the espécies. The histomorphometric results of gills and skin showed that the highest levels of nanoemulsion of OECF (1.0 and 2.0 mL/kg of feed) have no effect on skin and gill morphometry of silver catfish naturally infected by A. hydrophila. However, the dietary supplementation of OECF nanoemultion at the concentration of 0.5 m/kg feed showed antioxidant effect on the gills of silver catfish naturally infected by A. hydrophila. Silver catfish naturally affected by A. hydrophila presented structures of the normal hepatic parenchyma, indicating thet the nanoemulsion of OECF has a hepatoprotective effect against infection. Thus, the beneficial effects observed in this study indicate the promising use of OECF nanoemulsion in aquaculture. |
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Resposta morfo-adaptativa e antioxidante de jundiá (Rhamdia quelen) suplementados com nanoemulsão de Cymbopogon flexuosus na dietaMorph-adaptive and antioxidant response of silver catfish (Rhamdia quelen) supplemented with Cymvopogon flexuosus nanoemulsion in dietAditivo alimentarCélulas mucosasEstresse oxidativoMorfometria da pele e brânquiasFood aditiveMucous cellsOxidative stressSkin and gill morphometryCNPQ::CIENCIAS AGRARIAS::ZOOTECNIAThe effect of Cymbopogon flexuosus essential oil nanoemulsion on morpho-adaptive response and its antioxidant potential in silver catfish (Rhamdia quelen) naturally infected by Aeromonas hydrophila was studied. For this, silver catfish (n= 400) were randomly distributed in a water recirculation system consisting of 20 polypropylene boxes, 20 fish per box (4 replicates each treatment). Dietary treatments consisted of five experimental diets supplemented with OECF nanoemulsion at the following concentrations: 0; 0.25; 0.5; 1.0 and 2.0 mL/kg of feed. For Twenty-one days, the animals were fed twice a day (09:00 and 19:00 hours) and the parameters of water quality, temperature, dissolved oxygen, pH and ammonia were monitored. At the end of the experiment, silver catfish (n= 8) were anesthetized and euthanized to collect biological material. Liver, gill and skin samples were carefully collected for further morphometric and oxidative state analyses. The variables of water quality remained within the range considered ideal for the espécies. The histomorphometric results of gills and skin showed that the highest levels of nanoemulsion of OECF (1.0 and 2.0 mL/kg of feed) have no effect on skin and gill morphometry of silver catfish naturally infected by A. hydrophila. However, the dietary supplementation of OECF nanoemultion at the concentration of 0.5 m/kg feed showed antioxidant effect on the gills of silver catfish naturally infected by A. hydrophila. Silver catfish naturally affected by A. hydrophila presented structures of the normal hepatic parenchyma, indicating thet the nanoemulsion of OECF has a hepatoprotective effect against infection. Thus, the beneficial effects observed in this study indicate the promising use of OECF nanoemulsion in aquaculture.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESO efeito da nanoemulsão do óleo essencial (OE) de Cymbopogon flexuosus na resposta morfoadaptativa e seu potencial antioxidante em jundiá (Rhamdia quelen) naturalmente infectados por Aeromonas hydrophila foi estudado. Para isso, jundiás (n= 400) foram distribuídos aleatoriamente em sistema de recirculação de água constituído de 20 caixas de polipropileno, 20 peixes por caixa (4 repetições cada tratamento). Os tratamentos dietéticos foram constituídos de cinco dietas experimentais suplementadas com nanoemulsão do OECF nas seguintes concentrações: 0; 0.25; 0.5; 1.0 e 2.0 mL/kg de ração. Durante vinte e um dias, os animais foram alimentados duas vezes ao dia (09:00 e 19:00 horas) e os parâmetros de qualidade da água, temperatura, oxigênio dissolvido, pH e amônia foram monitorados. Ao final do experimento, jundiás (n= 8), foram anestesiados e eutanasiados para coleta de material biológico. Amostras de fígado, brânquias e pele foram cuidadosamente coletadas para posteriores análises morfométricas e do estado oxidativo. As variáveis de qualidade da água mantiveram-se dentro da faixa considerada ideal para a espécie. Os resultados histomorfométricos de brânquias e pele demonstraram que os maiores níveis de nanoemulsão do OECF (1.0 e 2.0 mL/Kg de ração), não possuem efeito sobre a morfometria cutânea e branquial de jundiá naturalmente infectados por A. hydrophila. Porém, a suplementação dietética da nanoemulsão do OECF na concentração de 0.5 mL/kg de ração demonstrou efeito antioxidante nas brânquias de jundiás naturalmente infectados por A. hydrophila. Jundiás acometidos naturalmente por A. hydrophila apresentaram estruturas do parênquima hepático normal, indicando que a nanoemulsão de OECF possui efeito hepatoprotetor contra infecção. Deste modo, os efeitos benéficos observados neste estudo indicam o uso promissor da nanoemulsão do OECF na aquicultura.Universidade Federal de Santa MariaBrasilZootecniaUFSMPrograma de Pós-Graduação em ZootecniaCentro de Ciências RuraisCosta, Sílvio Teixeira dahttp://lattes.cnpq.br/7580918019716877Baldisserotto, BernardoSutili, Fernando JonasVeiga, Marcelo Leite daValerio Junior, José Francisco2023-04-28T11:49:27Z2023-04-28T11:49:27Z2023-02-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/28866porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2023-04-28T11:49:27Zoai:repositorio.ufsm.br:1/28866Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-04-28T11:49:27Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Resposta morfo-adaptativa e antioxidante de jundiá (Rhamdia quelen) suplementados com nanoemulsão de Cymbopogon flexuosus na dieta Morph-adaptive and antioxidant response of silver catfish (Rhamdia quelen) supplemented with Cymvopogon flexuosus nanoemulsion in diet |
title |
Resposta morfo-adaptativa e antioxidante de jundiá (Rhamdia quelen) suplementados com nanoemulsão de Cymbopogon flexuosus na dieta |
spellingShingle |
Resposta morfo-adaptativa e antioxidante de jundiá (Rhamdia quelen) suplementados com nanoemulsão de Cymbopogon flexuosus na dieta Valerio Junior, José Francisco Aditivo alimentar Células mucosas Estresse oxidativo Morfometria da pele e brânquias Food aditive Mucous cells Oxidative stress Skin and gill morphometry CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA |
title_short |
Resposta morfo-adaptativa e antioxidante de jundiá (Rhamdia quelen) suplementados com nanoemulsão de Cymbopogon flexuosus na dieta |
title_full |
Resposta morfo-adaptativa e antioxidante de jundiá (Rhamdia quelen) suplementados com nanoemulsão de Cymbopogon flexuosus na dieta |
title_fullStr |
Resposta morfo-adaptativa e antioxidante de jundiá (Rhamdia quelen) suplementados com nanoemulsão de Cymbopogon flexuosus na dieta |
title_full_unstemmed |
Resposta morfo-adaptativa e antioxidante de jundiá (Rhamdia quelen) suplementados com nanoemulsão de Cymbopogon flexuosus na dieta |
title_sort |
Resposta morfo-adaptativa e antioxidante de jundiá (Rhamdia quelen) suplementados com nanoemulsão de Cymbopogon flexuosus na dieta |
author |
Valerio Junior, José Francisco |
author_facet |
Valerio Junior, José Francisco |
author_role |
author |
dc.contributor.none.fl_str_mv |
Costa, Sílvio Teixeira da http://lattes.cnpq.br/7580918019716877 Baldisserotto, Bernardo Sutili, Fernando Jonas Veiga, Marcelo Leite da |
dc.contributor.author.fl_str_mv |
Valerio Junior, José Francisco |
dc.subject.por.fl_str_mv |
Aditivo alimentar Células mucosas Estresse oxidativo Morfometria da pele e brânquias Food aditive Mucous cells Oxidative stress Skin and gill morphometry CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA |
topic |
Aditivo alimentar Células mucosas Estresse oxidativo Morfometria da pele e brânquias Food aditive Mucous cells Oxidative stress Skin and gill morphometry CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA |
description |
The effect of Cymbopogon flexuosus essential oil nanoemulsion on morpho-adaptive response and its antioxidant potential in silver catfish (Rhamdia quelen) naturally infected by Aeromonas hydrophila was studied. For this, silver catfish (n= 400) were randomly distributed in a water recirculation system consisting of 20 polypropylene boxes, 20 fish per box (4 replicates each treatment). Dietary treatments consisted of five experimental diets supplemented with OECF nanoemulsion at the following concentrations: 0; 0.25; 0.5; 1.0 and 2.0 mL/kg of feed. For Twenty-one days, the animals were fed twice a day (09:00 and 19:00 hours) and the parameters of water quality, temperature, dissolved oxygen, pH and ammonia were monitored. At the end of the experiment, silver catfish (n= 8) were anesthetized and euthanized to collect biological material. Liver, gill and skin samples were carefully collected for further morphometric and oxidative state analyses. The variables of water quality remained within the range considered ideal for the espécies. The histomorphometric results of gills and skin showed that the highest levels of nanoemulsion of OECF (1.0 and 2.0 mL/kg of feed) have no effect on skin and gill morphometry of silver catfish naturally infected by A. hydrophila. However, the dietary supplementation of OECF nanoemultion at the concentration of 0.5 m/kg feed showed antioxidant effect on the gills of silver catfish naturally infected by A. hydrophila. Silver catfish naturally affected by A. hydrophila presented structures of the normal hepatic parenchyma, indicating thet the nanoemulsion of OECF has a hepatoprotective effect against infection. Thus, the beneficial effects observed in this study indicate the promising use of OECF nanoemulsion in aquaculture. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-04-28T11:49:27Z 2023-04-28T11:49:27Z 2023-02-08 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/28866 |
url |
http://repositorio.ufsm.br/handle/1/28866 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Zootecnia UFSM Programa de Pós-Graduação em Zootecnia Centro de Ciências Rurais |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Zootecnia UFSM Programa de Pós-Graduação em Zootecnia Centro de Ciências Rurais |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922042090881024 |